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All Journal HAYATI Journal of Biosciences Microbiology Indonesia Health Science Journal of Indonesia Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Prosiding Seminar Biologi Jurnal Sumberdaya Hayati Indonesian Journal of Cancer The Indonesian Biomedical Journal Molecular and Cellular Biomedical Sciences (MCBS) Acta Biochimica Indonesiana Jurnal Ilmu Kefarmasian Indonesia The New Ropanasuri Journal of Surgery Muhammadiyah Journal of Geriatric Indonesian Journal of Cancer eJournal Kedokteran Indonesia Makara Journal of Science Nanggroe: Journal Of Scholarly Service Molekul: Jurnal Ilmiah Kimia Proceedings Book of International Conference and Exhibition on The Indonesian Medical Education and Research Institute Biomedika Jurnal Info Kesehatan

Mohamad Sadikin

Department Of Biochemistry And Molecular Biology, Center Of Hypoxia And Oxidative Stress Studies, Faculty Of Medicine, Universitas Indonesia, Jakarta

Author-ID : 367731

Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Articles

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Ekspresi Relatif mRNA Hypoxia Inducible Factor-1? pada Sel Glioma Penderita Hardiany, Novi Silvia; Sadikin, Mohamad; Wanandi, Septelia Inawati
Indonesian Journal of Cancer Vol 7, No 1 (2013): Jan - Mar 2013
Publisher : "Dharmais" Cancer Center Hospital

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (530.441 KB)

Glioma merupakan tumor otak primer yang cukup sering ditemukan di Indonesia di antara keganasan otak lainnya. Penderita glioma mempunyai angka harapan hidup yang rendah dikarenakan seringnya mengalami resistansi terapi. Hipoksia merupakan salah satu faktor yang mempengaruhi respons terapi radiasi. Penelitian ini menganalisis ekpresi mRNA HIF-1?, sebagai petanda jaringan hipoksia, pada glioma derajat rendah dibandingkan dengan glioma derajat tinggi. Sampel terdiri dari 15 jaringan glioma derajat rendah, 6 jaringan glioma derajat tinggi, dan 21 sel lekosit dari penderita glioma sebagai kontrol sel normal. Ekspresi relatif mRNA HIF-1? ditentukan secara kuantitatif dengan menggunakan Real Time RT-PCR. Hasil penelitian menunjukkan bahwa ekspresi mRNA HIF-1? pada glioma derajat tinggi lebih tinggi dibandingkan dengan glioma derajat rendah, namun tidak bermakna secara statistik. Hal yang menarik yaitu ditemukan peningkatan ekspresi mRNA HIF-1? dengan nilai yang bervariasi pada 73 % sampel glioma derajat rendah dibandingkan dengan sel lekosit sebagai kontrol sel normal. Begitu juga pada glioma derajat tinggi ditemukan peningkatan mRNA HIF-1? dengan nilai yang bervariasi pada 100% sampel. Dengan demikian, dapat disimpulkan bahwa terdapat variasi derajat hipoksia pada glioma derajat rendah dan derajat tinggi yang mungkin mempengaruhi keberhasilan terapi radiasi.Kata kunci: Glioma, mRNA HIF-1?, hipoksia

Qualitative Study on Endothelial Cell–to–cell–junction Disassembly in Severe Burn Injury Moenadjat, Yefta; Siregar, Nurjati C.; Wanandi, Septelia I.; Sadikin, Mohamad
The New Ropanasuri Journal of Surgery Vol. 2, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Introduction. Endothelial gap in severe burn injury remain a mystery. Capillary leaks possess its own characteristics, which is found in burned– and non–burned area. The gaps remain up to 10 post burned days or more. This is somehow representing the feature of systemic capillary leaks syndrome at the first date. VE–cadherin of adherens endothelial junction molecules known to be temporarily disassembled following thermal exposure, but there’s a question about reversibility. Question is also addressed to occludin of the tight junction molecules. We run a study to investigate these junction molecules. Method. We run an investigation to find out both molecules qualitatively descriptive on 30 burn patients enrolled, consist of 20 severe– and 10 of non–severe burn. Samples of moderate size vein taken from burned– and non–burned area were subjected to study of histomorphology and immunohistochemistry. Light microscopic study and polymerase chain reaction test were carried out to compare the features and its expression. Analysis is carried out to find the difference, specificity and sensitivity. Results. Samples took within the first 8 hours following ER presentation showed severely deteriorated endothelial lining and both ofVE–cadherin and occludin dissociation. This endothelial junction disassembly was found in both of burned– and non–burned area; both of severe– and non– severe burn as well. In burned area, mRNA expression of VE–cadherin found to be increased, as occludin decreased. In severe burned group, mRNA expression of VE–cadherin as well as occludin found to be increased. VE–cadherin synthesis was found to be earlier than occludin. Conclusion. Dissociation of both of endothelial cell–to–cell molecules junction show no differences between the two groups, and between burned– and non–burned areas.

Myoglobin Expression in Chelonia mydas Brain, Heart and Liver Tissues RINI PUSPITANINGRUM; SEPTELIA INAWATI WANANDI; RONDANG ROEMIATI SOEGIANTO; MOHAMAD SADIKIN; DARYL ROBERT WILLIAMS; ANDREW ROBERT COSSINS
HAYATI Journal of Biosciences Vol. 17 No. 3 (2010): September 2010
Publisher : Bogor Agricultural University, Indonesia

An understanding of the underpinning physiology and biochemistry of animals is essential to properly understand the impact of anthropogenic changes and natural catastrophes upon the conservation of endangered species. An observation on the tissue location of the key respiratory protein, myoglobin, now opens up new opportunities for understanding how hypoxia tolerance impacts on diving lifestyle in turtles. The respiratory protein, myoglobin has functions other than oxygen binding which are involved in hypoxia tolerance, including metabolism of reactive oxygen species and of the vascular function by metabolism of nitric oxide. Our work aims to determine whether myoglobin expression in the green turtle exists in multiple non muscle tissues and to confirm the hypothesis that reptiles also have a distributed myoglobin expression which is linked to the hypoxiatolerant trait. This initial work in turtle hatch Chelonia mydas confirms the presence of myoglobin transcriptin brain, heart and liver tissues. Furthermore, it will serve as a tool for completing the sequence and generating an in situ hybridization probe for verifying of cell location in expressing tissues.

The aim of this research was to gain the profile of blood gasses of green turtle (Chelonia mydas) hatch. Blood gas of the green turtle was analysed after exposuring them at 28 oC and 50% of humidity for 24 hours in a pvc tube and at 34 oC under sunlight exposured with 47% of humidity for 30 minutes. The result showed the different values of blood gas contents. This result showed indication of metabolism activities and poikilothermic adaptation of green turtle hatch. This information can be used RINI PUSPITANINGRUM; SEPTELIA INAWATI WANANDI; RONDANG ROEMIATI SOEGIANTO; MOHAMAD SADIKIN
HAYATI Journal of Biosciences Vol. 18 No. 3 (2011): September 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.3.147

Isolation and Characterization of Chelonia mydas Myoglobin MOHAMAD SADIKIN; RINI PUSPITANINGRUM; SEPTELIA INAWATI WANANDI; RONDANG ROEMIATI SOEGIANTO
HAYATI Journal of Biosciences Vol. 19 No. 2 (2012): June 2012
Publisher : Bogor Agricultural University, Indonesia

Green sea turtle – Chelonia mydas is a lung respiration animal that is able to dive and stay uder sea water for hours without needing to surface for oxygen. As oxygen supply in muscles is assured by myoglobin, we propose to study some characteristic of this muscle protein in green sea turtles. To achieve this objective, pure green sea turtle myoglobin has to be made available. Therefore, our first task is to purify this muscle protein from green sea turtles. Skeletal muscles from 3 green sea turtle hatchlings were studied microscopically and biochemically. Microscopy observation showed a general structure of striated muscle. Biochemical studies revealed that green sea turtle myoglobin could be more purely isolated to a certain degree by Sephadex G-75 gel filtration and purified by immunoaffinity gel chromatography rather than direct purification by DEAE-Sepharose ion exchange chromatography technique. SDS-PAGE analysis showed that green sea turtle myoglobin ran together with horse myoglobin as 17 kDa molecular weight proteins

Properties of Folate Binding Protein Purified from Cow’s Milk . SUBANDRATE; DWIRINI RETNO GUNARTI; MOHAMAD SADIKIN
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Folic acid played an important role in the metabolism of the body. To measure the serum folic acid levels could use the folate binding protein (FBP) from cow’s milk with a technique analogous to ELISA. The aims of this study were to identify characteristics of FBP from cow’s milk and binding capacity of FBP to folic acid and to purify FBP from other whey protein passed through DEAE-cellulose chromatography column. Each of DEAE-cellulose peaks was passed in affinity chromatography column. FBP was released from affinity column with sodium acetate buffer pH 3.5. The purity of obtained FBP was demonstrated by a single spot in SDS-PAGE analysis and the estimated molecular weight of FBP was around 31 kDa. Our study indicated that 1 mol FBP bound 1 mol folic acid. Alkylation with iodoacetic acid decreased the binding capacity of FBP which suggested the presence of a–SH or imidazol group in its active site. The importance of disulfide bridge was proven by decreasing of folate binding capacity of FBP after b-mercaptoethanol treatment. In contrary, the folate binding didn need Ca2+ ion, as indicated by EDTA test which gave the same result as control.

Comparison of Hemoglobins from Various Subjects Living in Hypoxia RINI PUSPITANINGRUM; KURNIA NUZTIR MANTOLINI; . RUSDI; MOHAMAD SADIKIN
HAYATI Journal of Biosciences Vol. 20 No. 3 (2013): September 2013
Publisher : Bogor Agricultural University, Indonesia

The aim of this research was to obtain the different characteristics of haemoglobin molecules in subjects under hypoxic condition, namely eel, catfish, suckermouth fish, green sea turtle using an electrophoresis technique. We used human umbilical cord blood and thalassemia patient blood, as well as a normal adult-human blood as controls. The proteins obtained after electrophoresis process were stained with two different colouring techniques, each based on different principles. Both staining techniques gave practically identical results. Subject that live in hypoxic condition has a different haemoglobin in comparison to the one found in adult human live in normal oxygen condition (normoxia). These hypoxia-adapted or -needed hemoglobin migrate slower than adult human hemoglobin from normoxia. This observation suggests that hemoglobin which is needed to live in hypoxic condition or environment is a different molecule. Whether this hemoglobin from hypoxic condition has a higher affinity to oxygen is not yet known.

Characterization of Trypsin-Like Protease of Lactobacillus plantarum FNCC 0270 Trismilah Margono; Wahono Sumaryono; Amarila Malik; Mohamad Sadikin
HAYATI Journal of Biosciences Vol. 21 No. 2 (2014): June 2014
Publisher : Bogor Agricultural University, Indonesia

Trypsin is an enzyme that has a unique mechanism of cutting peptide bonds specifically at the carboxyl side of lysine or arginine amino acids, with another amino acid. This study aims to analyze a trypsin-like protease (TLP) found in Lactobacillus plantarum FNCC 0270, by performing partial proteomic tests, i.e. MALDI-TOF/TOF, and standard bioinformatics tools. SDS-PAGE analysis showed 4 protein bands. Two bands of the (P1 and P2) showed molecular weights equivalent to 47.35 and 38.42 kD, each generating 8 and 11 peptide fragments respectively. According to information in www.ncbi.nlm.nih.gov/genbank/structures, the structure of serine protease HtrA (subs. plantarum L. plantarum ST–III) consists of three domains. Using Clone Manager® software by aligning two sequences we obtained eleven. The Lactobacillus produces of the trypsin-like serine protease has 40-90% similarity. Using the Clustal W2 software we passed the 11 sequences through multiple alignments, and found that the isolate L. plantarum is closely related to L. buchneri, L. brevis, and L. malefermentans on the phylogenetic tree. Alignment analysis results showed that all 8 peptide fragments of band 1 and 11 peptide fragments of band 2, of the SDS-PAGE, were located in the active domain region of the fourth trypsin-like serine protease producing Lactobacilli.

Expression of Hypoxia-Inducible Factor-1α and Myoglobin in Rat Heart as Adaptive Response to Intermittent Hypobaric Hypoxia Exposure Margaretha Herawati; . Wardaya; Wawan Mulyawan; Fanny Septiani Farhan; Frans Ferdinal; Sri Widia A. Jusman; Mohamad Sadikin
HAYATI Journal of Biosciences Vol. 24 No. 3 (2017): July 2017
Publisher : Bogor Agricultural University, Indonesia

The aim of this study was to investigate the influence of intermittent hypobaric hypoxia on the expression hypoxia adaptation proteins, namely hypoxia inducibla factor-1a (HIF-1a) and myoglobin (Mb). Twenty five male Sprague-Dawley rats were exposed to intermittent hypobaric hypoxia in a hypobaric chamber in Indonesian Air Force Institute of Aviation Medicine, for 49.5 minutes at various low pressure, 1 week interval for 4 times (day 1, 8, 15 and 22). HIF-1α and Mb protein were measured with ELISA. mRNA expression of Mb was measured with one step real time RT-PCR. HIF-1α protein levels increased after induction of hypobaric hypoxia and continues to decrease after induction of intermittent hypobaric hypoxia 3 times (ANOVA, p = 0.0437). mRNA expression and protein of Mb increased after induction of hypobaric hypoxia and continues to decrease after induction of intermittent hypobaric hypoxia 3 times (ANOVA, p = 0.0283; 0.0170), and both are strongly correlated (Pearson, r = 0.6307). The heart of rats adapted to intermittent hypoxia conditions by upregulation the expression of HIF-1a and myoglobin and then both return to normal level.

Deskriptif Histologi Fase Wound Healing (Penyembuhan Luka) Pada Regenerasi Jaringan Ekor Cecak (Hemidactylus platyurus) Titta Novianti; Mohamad Sadikin; Vetnizah Juniantito; Sri Widia A Jusman; Evy Ayu Arida
Prosiding Seminar Biologi Vol 2 No 1 (2016): Prosiding Seminar Nasional From Basic Science to Comprehensive Education
Publisher : Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/psb.v2i1.3305

Saat terjadi kerusakan pada jaringan setiap organisme, maka tubuh akan selalu berusaha untuk memulihkan kerusakan pada jaringan. Pada organisme multiseluler, regenerasi tidak hanya pemulihan struktur tetapi juga terjadi pemulihan fungsi jaringan. Proses regenerasi jaringan terjadi dalam empat tahap yang melibatkan berbagai jenis sel. Tahap pertama regenerasi adalah fase wound healing (penyembuhan luka), yang merupakan fase penentu berhasilnya proses regenerasi jaringan. Hasil penelitian secara deskriptif histologi dengan pewarnaan konvensional hematoksilin eosin, menggunakan model ekor cecak, terdapat berbagai jenis sel yang muncul dan berperan dalam proses regenerasi fase tersebut. Pada hari pertama dan hari ketiga setelah proses autotomi ekor cecak, tampak sel darah merah akibat perlukaan dan sel darah heterofil yang berperan mem-fagositosis sel dan jaringan yang mati, serta munculnya sel fibroblast. Sel fibroblast ini yang akan berperan dalam pembentukan jaringan baru. Pada hari kelima tampak pembentukkan pembuluh darah dan otot yang baru, serta pada hari kesepuluh terjadi pembentukkan lapisan sel epitel dan epidermis. Tahap regenerasi hari pertama sampai hari kesepuluh merupakan tahap penyembuhan luka. Setelah luka tertutup,mulai terjadi pertumbuhan berbagai jaringan di bawah epidermis. Kata kunci: regenerasi jaringan, histologi, ekor, cecak (Hemydactylus platyurus)

Co-Authors . RUSDI . Rusdi Ahmad A. Jusuf Ahmad Aulia Ahmad Aulia Jusuf Ahmad R. Utomo Aldri Frinaldi Amarila Malik Andi N.K. Syarifin, Andi N.K. ANDREW ROBERT COSSINS Angelina Stevany Regina Masengi Ani R. Prijanti Ani Retno Prijanti Ardiana Kusumaningrum Arfat Lusinanto Arief Budi Witarto Aryenti . Asri Rasad ASTUTIATI NURHASANAH Astutiati Nurhasanah Bethy S. Hernowo Caroline T. Sardjono CAROLINE TAN SARDJONO Cicia Firakania, Cicia DARYL ROBERT WILLIAMS Dewi Sukmawati Diah Handayani Diah Iskandriati Dian R. Laksmitawati Dian Ratih Laksmitawati Dwirini Retno Gunarti Dwirini Retno Gunarti Ekawati, Maria Evy Ayu Arida Fanny Septiani Farhan Febriana C. Iswanti Febriana Catur Iswanti Franciscus D. Suyatna Frans Ferdinal Frans Ferdinal HANIFAH RAHMI Hans -Joachim Freisleben Hans Joachim Freisleben HANS-JOACHIM FREISLEBEN Heri Wibowo Hermansyah Hermansyah Ichramsyah A. Rahman Ika Superti Daruningrum Indra G. Mansur Indra Gusti Mansur Irawan Satriotomo Jeanne A. Pawitan Jeanne Adiwinata Pawitan Juniarti . KURNIA NUZTIR MANTOLINI Lailan Safina Nasution Lindi G Haritsyah M, Mentari Mohamad Saekhu Muchlis Ramli Muhamad Arif Budiman Muhamad Yusup Muhammad Faris Indratmo Nani Dharmasetiawani Nani Sari Murni Ni Ken Ritchie Nina Fitriana, Nina Ninik Mudjihartini Noorwati Sutandyo Novi S. Hardiany Novi Silvia Hardiany Novianti, Titta Nurhadi Ibrahim Nurhadi Ibrahim Nurjati C. Siregar Oentoeng Soeradi Pamungkas, Joko Panjaitan, Paris Praditi, Citra Radiana D. Antarianto Rahmawati Ridwan Rahmawati Ridwan Ranti Dwi Astriani Reni Paramita Resda Akhra Syahrani Ria Syafitri Evi Gantini Rini Puspitaningrum RONDANG ROEMIATI SOEGIANTO RONDANG ROEMIATI SOEGIANTO RONDANG ROEMIATI SOEGIANTO Rostika Flora Sadikin, Abdul Halim Saleh, Mgs. M. Irsan Samsuridjal Djauzi Sari, Dewi H. SARI, MIKE PERMATA Sarmoko Sarsanti, Pungguri Ayu Nega Sekar Arumsari Septelia I. Wanandi SEPTELIA INAWATI WANANDI Siregar, Nurjati Siti Rahmawati Achyat Sitorus, Novandrik Yeriko Siufui Hendrawan Sri S. Ningsih, Sri S. Sri W.A. Jusman Sri Widia A.Jusman Subandrate Suryani, N. Lilis Syarifah Dewi Syazili Mustofa Tiara Mayang Pratiwi Lio Tiwuk Susantiningsih Tomohiko Yamazaki Trismilah Margono TRISMILAH TRISMILAH Trismilah Trismilah Trismilah, Uly A. Nikmah, Uly A. Vetnizah Juniantito Wahono Sumaryono Wardaya Wardaya Wawan Mulyawan Wawan Mulyawan Wawan Mulyawan Wawan Mulyawan Wawan Mulyawan Wawan Mulyawan Wiwoho, Yudi Yuwono Yalista Fatia Nadia Yefta Moenadjat Yudianita Kesuma, Yudianita Yuhernita . Zen Hafy

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