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All Journal PIRAMIDA Buletin Veteriner Udayana Buletin Udayana Mengabdi Majalah Ilmiah Peternakan Jurnal Veteriner Media Kedokteran Hewan ARSHI Veterinary Letters Advances in Tropical Biodiversity and Environmental Sciences Jawa Dwipa Journal of Veterinary and Animal Sciences
Ni Ketut Suwiti
Fakultas Kedokteran Hewan Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary

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The Expression Of Cd4+ Lymphocytes Of Bali Cattle After Consuming Mixed Mirerals Ni Ketut Suwiti; M Windhu; Ni Luh Watiniasih; I Nengah Kerta Besung; I Nyoman Suartha
Advances in Tropical Biodiversity and Environmental Sciences Vol 1 No 2 (2017): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (480.196 KB) | DOI: 10.24843/ATBES.2017.v01.i02.p04

Abstract

Minerals play an important role in activating the lymphoid cells. Mineral deficiency can cause interference prolifrasi in lymphocytes, particularly the expresion of CD4+. Bali cattle are hardly given additional mineral due to cattles are in semi-intensive breeding method. Therefore, this study aims to determine the expression of CD4+ lymphocytes Bali cattle after consuming of mixed mineral. The samples used were 32 male bali cattles reared in the village of Catur, Kintamani, Bangli regency. Samples were divided into 2 groups, those were: 16 individul of bali cattle was given 7.5gr additional of mixed mineral per individual/day, and theother 16 individual as acontrol. The treatmnes were conducted for 3 months. The results showed that administration of 7.5gr mixed mineral per individual/day did not show differences in the expression of CD4+ lymphocyte of bali cattle.
Gambaran Histologi Ginjal Ayam Broiler yang Diberi Infusa Daun Dadap Setelah Mengalami Stress Pengangkutan Muh Amiruddin; Ni Ketut Suwiti; Anak Agung Gde Arjana
Buletin Veteriner Udayana Vol. 14 No. 6 December 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i06.p14

Abstract

The transportation of broiler chickens can cause stress to broiler chickens resulting in a decrease in body weight and high mortality. Stress causes disturbances, causing changes in the organs that make up the chicken body, one of which is the kidneys. This study aims to determine the effect of infusion of Dadap (Erythrina Subumbrans) leaves as an antioxidant in broiler chickens subjected to transport stress at 33°-35°C for 4 hours. This study used a completely randomized design (CRD) with a 3x2x5 factorial pattern with three replications. The number of samples used were 30 broiler chickens which were divided into 5 treatment groups, namely P0: negative control; P1: positive control and given vitamin C 2 gram/L; P2: 1000 ppm of Dadap leaf infusion is given; P3: 2000 ppm of Dadap leaf infusion was given and P4: 3000 ppm of Dadap leaf infusion was given. In the eighth result, after being given transport stress treatment, the kidneys were taken and then histological preparations were made with Hematoxylin Eosin (HE) staining. Parameters observed were degeneration, necrosis and infiltration of inflammatory cells. Histological scoring data were then analyzed using the Kruskal-Wallis test, if there was a significant difference followed by the Mann-Whitney test. The results showed that giving dadap leaf infusion to broiler chickens that were not given stress reduced necrosis (P<0,01) while stress-treated chickens reduced necrosis (P<0,01) and inflammatory cell infiltration (P<0,05). It was concluded that the administration of 10% dadap leaf infusion with a concentration of 2000 ppm and 3000 ppm could improve the histological picture of the kidneys of broiler chickens experiencing transport stress.
Identification of Growth Hormone Gene of Bali Cattle with Qualitative Superior in Bali Province Ni Ketut Suwiti; I Wayan Suardana; Ni Luh Watiniasih; I Nengah Kerta Besung; Chandra Yowani
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p09

Abstract

A research has conducted, in order to identify the genetic markers of Bali cattle that can be used as an indicator of their qualitative superior. The genetic marker identified was the growth hormone gen. As many as 50 whole blood samples which were collected from 50 individual cattle which were certificated as good breed or had qualitative superior, were used in this study. The DNA fragments were identified by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method with primers, GH5 (5’-CCC ACG GGC AAG AAT GAG GC-3’ and GH6 (5’TGA GGA ACT GCA GGG GCC CA-3), respectively. Furthermore, the PCR products were restricted with MspI endonucleic restriction enzyme, before electrophoresis in 2% agarose The result showed that the growth hormone gene of bali cattle with qualitative superior has polymorphism which characterized by the formation of two fragments i.e. 230 and 329 bp that categorized as: allele +/+ and allele -/-.
Gambaran Histologi Kulit Bagian Abdomen dan Profil Total Leukosit Anjing yang Menderita Dermatitis Ni Wayan Ayu Rukmini; Ni Ketut Suwiti; Nyoman Sadra Dharmawan
Buletin Veteriner Udayana Vol. 15 No. 6 December 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i06.p23

Abstract

Skin is the markers of dog health and damage/lesions on the skin cause the dog appearance to be unattractive. One of the diseases that affect the appearance of dogs is dermatitis. Dermatitis is an infection that attacks the skin organs and tends to be difficult to cure, especially infections that occur in the abdominal area. Therefore, in its treatment, it is necessary to know the level of changes in skin lesions, as well as the patient's blood profile. This study aims to determine the histology of the skin and the total leukocyte profile of dogs with dermatitis and compared with non-dermatitis. 24 samples were taken from dogs with dermatitis and non-dermatitis. The samples form abdominal skin tissue and whole blood were taken by purposive sampling method. Histological images were examined using a microscope (400x) with the Haris haematoxilin eosin staining method, while the total leukocyte profile was measured using a hematology analyzer. The results showed that the clinical symptoms of dogs suffering from dermatitis were characterized by: itching, redness/rubor, hair loss/alopecia. Epidermal skin histology found: hyperkeratosis of the stratum corneum, necrosis, inflammatory cell, hydrophic and spogiotic degeneration of keratocyte cells, hyperplasia of the stratum granulosum. There is a segment s. scabiei in hair follicles, infiltration of lymphocytes and neutrophils, folliculitis and furunculosis in the dermis and hypodermis layers. While the leukocyte profile was found to increase, namely in dogs with dermatitis as much as 59% and non-dermatitis 50%. It can be concluded that dogs with dermatitis experience changes in histology and total leukocytes. However, in the future studies it is advisable to classify the types of dermatitis and differential leukocytes.
STRUKTUR DAN MORFOMETRI LIMPA ITIK BALI (Anas sp.) PADA FASE PERTUMBUHAN Winda Ara Yulisa; Ni Luh Eka Setiasih; Luh Gde Sri Surya Heryani; Ni Ketut Suwiti; Ni Nyoman Werdi Susari; I Gede Soma
Jurnal Veteriner Vol 24 No 3 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.3.357

Abstract

Limpa merupakan organ yang dikelompokkan ke dalam sistem limfoid sekunder. Limpa memiliki fungsi imunitas terhadap antigen yang masuk ke dalam tubuh dan menghancurkan eritrosit yang rusak. Penelitian ini bertujuan untuk mengetahui struktur dan morfometri limpa itik bali (Anas sp.) pada fase pertumbuhan/grower. Penelitian ini menggunakan 20 ekor itik bali yang dibagi menjadi dua kelompok yaitu jantan dan betina yang masing-masing terdiri atas 10 ekor (umur 2-3 bulan). Hasil data struktur anatomi dan histologi dianalisis secara deskriptif kualitatif, sedangkan data morfometri dan histomorfometri untuk menguji perbedaan antara jantan dan betina digunakan uji Independent sample T-test dengan prosedur analisis menggunakan program SPSS versi 26. Struktur anatomi limpa itik bali berbentuk segitiga piramid dan berwarna cokelat kemerahan, struktur histologi limpa itik bali terdiri atas kapsula, trabekula, pulpa merah dan pulpa putih. Hasil pengukuran morfometri berat limpa itik bali jantan diperoleh 0,68 ± 0,20 g dan berat limpa itik betina 0,66 ± 0,24 g. Volume limpa itik bali jantan dan betina berturut-turut 0,60 ± 0,19 mL dan 0,58 ± 0,23 mL. Hasil pengukuran histomorfometri ketebalan kapsula limpa itik jantan dengan betina adalah 17,97 ± 4,81 ?m; 31,75 ± 6,09 ?m; ketebalan trabekula jantan 17,20 ± 3,26?m ; dan betina 22,54 ± 6,29 ?m; serta diameter pulpa putih jantan adalah 214,69 ± 14,77 ?m; dan diameter pulpa putih betina adalah 199,56 ± 23,58 ?m. Simpulan penelitian ini adalah limpa itik bali jantan dan betina pada fase pertumbuhan/grower memiliki struktur anatomi dan histologi yang sama, morfometri yang tidak berbeda nyata, serta histomorfometri yang menunjukkan perbedaan pada ketebalan kapsula dan trabekula sedangkan diameter pulpa putih tidak jauh berbeda.
Struktur dan Morfometri Ginjal Itik Bali (Anas sp.) pada Fase Pertumbuhan Ni Putu Dewi Setia Sari; Ni Luh Eka Setiasih; Luh Gde Sri Surya Heryani; Ni Ketut Suwiti; Ni Nyoman Werdi Susari; I Ketut Suatha
Jurnal Veteriner Vol 24 No 3 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.3.374

Abstract

Ginjal merupakan organ ekskresi yang berperan dalam membuang zat sisa metabolisme yang tidak dibutuhkan lagi di dalam tubuh. Penelitian ini bertujuan untuk mengetahui struktur dan morfometri ginjal itik bali (Anas sp.) dengan jenis kelamin berbeda pada fase pertumbuhan/ grower. Penelitian menggunakan 32 ekor itik bali yang dibagi menjadi dua kelompok jenis kelamin masing-masing 16 ekor (umur 2-3 bulan). Hasil data struktur dianalisis dengan deskriptif kualitatif,sedangkan data morfometri digunakan uji Independent sample T-test dengan program SPSS. Hasil pengukuran ginjal itik bali jantan diperoleh panjang ginjal kanan 7,256 + 0,388 cm, panjang ginjal kiri 7,175 + 0,437 cm, bobot ginjal kanan 3,50 + 0,61 g, bobot ginjal kiri 3,487 + 0,497 g, volume ginjal kanan 0,350 + 0,103 cm3, volume ginjal kiri 0,362 + 0,088 cm3, glomerulus tipe mamalia 34,025 + 2,931 ?m dengan lebar bowman 8,173 + 2,447 ?m, glomerulus tipe reptil 14,777 + 2,300 ?m dengan lebar ruang bowman 6,676 + 1,780 ?m. Hasil pengukuran ginjal itik bali betina diperoleh panjang ginjal kanan 6,812 + 0,263 cm, panjang ginjal kiri 6,781 + 0,299 cm, bobot ginjal kanan 4,012 + 0,464 g, bobot ginjal kiri 3,987 + 0,401 g, volume ginjal kanan 0,418 + 0,116 cm3, volume ginjal kiri 0,393 + 0,106 cm3, glomerulus tipe mamalia 43,443 + 4,686 ?m dengan lebar bowman 9,068 + 3,483 ?m, glomerulus tipe reptil 23,312 + 2,761 ?m dengan lebar ruang 6,390 + 1,995 ?m. Hasil pengujian terhadap panjang bobotginjal dan ginjal menunjukkan berbeda nyata P<0,05, sedangkan volume ginjal tidak berbeda nyata P>0,05. Hasil pengujian terhadap glomerulus tipe mamalia dan glomerulus tipe reptil menunjukkan berbeda nyata P<0,05. Struktur anatomi danhistologi antara ginjal itik bali jantan dan betina adalah sama, sedangkan morfometri anatomi dan histologi ginjal itik bali jantan dengan betina berbeda.
IDENTIFIKASI DAGING SAPI BALI DENGAN METODE HISTOLOGIS Suwiti, Ni Ketut
Majalah Ilmiah Peternakan Vol 11 No 1 (2008)
Publisher : Fakultas Peternakan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (423.601 KB)

Abstract

ABSTRAK Telah dilakukan penelitian identifikasi daging sapi bali dengan metode histologis yakni melakukan pengamatan terhadap struktur mikroskopis. Sampel daging sapi bali. berupa muskulus pectoralis profundus, diperoleh dari pasar swalayan di Denpasar. Sampel difiksasi, didehidrasi dan diembedding dalam parafin selanjutnya dilakukan pemotongan dengan mikrotom ketebalan 4 - 5 µ. Dibuat sediaan histologis dengan metode pewarnaan Harris-Haematoxilin-Eosin. Pengamatan terhadap struktur histologi dilakukan dengan mikroskop cahaya binokuler pembesaran 450x. Hasil penelitian menunjukkan struktur histologi daging sapi bali terdiri atas serabut otot skelet longitudinal dan tranversal dengan multinuklleus. Nukleus terletak dipinggir sel. Ditemukan jaringan ikat padat dan jaringan lemak dengan pembuluh darah. Diameter serabut otot berukuran 8,40 ± 1,41 µm. Pada pengamatan histologis juga ditemukan : endomisium, perimisium dan epimisium IDENTIFICATION OF BALI CATTLE MEAT WITH HISTOLOGICAL METHODS ABSTRACT A study to identification the microscopic structure of bali cattle meat by histological methods, has been carried out. The meat bali cattle samples were collected from musculus pectoralis profundus has been taken from Denpasar supermarket. The tissue samples were fixed, dehydrated and embedded in paraffin and 4 - 5 µ. sections. Harris-Haematoxilin-Eosin staining method, using to identified of histological structure. Microscopic analysis was performed using a binocular light microscope (450X). The study showed that, histological structure of bali cattle beef was composed by longitudinally and transverselly skeletal muscle with a multinucleated. The nuclei in the periphery of the cell, there are dense connective tissue, fat, with small blood vessels. The skeletal myofibers diameter of muscle is 8.40 ± 1,41 µm. We observed for the presence of : endomysium , perimysium and epimysium.
Case Report: Ehrlichiosis and Anaplasmosis in Timber Wolf Crossbreed (Canis lupus) in Bali, Indonesia Widyasanti, Ni Wayan Helpina; Putra, I Putu Cahyadi; Suwiti, Ni Ketut
Media Kedokteran Hewan Vol. 36 No. 1 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i1.2025.88-99

Abstract

Pathogenic bacteria from the Anaplasmataceae family cause ehrlichiosis and anaplasmosis in animals, including dogs and wild carnivores (wolves, foxes, raccoons, and others). These diseases are emerging vector-borne diseases transmitted through ticks. A six-month-old timber wolf crossbreed (Canis lupus) came to the Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Udayana University, Bali, Indonesia, with a history of weakness, decreased appetite, and excessive salivation. Examination revealed that the timber wolf crossbreed had pale mucosa, lethargy, hypersalivation, normochromic microcytic anemia, thrombocytopenia, gas accumulation in the stomach and intestine, and no foreign bodies in the digestive tract. Rapid test results with the SNAP® 4Dx® Plus Test IDEXX® were positive for Ehrlichia sp. and Anaplasma sp.; however, the blood smear examination was negative. The therapy for this case included sodium chloride 0,9% infusion as fluid therapy, atropine sulfate as symptomatic therapy, hematopoietic, multivitamin, iron supplementation as supportive therapy, and doxycycline antibiotic as causative therapy. The wolf showed decreased salivary excretion and ate 4 h after fluid therapy, atropine sulfate, and hematopoietic administration. The wolf improved their condition through increased appetite and became agile after seven days of treatment. The wolf was declared clinically cured after two weeks of doxycycline treatment.
Co-Authors Anak Agung Ananda Septiarini Anak Agung Gde Arjana Anak Agung Sagung Indraswari Anak Agung Sagung Kendran Anjelia Martina Dewi Bianca Violanda Junus Brahma Tusta Bhirawa Chandra Yowani chyntia nirmalasari mantrawan Fedik Abdul Rantam Franki Remi Andung I Gede Agus Eva Prawira Adinata I Gede Erik Juliarta I Gede Oka Budiawan I Gede Semarabawa I Gede Soma I Gusti Agung Ayu Suartini I Gusti Ketut Suarjana I Gusti Ngurah Agung Antaprapta I K. Berata I Ketut Suatha I Komang Gde Bendesa I Made Kardena I Made Wima Cahyadi I Md Chandra Arya PW I Nengah Anom Adi Nugraha Sibang I Nengah Kerta Besung I Nyoman Suarsana I Nyoman Suartha I P. Suastika I Putu Cahyadi Putra, I Putu Cahyadi I Putu Indra Parmayoga I Putu Juli Sukariada I Putu Sampurna I Putu Suastika I W BUDIARSA SUYASA I Wayan Gunawan I Wayan Masa Tenaya I Wayan Piraksa I Wayan Suardana I Wayan Sudira Ida Ayu Pasti Apsari Ida Bagus Komang Ardana Ida Bagus Ngurah Swacita IK Sudibia IM Suyana Utama IW. Piraksa K.K Agustina Ketut Tono Pasek Gelgel Komang Ariya Hendrayana Komang Yogie Suryana Putra Luh Gde Sri Surya Heryani Luh Kadek Nanda Laksmi M Windhu M. Oenas Adinugroho Mergayanti Yudanta Eka Putri Muh Amiruddin N. Suparta N.N. Suryani Ni Kadek Rahayu Swari Ni Komang Dian Sri Sujani Ni Luh Eka Setiasih Ni Luh Putu Sriyani Ni Luh Putu Wiardani Astuti Ni Luh Sri Sundari Rahayu Ni Luh Watiniasih Ni Made Riska Adnyani Ni Made Riska Adnyani Ni Nyoman Citra Susilawati Ni Nyoman Tri Pujiastari Ni Nyoman Werdi Susari Ni Putu Dewi Setia Sari Ni Wayan Ayu Rukmini Ni Wayan Ayu Rukmini Ni Wayan Suryanadi Ni Wayan Tatik Inggriati Nyoman Sadra Dharmawan Oktavyan Loys Mami P Sampurna P. Suastika Putu Agus Trisna Kusuma Antara Putu Ayu Santika Putu Dian Purnama Putra Putu Henrywaesa Sudipa Putu Satya Dwipartha Putu Suastika Rasdianah Rasdianah Reny Navtalia Sinlae Sri Milfa Tri Ulfah Arema Yanti Widodo Cipto Subagyo Widyasanti, Ni Wayan Helpina Winda Ara Yulisa