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All Journal HAYATI Journal of Biosciences Jurnal Hortikultura Indonesia (JHI) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Bumi Lestari Zuriat Jurnal Ilmiah Aplikasi Isotop Radiasi BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Pelita Perkebunan AGRIVITA, Journal of Agricultural Science Jurnal Agroekoteknologi Indonesian Journal of Biotechnology Biology, Medicine, & Natural Product Chemistry Bioeksperimen: Jurnal Penelitian Biologi Journal of Tropical Biodiversity and Biotechnology ANNALES BOGORIENSES Jurnal Pertanian Agros Biosaintifika: Journal of Biology & Biology Education Scripta Biologica Jurnal Riset Biologi dan Aplikasinya JURNAL ILMIAH AGRINECA Agrosaintifika: Jurnal Ilmu-Ilmu Pertanian Annales Bogorienses
Endang Semiarti
Fakultas Biologi, Universitas Gadjah Mada, Indonesia
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Journal : Scripta Biologica

 1 
PENGARUH PEPTON TERHADAP PERTUMBUHAN EMBRIO ANGGREK Vanda tricolor Lindley var. Suasiv ASAL MERAPI SECARA IN VITRO Rizka Riliant Puspasari; Ikhsanudin Nur Rosyidi; Eka Fitriana Candra Ningrum; Endang Semiarti
Scripta Biologica Vol 5, No 1 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (944.029 KB) | DOI: 10.20884/1.sb.2018.5.1.762

Abstract

Orchidaceae is a valuable ornamental plant in Indonesia’s export commodities. The growth of orchid in nature is prolonged due to its microscopic size of the seed and has no endosperm. In the mass-production, an inducer is required to accelerate the growth in an in-vitro culture, one of which is peptone. The embryos used in this study is Merapi endemic orchid, the Vanda tricolor Lindley var. Suavis. This study aimed to determine the effect of peptone on the growth of eight weeks old of Vanda tricolor Lindley var. Suavis embryos and to determine the optimal peptone concentration to induce the growth of the orchid embryo. This experiment was conducted in the Biotechnology Laboratory of the Faculty of Biology Universitas Gadjah Mada from September to October 2016. The treatments applied in this study was a variety of peptone concentrations (0–3 g/L) in growth media to induce eight weeks old protocorm subcultures. The study measured the morphological development of protocorm to determine the effect. The results showed a positive effect of peptone on the growth of orchid embryos at the NP1 treatment concentration of 1 g/L. The growth reached the phase 6 with the rate measured about 6.85±0.001% in the fourth week. This value was higher than the protocorm growth rate when it reached the phase 6 in the fourth week on the NP0 medium measured at 5.22±0.003%, on the medium NP2 at 1.15±0.002%, and on the medium NP3 at 1.02±0.001%. This result showed the effect of peptone concentration to induce the growth of Vanda tricolor Lindley var. Suavis embryo, but the excess concentration of peptone inhibited the growth of protocorm.
Co-Authors , Surifah A.A. Ketut Agung Cahyawan W Achmadi Priyatmojo Aditya Nur Subchan Agus Slamet Ahmad Suyoko Alisa Julia Nurulita Alydarafa, Hafshah Aminatun Munawarti Aminatun Munawarti, Aminatun Amru Rizal Basri Anami Riastri Andani Kesuma, Amarilis Annisa, RR Rifka Ari Indrianto Ari Indrianto Ari Indrianto Ari Indrianto Ari Indrianto Ari Indrianto Ari Indrianto Aries Bagus Sasongko Arimarsetiowati, Rina Arum, Dyah Ayu Puspita Asri Fajar Milasari Azis Purwantoro Azis Purwantoro Aziz Purwantoro Bekti Sulistya Utami Binti Tsulsiyah Brilliant Kharisma Apritadila BUDI SETIADI DARYONO Cahya Lembayung Sutra Catharina Tri Widyastuti Christanti Sumardiyono Christanti Sumardiyono Della Rosiana Ningtias Devi Bunga Pagalla Dewi Tika Sari Dewi Yuliana Rizqi Dika Sundari Eka Fitriana Candra Ningrum El Hakim, Alim Erwin Prastowo Evilili Usmanti Exsyupransia Mursyanti Exsyupransia Mursyanti Exsyupransia Mursyanti Faiza Senja Widya Perdana Falah Nur Alifianto Febri Yuda Kurniawan Fhea Putri Cristy Fitriana Puspitasari Frisca Damayanti Gde Cahyadi Wirajagat Gildantia, Elke Hanifa, Yumna Rahmadias Ikhsanudin Nur Rosyidi Ixora Sartika Mercuriani JAKA WIDADA Jose Gutierrez-Marcos Jose Gutierrez-Marcos Kana Ninomiya Kinasih, Anggiresti Kurniawan, Febri Yuda Linggabuwana, Aviesta Maryani Maryani Matsumoto, Shogo Maura Indria Meidianing Meidianing, Maura Indria Melati, Chrisnanda Ayu Muhammad Dylan Lawrie Muhammad Dylan Lawrie Muhammad Dylan Lawrie Muhammad Dylan Lawrie Naufal Ghozi Aditya Perdana Ni Luh Putu Kayika Febryanti Nintya Setiari Nintya Setiari Nopitasari, Sri Oedjijono Oedjijono, Oedjijono Oktaviana Herawati Pauline Destinugrainy Kasi Prasojo, Ireneus Seno Pratiwi, Apriliana Putra, Rinaldi Rizal Putri, Saifa Usni R Soelistijono Rahayu Sulistianingsih Ramadhani, Aulia Noor Rasjid, Nuzlan Rinaldi Rizal Putra, Rinaldi Rizal Rindang Dwiyani Rizka Riliant Puspasari Rumiyati Rumiyati Rumiyati, Rumiyati Seonghoe Jang Setiaji, Arkan Shogo Matsumoto Sismindari . Sismindari Sismindari Sismindari Sismindari Sitarina Widyarani Soenghoe Jang Sri Nopitasari Sri Wahyuningsih Stalis Norma Ethica Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sulastri Isminingsih Taryono, Taryono Thoyibatul Farida Tri Joko Raharjo Triono Bagus Saputro, Triono Bagus Umi Kulsum Nur Qomariah Windi Mose Windi Mose Woerjono Mangoendidjojo Woro Anindito Sri Tunjung Yasushi Yoshioka Yohana Theresia Maria Astuti, Yohana Theresia Maria Yoshioka, Yasushi Yuli Setiawati Yuuki Asano Zulfa Layina