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All Journal HAYATI Journal of Biosciences Microbiology Indonesia Health Science Journal of Indonesia Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Prosiding Seminar Biologi Jurnal Sumberdaya Hayati Indonesian Journal of Cancer The Indonesian Biomedical Journal Molecular and Cellular Biomedical Sciences (MCBS) Acta Biochimica Indonesiana Jurnal Ilmu Kefarmasian Indonesia The New Ropanasuri Journal of Surgery Muhammadiyah Journal of Geriatric Indonesian Journal of Cancer eJournal Kedokteran Indonesia Makara Journal of Science Nanggroe: Journal Of Scholarly Service Molekul: Jurnal Ilmiah Kimia Proceedings Book of International Conference and Exhibition on The Indonesian Medical Education and Research Institute Biomedika Jurnal Info Kesehatan

Mohamad Sadikin

Department Of Biochemistry And Molecular Biology, Center Of Hypoxia And Oxidative Stress Studies, Faculty Of Medicine, Universitas Indonesia, Jakarta

Author-ID : 367731

Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Cytoglobin expression in rat kidney during exposure to systemic chronic hypoxia Ika Superti Daruningrum; Ani Retno Prijanti; Ninik Mudjihartini; Mohamad Sadikin; Sri Widia A Jusman
Acta Biochimica Indonesiana Vol. 3 No. 1 (2020): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v3i1.55

Background: The kidneys in physiological conditions are always in a state of relative hypoxia. Cytoglobin (Cygb) is the newest globin protein found of the globin family. One of the functions of Cygb is in oxygen supply. Cygb expression is found to increase in hypoxic conditions, which are thought to be an adaptation response to hypoxia. Objective: This study aimed to analyze the expression of Cygb in rat kidneys which were exposed to chronic systemic hypoxia. Methods: Twenty five male Sprague-Dawley rats, weighing 150-200 g were used in this experiment. Rats were divided into 5 groups: The control group was exposed to normoxia; the hypoxia groups (10% oxygen / 90% nitrogen) for 1 day; 3 days; 7 days and 14 days. After treatment, rats were sacrificed and their kidneys were taken. Cygb mRNA expression was measured by qRT-PCR, while Cygb protein expression was measured by the ELISA method. Results: The expressions of Cygb mRNA and protein were found to be highest on day 3 of hypoxia and was correlated very strongly and significantly (r2 = 0.96; p <0.05). Conclusion: The highest expression of Cygb on day 3 of chronic systemic hypoxia exposure is suggested as an attempt to restore oxygen supply to the kidneys.

Peningkatan Kadar Imunosupresan IDO (Indoleamin 2,3-dioksigenase) pada Supernatan Kultur Sel Punca Mesenkim yang Distimulasi dengan Agregat Imunoglobulin G DIAN RATIH LAKSMITAWATI; JEANNE ADIWINATA PAWITAN; MOHAMAD SADIKIN; CAROLINE TAN SARDJONO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 12 No 1 (2014): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (480.939 KB)

Mesenchymal stem cells have been known to have the nature of supressing immune response. This cell have been proven to reduce the symptoms of organ transplants rejection. Clinically proofing is also being performed on an autoimmune disease caused by the complex immune such as systemic lupus erythematosus (SLE). This study aimed to know aggregated IgG-stimulating effects of mesenchymal stem cells in relation to IDO secretion, an immunosuppressant agent. Mesenchymal stem cells was isolated from liposuction waste and characterized based on its surface molecule accrording to ISCT criterias. The confirmed cell culture was used to the next culture by the addition of heat aggragated immunoglobulins gamma (HAGG) for six days. IDO secreted on supernatant culture was collected and assayed by elisa method at 450 nm. The results showed that there was elevated levels of IDO (16.73-49.5%) on culture with HAGG stimulation than without stimulation. This study could be a part of the basic

Isolasi dan Karakterisasi Protease Serupa Tripsin (PST) dari Lactobacillus plantarum FNCC 0270 TRISMILAH TRISMILAH; WAHONO SUMARYONO; AMARILA MALIK; MOHAMAD SADIKIN
JURNAL ILMU KEFARMASIAN INDONESIA Vol 12 No 1 (2014): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (665.7 KB)

Trypsin is an enzyme which is important for research as well as for the pharmaceutical industry, medicine and health, which is especially developed as a raw material for digestive enzymes. This study aimed to characterize PST from Lactobacillus plantarum FNCC 0270 that have not been studied as a producer of trypsin. Fermentation for producing PST by L . plantarum FNCC 0270 was performed in the LKB fermentor with working volume of 3.5 liters, at temperature of 37 oC, pH value of 8, agitation rate of 77 rpm and aeration of 0.5 vvm. The media composition were 3.64% baker’s yeast, 1.21% glucose, 0.13% skim milk. The fementation resulted in enzyme activities of 0.00129 U/mL and protein content of 0.49 mg/mL. Purification of the PST conducted by ultrafiltration Hollow Fiber Catridge 5 kD, presipitated with ammonium sulfate saturated (30-70%), 30 kD membrane polyesthersulfone, ion exchange column chromatography Q-XL and HiTrap affinity column chromatography. All of system increased purity of the crude PST. SDS-PAGE of PST indicated the molecular weights of 47.35; 38.42;21.40 and 12.96 kD. The PST was stable at pH value of 8 and temperature range of 25-35 oC. It is proved that the same half-life was 693.2 minutes. Km and Vmax values of the PST were 0.231 mM and 0.001 U/mL, respectively using N-α-benzoyl-DL-arginine-p-nitroanilide as the substrate. PST was inhibited by EDTA, Ca2+, Zn2+, Mg2+, Mn2+ and specific substrates. Based on the result of characterization of PST from L. plantarum FNCC 0270 can be concluded that the trypsin–like protease (PST) was trypsin.

Ekspresi Relatif mRNA Hypoxia Inducible Factor-1? pada Sel Glioma Penderita Novi Silvia Hardiany; Mohamad Sadikin; Septelia Inawati Wanandi
Indonesian Journal of Cancer Vol 7, No 1 (2013): Jan - Mar 2013
Publisher : National Cancer Center - Dharmais Cancer Hospital

Glioma merupakan tumor otak primer yang cukup sering ditemukan di Indonesia di antara keganasan otak lainnya. Penderita glioma mempunyai angka harapan hidup yang rendah dikarenakan seringnya mengalami resistansi terapi. Hipoksia merupakan salah satu faktor yang mempengaruhi respons terapi radiasi. Penelitian ini menganalisis ekpresi mRNA HIF-1?, sebagai petanda jaringan hipoksia, pada glioma derajat rendah dibandingkan dengan glioma derajat tinggi. Sampel terdiri dari 15 jaringan glioma derajat rendah, 6 jaringan glioma derajat tinggi, dan 21 sel lekosit dari penderita glioma sebagai kontrol sel normal. Ekspresi relatif mRNA HIF-1? ditentukan secara kuantitatif dengan menggunakan Real Time RT-PCR. Hasil penelitian menunjukkan bahwa ekspresi mRNA HIF-1? pada glioma derajat tinggi lebih tinggi dibandingkan dengan glioma derajat rendah, namun tidak bermakna secara statistik. Hal yang menarik yaitu ditemukan peningkatan ekspresi mRNA HIF-1? dengan nilai yang bervariasi pada 73 % sampel glioma derajat rendah dibandingkan dengan sel lekosit sebagai kontrol sel normal. Begitu juga pada glioma derajat tinggi ditemukan peningkatan mRNA HIF-1? dengan nilai yang bervariasi pada 100% sampel. Dengan demikian, dapat disimpulkan bahwa terdapat variasi derajat hipoksia pada glioma derajat rendah dan derajat tinggi yang mungkin mempengaruhi keberhasilan terapi radiasi.Kata kunci: Glioma, mRNA HIF-1?, hipoksia

Mitochondrial Adaptations of Obese Adult Rat Visceral Adipocytes which Differ in Nutritional Status during Childhood Lailan Safina Nasution; Ahmad Aulia; Sri Widia A. Jusman; Mohamad Sadikin
eJournal Kedokteran Indonesia Vol 9, No. 3 - Desember 2021
Publisher : Faculty of Medicine Universitas Indonesia

Mitochondria are highly dynamic organelles that can adapt to different environmental stimuli. This study aims to analyze differences in the function and biogenesis of mitochondria and anaerobic glycolysis activity between obese adult rats that were undernourished in childhood (LCD28) and those which were normal (SD28) or already fat (HFD28). Sprague-dawley rats were assigned groups and given diet treatments from December 2017 until August 2018 in the Laboratory Animal Management Unit, Faculty of Veterinary Medicine, Bogor Agricultural Institute. To observe mitochondrial function and biogenesis, MnSOD activity and PGC1α levels were analyzed using ELISA. To investigate the existence of anaerobic glycolysis, LDH activity was analyzed using a spectrophotometry method. The results showed that MnSOD activity and PGC1α levels of LCD28 increased compared to the other groups, signifying higher mitochondrial function and biogenesis. Meanwhile, no significant difference in LDH activity was found, signifying similar levels of anaerobic glycolysis.

Optimization of Trypsin-like Protease Production by Lactobacillus plantarum FNCC 0270 using Response Surface Methodology Trismilah,; Nurhasanah, Astutiati; Sumaryono, Wahono; Malik, Amarila; Sadikin, Mohamad
Makara Journal of Science Vol. 19, No. 2
Publisher : UI Scholars Hub

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The purpose of this study was to get optimum medium composition and agitation to trypsin-like protease production by Lactobacillus plantarum FNCC 0270. The medium composition and agitation for enzyme production was optimized using Central Composite Design and Response Surface Method with Design Expert software version 7.1.5 Fermentation was carried out in erlenmeyer flasks at initial pH 8, 37 °C, using an incubator shaker at 87.5 rpm. The best results showed an enzyme activity of 1.0 mU/mL, a protein level of 0.557 mg/mL, and desirability value of 0.740. Numerical optimization was performed to approach the ideal state of the fermentation or the desirability value of 1. The medium composition containing of 3.64% baker's yeast, 1.21% glucose, and 0.13% skim milk was used for the fermentation. The enzyme activity of 1.51 mU/mL and protein level of 0.205 mg/mL can be achieved. After numerical optimization, the fermentation process was verified in erlenmeyer flasks with incubator shaking at 77 rpm, initial pH 8, 37 °C, and 15 h fermentation. The verification results showed that the enzyme activity of 1.273 ± 0.227 mU/mL and protein level of 0.248 ± 0.012 mg/mL.

Oxidative Stress, Hypoxia-Inducible Factor-1α, and Nuclear Factor-Erythroid 2-Related Factor 2 in the Hearts of Rats Exposed to Intermittent Hypobaric Hypoxia Wardaya Wardaya; Wawan Mulyawan; Sri Widia A Jusman; Mohamad Sadikin
HAYATI Journal of Biosciences Vol. 31 No. 1 (2024): January 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.1.39-47

Hypobaric hypoxia is situation that might occur to helicopter pilots in Indonesia who must fly at an altitude of more than 3,048 m such as in Papua. It can be dangerous because hypoxic condition can affect person's performance. So far, the heart is known as an aerobic organ and very sensitive to hypoxic conditions. Hitherto, the effects of hypobaric hypoxia exposure on biomolecular aspects of the heart are still unclear. Therefore, this study assessed cardiac response in rats exposed to intermittent hypobaric hypoxia (IHH) (equivalent to 3,048 meters/10,000 feet). Sprague-Dawley rats were divided into six groups: control; acute hypobaric hypoxia (AHH); and IHH, for 7; 14; 21; and 28 days. We measured super oxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities, reduced glutathione (GSH), malondialdehyde (MDA), cytoglobin, myoglobin, HIF-1α, and Nrf2 level as our parameters. Activities of SOD, CAT, GPx and GSH increased while the levels of MDA, cytoglobin, myoglobin, HIF-1α, and Nrf2 decreased in all IHH groups compared with the AHH group. A biphasic pattern was observed as IHH sessions increased from 14 to 21 or 28. Where the IHH treatment for more than 14 sessions caused a decrease in endogenous antioxidants, but the response to hypoxia and oxidative stress increased. Our findings presented the molecular alterations of cardiac rats exposed to intermittent hypobaric hypoxia.

Karakteristik dan Peran Protein Ikat Folat (PIF) Subandrate; Dwirini Retno Gunarti; Mohamad Sadikin
Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Vol. 3 No. 1 (2016): Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universi
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/jkk.v3i1.48

Asam folat merupakan kelompok vitamin B. Asam folat terikat dengan suatu protein yang disebut protein ikat folat (PIF). PIF merupakan suatu glikoprotein dengan 222 residu asam amino dan berat molekul sekitar 31000 Dalton. Struktur tiga dimensi PIF dipertahankan oleh enam sampai delapan jembatan disulfida. PIF memiliki kemampuan mengikat asam folat dengan perbandingan ikatan 1:1 mol. Situs aktif PIF mengandung gugus imidazol atau gugus sulfhidril. Kofaktor ion kalsium tidak dibutuhkan PIF dalam mengikat asam folat. Di dalam tubuh, PIF berperan menghimpun, mengangkut, dan membantu penyimpanan, penggunaan serta pendistrubusian asam folat ke jaringan. PIF juga berperan mengurangi ekskresi asam folat di ginjal dan meningkatkan penyerapan asam folat di intestinum. Dalam bidang kedokteran PIF dapat dimanfaatkan untuk mengukur kadar asam folat serum dengan metode analog ELISA.

The Role of Hypoxia-inducible Factor in Mycobacterium tuberculosis-infected Macrophages Fitriana, Nina; Iswanti, Febriana Catur; Sadikin, Mohamad
Molecular and Cellular Biomedical Sciences Vol 8, No 1 (2024)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v8i1.405

Tuberculosis is caused by Mycobacterium tuberculosis infection. During M. tuberculosis infection, there is a decrease in the partial pressure of oxygen in the granuloma microenvironment, which causes the hypoxia-inducible factor (HIF) to become stable. HIF functions as a transcription factor that regulates the expression of genes crucial for metabolic adaptation in hypoxic conditions. Recent research suggests that HIF plays a vital role in infectious and inflammatory conditions. Several studies have demonstrated that HIF signaling can enhance macrophages antimicrobial activity and bactericidal effect against M. tuberculosis, such as increasing macrophage autophagy, enhancing the effects of rifampicin, inhibiting p38 MAPK signaling, enhancing the regulation of effector antimicrobial pathways mediated by human β defensin 2 (hBD2) and vitamin D receptor (VDR), redirecting energy metabolism to glycolysis, and producing various cytokines. All these responses ultimately result in the inhibition of intracellular M. tuberculosis growth. HIF has therapeutic implications, potentially being a new candidate for host-directed therapy as a complement to existing antituberculosis drugs. Understanding the role of HIF in macrophages during M. tuberculosis infection and comprehending the host-pathogen relationship with M. tuberculosis is advantageous for developing future therapies.Keywords: Mycobacterium tuberculosis, macrophages, hypoxia-inducible factor

Isolation and Purification of Thiamine Binding Protein from Mung Bean GUNARTI, DWIRINI RETNO; RAHMI, HANIFAH; SADIKIN, MOHAMAD
HAYATI Journal of Biosciences Vol. 20 No. 1 (2013): March 2013
Publisher : Bogor Agricultural University, Indonesia

Thiamine has fundamental role in energy metabolism. The organs mostly sensitive to the lack of thiamine levels in the body are the nervous system and the heart. Thiamine deficiency causes symptoms of polyneuritis and cardiovascular diseases. Because of its importance in the metabolism of carbohydrates, we need to measure the levels of thiamine in the body fluids by using an easy and inexpensive way without compromising the sensitivity and selectivity. An option to it is thiamine measurement based on the principle of which is analogous to ELISA, in which a thiamine binding protein (TBP) act by replacing antibodies. The presence of TBP in several seeds have been reported by previous researchers, but the presence of TBP in mung beans has not been studied. This study was aimed to isolate and purify TBP from mung bean. The protein was isolated from mung bean through salting out by ammonium sulphate of 40, 70, and 90% (w/v). TBP has a negative charge as shown by cellulose acetate electrophoresis. The result obtained after salting out by ammonium sulphate was further purified bymeans of DEAE-cellulose chromatography and affinity chromatography. In precipitation of 90% of salting out method, one peak protein was obtained by using affinity chromatography. The protein was analyzed by SDS PAGE electrophoresis. The result of SDS PAGE electrophoresis showed that TBP has a molecular weight of 72.63 kDa.

Co-Authors . RUSDI . Rusdi Ahmad A. Jusuf Ahmad Aulia Ahmad Aulia Jusuf Ahmad R. Utomo Aldri Frinaldi Amarila Malik Andi N.K. Syarifin, Andi N.K. ANDREW ROBERT COSSINS Angelina Stevany Regina Masengi Ani R. Prijanti Ani Retno Prijanti Ardiana Kusumaningrum Arfat Lusinanto Arief Budi Witarto Aryenti . Asri Rasad Astutiati Nurhasanah ASTUTIATI NURHASANAH Bethy S. Hernowo Caroline T. Sardjono CAROLINE TAN SARDJONO Cicia Firakania, Cicia DARYL ROBERT WILLIAMS Dewi Sukmawati Diah Handayani Diah Iskandriati Dian R. Laksmitawati Dian Ratih Laksmitawati Dwirini Retno Gunarti Dwirini Retno Gunarti Ekawati, Maria Evy Ayu Arida Fanny Septiani Farhan Febriana C. Iswanti Febriana Catur Iswanti Franciscus D. Suyatna Frans Ferdinal Frans Ferdinal HANIFAH RAHMI Hans -Joachim Freisleben Hans Joachim Freisleben HANS-JOACHIM FREISLEBEN Heri Wibowo Hermansyah Hermansyah Ichramsyah A. Rahman Ika Superti Daruningrum Indra G. Mansur Indra Gusti Mansur Irawan Satriotomo Jeanne A. Pawitan Jeanne Adiwinata Pawitan Juniarti . KURNIA NUZTIR MANTOLINI Lailan Safina Nasution Lindi G Haritsyah M, Mentari Mohamad Saekhu Muchlis Ramli Muhamad Arif Budiman Muhamad Yusup Muhammad Faris Indratmo Nani Dharmasetiawani Nani Sari Murni Ni Ken Ritchie Nina Fitriana, Nina Ninik Mudjihartini Noorwati Sutandyo Novi S. Hardiany Novi Silvia Hardiany Novianti, Titta Nurhadi Ibrahim Nurhadi Ibrahim Nurjati C. Siregar Oentoeng Soeradi Pamungkas, Joko Panjaitan, Paris Praditi, Citra Radiana D. Antarianto Rahmawati Ridwan Rahmawati Ridwan Ranti Dwi Astriani Reni Paramita Resda Akhra Syahrani Ria Syafitri Evi Gantini Rini Puspitaningrum RONDANG ROEMIATI SOEGIANTO RONDANG ROEMIATI SOEGIANTO RONDANG ROEMIATI SOEGIANTO Rostika Flora Sadikin, Abdul Halim Saleh, Mgs. M. Irsan Samsuridjal Djauzi Sari, Dewi H. SARI, MIKE PERMATA Sarmoko Sarsanti, Pungguri Ayu Nega Sekar Arumsari Septelia I. Wanandi SEPTELIA INAWATI WANANDI Siregar, Nurjati Siti Rahmawati Achyat Sitorus, Novandrik Yeriko Siufui Hendrawan Sri S. Ningsih, Sri S. Sri W.A. Jusman Sri Widia A.Jusman Subandrate Suryani, N. Lilis Syarifah Dewi Syazili Mustofa Tiara Mayang Pratiwi Lio Tiwuk Susantiningsih Tomohiko Yamazaki Trismilah Margono Trismilah Trismilah TRISMILAH TRISMILAH Trismilah, Uly A. Nikmah, Uly A. Vetnizah Juniantito Wahono Sumaryono Wardaya Wardaya Wawan Mulyawan Wawan Mulyawan Wawan Mulyawan Wawan Mulyawan Wawan Mulyawan Wawan Mulyawan Wiwoho, Yudi Yuwono Yalista Fatia Nadia Yefta Moenadjat Yudianita Kesuma, Yudianita Yuhernita . Zen Hafy

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