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Efektivitas Stick Herbal Usada Bali sebagai Antioksidan dan Anti-Inflamasi pada Mencit Putih Jantan yang Diinduksi Karagenan Ketut Agus Adrianta; I Putu Werda Bisama; Ni Nyoman Wahyu Udayani; Ni Made Dharma Shantini Suena; Agung Ari Chandra Wibawa
JPSCR: Journal of Pharmaceutical Science and Clinical Research Vol 9, No 1 (2024)
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jpscr.v9i1.71522

Abstract

: Inflamasi disebabkan oleh terlepasnya mediator pro inflamasi di dalam tubuh, seperti TNF-α, caspase-3, dan prostaglandin. Tujuan penelitian ini adalah untuk mengetahui aktivitas antioksidan dan antiinflamasi dari stick herbal Usada Bali yang mengandung rimpang kencur, bunga cengkeh, dan daun kelor pada mencit putih jantan yang diinduksi karagenan. Penelitian dilakukan dengan rancangan penelitian eksprimental laboratorium, dimana dalam pengujian antioksidan digunakan penelitian in-vtiro DPPH dengan instrument spektrofotometri UV-Vis, sedangkan pengujian antiinflamasi berupa penelitian in-vivo dengan rancangan randomize pre test and postest with control group design dan menggunakan hewan coba dengan rincian pembagian kelompok sebagai berikut: Kelompok 1 (P1) adalah kelompok perlakuan plasebo, kelompok 2 (P2) adalah kelompok perlakuan yang diberikan natrium diklofenak, kelompok 3 (P3) adalah kelompok perlakuan yang diberikan Stick Herbal Usada Bali. Pengujian antioksidan memberikan hasil bahwa sampel memiliki nilai IC50 82,285 µg/mL dengan kategori (kuat) dan nilai Antioxidant Activity Index 0,469 dengan katagori (sedang). Pengujian antiinflamasi berdasarkan analisis SPSS menunjukkan bahwa terdapat perbedaan bermakna antara kelompok kontrol negatif dengan kelompok perlakuan Stick Herbal Usada Bali dengan nilai p=0,000 (p<0,05), serta tidak terdapat perbedaan bermakna antara kelompok positif dengan kelompok perlakuan Stick Herbal Usada Bali p=0,222 (p>0,05). Efektivitas antioksidan dan antiinflamasi Stick Herbal Usada Bali dipengaruhi oleh senyawa yang terkandung di dalamnya yaitu flavonoid dan saponin yang diketahui memiliki khasiat sebagai antioksidan dan antiinflamasi. Sehingga, dapat disimpulkan bahwa Stick Herbal Usada Bali berpotensi sebagai alternatif terapi antiinflamasi dan memiliki efektivitas sebagai antioksidan.
Potential of turmeric cream (curcuma caesia roxb.) extract in incision wounds Udayani, Ni Nyoman Wahyu; Adrianta, I Ketut Agus; Wiguna, I Putu Bagus Artha; Yowani, Anak Agung Sagung Iswarani
MEDISAINS Early Release
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/medisains.v0i0.23731

Abstract

Background: Incisional wounds are the most common wounds in the community. Improper handling of incision wounds can worsen wounds and cause infection. The content of secondary metabolites in black turmeric rhizomes can act as anti-inflammatory and antimicrobial in the healing process of incision wounds. However, its effectiveness in healing incision wounds has not been widely studied, so further research is needed.Purpose: This study was conducted to determine the potential of black turmeric cream as an herbal medicine to heal incision wounds.Methods: This study was an invivo experimental research study with a randomized post-test control group design. The samples used were 20 male rats that were made to have a 2 cm incision wound on the back area, consisting of a negative control, a positive control, and three black turmeric extract groups (5%, 10%, and 15%). The data obtained were analyzed by an ANOVA (Analysis of Variance) parametric statistical test.Result: The results showed that the highest decrease in incision wound length was found in the black turmeric rhizome cream Formula III group and the lowest in the negative control group. On day 14, the incision wound area of the Formula III black turmeric rhizome cream group had closed or healed (0.00 cm), while the negative control group had the slowest incision wound closure (0.39 cm).Conclusion: Black turmeric rhizome cream can heal and prevent infection of the incision wound. This shows the potential of black turmeric rhizome as an anti-inflammatory and antimicrobial.
Potential of turmeric cream (curcuma caesia roxb.) extract in incision wounds Udayani, Ni Nyoman Wahyu; Adrianta, I Ketut Agus; Wiguna, I Putu Bagus Artha; Yowani, Anak Agung Sagung Iswarani
MEDISAINS: Jurnal Ilmiah Ilmu-Ilmu Kesehatan Early Release
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/medisains.v0i0.23731

Abstract

Background: Incisional wounds are the most common wounds in the community. Improper handling of incision wounds can worsen wounds and cause infection. The content of secondary metabolites in black turmeric rhizomes can act as anti-inflammatory and antimicrobial in the healing process of incision wounds. However, its effectiveness in healing incision wounds has not been widely studied, so further research is needed.Purpose: This study was conducted to determine the potential of black turmeric cream as an herbal medicine to heal incision wounds.Methods: This study was an invivo experimental research study with a randomized post-test control group design. The samples used were 20 male rats that were made to have a 2 cm incision wound on the back area, consisting of a negative control, a positive control, and three black turmeric extract groups (5%, 10%, and 15%). The data obtained were analyzed by an ANOVA (Analysis of Variance) parametric statistical test.Result: The results showed that the highest decrease in incision wound length was found in the black turmeric rhizome cream Formula III group and the lowest in the negative control group. On day 14, the incision wound area of the Formula III black turmeric rhizome cream group had closed or healed (0.00 cm), while the negative control group had the slowest incision wound closure (0.39 cm).Conclusion: Black turmeric rhizome cream can heal and prevent infection of the incision wound. This shows the potential of black turmeric rhizome as an anti-inflammatory and antimicrobial.
Aktivitas Antioksidan Ekstrak Etanol Kulit Biji dan Buah Bakau (Xylocarpus granatum J.Koenig) dengan Metode DPPH dan FRAP Wardani, I Gusti Agung Ayu Kusuma; Adrianta, Ketut Agus; Udayani, Ni Nyoman Wahyu; Fridayana, Ni Luh Gede Erica; Mendra, Ni Nyoman Yudianti; Suena, Ni Made Dharma Shantini
Jurnal Ilmiah Medicamento Vol 11 No 1 (2025): Jurnal Ilmiah Medicamento
Publisher : Fakultas Farmasi Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36733/medicamento.v11i1.9426

Abstract

Free radical compounds are molecules that possess reactive and unstable properties, which can damage biomolecules by disrupting the integrity of DNA, proteins and lipids. This condition can trigger oxidative stress, which can result in degenerative diseases. Humans require antioxidant intake from external sources when their own endogenous antioxidants are insufficient to counteract free radicals. Previous studies have demonstrated the high antioxidant activity of mangrove seeds. However, the antioxidant potential of mangrove seed coats and fruits remains underexplored, particularly when assessed using the DPPH and FRAP methods. This study aims to evaluate the antioxidant activity of seed coat and fruit extracts of Xylocarpus granatum J. Koenig using the DPPH and FRAP methods. This research is quantitative research with laboratory experimental approach. Antioxidant activity testing using DPPH and FRAP methods. Gallic acid was used as a comparison standard for antioxidant activity. The results obtained from the antioxidant activity testing of the ethanol extracts of seed shell, fruit and gallic acid were IC50 values of 10.83 mg/mL, 4.91 mg/mL and 0,793 mg/mL (DPPH) and RP50 of 4,455.63 mg/mL, 15,556.32 mg/mL and 34.04 mg/mL (FRAP). It is acknowledged that discrepancies in testing methodologies can influence the antioxidant activity of each extract, contingent on the nature of the free radical, the solvent, steric accessibility, and the pH of the medium.
Pengaruh Pemberian Ekstrak Kunyit Putih (Curcuma zedoaria Rosc.) terhadap Kadar Malondialdehid (MDA) Tikus Putih (Rattus norvegicus) yang Terpapar Asap Rokok Putra, I Made Agus Sunadi; Suwantara, I Putu Tangkas; Sasadara, Maria Malida Vernandes; Udayani, Ni Nyoman Wahyu
Jurnal Ilmiah Medicamento Vol 11 No 1 (2025): Jurnal Ilmiah Medicamento
Publisher : Fakultas Farmasi Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36733/medicamento.v11i1.10793

Abstract

Cigarette smoke generates free radicals that induce oxidative stress and damage various biomolecules in the body. An increase in malondialdehyde (MDA) levels is a key marker of oxidative stress. The body’s antioxidant mechanisms rely on compounds capable of neutralizing free radicals. White turmeric (Curcuma zedoaria Rosc.) contains antioxidant compounds predicted to prevent oxidative damage, though its in vivo potential remains to be proven. This study aimed to evaluate the effect of white turmeric ethanol extract on MDA levels in male Wistar rats (Rattus novergicus) exposed to cigarette smoke. A post-test only control group design was employed. The rats were divided into five groups: healthy control (P1), smoke-exposed control (P2), extract-treated groups receiving 100 mg/kgBW (P3) and 200 mg/kgBW (P4), and a positive control group receiving vitamin C (P5). Cigarette smoke exposure was administered for 14 days, and blood samples were collected from the orbital vein on day 15. MDA levels were measured using the ELISA method. The mean MDA levels (mmol/ml) for P1 to P5 were 0.118, 0.121, 0.094, 0.059, and 0.089, respectively. ANOVA and post hoc LSD tests (α = 0.05) showed a significant difference between P2 and P4 (p < 0.05), while no significant differences were found between P3, P4, and P5. In conclusion, white turmeric extract at 200 mg/kgBW significantly reduced MDA levels and demonstrated antioxidant activity comparable to that of vitamin C.
PENGENALAN VIRUS CACAR MONYET DAN CARA PENCEGAHAN DENGAN PRODUK HERBAL KEPADA MASYARAKAT WILAYAH BANJAR SAMA KELURAHAN PEDUNGAN DENPASAR SELATAN Wahyu Udayani, Ni Nyoman; Santoso, Puguh; Laksmi, Luh Putri Dianti; Agustin, Ni Kadek Jessica
Jurnal Abditani Vol. 8 No. 1 (2025): April
Publisher : FAKULTAS PERTANIAN UNIVERSITAS ALKHAIRAAT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31970/abditani.v8i1.349

Abstract

Cacar monyet, juga dikenal sebagai monkeypox, disebabkan oleh infeksi virus yang disebabkan oleh genus orthopoxvirus. Di Bali sendiri, belum ada kasus yang terkonfirmasi dari virus cacar monyet, tetapi perlu ada himbauan tentang virus dan cara mencegahnya. Salah satu cara untuk menghindari virus cacar monyet adalah dengan menggunakan tanaman herbal. Kegiatan ini menggunakan penyuluhan langsung melalui buku saku yang dibagikan kepada masyarakat di wilayah Banjar Sama, Kelurahan Pedungan. Untuk menilai pemahaman masyarakat terkait virus cacar monyet (monkeypox) yakni dengan dibagikannya kuisoner berupa pre-test dan post-test kepada 30 responden. Setelah dilakukannya pengolahan data dengan SPPS dapat dilihat adanya peningkatan pengetahuan terlihat dari Uji Wilcoxon nilai Asmp. Sig (2-tailed) kurang dari 0,05 menunjukkan bahwa perbedaan yang terlihat dari hasil nilai pre-test dan post-test berbeda bermakna atau signifikan hasil pre-test dam pos-test responden. Hasil yang dicapai yaitu masyarakat wilayah Banjar Sama, Kelurahan Pedungan sudah memahami tentang virus cacar monyet dan berhasil terealisasikan 100 %.
Ethnobotanical Insights and Quantitative Evaluation of Medicinal Plant Utilization in Traditional Cosmetic Practices: A Community-Centered Study Dewi, Ni Luh Kade Arman Anita; Pendet, Ni Made Diah Pusparini; Apriani, Rista; Megawati, Fitri; Udayani, Ni Nyoman Wahyu; Sugijanto, Milyadi; Agustini, Ni Putu Dewi; Javandira, Cokorda
Bali Medical and Wellness Journal Vol. 2 No. 1 (2025): Bali Medical and Wellness Journal
Publisher : PT BMW Journal Sejahtera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71341/bmwj.v2i1.34

Abstract

Background: Indigenous knowledge about medicinal plants used in cosmetics is an important cultural heritage that needs scientific documentation and preservation. In Terunyan Village, Bali, Indonesia, traditional ethnobotanical practices have been passed down orally through generations. Objective: This study aims to systematically record, identify, and analyze the plant species used by the community for natural cosmetic purposes, and to assess their cultural significance using ethnobotanical indices. Methods: An ethnobotanical survey was carried out using semi-structured interviews with 328 informants selected through purposive sampling. Plant specimens were collected, identified, and voucher samples stored at the Herbarium Hortus Botanicus Baliense (THBB). Quantitative analysis used Use Value (UV) and Fidelity Level (FL) to measure cultural importance and consensus. Results: A total of 24 plant species from 18 plant families were documented for cosmetic use. The most frequently cited species were Aloe vera (UV = 0.180), Cocos nucifera (UV = 0.174), and Solanum lycopersicum (UV = 0.169). Leaves were the most used plant parts (40.85%), and pounding was the main processing method (20.43%). Six species had a fidelity level of 100%, indicating strong cultural agreement. The most common applications were for hair care (35.2%), skin moisturizing (28.7%), and skin brightening (24.1%). Conclusion: This research provides the first detailed documentation of traditional cosmetic plant use in Terunyan Village. It highlights significant ethnobotanical diversity and strong cultural consensus for key species. These findings support the preservation of indigenous knowledge and lay the groundwork for sustainable development of natural cosmetic products.
Skrining Fitokimiadan Uji Aktivitas Ekstrak Buah Dewandaru (Eugeniauniflora .L) pada Hewan Coba yang Di Induksi Aloksan Santoso, Puguh; Nggaba, Ellenoire Rambu Kabeba Hammu; Udayani, Ni Nyoman Wahyu; Adrianta, Ketut Agus; Putra, I Made Agus Sunadi
Jurnal Locus Penelitian dan Pengabdian Vol. 4 No. 7 (2025): JURNAL LOCUS: Penelitian dan Pengabdian
Publisher : Riviera Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58344/locus.v4i7.4681

Abstract

Diabetes melitus merupakan gangguan metabolik kronis yang ditandai dengan kadar glukosa darah yang melebihi ambang normal. Tingginya angka penderita diabetes mendorong pengembangan pengobatan alternatif berbasis bahan alam. Salah satu tanaman potensial adalah buah dewandaru (Eugenia uniflora) yang mengandung senyawa bioaktif seperti flavonoid dan asam fenolat yang diketahui memiliki efek hipoglikemik. Penelitian ini bertujuan untuk menguji efektivitas ekstrak etanol buah dewandaru dalam menurunkan kadar glukosa darah pada mencit (Mus musculus) yang diinduksi aloksan. Penelitian ini menggunakan desain eksperimental Pretest-Posttest Only Control Group Design. Ekstrak buah dewandaru diperoleh melalui metode maserasi dan diberikan pada 24 ekor mencit yang dibagi menjadi 4 kelompok (masing-masing 6 ekor). Kelompok 1 (kontrol negatif) hanya menerima aloksan, kelompok 2 (kontrol positif) menerima glibenklamid 5 mg, kelompok 3 menerima ekstrak 100 mg/kgBB, dan kelompok 4 menerima ekstrak 200 mg/kgBB. Kadar glukosa darah diukur pada hari ke-3 (pretest) dan hari ke-14 (posttest) menggunakan glukometer. Data dianalisis dengan uji normalitas Shapiro-Wilk dan uji Paired T-Test menggunakan SPSS 26. Hasil penelitian menunjukkan adanya penurunan kadar glukosa darah yang signifikan (p < 0,05) pada kelompok mencit yang diberi ekstrak 100 mg/kgBB dan 200 mg/kgBB dibandingkan kelompok kontrol. Temuan ini menunjukkan bahwa ekstrak buah dewandaru berpotensi sebagai agen antidiabetes alami, sehingga penelitian lanjutan untuk isolasi senyawa aktif dan uji keamanan jangka panjang sangat dianjurkan.
Sweet Potato Leaf Extract Gummy Candy as an Antioxidant-Rich Functional Food for Stunting Prevention in Children Udayani, Ni Nyoman Wahyu; Gelgel Wirasuta, I Made Agus; Nindya Kartika, Dewa Ayu Kintan; Anggreni, Ayu Putu Puspa
Journal Syifa Sciences and Clinical Research Vol 7, No 3 (2025): Volume 7 Number 3 2025
Publisher : State University of Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/jsscr.v7i3.33862

Abstract

Stunting remains a major public health concern linked to oxidative stress and micronutrient deficiencies. This study aimed to develop and evaluate gummy candy formulations containing sweet potato leaf extract (Ipomoea batatas L.) as a functional food candidate for stunting prevention in children. The extract was obtained by maceration, and the formulation was evaluated through phytochemical screening, sensory evaluation, weight uniformity, antioxidant activity using the DPPH method, and mineral content analysis with atomic absorption spectrophotometry (AAS). Phytochemical analysis confirmed the presence of flavonoids, tannins, and saponins. Sensory evaluation by 30 panelists indicated observable differences between formulations: F0 (control) was preferred in terms of color and taste, while F1 (with extract) presented a darker green-purple appearance and a slightly bitter aftertaste, though texture remained similar in both. Weight uniformity results met pharmacopeial standards, with coefficient of variation values below 5%. Antioxidant activity testing revealed strong radical scavenging potential of the extract (IC₅₀: 29.263 ppm), moderate activity in the F1 gummy (IC₅₀: 108.245 ppm), and very weak activity in F0 (IC₅₀ 200 ppm), compared to ascorbic acid as a positive control (IC₅₀: 6.426 ppm). Mineral analysis of F1 showed iron content of 168.94 ppm and zinc content of 28.05 ppm, contributing significantly to daily nutritional requirements. In conclusion, the incorporation of sweet potato leaf extract into gummy candy provides bioactive compounds with antioxidant activity and essential minerals, while maintaining acceptable sensory quality. These findings support its potential development as a functional food to contribute to stunting prevention strategies in children.
Pemanfaatan Toga Usadha Bali bagi Pemuda Desa Adat Bitera Gianyar Menuju Era Normal Baru Ketut Agus Adrianta; I Gusti Agung Ayu Kusuma Wardhani; Ni Nyoman Wahyu Udayani; Agung Ari Chandra Wibawa
Jurnal Pengabdian UNDIKMA Vol. 4 No. 1 (2023): February
Publisher : LPPM Universitas Pendidikan Mandalika (UNDIKMA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/jpu.v4i1.5843

Abstract

This community service aims to increase knowledge and skills for the youth of the Bitera Gianyar traditional village regarding the use of TOGA (family medicinal plants) as well as provide training and assistance in making plant-based hand sanitizers in the yard of the house that can be used and used for preventive efforts against transmission the Covid-19 virus. The service method used was in the form of participatory training and small group discussions (SGD). The activity evaluation instrument was carried out using a questionnaire and statistically analyzed using a paired t-test. This community service activity increased participants' knowledge by 45.84%. The service participants, in general, understood the importance of vaccination for the human body and how to use materials around the environment as natural hand sanitizers. Increasing the ability and knowledge of the Bitera Gianyar traditional village's youth is expected to change people's behavior; henceforth, the participants will become agents of change who will influence their environment to adopt a healthy lifestyle.
Co-Authors Adnyani, Putu Mesi Dian Adrianta, I Ketut Agus Agung Ari Chandra Wibawa Agung Ari Chandra Wibawa Agustin, Ni Kadek Jessica Agustini, Ni Putu Dewi Anggreni, Ayu Putu Puspa Anita Dewi, Ni Luh Kade Arman Apriani, Rista Ciptawati, Ni Wayan Cokorda Javandira Debby Juliadi Dewi, Ni Kadek Dhea Cipta Dewi, Putu Intan Candra Dwipayanti, Ni Kadek Sari Erna Cahyaningsih Febryna Dharma Yanti, Ni Luh Putu Fitria Megawati Fitria Megawati Fredinan Yulianda Fridayana, Ni Luh Gede Erica Herleeyana Meriyani Herleeyana Meriyani I Dewa Ayu Anom Yustari Nida I Gede Made Suradnyana I Gede Yodhi Trismawan I Gusti Agung Ayu Kusuma Wardhani I Gusti Ayu Arya Ditha Suari I Ketut Sumantra I Made Agus Sunadi Putra I Made Agus Sunadi Putra I Made Agus Sunadi Putra I Made Agus Sunadi Putra I Nyoman Gede Tri Sutrisna I Putu Pasek Jaya Separsa I Putu Tangkas Suwantara I Putu Werda Bisama Ida Ayu Gede Syntya Mahayani IGAA Kusuma Wardani Kadek Ayu Puspa Pratiwi Ketut Agus Adrianta Ketut Agus Adrianta Ketut Agus Adrianta Ketut Agus Adrianta Ketut Agus Adrianta Ketut Agus Adrianta Ketut Agus Adrianta Laksmi, Luh Putri Dianti Lestari, Ketut Sita Citra Lilis Adnyani Ni Putu Luh Putu Febryana L Lusi Puspitasari, Ni Wayan Made Dwik Lestari Maharani, Ade Dwi Karisma Mahardika, I Made Agus Mahendra, I Putu Aldy Manik Suka Dewi Yani Ni Komang Maria Daniela Tupa Hokor Maria Malida Vernandes Sasadara Mayun Anak Agung Istri Mega Ratnasari, Ni Luh Ayu Megawati, Fitri Megawati, Fitria Meika, Ni Putu Trisna Mendra, Ni Nyoman Yudianti Meriyani, Herleeyana Nggaba, Ellenoire Rambu Kabeba Hammu Ni Komang Enny Wahyuni Ni Luh Ayu Mega Ratnasari Ni Luh Kade Arman Anita Dewi Ni Luh Kade Arman Anita Dewi Ni Luh Putu Dewi Purnama Sari Ni Luh Putu Sulasmini Ni Luh Putu Tris Yoga Cahyani Ni Made Dharma Shantini Suena Ni Made Dwi Mara Widyani Nayaka Ni Made Sukma Sanjiwani Ni Nyoman Ayu Puspita Sari Ni Putu Dewi Agustini Ni Putu Leona Yorita Dewi Ni Putu Lilis Adnyani Ni Putu Lilis Adnyani Ni Putu Putri Cahya Anggreni Ni Putu Refina Dharma Yanti Ni Putu Sintia Rahayu Ni Wayan Ciptawati Ni Wayan Riastini Nindya Kartika, Dewa Ayu Kintan Nugraha, Nyoman Dhamapada Nyoman Budiartha Siada Pendet, Ni Made Diah Pusparini Puguh Santoso Puguh Santoso puguh santoso, puguh Putra Hilmi Prayitno Putra, I Made Agus Sunadi Putri, Ni Komang Ary Agustina Berliana Putu Era Sandhi K.Yuda Putu Era Sandhi Kusuma Yuda Putu Mesi Dian Adnyani Riastini, Ni Wayan Sasadara, Maria Malida Vernandes Siluh Putu Astini Sugijanto, Milyadi Sunadi Putra I Made Agus Wardani, I Gusti Agung Ayu Kusuma Widiasriani, Ida Ayu Putu Wiguna, I Putu Bagus Artha Wiguna, Putu Dony Saputra Woro Enjang Santi Prihatin Wulandari, Ni Kadek Ganis Yowani, Anak Agung Sagung Iswarani Yustari Nida, I Dewa Ayu Anom Yuwono, Moch.