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All Journal MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Biotika: Jurnal Ilmiah Biologi Bioscience Microbiology Indonesia AL KAUNIYAH Jurnal Sains dan Teknologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biosfer: Jurnal Tadris Biologi Biogenesis: Jurnal Ilmiah Biologi Quagga Gontor AGROTECH Science Journal Biology, Medicine, & Natural Product Chemistry Journal of Natural Sciences and Mathematics Research Jurnal Biodjati Jurnal Pengabdian Pada Masyarakat JRST (Jurnal Riset Sains dan Teknologi) Biosel: Biologi Science and Education Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi KACANEGARA Jurnal Pengabdian pada Masyarakat AL-HAYAT: Journal of Biology and Applied Biology Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Canrea Journal: Food Technology, Nutritions, and Culinary Journal Kontribusia : Research Dissemination for Community Development International Journal of Engineering, Technology and Natural Sciences (IJETS) Jurnal Berdaya Mandiri Jurnal Sains dan Edukasi Sains Indonesian Journal of Applied Research (IJAR) Indobiosains Bioscientist : Jurnal Ilmiah Biologi Jurnal Pengabdian Masyarakat Indonesia International Journal of Research in Community Services Proceeding Biology Education Conference Aptekmas : Jurnal Pengabdian Kepada Masyarakat International Journal on Education Insight Journal of Biotechnology and Natural Science Filogeni: Jurnal Mahasiswa Biologi Jurnal Pengabdian Masyarakat Bangsa DIMASLIA Jurnal Abdimas NJCOM: Community Service Journal Biodiversitas Journal of Biological Diversity
Oktira Roka Aji
Program Studi Biologi, Fakultas Sains Dan Teknologi Terapan, Universitas Ahmad Dahlan
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Transportation Veterinary Other

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The effectiveness of salak pondoh peel ecoenzyme spray gel (Salacca edulis Reinw.) in pre-clinical evaluation of grade II burn healing Kinasti Dwi Lestari; Aisyah Aulia Nugraha; Vibula Rosa Arumbina; Umu Kamilah; Rara Asti Azizah; Haris Setiawan; Oktira Roka Aji
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 2 (2025): December
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i2.4277

Abstract

Burns are a serious health problem and require proper treatment for healing. Salak pondoh (Salacca edulis Reinw.) peel is known to contain high flavonoid compounds that have potential as antioxidants and antimicrobials. This study aims to determine the antibacterial activity of salak pondoh peel ecoenzyme and evaluate the effectiveness of its spray gel in healing burn wounds in rats.  The research began with the preparation of eco-enzyme, flavonoid test using tube test and spectrophotometry, antibacterial test using disc diffusion method against Staphylococcus aureus and Pseudomonas aeruginosa, formulation and spray gel test, antioxidant test using DPPH method, and in vivo test to observe the percentage of second degree burn wound healing in male Wistar rats. The in vivo experiment involved 28 rats divided into five treatment groups: Control (K), Positive Control (KP), Negative Control (KN), 15% spray gel (P1), and 20% spray gel (P2). Data from in vitro and in vivo tests were analyzed using One-Way ANOVA (Analysis of Variance) with a significance level >0.05, followed by Tukey's Posthoc test.  The results showed that 15% salak pondoh peel eco-enzyme showed strong antibacterial activity with an inhibition zone of 11.83 ± 0.76 against the growth of S. aureus and 10.83 ± 0.28 in the inhibition zone of P. aeruginosa.  The 20% concentration (P2) showed the highest effectiveness in accelerating burn wound healing in test rats, with a healing rate of 99% (p < 0.05) on day 18.  In conclusion, salak pondoh peel eco-enzyme showed antibacterial activity, and the eco-enzyme spray gel effectively accelerated burn wound healing. 
In Vitro Pancreatic Lipase Inhibitor Activity of Mangifera foetida Leaves Extract Oktira Roka Aji; Riris Asyhar Hudaya; Diah Asta Putri
Jurnal Biodjati Vol 6 No 1 (2021): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i1.10646

Abstract

Obesity is a condition where there is excessive fat in the body. Weight loss can be accomplished by inhibiting fat absorption through the inhibition of pancreatic lipase activity, a key enzyme in fat metabolism. Natural products from plants contain various phytochemical compounds that can act as pancreatic lipase inhibitors. This research was conducted to determine the lipase inhibitor activity of the methanol extract of Mangifera foetida leaves. Mangifera foetida leaves were extracted and then the total phenolic and flavonoid levels were determined. The extract was tested for its ability to inhibit pancreatic lipase in vitro. The inhibitory activities of the extract and Orlistat were measured using p-nitrophenyl palmitate as a substrate at concentrations of 31.25, 62.5, 125, 250, and 500 µg/mL. The results showed that the extracts of young leaves (YL) and mature leaves (ML) contained phenol and flavonoid compounds. The IC50 values of young leaf extract (YL) and mature leaves (ML) were 45.22 and 35.50 µg/ml, respectively. In conclusion, the Mangifera foetida leaf extract can be promoted as a good source of anti-obesity compounds
Comparison of Quality and Ethanol Content of Liquid and Powder Kombucha from Jasmine Tea (Jasminum sambac L.) Nisa Ihsani; Anisa Nurul Huda; Arifa Nadya Nur Afifah; Tiwi Sartika; Linda Pertiwi; Maelita Ramdani Moeis; Muhammad Farid Maksum; Aila Gema Safitri; Oktira Roka Aji
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.47357

Abstract

The fermentation process of kombucha has become a focus of halal product research due to its ethanol content. Processing kombucha into powder form can be an alternative to address the ethanol content in liquid kombucha, as it has undergone a drying process, making it a potential halal-certified functional drink. The objective of this study was to compare the quality of liquid and powdered kombucha. The research was conducted from March to September 2024 at the Biotechnology Laboratory, Muhammadiyah University of Bandung. The method used was the Complete Randomized Design (CRD) method. The parameters analyzed included ethanol content, total BAA (Bacterial Acetic Acid), pH, organic acid content, and sugar content. In the production of powdered kombucha, optimization of maltodextrin and gum arabic usage was also conducted to achieve the best solubility and taste. The best powdered kombucha was obtained with a maltodextrin-to-gum arabic ratio of 3:2. In the research, liquid kombucha contained ethanol at an average of 0.09%. In comparison, no ethanol was detected in the powdered kombucha (0%). Total BAA in powder decreased after the drying process (p<0.05). The pH value of kombucha in liquid form (4.4) also differed significantly from powdered (3.2) (p<0.05). However, the levels of organic acids and sugars in liquid and powdered kombucha did not differ significantly (p>0.05). The sugar content in liquid and powdered kombucha was lower than that in kombucha in general.
Pemanfaatan Keanekaragaman Tumbuhan Untuk Pembuatan Batik Ecoprint di Desa Sidomulyo, Yogyakarta Oktira Roka Aji; Ambar Pratiwi; Nursyiva Irsalinda
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 20, No 1 (2023): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Many villages face challenges in optimizing their local potential. Sidomulyo Village, Bambanglipuro, Bantul, is one of the villages with abundant natural resources, including diverse plant species that have not been fully utilized. To address this, a community engagement program has been designed to provide training on the utilization of plant diversity in Sidomulyo Village for creating ecoprint batik fabric. The method employed in this community engagement program involves training in ecoprint creation using natural dyes and various local plant leaves. Throughout the program's implementation, the activities have been successful and have yielded positive outcomes. Participants' knowledge about ecoprint has increased significantly, from 20% to 100%. The enthusiasm of the participants in participating in the ecoprint training has been remarkable. Beyond the knowledge enhancement, many participants have shown an interest in further developing ecoprint variations, and some are even keen on establishing ecoprint businesses. Through this program, it is hoped to stimulate the growth of the creative economy by harnessing the natural potential within the surroundings of Sidomulyo Village. All of these efforts contribute to the improvement of the well-being and empowerment of the local community.
Bacterial diversity and antibacterial activity of fermented buni fruit (Antidesma bunius) kombucha OKTIRA ROKA AJI; TRIANIK WIDYANINGRUM; PUTRI NABELA
Biodiversitas Journal of Biological Diversity Vol. 26 No. 8 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260811

Abstract

Abstract. Aji OR, Widyaningrum T, Nabela P. 2025. Bacterial diversity and antibacterial activity of fermented buni fruit (Antidesma bunius) kombucha. Biodiversitas 26: 3781-3790. Kombucha is a fruit-based fermented beverage with a number of health benefits. Fruit-based substrates can enhance their nutritional value and microbial diversity. This study aimed to characterise the bacterial diversity and antibacterial activity of kombucha produced from buni (Antidesma bunius) fruits. Buni kombucha was fermented statically for 7, 14, and 21 days, and bacterial diversity was analysed by 16S rRNA (V3-V4) amplicon sequencing using the SILVA database. Antibacterial activity was evaluated against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtilis using the agar well diffusion method. Metagenomic analysis revealed Komagataeibacter (~80%) as the dominant genus, while low-abundance taxa such as Lactobacillus, Bacillus, and Bifidobacterium were detected; trace gut-associated genera (e.g., Bacteroides, Prevotella) likely originated from the pectin-rich substrate. Antibacterial activity increased significantly with fermentation time (p&lt;0.05), with the strongest inhibition after 21 days: 13.83 ± 0.29?mm (S. aureus), 13.70 ± 0.61?mm (P. aeruginosa), 12.23 ± 0.25?mm (B. subtilis), and 10.77 ± 0.70?mm (E. coli). These results demonstrate that buni kombucha harbours a diverse microbiota and exhibits broad-spectrum antibacterial activity, supporting its potential as a postbiotic-rich functional beverage derived from local tropical fruits.
Antagonistic and Antifungal Activities of Endophytic Fungi Isolated from Fragaria x Ananassa Against Colletotrichum Acutatum Oktira Roka Aji; Alfiatul Laila
Jurnal Internasional Teknik, Teknologi dan Ilmu Pengetahuan Alam Vol 7 No 1 (2025): International Journal of Engineering, Technology and Natural Sciences
Publisher : Universitas Teknologi Yogyakarta, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46923/ijets.v7i1.393

Abstract

Fragaria x ananassa or strawberry has significant economic value worldwide. However, its cultivation faces various challenges. One example was fungal diseases, such as anthracnose, caused by Colletotrichum acutatum. Disease management generally relies heavily on synthetic fungicides, raising concerns about environmental sustainability and human health risks. Therefore, more environmentally friendly alternatives are needed, such as the use of biological agents. This study aims to assess the potential of endophytic fungi from strawberry plants as natural biocontrol agents against Colletotrichum acutatum. The study began with the isolation of endophytic fungi from strawberry tissues and their characterization morphologically. The antagonistic activity of endophytic fungi against colletotrichum acutatum was evaluated using three methods: a dual culture assay to assess direct inhibition, a cell-free filtrate assay to analyze the effect of fungal metabolites, and a volatile organic compound (VOC) assay to inhibit the growth of pathogenic pathogens. In this study, 20 fungal isolates were successfully isolated. A total of 15 showed antagonism against Colletotrichum acutatum. Isolate A1 showed potent inhibition in the dual culture test by effectively antagonizing Colletotrichum acutatum. Isolate D1 produced extracellular compounds that reduced the growth of Colletotrichum acutatum by 52.28% ± 3.21% in the cell-free filtrate assay. At the same time, isolate D3 proved most effective in the VOC assay, suppressing the growth of pathogenic colonies by 71.35% ± 3.37%. Thus, endophytic fungi, especially isolates A1, D1, and D3, have the potential to serve as an environmentally friendly alternative to combat anthracnose disease on strawberries.
Penerapan Keselamatan dan Kesehatan Kerja pada Lahan Miring di Sanggar Tani Kalurahan Serut Gunung Kidulul Subhan Zul Ardi; Yudi Ari Adi; Umi Salamah; Arif Rahman; Oktira Roka Aji
NJCOM: Community Service Journal Vol. 2 No. 2 (2026): JULY
Publisher : RAM PUBLISHER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5281/zenodo.20095148

Abstract

Penerapan K3 selama ini lebih terfokus pada sektor formal yang memiliki struktur organisasi dan belum terfokus ke sektor informal. Oleh karena itu, penting untuk melakukan kajian mengenai penerapan K3 di sektor pertanian informal sebagai upaya dalam meningkatkan kesadaran akan keselamatan dan kesehatan. Desa Serut adalah salah satu desa yang terletak di Kecamatan Gedangsari, Kabupaten Gunung kidul dengan mayoritas bekerja sebagai buruh tani. Tujuan dari pengabdian adalah implementasi K3 pada pembukaan lahan pisang oleh sanggar tani muda pemerintah kalurahan serut. Solusi yang ditawarkan adalah para petani di desa serut memahami dan mengimplementasikan keselamatan ketika bekerja termasuk kondisi di lahan. Mitra dalam kegiatan PKM adalah Sanggar tani muda, dan pemerintah kalurahan Serut. Hasil yang telah dicapai adalah peningkatan pengetahuan pada para petani di sanggar tani muda.
Co-Authors Abdul Aziz Adelyanty Pangestuty Wicaksono Adz-Dzikir, Fatihatuljannah Aila Gema Safitri Aisyah Aulia Nugraha Aisyah, Iis Nisya Nur Al Farisi, Irsyad Alfiatul Laila Alya Firli Refanza Putri Ambar Pratiwi Andika Bayu Chandra Aninda Kumala Sari Anisa Nurul Huda Ardi, Subhan Zul Arif Rahman Arifa Nadya Nur Afifah ARUM PATRIATI Astuti, Sutan Nur Chamida Tri Azizah, Mar'atul Cahya Ningsih, Ayu Chlara Jelviora Cucu Cahyanti Diah Asta Putri Diah Asta Putri Diah Asta Putri Diah Asta Putri DYSHELLY NURKARTIKA PASCAPURNAMA EDY GIRI-RACHMAN PUTRA Efrida Siti Alifia Ega Asnatasia Maharani Ernawati A. Giri Rachman Falih, Afifah Nurul FENRYCO PRATAMA Fitria, Karomah Indah Handayani, Erisa Budi Haris Setiawan Hartanto, Dody Haryadi, Bagus Hasna Chaerani Zakkiyah Hidayah, Hidayah Ihsanawati Ihsanawati Ihsani, Nisa Ika Maryani Ilma, Nur Malika Imelda Pangaribuan Imelda Pangaribuan Inggita Utami Irsalinda, Nursyiva Irvanita, Deva Anggun Jannati Aulah Jinan, Rifa Fatma Karomah Indah Fitria Kinasti Dwi Lestari Kurnia, Dhiffa Trisna Larasati Haliimah Roosyidah Lestari, Iva Dita Linda Pertiwi MAELITA RAMDANI MOEIS Maelita Ramdani Moeis MAELITA RAMDHANI MOEIS Maliza, Rita Mardiah, Irma Merlia Rahma Tari Muhammad Farid Maksum Nabela Dwi Wahyu Nurvi Fibriyanti Naresh Wari Mega Vianingtyas Nisa Ihsani Nisa Ihsani Nofa Nur Fiani Nora Bastiani Nugraha, Farhan Satya Nur Azizah Nur Azizah Nuraisyah Binte Zainal Nursyiva Irsalinda Nursyiva Irsalinda Nurul Suwartiningsih Pinasti, Vanya Aurel Pratiwi, Ambar Pratiwi, Ambar Puguh Wahyu Prasetyo, Puguh Purwanti Pratiwi Purbosari Puspitarini, Sofia PUTRI NABELA Putri , Diah Asta Putri Nabela Putri, Anas Tasya Putri, Aulia Ramanda Putri, Diah Asta Putri, Nida’a Fauziyyah Rara Asti Azizah Rinaldi Rizal Putra Riris Asyhar Hudaya Rohmantin, Dita Rossa Puteri Baharie, Sri Safitri, Salsabila Salimah, Azizah Zahratus Santrianda, Aldhiani Silfi Pratiwi Siti Nurhasanah Sri Wahyuni Sri Wahyuni Subhan Zul Ardi Sumbudi, Milliniawati Suwartiningsih, Nurul Suwartiningsih, Nurul Tiwi Sartika Tri Puspita Sari TRIANIK WIDYANINGRUM Trianik Widyaningrum Tufahati, Nadhira Umi Salamah Umi Salamah Umu Kamilah Vibula Rosa Arumbina Wachid Eko Purwanto Wulandari, Endah Retno Yudi Ari Adi Yudi Ari Adi, Yudi Ari Yuliani, Ririn Kholifatu Zahra, Safa Auli Zakkiyah, Hasna Chaerani Zul Ardi, Subhan