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Uji Aktivitas Antioksidan Gummy Candy Ekstrak Daun Sirih Cina (Peperomia pellucida L. Kunth) dengan Metode DPPH Ni Putu Putri Cahya Anggreni; Ni Putu Refina Dharma Yanti; Kadek Ayu Puspa Pratiwi; Ni Nyoman Wahyu Udayani
Indonesian Journal of Pharmaceutical Education Vol 3, No 3 (2023): September-Desember 2023
Publisher : Jurusan Farmasi Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/ijpe.v3i3.22117

Abstract

The body's defense that functions to neutralize free radicals is antioxidants. The Peperomia pellucida is a medicinal plant that has secondary metabolite compounds such as alkaloids, flavonoids, saponins, tannins and steroids. Antioxidants in Chinese betel plants are associated with the prevention and treatment of several diseases. The aim of this research was to determine the antioxidant activity contained in Peperomia pellucida and Peperomia pellucida gummy candy preparations. This research uses the main ingredient of peperomia pellucida obtained from the Jembrana district, Bali province. The total weight of the extract obtained was 30 grams. Then the materials used are peperomia pellucida extract gummy candy, DPPH powder, 96% ethanol, chloroform, ammonia, H2SO4, Dragendorf reagent, concentrated HCL, magnesium powder, CH3COOH, iron (III) chloride, distilled water. This research uses the DPPH method in antioxidant testing and phytochemical screening testing in testing peperomia pellucida extract. The results of the phytochemical screening test for peperomia pellucida extract showed positive results in the alkaloid, flavonoid, steroid, saponin and tannin tests. In the results of the antioxidant activity test using the DPPH method, the antioxidant activity results of Chinese betel extract showed an IC50 value of 564,062 ppm. Gummy candy peperomia pellucida extract 100mg/kgBB showed an IC50 value of 904,342 ppm. In gummy candy, peperomia pellucida extract 200mg/kgBB showed an IC50 value of 381.205 ppm. And gummy candy peperomia pellucida extract 400mg/kgBB showed an IC50 value of 347,227 ppm. Based on the research results obtained, it can be said that the IC50 value is very weak. This can be seen from the IC50 value 200 ppm. Based on the results of research, from Chinese betel extract, gummy candy Chinese betel extract doses of 100mg/kgBB, 200mg/kgBB, and 400mg/kgBB which contain the best antioxidants, which are found in gummy candy formula 200 mg/kgBB with results of 347,227 ppm.
Aktivitas Kombinasi Krim Daun Pare (Momordica charantia L) dan Kulit Jeruk (Citrus nobilis) dalam Penyembuhan Luka Bakar I Made Agus Sunadi Putra; Made Dwik Lestari; Ni Nyoman Wahyu Udayani; Ketut Agus Adrianta; Nyoman Budiartha Siada
Journal Syifa Sciences and Clinical Research Vol 5, No 2 (2023): Volume 5 Edisi 2 2023
Publisher : State University of Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/jsscr.v5i2.21269

Abstract

Burns are injuries to the skin caused by heat, fire, hot steam, exposure to chemicals, solar radiation, or electric shock. Burns need to be treated immediately because they can cause infection in the skin. Healing burns can be done by utilizing several types of existing plants, namely bitter melon leaves and orange peels which are known to be able to help heal burns because they contain secondary metabolites such as flavonoids, tannins, saponins, steroids, alkaloids, and terpenoids which can be used for wound healing. The purpose of this study was to determine the effect of a combination of bitter melon leaf extract cream and orange peel as healing burns in white rats. This research is a quantitative study using laboratory experimental methods with a post test only control group design. In this study, male white rats were made with wounds on the back skin. Using 20 white rats divided into 5 different treatment groups. The negative control was given a cream base, the positive control was given Burnazin cream, F1 3% : 5%, F2 ie 4% : 4%, F3 ie 5% : 3%. The wound was smeared with preparations according to group division and carried out for 14 days. Based on the results of observations of the effect of a combination of bitter melon leaf extract and orange peel cream at F1 concentrations with a concentration ratio of 3%: 5%, it has a faster healing effect on burn area. Based on the results of the Kruskal wallis test on the healing of second degree burns in male white rats, there was a significant difference. The p value or sig is 0.014, so it can be concluded that there is a significant difference between the healing areas of burns in white rats.
Literature study: Activity of white turmeric (Curcuma zedoaria) as an anti-inflammatory Ni Nyoman Wahyu Udayani; Ni Nyoman Ayu Puspita Sari; I Made Agus Sunadi Putra; I Putu Tangkas Suwantara; Maria Malida Vernandes Sasadara; Ni Putu Lilis Adnyani
Journal of Midwifery and Nursing Vol. 5 No. 3 (2023): September: Health Science
Publisher : Institute Of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/jmn.v5i3.4092

Abstract

Inflammation is a response given by the body to protect the body against cellular damage, characterized by swelling, redness accompanied by a sensation of heat and pain. Long term use of NSAIDs (Non Steroid Anti Inflammatory Drugs) has side effects such as ulcers, stomach upset, bleeding, nausea, disturbances in the kidneys and the cardiovascular system. White turmeric (Curcuma zedoaria) is a plant that can be used as an anti-inflammatory. This study aims to determine the anti-inflammatory activity of White Turmeric (Curcuma zedoaria) through a literature review in the form of national and international articles or journals. The results of a study of 8 articles that met the inclusion requirements showed that White Turmeric has anti-inflammatory activity. The content of curcumin, essential oils and flavonoids in white turmeric has similarities with NSAIDs, namely by inhibiting the formation of inflammatory mediators.
Uji Aktivitas Antioksidan Ekstrak Etanol Sirih Cina (Pepperomia pellucida) dengan Metode DPPH (1,1-diphenyl-2-picrylhydrazyl) Ni Putu Refina Dharma Yanti; Ni Putu Putri Cahya Anggreni; Kadek Ayu Puspa Pratiwi; Ni Nyoman Wahyu Udayani; Ketut Agus Adrianta
Indonesian Journal of Pharmaceutical Education Vol 3, No 3 (2023): September-Desember 2023
Publisher : Jurusan Farmasi Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/ijpe.v3i3.22417

Abstract

Free radicals play an important role in pathological processes and tissue damage. The pepperomia pellucida plant is often used as a traditional medicine, known to have antibacterial, anti-inflammatory, hypoglycemic, antifungal, antimicrobial, anticancer, antioxidant, antidiabetic and antihypertensive activity. Excessive free radicals can attack vulnerable compounds such as lipids and proteins which can cause various diseases. The Pepperomia pellucida plant is a plant that contains secondary metabolites such as alkaloids, flavonoids, saponins, tannins and steroids. The aim of this research was to determine the antioxidant activity of Pepperomia pellucida ethanol extract with variations of 70% and 80%. The results of the DPPH (1,1-diphenyl-2-picrylhydrazyl) ethanol extract of Pepperomia pellucida extract in the 70% variation showed IC50 = 24.50981 ppm and the 80% variation showed IC50 = 30.49915 ppm. Based on the research results of the two variations of ethanol, it was found that the best IC50 result was Pepperomia pellucida ethanol extract with a variation of 70% because it had a smaller IC50 result than the 80% ethanol variation, namely with an IC50 figure = 24.50981 ppm. The results of the phytochemical screening of the ethanol extract of pepperomia pellucida indicated positive results from several tests, namely alkaloids, flavonoids, steroids, saponins and tannins.
Formulation and Inhibitory Test of Feminine Cleansing Soap Combination of Young Areca Extract (Areca cathechu L.) and Manjakani Extract (Quercus infectoria) on the Growth of Candida Albicans Ni Nyoman Wahyu Udayani; Ketut Agus Adrianta; I Gede Made Suradnyana; Ni Luh Putu Tris Yoga Cahyani
Gema Lingkungan Kesehatan Vol. 22 No. 2 (2024): Gema Lingkungan Kesehatan
Publisher : Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36568/gelinkes.v22i2.121

Abstract

One of the reproductive health problems that women often experience is vaginal discharge. About 85-95% vaginal discharge is caused by the fungus Candida albicans. To maintain the cleanliness of the female organ system, feminine cleansing soap is usually used. The use of traditional ingredients has smaller side effects and the price is also relatively affordable. Traditional ingredients that can be used are young areca nut (Areca catechu L.) and Manjakani (Quercus infectoria). Young areca nut seeds contain tannins, flavonoids, and saponins, while Manjakani fruit contains tannins and flavonoids which have anti-fungal activity. This study aims to determine the inhibitory power of feminine cleansing soap from areca nut extract (Areca cathechu L.) and Manjakani extract (Quercus infectoria) on the growth of Candida albicans. This research is a purely experimental laboratory which begins with the extraction process. 5 formulations of feminine cleansing soap were prepared, a combination of young areca nut extract (Areca cathechu L.) and Manjakani extract (Quercus infectoria) with a concentration percentage ratio of 5:10, 7.5:7.5, 10:5, 15:0 respectively and 0:15. The resulting preparations were tested for inhibition of the growth of Candida albicans. The results showed that a feminine cleansing soap combination of young areca nut extract (Areca cathechu L.) and Manjakani extract (Quercus infectoria) had an inhibitory effect on the growth of Candida albicans, namely F1, F2, F3, F4 and F5 which had average inhibition respectively of 21.33 ± 0.58 mm, 21.67 ± 0.58 mm, 22 ± 0 mm, 19.67 ± 1.53 mm. Thus, the findings show that all feminine cleansing soap formulations of a combination of young areca nut extract (Areca cathechu L.) and Manjakani fruit extract (Quercus infectoria) have an inhibitory effect on the growth of Candida albicans. Of the five formulations tested, the combination of extracts with a ratio of 0:15 (F5) showed the highest inhibition against Candida albicans with a mean inhibition zone diameter of 22.33 ± 0.58 mm.
Skrining Fitokimia dan Aktivitas Antioksidan Ekstrak Daun Benalu Jeruk (Dendrophthoe glabrescens (Blakely) Barlow) dengan Pelarut n-Heksan dan Etanol Udayani, Ni Nyoman Wahyu; Wiguna, Putu Dony Saputra; Cahyaningsih, Erna; Wardani, I Gusti Agung Ayu Kusuma
Jurnal Ilmiah Medicamento Vol 9 No 2 (2023): Jurnal Ilmiah Medicamento
Publisher : Fakultas Farmasi Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36733/medicamento.v9i2.7136

Abstract

One of the compounds that can be exploited from plants is antioxidants. Antioxidants are compounds that are important in overcoming free radicals in the body. Natural antioxidants are more desirable than synthetic ones because of their minimal side effects. Mistletoe is a parasitic plant that has been used in traditional medicine in Indonesia. This study aims to determine the content of secondary metabolites contained in citrus parasite leaf extracts using non-polar and polar solvents and how their antioxidant activity is. with a ratio of material and solvent 1:10. The screening tested included alkaloids, triterpenoids, steroids, flavonoids, saponins, and tannins. The method used to test the antioxidant activity of the orange parasite leaf extract is the DPPH method as measured by a UV-Vis spectrophotometer. Statistical analysis was carried out using One Way Anova and Post Hoc on the IC50 value of the extract with a 95% level of confidence. From the results of research conducted, the N-Hexane extract of citrus parasite leaves contained secondary metabolites of alkaloids, steroids and flavonoids, and the ethanol extract of orange parasite leaves contained secondary metabolites of alkaloids, steroids, flavonoids, saponins, and tannins. The antioxidant activity of the n-Hexane and ethanol extracts were 92.731 ppm (strong) and 54.490 ppm (strong) respectively.
Efektivitas Krim Ekstrak Daun Erythrina subumbrans (Hassk.) Merr. terhadap Diameter Luka Bakar Derajat IIA Wardani, I Gusti Agung Ayu Kusuma; Udayani, Ni Nyoman Wahyu; Suena, Ni Made Dharma Shantini
Jurnal Ilmiah Medicamento Vol 10 No 1 (2024): Jurnal Ilmiah Medicamento
Publisher : Fakultas Farmasi Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36733/medicamento.v10i1.8198

Abstract

Burn injuries involve tissue damage caused by heat, including exposure to hot steam, fire, radiation, electricity, hot liquids or fats, electrical currents, and chemical compounds. Dadap Serep leaves (Erythrina subumbrans (Hassk) Merr) are among the plants recorded in Usada Bali, known for treating various types of wounds. This research aimed to assess the effectiveness of ethanol extract cream from Dadap Serep leaves in reducing the diameter of second-degree burns in vivo using white rats as test subjects. Twenty-four rats were randomly divided into four treatment groups: K- (negative control), K+ (positive control), P1 (application of Dadap Serep leaf extract cream 20%), and P2 (application of Dadap Serep leaf extract cream 40%). The cream was topically applied twice daily for 14 days. The burn diameter results were analyzed using SPSS 25 software employing the Tukey Test. Post hoc Tukey testing revealed significant differences between the negative control and positive control groups, treatments 1 and 2, with p-values of 0.001, 0.043, and 0.012, respectively. No significant difference was observed between the positive control group and treatment groups 1 and 2, with p-values of 0.268 and 0.596, respectively. The ethanol extract cream from Dadap Serep leaves proved effective in reducing the diameter of second-degree burns on rat skin. The Dadap Serep leaf extract cream at a concentration of 20% was effective in reducing the diameter of second-degree burns on rat skin.
Analysis of Adverse Drug Reactions Combination of Zidovudine, Lamivudine, and Nevirapine Drugs in the Treatment of HIV/AIDS Patients in a Denpasar District Hospital Udayani, Ni Nyoman Wahyu; Dewi, Putu Intan Candra
Budapest International Research and Critics Institute-Journal (BIRCI-Journal) Vol 4, No 4 (2021): Budapest International Research and Critics Institute November
Publisher : Budapest International Research and Critics University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33258/birci.v4i4.2858

Abstract

HIV/AIDS is a disease caused by infection with the Human Immunodeficiency Virus (HIV). Antiretroviral therapy is used for treatment. The three most commonly used combination antiretroviral regimens are zidovudine, lamivudine, and nevirapine. The problem that arises from the use of antiretroviral drugs is the emergence of adverse drug reactions (ADR). The purpose of this study was to identify and analyze the incidence of adverse drug reactions from the use of combination antiretroviral drugs in the treatment of HIV/AIDS patients in a Denpasar district hospital. This study is a descriptive study with prospective data collection on the patient's medical record. The sampling technique in this study uses a non-probability technique (Purposive Sampling). This study involved 102 patients. The results obtained were descriptive analysis for patient demographic data, duration of therapy and adverse drug reactions that occurred. The results showed that there was an adverse drug reaction in 50% of patients who used a combination of antiretroviral drugs zidovudine, lamivudine and nevirapine. The longest duration of ARV therapy in one of the hospitals in Denpasar Regency was the age > more than 2 years to 4 years, which was 33.3%. The types of ADR that occurred were nausea/vomiting (25 events), skin rash (23 events), headache (11 events), lethargy/fatigue (6 events), fever (6 events), muscle pain (5 events), diarrhea (4 events), neuropathy (2 events), anemia (2 events), and the least is nail hyperpigmentation (1 event).
Phytochemical and Pharmacological Activities of Three Colors Fruit (Eugenia Uniflora L) As Antidiabetes and Antibacteries Santoso, Puguh; Udayani, Ni Nyoman Wahyu; Putra, I Made Agus Sunadi; Yuwono, Moch.
Budapest International Research and Critics Institute-Journal (BIRCI-Journal) Vol 4, No 4 (2021): Budapest International Research and Critics Institute November
Publisher : Budapest International Research and Critics University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33258/birci.v4i4.2861

Abstract

Eugenia uniflora with local name Indonesia   Dewandaru is a plant of the family Myrtaceae. It has green, orange, red, to blackish purple fruits which is widespread in South America, Asia, Africa and Australia. E. uniflora can be used as traditional medicine. The fruits is parts of plants that are widely used in various field nutrition, cosmetic and health, . The active secondary contained in this fuits were applied in anti inflammatory, anti diabetic, adstringent and antioxidant.   Several active compounds found in fruits such a carotene esensial oil and polyphenols have been reported to have some biological activities such as antioxidant antidiabetic and antibacterial. The level maturity of fruits level is related to the contend of secondary metabolites such a fenoloic, carotene esential oils.  The color of E. uniflora fruit is related to the active phytochemical content and antioxidant activity. Three colors of E. uniflora fruit contain beta carotene and phenolic compounds, essential oil, the highest content of phenolic compounds and antioxidants is found in purple fruit This review provides insight into the effort of discovery and development of pharmaceutical agents derived from fruits of Dewandaru (Eugenia uniflora) plants to  make product.
Penetapan Kadar Senyawa Fitokimia (Alkaloid, Flavonoid dan Tanin) pada Ekstrak Etanol Rimpang Kunyit Hitam (Curcuma Caesia Roxb.) Wahyu Udayani, Ni Nyoman; Mega Ratnasari, Ni Luh Ayu; Yustari Nida, I Dewa Ayu Anom
Jurnal Pendidikan Tambusai Vol. 6 No. 1 (2022): 2022
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai, Riau, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jptam.v6i1.3256

Abstract

Indonesia merupakan Negara tropis yang memiliki keanekaragaman hayati bahan alam. Jumlah tumbuhan berkhasiat obat di Indonesia diperkirakan sekitar 1.260 jenis tumbuhan. Setiap tumbuhan memiliki kandungan metabolit sekunder yang spesifik atau berbeda disetiap tumbuhan dengan fungsi yang juga berbeda-beda. Curcuma caesia Roxb. atau yang dikenal dengan nama kunyit hitam merupakan tanaman yang memiliki banyak khasiat. Penelitian ini bertujuan untuk mengetahui kadar flavonoid, tannin dan alkaloid yang terdapat dalam rimpang kunyit hitam. Preparasi sampel rimpang kunyit hitam dilakukan dengan teknik ekstraksi maserasi dengan pelarut etanol 80%. Analisis kadar tannin dan flavonoid ditentukan dengan Spektrofotometri UV-Visibel pada panjang gelombang (?) 415 untuk flavonoid dan panjang gelombang (?)725 nm. Sedangkan analisis kadar alkaloid menggunakan metode gravimetri. Hasil analisis menunjukkan kadar flavonoid rimpang kunyit hitam sebesar yaitu 2775,65 mg/100 gram; tannin sebesar 2714,75 mg/100 gram dan alkaloid sebesar 1470,588235 mg/100 gram.
Co-Authors Adnyani, Putu Mesi Dian Adrianta, I Ketut Agus Agung Ari Chandra Wibawa Agung Ari Chandra Wibawa Agustin, Ni Kadek Jessica Agustini, Ni Putu Dewi Anggreni, Ayu Putu Puspa Anita Dewi, Ni Luh Kade Arman Apriani, Rista Ciptawati, Ni Wayan Cokorda Javandira Debby Juliadi Dewi, Ni Kadek Dhea Cipta Dewi, Putu Intan Candra Dwipayanti, Ni Kadek Sari Erna Cahyaningsih Febryna Dharma Yanti, Ni Luh Putu Fitria Megawati Fitria Megawati Fredinan Yulianda Fridayana, Ni Luh Gede Erica Herleeyana Meriyani Herleeyana Meriyani I Dewa Ayu Anom Yustari Nida I Gede Made Suradnyana I Gede Yodhi Trismawan I Gusti Agung Ayu Kusuma Wardhani I Gusti Ayu Arya Ditha Suari I Ketut Sumantra I Made Agus Sunadi Putra I Made Agus Sunadi Putra I Made Agus Sunadi Putra I Made Agus Sunadi Putra I Nyoman Gede Tri Sutrisna I Putu Pasek Jaya Separsa I Putu Tangkas Suwantara I Putu Werda Bisama Ida Ayu Gede Syntya Mahayani IGAA Kusuma Wardani Kadek Ayu Puspa Pratiwi Ketut Agus Adrianta Ketut Agus Adrianta Ketut Agus Adrianta Ketut Agus Adrianta Ketut Agus Adrianta Ketut Agus Adrianta Ketut Agus Adrianta Laksmi, Luh Putri Dianti Lestari, Ketut Sita Citra Lilis Adnyani Ni Putu Luh Putu Febryana L Lusi Puspitasari, Ni Wayan Made Dwik Lestari Maharani, Ade Dwi Karisma Mahardika, I Made Agus Mahendra, I Putu Aldy Manik Suka Dewi Yani Ni Komang Maria Daniela Tupa Hokor Maria Malida Vernandes Sasadara Mayun Anak Agung Istri Mega Ratnasari, Ni Luh Ayu Megawati, Fitri Megawati, Fitria Meika, Ni Putu Trisna Mendra, Ni Nyoman Yudianti Meriyani, Herleeyana Nggaba, Ellenoire Rambu Kabeba Hammu Ni Komang Enny Wahyuni Ni Luh Ayu Mega Ratnasari Ni Luh Kade Arman Anita Dewi Ni Luh Kade Arman Anita Dewi Ni Luh Putu Dewi Purnama Sari Ni Luh Putu Sulasmini Ni Luh Putu Tris Yoga Cahyani Ni Made Dharma Shantini Suena Ni Made Dwi Mara Widyani Nayaka Ni Made Sukma Sanjiwani Ni Nyoman Ayu Puspita Sari Ni Putu Dewi Agustini Ni Putu Leona Yorita Dewi Ni Putu Lilis Adnyani Ni Putu Lilis Adnyani Ni Putu Putri Cahya Anggreni Ni Putu Refina Dharma Yanti Ni Putu Sintia Rahayu Ni Wayan Ciptawati Ni Wayan Riastini Nindya Kartika, Dewa Ayu Kintan Nugraha, Nyoman Dhamapada Nyoman Budiartha Siada Pendet, Ni Made Diah Pusparini Puguh Santoso Puguh Santoso puguh santoso, puguh Putra Hilmi Prayitno Putra, I Made Agus Sunadi Putri, Ni Komang Ary Agustina Berliana Putu Era Sandhi K.Yuda Putu Era Sandhi Kusuma Yuda Putu Mesi Dian Adnyani Riastini, Ni Wayan Sasadara, Maria Malida Vernandes Siluh Putu Astini Sugijanto, Milyadi Sunadi Putra I Made Agus Wardani, I Gusti Agung Ayu Kusuma Widiasriani, Ida Ayu Putu Wiguna, I Putu Bagus Artha Wiguna, Putu Dony Saputra Woro Enjang Santi Prihatin Wulandari, Ni Kadek Ganis Yowani, Anak Agung Sagung Iswarani Yustari Nida, I Dewa Ayu Anom Yuwono, Moch.