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Aktivitas Sitotoksik Ekstrak n-Heksana, Diklorometana, dan Metanol Daun Beluntas (Pluchea indica Less.) terhadap Sel Kanker Leher Rahim (HeLa) Puspitasari, Endah; Agustina, Bayu; ., Nuri; Ulfa, Evi Umayah
Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 2 No. 1 (2015)
Publisher : Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jfst.v2i1.810

Abstract

Eksplorasi agen kemopreventif kanker masih terus dikembangkan, terutama yang berasal dari bahan alam. Hal ini didasari oleh keinginan untuk menekan angka kematian akibat kanker dan mengurangi efek samping yang ditimbulkan oleh terapi kanker yang saat ini digunakan. Penelitian ini ditujukan untuk mengetahui aktivitas sitotoksik daun beluntas (Pluchea indica Less.) terhadap sel kanker leher rahim yang merupakan kanker ginekologi peringkat pertama yang menyerang wanita. Aktivitas sitotoksik dilakukan terhadap ekstrak n-heksana, diklorometana, dan metanol daun beluntas terhadap sel HeLa dengan metode perhitungan langsung menggunakan bantuan perwarna trypan blue. Nilai IC50 dari ketiga ekstrak daun beluntas berturut turut adalah 18,06 μg/ml, 74,56 μg/ml, dan 31,21 μg/ml. Ketiganya memberikan perbedaan yang signifikan. Ketiga ekstrak yang diuji memiliki aktivitas sitotoksik dan dapat dikembangkan sebagai kandidat agen kemopreventif kanker. Namun masih perlu diteliti lebih lanjut bagaimana mekanisme aksi yang menjadi perantara aktivitas sitotoksik tersebut.
PENGARUH KOMISARIS INDEPENDEN DAN KEPEMILIKAN INSTITUSIONAL TERHADAP KUALITAS LABA (Suatu Studi Pada Perusahaan Pertambangan yang Terdaftar di Bursa Efek Indonesia Tahun 2019) puspitasari, endah; Usmar, Dani; Faris Rudiana, Irfan
Jurnal Industrial Galuh Vol. 6 No. 1 (2024): Jurnal Industrial Galuh
Publisher : Teknik Industri Fakultas Teknik UNIGAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25157/jig.v6i1.3797

Abstract

Pemahaman mengenai bagaimana menyusun suatu mekanisme tata kelola yang baik dalam rangka meningkatkan kualitas laba yang dilaporkan sangatlah penting. Adapun tujuan penelitian ini adalah untuk mengetahui: 1) Pengaruh komisaris independen terhadap kualitas laba pada perusahaan pertambangan di Bursa Efek Indonesia tahun 2019, 2) Pengaruh kepemilikan institusional terhadap kualitas laba pada perusahaan pertambangan di Bursa Efek Indonesia tahun 2019, 3) Besarnya pengaruh komisaris independen dan kepemilikan institusional terhadap kualitas laba pada perusahaan pertambangan di Bursa Efek Indonesia tahun 2019. Populasi dalam penelitian ini adalah perusahaan Subsektor Pertambangan yang terdaftar di Bursa Efek Indonesia sebanyak 42 perusahaan. Berdasarkan proses penentuan sampel terdapat 13 perusahaan Subsektor Pertambangan yang terdaftar di Bursa Efek Indonesia yang memenuhi kriteria sampel. Metode yang digunakan dalam penelitian ini adalah analisis deskripstif dengan pendekatan kuantitatif. Sedangkan untuk menganalisis data yang diperoleh digunakan Analisis Koefisien Korelasi, Analisis Regresi Ganda, Analisis Koefisien Determinasi, dan Uji Hipotesis menggunakan Uji Signifikansi (Uji t), dan Uji Hipotesis menggunakan Uji Signifikansi (Uji F). Hasil dari penelitian dan pengolahan data menunjukan bahwa 1) Komisaris independen berpengaruh negatif terhadap kualitas laba dengan memperoleh nilai signifikan (-0,706 < 2,201), 2) Kepemilikan institusional b erpengaruh negatif terhadap kualitas laba dengan memperoleh nilai signifikan (-0,076 < 2,201), 3) Komisaris independen dan kepemilikan institusional berpengaruh negatif terhadap kualitas laba dengan memperoleh nilai signifikan (0,23 < 3,02). Bagi perusahaan diharapkan kurangi melakukan pengangkatan dewan komisaris yang didasarkan pada hubungan dekat dan hubungan keluarga (relasi) serta tidak melakukan manipulasi terhadap laporan keuangan.
Formulasi Krim Ekstrak Daun Jambu Biji (Psidium guajava L.) dan Avobenzone sebagai Sediaan Tabir Surya Nurjannah, Siti; Wisudyaningsih, Budipratiwi; Puspitasari, Endah
Journal of Agropharmacy Vol. 1 No. 3 (2024)
Publisher : Faculty of Pharmacy, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/joa.v1i3.1394

Abstract

Skin exposed to ultraviolet (UV) rays for a long period of time can cause adverse effects on the skin such as erythema, premature aging and skin cancer so that the use of sunscreen is needed to protect the skin from UV exposure. Sunscreen is used to protect the skin from damage caused by UV exposure by absorbing and scattering UV rays that hit the skin. The study was conducted by making a formulation of guava leaf extract cream and avobenzone in a sunscreen cream preparation, evaluating physical quality, evaluating the in vitro effectiveness of sunscreen preparations including SPF,% TE, and% TP values ​​and data analysis. Sunscreen cream preparations with additional concentrations of guava leaf extract can reduce pH and spreadability, TE and TP but can increase viscosity and SPF values. Formula F3 (7%) has physical quality that meets the criteria of the expected cream preparation and can increase the effectiveness in vitro in sunscreen cream preparations and get the best results with an SPF value of 18.49 ± 1.02 which is included in the ultra protection category and a decrease in the %TE value of 0.486 ± 0.004 and a decrease in %TP of 0.158 ± 0.005 which is included in the total block category. It is recommended in further research to conduct compatibility tests and stability tests of guava leaf extract and avobenzone sunscreen cream preparations.
Validasi Metode Penentuan Kadar Asam Klorogenat dan Kafein secara Simultan pada Serbuk Daun Kopi Robusta (Coffea Canephora) dengan Metode KLT Densitometri Putri Aditama, Tiara Sagita; Kristiningrum, Nia; Wulandari, Lestyo; Holidah, Diana; Alishlah, Tanfidz; Puspitasari, Endah
Journal of Agropharmacy Vol. 2 No. 1 (2025)
Publisher : Faculty of Pharmacy, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Coffee contains bioactive compounds such as chlorogenic acid and caffeine. Chlorogenic acid is recognized as a natural antioxidant, while caffeine is widely used to reduce fatigue and drowsiness. This study aims to simultaneously determine the content of chlorogenic acid and caffeine in robusta coffee leaf powder (Coffea canephora) using Thin Layer Chromatography (TLC) densitometry. The study identified an optimal eluent composition of ethyl acetate:formic acid:distilled water (18:1:1), with maximum wavelengths of 330 nm for chlorogenic acid and 274 nm for caffeine. High linearity was demonstrated with correlation coefficients of 0.993 for chlorogenic acid and 0.994 for caffeine. Sensitivity was validated with LOD values of 100.664 ng for chlorogenic acid and 297.232 ng for caffeine, and LOQ values of 301.994 ng for chlorogenic acid and 891.697 ng for caffeine. Selectivity was confirmed as the method effectively separated chlorogenic acid, caffeine, and other compounds with a resolution (Rs) ≥ 1.5, while specificity was evidenced through purity and identity values exceeding 0.99. Precision was proven through repeatability (%RSD for chlorogenic acid: 2.94%; caffeine: 2.27%) and intermediate precision (%RSD for chlorogenic acid: 2.26%; caffeine: 2.18%). Accuracy was achieved with mean recovery rates of 102.22% for chlorogenic acid and 99.73% for caffeine. The determination of chlorogenic acid and caffeine concentrations in robusta coffee leaf powder revealed variations between samples: PTPN XII Renteng yielded 2.278% ± 0.209% for chlorogenic acid and 1.171% ± 0.821% for caffeine, while Puslitkoka Indonesia samples showed 1.238% ± 0.206% for chlorogenic acid and 0.917% ± 0.823% for caffeine. Based on validation parameter assessments, the method is deemed valid as it meets all acceptance criteria for each parameter. This research provides an efficient and accurate analytical approach for simultaneous determination of bioactive compounds in robusta coffee leaves.
Biosynthesis of AgNPs Mediated by Equisetum debile Roxb. Herb Water Extract and Its Potency as an Antioxidant Retnaningtyas, Yuni; Karmilasari, Alviani Dwi; Puspitasari, Endah
Pharmaceutical Sciences and Research Vol. 11, No. 2
Publisher : UI Scholars Hub

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Abstract

Silver nanoparticles (AgNPs) are metal nanoparticles currently under development. AgNPs are recognized for their remarkable stability, low reactivity, and advantageous biological properties, including antioxidant activity. Phenolic compounds derived from the Equisetum debile Roxb. (Ed-R) herb have the potential to be explored as bio-reduction and capping agents in the synthesis of silver nanoparticles. The primary objective of this study is to synthesize AgNPs using a water extract from Ed-R herb and to assess their antioxidant activity. The optimal composition for the biosynthesis of AgNPs comprises 10% Ed-R water extract, 8.0 mM AgNO3, and deionized water in a ratio of 0.5:20:12 (v/v/v), with the mixture incubated for 60 minutes at 50°C. AgNPs were characterized by conducted using UV-Vis Spectrophotometry, FT-IR, Scanning SEM, and PSA. The total phenolic content (TPC) of the Ed-R herb water extract at the optimal concentration (10%) was determined to be 61.503 ± 0.089 mg GAE 100 mL-1. The characterization results indicated that the AgNPs exhibited a spherical morphology, with a particle size of 67 nm, a polydispersity index (PDI) of 0.424, a maximum wavelength of 423 nm, and an absorbance value of 1.397. The antioxidant activity of AgNPs was evaluated using the DPPH method, resulting in an IC50 value of 76.604 ± 0.846 μg mL-1, which signifies strong antioxidant activity. These findings suggest that the water extract from the Ed-R herb can effectively act as both bioreduction and capping agent in the synthesis of AgNPs, which demonstrates significant antioxidant properties.
ANALISIS EFISIENSI TEKNIS PENGGUNAAN FAKTOR-FAKTOR PRODUKSI USAHATANI PADI PRA IMPLEMENTASI PERLINDUNGAN LAHAN PANGAN PERTANIAN BERKELANJUTAN (Kasus Petani di Desa Jati Kecamatan Tarogong Kaler Kabupaten Garut Jawa Barat) Nahraeni, Wini; Masithoh, Siti; Puspitasari, Endah
JURNAL AGRIBISAINS Vol. 2 No. 1 (2016): Jurnal AgribiSains
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.582 KB) | DOI: 10.30997/jagi.v2i1.763

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan faktor-faktor produksi terhadap produksi padi, tingkat efisiensi teknis dan efisiensi ekonomis usahatani padi. Pengumpulan data dilaksanakan pada November – Desember 2015 di Desa Jati Kecamatan Tarogong Kaler Kabupaten Garut Provinsi Jawa Barat. Data dianalisis menggunakan fungsi produksi Cobb-Douglas yang diolah dengan menggunakan aplikasi SPSS 16.0. Hasil penelitian menunjukkan bahwa variabel luas lahan, jumlah benih, tenaga kerja, pengalaman, status petani serta penyuluhan masing-masing berpengaruh positif terhadap produksi padi dan signifikan pada taraf α dibawah 10 persen, kecuali pupuk N dan pupuk P. Variabel yang bernilai negatif dan tidak signifikan terhadap produksi padi adalah pupuk N. Pada aspek efisiensi ekonomis, usahatani padi yang dilakukan di Desa Jati belum efisien, hal ini dapat dilihat dari rasio NPM/BKM yang lebih besar atau lebih kecil dari satu. Variabel luas lahan dan benih mempunyai rasio NPM/BKM lebih dari satu sehingga variabel-variabel tersebut masih dapat ditambah agar dapat efisien, sedangkan tenaga kerja mempunyai rasio NPM/BKM kurang dari satu, sehingga input variabel tersebut dapat dikatakan sudah berlebih, agar input variabel ini efisien maka variabel ini dapat dikurangi. Lahan mempunyai elastisitas paling tinggi dalam meningkatkan nilai produksi padi, maka program pemerintah untuk melindungi lahan pertanian dari pengalihan fungsi melalui program PLP2B sangat relevan. Kata kunci : Efisiensi teknik, Nilai Produk Marjinal, Cobb-Douglas
Pelatihan Pembuatan Jamu Tradisional di Kelurahan Karangrejo, Sumbersari, Jember Dianasari, Dewi; Puspitasari, Endah; Triatmoko, Bawon
ABDIMASKU : JURNAL PENGABDIAN MASYARAKAT Vol 6, No 2 (2023): Mei 2023
Publisher : LPPM UNIVERSITAS DIAN NUSWANTORO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33633/ja.v6i2.1058

Abstract

Beberapa sentra usaha terdapat di Kabupaten Jember, salah satunya berada di Kelurahan Karangrejo, Kecamatan Sumbersari, Kabupaten Jember. Salah satunya adalah usaha produksi jamu tradisional yang dijalankan oleh kelompok mitra, yakni ibu-ibu warga RT 02/ RW07 Lingkungan Kalikotok, Kelurahan Karangrejo, Kecamatan Sumbersari, Kabupaten Jember. Permasalahan yang dihadapi adalah kurangnya pengetahuan mitra terkait proses produksi dan pengemasan produk   yang higienis agar menghasilkan jamu yang aman, bermutu, dan berkhasiat, serta teknik pemasaran produk yang efektif dan efisien. Untuk mengatasi permasalahan tersebut, maka dilakukan kegiatan program pengabdian kepada masyarakat. Kegiatan pengabdian yang telah dilaksanakan antara lain penyuluhan tentang tanaman obat dan obat tradisional, pelatihan dan praktik CPOTB, pelatihan dan praktik produksi jamu dan pengemasannya, serta pemasaran produk jamu. Kegiatan ini dapat mengatasi permasalahan mitra , menghasilkan produk jamu yang aman,bermutu dan berkualitas,  sekaligus dapat meningkatkan perekonomian mitra.
Pelatihan Pembuatan Serbuk Jamu Instan di Kelurahan Karangrejo, Kecamatan Sumbersari, Kabupaten Jember Dianasari, Dewi; Puspitasari, Endah; Nuri, Nuri; Triatmoko, Bawon; Muslichah, Siti
ABDIMASKU : JURNAL PENGABDIAN MASYARAKAT Vol 7, No 1 (2024): JANUARI 2024
Publisher : LPPM UNIVERSITAS DIAN NUSWANTORO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62411/ja.v7i1.1835

Abstract

Salah satu jenis olahan tanaman obat adalah dalam bentuk jamu. Salah satu sentra usaha produksi jamu tradisional dijalankan oleh ibu-ibu warga RT 02/ RW07 Lingkungan Kalikotok, Kelurahan Karangrejo, Kecamatan Sumbersari,Kabupaten Jember. Permasalahan yang dihadapi mitra yaitu produk jamu yang akan dibuang jika tidak laku pada hari tersebut, karena masa simpan jamu yang sangat singkat dan akan berubah rasa. Untuk mengatasi tersebut, maka perlu dilaksanakan kegiatan pengabdian berupa pelatihan diversifikasi produk olahan jamu berupa pembuatan serbuk jamu instan, cara pengemasan dan pemasarannya. Pelatihan dilaksanakan dengan metode demonstrasi langsung pembuatan dan pengemasan serbuk jamu jahe wangi instan, serta pemaparan materi tentang cara pemasarana yang efektif dan efisien.Kegiatan pelatihan dapat meningkatkan pengetahuan, mengatasi permasalahan mitra, dan juga dapat meningkatkan omset penjualan mitra, sekaligus meningkatkan kesejahteraan mitra.Kata kunci: serbuk jamu instan, jamu
Antiproliferative activity of Ethanolic Extract of Kembang Bulan (Tithonia diversifolia) Leaf on HeLa Cervical Cancer Cell Line Puspitasari, Endah; Nuri, Nuri; Ningsih, Indah Yulia; Triatmoko, Bawon; Dianasari, Dewi
Indonesian Journal of Cancer Chemoprevention Vol 13, No 1 (2022)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev13iss1pp55-60

Abstract

Tithonia diversifolia has been showed to be cytotoxic and antiproliferative on colon cancer, glioblastoma, hepatoma, kidney cancer, breast cancer, lung cancer, melanoma, leukemia, ovary cancer, prostate cancer, and stomach cancer cell lines, but not on cervical cancer cells yet. Our research aimed to determine the cytotoxicity and antiproliferative activity of T.diversifolia leaf ethanolic extract on HeLa cervical cancer cell line. The cytotoxicity and the antiproferative activity assay were done using MTT method for 24 h for cytotoxic assay; and series of 24, 48, and 72 h for antiproliferative assay. The cytotoxic activity was analyzed using IC50, while the antiproliferative assay was analyzed based on the proliferation kinetics. All assays were done in triplicate. T.diversifolia leaf ethanolic extract exhibited strong cytotoxic activity on HeLa cervical cancer cell lines with the IC50 of 97.839±10.120 μg/mL. The cytotoxic activity was dose dependent. Based on the proliferation assay, the antiproliferative activity was stronger as the incubation time and the dose increases. T.diversifolia leaf ethanolic extract showed strong cytotoxic and antiproliferative activity on HeLa cervical cancer cell lines.Keywords: T.diversifolia leaf ethanolic extract, cytotoxicity assay, antiproliferative assay, HeLa cervical cancer cells.
Cytotoxic Potential of Flavonoid from Nicotiana tabacum Leaves on MCF-7 Human Breast Cancer Cells Kusumawardani, Banun; Febi, Qonita Nafilah; Rosidah, Malihatul; Azis, Deri Abdul; Puspitasari, Endah; Nugraha, Ari Satia
Indonesian Journal of Cancer Chemoprevention Vol 11, No 2 (2020)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev11iss2pp97-10

Abstract

Flavonoid has potential bioactivity as anticancer agents. The flavonoid of cultivated tobacco (Nicotiana tabacum), locally known as “Kasturi”, leaves was screened for its cytotoxicity against MCF-7 human breast cancer cells and non-transformed Vero cells (African normal cell kidney line) in different concentrations. This study aimed to examine the cytotoxic potential of the flavonoid of Kasturi tobacco leaves against MCF-7 human breast cancer cells. Flavonoid obtained from methanolic extracts of Kasturi tobacco leaves, which have been purified from nicotine. The flavonoid of Kasturi tobacco leaves with concentrations of 20 to 640 μg/mL were exposed to MCF-7 and Vero cells for 24 h. Cell viability was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Flavonoid of Kasturi tobacco leaves with concentrations of 160 μg/mL decreased the MCF-7 cell viability more than 50%, with an inhibitory concentration 50 (IC50) value of 148.41 μg/mL. Meanwhile, it inhibited 50% of Vero cell viability at 255.35 μg/mL. The flavonoid of Kasturi tobacco leaves has cytotoxic activity on MCF-7 cells, and might be a potential alternative agent for human breast cancer therapy.Keywords: flavonoid, tobacco leaves, human breast cancer cells, anticancer activity
Co-Authors . Anindyajati Aditya Fitriasari Aditya Fitriasari Agustina, Bayu Agustina, Bayu Anandini Aulia Safiera Anindyajati Anindyajati Ari Satia Nugraha Azis, Deri Abdul Banun Kusumawardani Baroroh, Rizqy Kiromin Bawon Triatmoko Bawon Triatmoko Bawon Triatmoko Binar Asrining Dhiani Dani Usmar Dani Usmar, Dani Dea Ajeng Pravita Suendi Dewi Dianasari Dewi Dianasari Dewi Dianasari Diana Holidah Dianasari, Dewi Dwi Merry Christmarini Robin Dwi Novita Wijayanti Dyaningtyas D. P. Putri Dyaningtyas Dewi P. Putri Dyaningtyas Dewi Putri Pamungkas Edy Meiyanto Edy Meiyanto Endah Dwi Setyowati Endah Dwi Setyowati ESTIKA YUNINDARWATI Evi Umayah Ulfa Fadhila, Nadila Ikhda Ramadhani Faris Rudiana, Irfan Fatimatuz Zuhro Fauzia Ken Nasititi Febi, Qonita Nafilah Fifteen Aprila Fajrin Hasanah, Fitrianti Milhatul Hidayatul Ulyah Indah Yulia Ningsih Irma Josefina Savitri Karmilasari, Alviani Dwi Labes, Antje Lestyo Wulandari Lukmanto, Lukmanto Maharany, Dinda Mochammad Amrun Hidayat Muhammad Zaki Rahman Nahraeni, Wini Nasititi, Fauzia Ken Nia Kristiningrum Novan Eko Setiyanggono Novia Hilma Nur Fauziah Matra Nur Ismiyati Nur Ismiyati Nuri . Nuri . Nuri Nuri Nuri Nuri Nuri Nuri Nuri Nuri Oktarianti, Rike Oktavia, Melinda Dwi Putri Putri Aditama, Tiara Sagita Ramadhan, Andika Dewi Rifki Febriansah Rifki Febriansah Rosidah, Malihatul Rudiana, Irfan Faris Sarmoko Sarmoko Senjarini, Kartika Siti Masithoh Siti Muslichah Siti Nurjannah Siti Sarah, Siti Sugeng Riyanto Sugeng Riyanto Tanfidz Alishlah, Tanfidz Tazkiyatul Fitriyah Tri Agus Siswoyo Tsabit Barki Viddy Agustian Rosyidi Wathon, Syubbanul Yuni Retnaningtyas Zahra, Nurul Fatimatuz