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All Journal Jurnal Fitopatologi Indonesia Agrikultura Jurnal Ilmu-Ilmu Pertanian Indonesia AGRIVITA, Journal of Agricultural Science JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA AGRITROPICA : Journal of Agricultural Sciences Martabe : Jurnal Pengabdian Kepada Masyarakat Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Akta Agrosia Dharma Raflesia : Jurnal Ilmiah Pengembangan dan Penerapan IPTEKS Jurnal AGRISEP: Kajian Masalah Sosial Ekonomi Pertanian dan Agribisnis Buletin Dharmas Andalas
Mimi Sutrawati
Universitas Bengkulu
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Electrical & Electronics Engineering Library & Information Science Medicine & Pharmacology Social Sciences Other

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Journal : AGRITROPICA : Journal of Agricultural Sciences

 1 
First Report of Banana Bunchy Top Disease on Banana in Bengkulu. Mimi Sutrawati; Sempurna Ginting
AGRITROPICA : Journal of Agricultural Sciences Vol 3, No 2 (2020)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/j.agritropica.3.2.82-87

Abstract

Banana is a horticulture crop that has economic value and is widely cultivated in tropical countries. Banana production in Bengkulu province reached 259,748 quintals, then durian (110,387 quintals), tangerines (94,396 quintals) (BPS 2015). Banana bunchy top disease caused by Banana bunchy top virus (BBTV) infection is considered the most crucial virus disease affecting yield losses of a banana plantation in Asia, Africa, and the South Pacific. However, the incidence and molecular characters of BBTV has never been reported in Bengkulu. This research aims to characterize symptom variations, disease incidence, and disease severity of BBTV infection in Bengkulu and virus detection using molecular methods by polymerase chain reaction (PCR). Disease incidence of BBTV was measured based on field symptoms. The disease survey was conducted in Bengkulu city, Bengkulu Utara district, and Rejang Lebong district. The study showed that the incidence of BBTV in Bengkulu City, Bengkulu Utara, and Rejang Lebong ranged from 0% to 100%. The most common symptoms observed in the field involved vein clearing, upturned leaf, chlorotic, and ragged margins, reducing petiole length, distance, lamina width, and stunting. Banana crops that are infected with BBTV in the vegetative phase will not produce fruit. In contrast, viral infection in the generative phase causes the formation of stunted fruit that is not suitable for harvesting. Thus, the potential loss of yield due to stunted disease can reach 100%. This study's results are the first reports of BBTV infection in banana crops in Bengkulu. Disease diagnosis will form the basis of disease control strategies in banana crops.
Response of Cucumber (Cucumis sativus L.) Varieties Against TMV (Tobacco mosaic virus) Infection Witanto Madyo Utomo; Mimi Sutrawati; Eko Supriyono
AGRITROPICA : Journal of Agricultural Sciences Vol 4, No 2 (2021)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/j.agritropica.4.2.129-135

Abstract

The tobacco mosaic virus (TMV) is a Tobamovirus that can infect cucumber plants. There is limited information about the response of cucumber varieties against TMV infection. The research was conducted to evaluate the response of cucumber varieties to TMV infection using TMV isolates from Bogor. The evaluation of resistance to TMV isolates was conducted by sap transmission method using TMV isolates from Bogor that propagated on tobacco as the source of inoculum. Variable observations covered the period of incubation (the day after inoculation/DAI), the symptoms of the disease, disease incidence, and disease severity. The presence of viral infection was observed through the accumulation of starch and inclusions bodies. Based on the results of the research, the TMV isolate was able to infect systematically all tested plants with symptoms of which appear in the form of mosaic light to heavy with the incidence of the disease reaching 100%. Response of cucumbers varieties to TMV isolate can be categorized into resistant (Bandana F1, Neptun, and Pandu), tolerant (Ethan F1), and susceptible (Vario F1).
Virus Identification in Yard Long Bean Plants with Yellow Mosaic Symptoms Sutrawati, Mimi; Aulia, Ewa
AGRITROPICA : Journal of Agricultural Sciences Vol. 7 No. 2 (2024)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/j.agritropica.7.2.116-121

Abstract

Begomovirus infection, which causes yellow leaf curl disease, is commonly found in the Bengkulu province, affecting various plants, including chilli, melon, cucumber, papaya, and weeds. The viruses reported to date in long bean plants include Mungbean yellow mosaic India virus (MYMIV) and Bean common mosaic virus (BCMV). This study aimed to detect viruses in long bean samples collected from Musi Rawas (South Sumatra) and North Bengkulu (Bengkulu), areas where yellow mosaic symptoms resembling those caused by Begomovirus infection were observed. Virus detection was performed using PCR-based DNA analysis with specific MYMIV (MY1/MY2) primers, targeting a DNA fragment of approximately 238 bp. The results revealed that yard-long bean plants from Musi Rawas were positively infected with MYMIV, while those from North Bengkulu tested negative for MYMIV. Begomovirus or other viral infections may cause the yellow mosaic symptoms observed in North Bengkulu yard long beans. Therefore, further investigations using additional primers are needed to identify the specific virus responsible for the symptoms in this region.
Genetic Diversity of Papaya Using Molecular Markers Random Amplified Polymorphic DNA Aulia, Ewa; Sutrawati, Mimi; Simarmata, Marulak
AGRITROPICA : Journal of Agricultural Sciences Vol. 7 No. 1 (2024)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/j.agritropica.7.1.17-22

Abstract

Papaya is one type of fruit that is widely consumed and cultivated by the farmer. However, genetic analysis has not been carried out on various types of papaya available on the market. This aims to determine the genetic diversity of papaya plants that can become genetic resources to fullfil food needs and genetic resources for breeders. Genetic analysis was conducted by Random Amplified Polymorphic DNA (RAPD) method using 11 papaya varieties consisting of Calina, Bangkok, Red Pomegranate, Sunrise, Orange Lady, Red Lady, Taiwan, Arum, Miba, Golden, and Local and using 15 RAPD primers consisting of OPA-1, OPA-2, OPA-8, OPA-16, OPC-4, OPC-11, OPC-13, OPC-20, OPD-20, OPE-2, OPE-6, OPE-11, OPE-14, OPM-6, and OPY-15. PCR-RAPD results were translated into binary data and then cluster analysis was conducted using the Unweighted Pair-Group Method Arithmetic (UPGMA) method using the Numerical Taxonomy and Multivariate System (NTSYS) program. The PCR-RAPD results of 11 papaya varieties successfully amplified 8 out of 15 primers that formed 112 DNA bands with 85 polymorphic bands. Genetic diversity analysis showed the results at 90% similarity coefficient formed 9 groups. Group 1 consists of Bangkok and Sunrise varieties. Group 2 consists of Red Pomegranate and Arum varieties. Groups 3 to 9 consisted of Calina, Miba, Local, Golden, Orange Lady, Red Lady, and Taiwan varieties, respectively.
Co-Authors Abdul Fiddin Agustin Zarkani Alamria, Intan Suryani ALI NURMANSYAH Andreani Kinata Anwar, Guswarni Apri Andani, Apri Atra Romeida Aulia, Ewa Bonny Purnomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Djamilah Djamilah . Djamilah Djamilah, Djamilah Dwi Wahyuni Ganefianti Dwi Wahyuni Ganefianti Dwi Wahyuni Ganefianti, Dwi Wahyuni Eko Supriyono Ewa Aulia Fausiah T. Ladja Gede Suastika Gede Suastika Hamidi, Ilmi Haquarsum, Eka J.V. Hendri Bustamam Hendri Bustamam Herison, Catur Listihani, Listihani Manullang, Yedija Marlin Marlin Marpaung, Nia Kurniati Br. Marulak Simarmata Merakati Handajaningsih Nabillah, Ade Nadrawati, Nadrawati Novanda, Ridha Rizki Nyayu Neti Arianti Nyayu Neti Arianti Nyayu Neti Arianti, Nyayu Neti Parwito Parwito, Parwito Priyatiningsih Priyatiningsih Priyatiningsih Reny Herawati Rustikawati Rustikawati Sari, Icha Kurnia Nanda Selangga , Dewa Gede Wiryangga SEMPURNA GINTING Sipriyadi Sitohang, Enjelina Br Sobir Sobir SRI HENDRASTUTI HIDAYAT Sukisno Sukisno, Sukisno Sura Menda Ginting Tunjung Pamekas Tunjung Pamekas, Tunjung Ulfah Anis Witanto Madyo Utomo Wuri Prameswari Yenny Sariasih Yenny Sariasih