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All Journal HAYATI Journal of Biosciences ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Jurnal Pengolahan dan Bioteknologi Hasil Perikanan Journal of Marine Research Indonesian Journal of Biotechnology Jurnal Kelautan Tropis Script Journal: Journal of Linguistic and English Teaching Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Triadik Diksa : Jurnal Pendidikan Bahasa dan Sastra Indonesia Jurnal Ilmiah KORPUS Journal of English Education and Teaching (JEET) Journal of Microbial Systematics and Biotechnology International Journal of Marine Engineering Innovation and Research Mendapo: Journal of Administrative Law ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua Society: Jurnal Pengabdian Masyarakat Demokrasi: Jurnal Ilmu Pemerintahan
Agus Trianto
Department Of Marine Science, Faculty Of Fisheries And Marine Science, Diponegoro University, St. Prof. Soedarto, SH., Tembalang, Semarang 50269, Central Java, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Physics Social Sciences Transportation Other

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Journal : Journal of Microbial Systematics and Biotechnology

 1 
Stability of Phycocyanin Extracted from Spirulina maxima in different pH from Indoor and Semi-outdoor Cultivation Dwi Susilaningsih; Bimo Saskiaoktavian; Ali Djunaedi; Agus Trianto
Journal of Microbial Systematics and Biotechnology Vol 2, No 1 (2020): June 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i1.33

Abstract

Spirulina maxima is a blue-green microalga that rich in pigment. The pigments in S. maxima grouped into primary pigment (Chlorophyll a) and accessory pigments (carotenoid & phycobiliprotein). Phycocyanin is an accessory pigment that belongs to phycobiliprotein, blue colored, and can be used as natural food coloring and drugs. Phycocyanin has activities as antibacterial, antioxidant, anti-inflammatory, antihyperalgesic, and many more. Because of that, phycocyanin usually used in the pharmaceutical industry. However, phycocyanin is a protein that unstable under lights, high temperature, and pH in the storage. This study aims to obtain information about the effect of pH on the stability of phycocyanin extracted from S. maxima that cultivated in indoor and semi-outdoor. The steps are cultivation, extraction using different solutions to get blue pigment phycocyanin, and stability test. Phycocyanin was dissolved in a buffer solution at pH 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, and 7.0 for 7 days. Color observations based on visualization and concentration measurements are carried out every day to see changes in phycocyanin. Growth in indoor cultivation with 24 hours light is faster than semi-outdoor a that uses only sunlight. Cultivation gets the optical density value 0,6 at day 20 with indoor cultivation and day 34 with semi-outdoor cultivation. Results show that phycocyanin can be extracted using a buffer phosphate solution. The stability of the pigments can be seen from the color changes and relative concentration using a spectrophotometer. Phycocyanin shows stable in the storage of pH 4 until pH 5.5. The highest relative concentration (CR) was shown in pH 5.
Co-Authors Agus Sabdono Akhmad Tri Prasetyo Aldi Nuary Nuary Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Ridlo Amanda, Leony Chika Ambariyanto Ambariyanto , Ambariyanto Ambariyanto Andi Afriandi Andi Afriandi Andika, Reta Anggeraeni, Dwi Anicia Hurtado Aninditia Sabdaningsih Apriliani, Seka Indah ardiansyah, vendra Ariesta, Ria Arono Arono Ayu Charismawaty Ayu, Trifajriah Lutea Azhar, Nuril Azis Rifai Aziz Rifai Bahry, Muhammad Syaifudien Bayu Utomo Bimo Saskiaoktavian Carlos, Juan Catur Wulandari Christel Marty Dafa, Yori Hayyan Delianis Pringgenies Desi Anggraini Destio . Desy Wulan Triningsih Diah Permata Wijayanti Diah Permata Wijayanti Diana, Cris Dwi Haryanti Dwi Haryo Ismunarti Dwi Lestari Widya Ningsih Dwi Susilaningsih Dwijayanti, Widya Putri Elfanando Rizky Juliantara Sucipto Elis Indrayanti Endang Supriyantini Endra Wijaya Ervia Yudiati Farrastasya Muflihul Azzami Fatra, Qalbita Fran Ciputra Tambunan Frans Alexander Nainggolan Gita Wismayanti Gumono Gumono, Gumono Hamzah, Syukri Haqqu, Alir Adn Humairah Arifia Sabiladiyni Irene Ulsadriatny Irwani Irwani Isai Yusidharta Ita Puspitasari Ita Widowati Jumarta, Rofi Jusup Supriyanto Jusup Supriyanto Kristi, Roselina Nadya Larasati, Stefanie Jessica Henny Lestari, Martini Tria Lilik Maslukah M. Arifin Ma'ruf, Widodo Farid Mada Triandala Sibero Mada Triandala Sibero Maestro Munru Mahadika Fanindhita Sany McCauley, Erin Muhammad Arifin Muhammad S. Bahry Muhammad Syaifudien Bahry Muhammad Syaifudien Bahry Munasik Munasik Munru, Maestro Nafri Yanti Nathalie Bourgougnon Ni Komang Tri Utami Nining Nursalim Nugraha Ridho Ikhsani, Nugraha Ridho Nugroho Agus D Nugroho Hendartono Nugroho Hendartono Nur Taufiq-Spj Ocky Karna Radjasa Popi IL Ayer Purwadi, Agus Joko Puspitasari, Ita Putri, Dear Berliana Rachmat Afriyanto Raden Ario Radisya N Nissa Rahmaini, Putri Raja Aditia Sahala Siagian Retno Hartati Retno Murwani Rexie Magdugo Rizky Rifatma Jezzi Rohimat Rohimat Rosida, Nina Rudhi Pribadi Rudhi Pribadi Sabiladiyni, Humairah Arifia Sakti Imam Muchlisin Sari, Dian Lupita Sari, Else Puspita Sarwono, Sarwit Sekar Widyaningsih Sembiring, Yudhawira Bhaskara Septhy Kusuma Radjasa Shari, Vio Deswitha Shofiatun Nimah Sri Sedjati Subagiyo Subagiyo Sucipto, Elfanando Rizky Juliantara Susetyo Susetyo Tambunan, Fran Ciputra Taufiq-Spj, Nur Trifajriah Lutea Ayu Twenty Agustiola, Agnes Ujiana, Rica Utomo, Padi Widianingsih Widianingsih Wiratno Wiratno Wisman Wismayanti, Gita Wittriansyah, Khoeruddin Yonnawati, Yonnawati Yudhawira Bhaskara Sembiring Yulistio, Didi