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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Hortikultura Indonesia (JHI) Vegetalika Zuriat Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat JURNAL AGROTEKNOLOGI Gontor AGROTECH Science Journal Jurnal Sains Teh dan Kina Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Journal of Tropical Crop Science JURNAL AGRONIDA JURNAL BUDIDAYA PERTANIAN Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Agrotech Journal
Yudiwanti Wahyu E. Kusumo
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural Universtity), Jl Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Industrial & Manufacturing Engineering Mechanical Engineering

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Histological Analysis of Somatic Embryogenesis from Immature Zygotic Embryo of Wild Banana Musa acuminata ssp. malaccensis Handayani, Tri; Martanti, Diyah; Prawestri, Apriliana Dyah; Tihurua, Eka Fatmawati; Maharijaya, Awang; Wahyu, Yudiwanti; Sobir; Witjaksono
Journal of Tropical Crop Science Vol. 11 No. 03 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.03.307-319

Abstract

Somatic embryogenesis, a crucial plant regeneration method, has become indispensable for crop improvement, particularly for species reliant on somatic cell manipulation techniques. Optimization of this process necessitates an understanding of the developmental stages involved. This study investigates the histological aspects of somatic embryogenesis in Musa acuminata ssp. malaccensis derived from immature zygotic embryos. Through detailed histological analysis, we aimed to elucidate the morphological changes and cellular organization occurring during the various stages of somatic embryogenesis, from induction, culture proliferation, and somatic embryo development to plantlet conversion. The initial stages of embryogenesis, characterized by nodules, were primarily composed of meristematic cells with high cell division activity. These cells contained tetrad-like structures that could develop into distinct two- and four-celled proembryoids or proembryogenic aggregates. Our histo-anatomical analysis revealed that embryogenic cultures proliferated through multiple pathways simultaneously: somatic embryo budding, proembryo formation, and pro-embryonic mass formation from both internal and peripheral cells. At the stage of somatic embryo development, embryos with a well-defined protoderm layer, containing cells with prominent nuclei and dense cytoplasm, potentially regenerate into plantlets. Furthermore, histological examination revealed the presence of procambium within mature somatic embryos, which subsequently developed into the vascular system of the complete plantlet
Evaluasi Segregan Transgresif pada Dua Populasi Cabai Rawit (Capsicum frutescens L.) Hakim, Abdul; Syukur, Muhamad; Wahyu, Yudiwanti
Vegetalika Vol 13, No 4 (2024): In Publish
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.93776

Abstract

Kemajuan seleksi menunjukkan sejauh mana nilai peningkatan yang diperoleh dari karakter yang diseleksi. Kemajuan seleksi dipengaruhi oleh intensitas seleksi, ragam dan heritabilitas. Percobaan ini bertujuan untuk memperoleh kandidat genotipe segregan transgresif pada populasi terseleksi cabai rawit. Hasil dari penelitian ini pada populasi F3-285290 dan F3-C321290 nilai kemajuan seleksinya positif untuk semua karakter kecuali untuk umur berbunga dan umur panen. Nilai heritabilitas populasi F3-285290 termasuk dalam kategori sedang untuk karakter bobot per buah, panjang tangkai buah dan jumlah buah per tanaman. Sedangkan nilai heritabilitas pada populasi F3-321290 yang termasuk kategori tinggi terdapat pada karakter umur panen, bobot per buah, panjang buah, diameter buah dan panjang tangkai buah. Genotipe segregan transgresif pada populasi F3-285290 terdapat pada karakter panjang buah, tebal daging buah dan panjang tangkai buah. Genotipe yang terverifikasi segregan transgresif hanya pada karakter panjang buah yang terdapat pada tiga genotipe yaitu F3-285290-205, F3-285290-248 dan F3-285290-257. Pada populasi F3-321290 genotipe segregan transgresif terdapat pada karakter tebal daging buah, panjang tangkai buah dan bobot buah per tanaman. Genotipe yang terverifikasi segregan transgresif hanya pada karakter bobot buah per tanaman yang terdapat pada dua genotipe yaitu F3-321290-33 dan F3-321290-293. Adanya informasi segregan transgresif pada kegiatan pemuliaan cabai rawit ini dapat dijadikan sebagai akselerasi dalam memperoleh kandidat calon varietas. 
Discovery of Simple Sequence Repeat Markers in the Endemic Durian Kura-Kura (Durio testudinarius Becc.) Magandhi, Mahat; Sobir; Matra, Deden Derajat; Wahyu, Yudiwanti; Sudarmono
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.132-139

Abstract

Durio testudinarius, known as Durian Kura-Kura, is a native Bornean durian species in the Malvaceae family with fruit at the base of the main stem. Recent advances in genomic-based next-generation sequencing are being used to conserve germplasm and enhance plant breeding. This method rapidly and cost-effectively sequences plant genomes, allowing for simple sequence repeat markers to be constructed in silico. This study aimed to design and evaluate novel, simple sequence repeat markers utilizing next-generation sequencing microsatellite data of D. testudinarius. A total of 20 sequences containing di-, tri-, tetra-, and penta-nucleotide motif repeats were selected and designed to generate primers using Primer3 online software. The PCR results of the twenty primers tested on D. testudinarius and 19 genomic DNA samples of species from the Malvales order showed successful amplification. These newly developed simple sequence repeat markers are crucial for genetic population analyses, determining population structure, and enhancing plant breeding programs and conservation strategies for D. testudinarius and its relative's germplasm resources.
An Efficient Somatic Embryogenesis and Plant Regeneration from Immature Embryo of Wild Banana Musa acuminata ssp. malaccensis Handayani, Tri; Martanti, Diyah; Prawestri, Apriliana Dyah; Maharijaya, Awang; Wahyu, Yudiwanti; Sobir; Witjaksono
Journal of Tropical Crop Science Vol. 12 No. 01 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.01.172-184

Abstract

Wild banana Musa acuminata ssp. malaccensis, an ancestor of cultivated bananas, possesses valuable genetic diversity, including resistance genes to fusarium wilt, and demonstrates high environmental adaptability. These traits are important for pre-breeding programs, whether by conventional breeding, which is a lengthy process, or by taking advantage of somatic cell manipulation techniques such as somatic hybridization, which requires an efficient plant regeneration system like somatic embryogenesis. We have established an efficient and comprehensive protocol for somatic embryogenesis of this wild Musa using immature zygotic embryo explants covering culture induction and proliferation, somatic embryo development, and subsequent plant conversion. Embryogenic culture was induced on a complex-modified MS medium supplemented with 1 mg.L-1 2,4-D auxin or 5 mg.L-1 picloram. The embryogenic cultures proliferated in the form of granular or nodular structures, which was best obtained by reducing the picloram concentration to 1 mg.L-1 and combining it with the same concentration of 2,4-D at a half-strength macro-nutrient of basal medium. Embryo development from embryogenic cultures and regeneration into shoots. Proembryos as granular structures spontaneously matured into early-stage somatic embryos upon withdrawal of the strong auxin inducer. Increasing the sucrose and gelling agent concentrations in the growth medium improved somatic embryo formation from embryogenic cultures. The frequency of shoot formation from developed somatic embryos was increased by incorporating 0.5 mg.L-1 BA and 0.5 mg.L-1 GA3 into the regeneration medium.
Seed Diversity Five Species of Chili (Capsicum spp.) Based on Morphological Characters and Seed Viability: Keanekaragaman Benih Lima Spesies Cabai (Capsicum spp.) Melalui Karakter Morfologi dan Viabilitas Benih Undang; Muhamad Syukur; Yudiwanti Wahyu; Abdul Qadir; Siti Marwiyah; Punjung Medaraji Suwarno
Jurnal Hortikultura Indonesia (JHI) Vol 16 No 1 (2025): Jurnal Hortikultura Indonesia (JHI)
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.16.1.47-57

Abstract

Benih cabai memiliki banyak keragaman dan merupakan salah satu kunci keberhasilan dalam budidaya. Identifikasi keragaman benih cabai, pengujian viabilitas benih diperlukan sebagai langkah dalam perakitan varietas dan penyediaan benih bermutu. Penelitian bertujuan memperoleh informasi bentuk biji, tonjolan paruh, bobot 1000 biji dan viabilitas benih. Penelitian dilakukan terhadap 63 genotipe cabai dari 5 spesies (Capsicum annuum, C. frutescens, C. chinense, C. baccatum, dan C. pubescens) bulan Februari-Juli 2023 di Laboratorium Ilmu dan Teknologi Benih IPB, menggunakan rancangan kelompok lengkap teracak 3 ulangan. Pengamatan meliputi sifat morfologi dan fisiologi benih cabai. Biji cabai secara umum berwarna putih kekuningan dengan tonjolan paruh, kecuali C. pubescens (warna biji hitam, tidak ada tonjolan paruh), bentuk biji seperti ginjal (C. annuum), lonjong (C. baccatum), tetes air mata (C. frutescens), berbentuk D (C. pubescens), melingkar dengan mulut ikan (C. chinense). Spesies cabai yang diamati memiliki berbagai ukuran bobot 1,000 biji (ringan (<3.90 g), sedang (3.90-5.68 g), dan berat (>5.68 g). Keragaman genetik perkecambahan, potensi tumbuh maksimum, indeks vigor, kecepatan tumbuh, keserempakan tumbuh berkategori luas, sedangkan bobot kering kecambah normal, laju pertumbuhan, bobot 1000 biji berkategori sempit. Ukuran biji tidak mempengaruhi persentase perkecambahan benih. Spesies C. pubescens memiliki karakteristik benih yang berbeda dengan spesies Capsicum lainnya, sedangkan spesies C. annuum memiliki kemiripan dengan C. frutescens, C. chinense, dan C. baccatum. Kata kunci: benih, cabai, keanekaragaman, morfologi, spesies
Extension of the Eeigenvalue-Based Selection Index Method for Fixation of Multiple Trait-Transgressive Segregates in Mungbeans (Vigna radiata L. Wilczek) Jambormias, Edizon; Sutjahjo, Surjono H; Mattjik, Ahmad A; Wahyu, Yudiwanti; Wirnas, Desta; Patty, Jacob R
JURNAL BUDIDAYA PERTANIAN Vol 20 No 1 (2024): Jurnal Budidaya Pertanian
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jbdp.2024.20.1.32

Abstract

The selection of the early generation is a viable strategy for the rapid development of new plant varieties. In mungbeans (Vigna radiata L. Wilczek), the selection process is carried out to obtain high seed yield, early maturity, and sufficiently large seed size. To enhance this process, the extension of the combined-eigenanalysis selection index method (CESIM) with transgressive segregation variables can be used to fix multiple-trait transgressive segregates. Therefore, this research aimed to (1) develop an eigenvalue-based selection index method in information from relatives analysis capable of fixing multiple-trait transgressive segregates in the early generation, (2) predict expected and realized multiple-trait selection responses, and (3) verify the presence of multiple-trait transgressive segregates in the early generation of mungbeans. The material used consisted of the F3 generation population and its selection outcomes in F4, originating from the crossbreeding of mungbean varieties Gelatik × Lasafu Lere Butsiw. The empirical breeding values (EBV) between and within families were obtained using the BLUP values of the F3 generation from the mixed linear model with a nested design and log-normal distribution. The EBV values between families and dummy variables of transgressive segregate families were used in the selection process with CESIM. Furthermore, analysis of variance in EBV values within families in the F4 generation was applied to verify the presence of multiple-trait transgressive segregates. The results showed that the selection from the best CESIM equation yielded index scores with a determination coefficient R2 = 97.76% and an expected selection response of 136.62. The verification process confirmed the presence of 9 families as multiple-trait transgressive segregate families in the early generation.
Keragaan Galur-galur Kacang Bambara (Vigna subterranea L.) Asal Lanras Sumedang Nisa, Febianti Khoirun; Wahyu, Yudiwanti; Ilyas, Satriyas
Buletin Agrohorti Vol. 13 No. 2 (2025): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v13i2.65578

Abstract

Kacang bambara merupakan salah satu jenis legum yang dibudidayakan di Indonesia dan telah dikembangkan untuk mendapatkan genotipe unggul melalui seleksi galur murni. Tujuan penelitian ini adalah mengevaluasi keragaan karakter kuantitatif dan kualitatif galur-galur kacang bambara asal lanras Sumedang serta untuk memperoleh korelasi antar karakter pada galur-galur tersebut. Penelitian ini dilaksanakan di Kebun Percobaan Cikarawang pada Januari–Juli 2022. Rancangan penelitian ini adalah RKLT dua ulangan dengan 14 galur asal lanras Sumedang, satu galur asal lanras Sukabumi dan satu populasi Sumedang yang belum diseleksi. Satu galur asal Sukabumi telah diseleksi galur murni dan digunakan sebagai pembanding. Hasil penelitian ini menunjukkan bahwa tinggi tanaman, umur berbunga, bobot 100 biji berbeda nyata antar galur yang diamati. Bobot 100 biji memiliki nilai duga heritabilitas arti luas kategori tinggi. Koefisien keragaman genetik seluruh karakter berada pada kategori rendah–sedang. Karakter-karakter pada komponen hasil saling berkorelasi positif. Umur berbunga berkorelasi negatif terhadap karakter tinggi tanaman, jumlah daun dan lebar kanopi pengukuran pertama, bobot polong total, bobot polong bernas, dan bobot 100 biji. Tipe kanopi, bentuk daun, bentuk polong, warna polong, tekstur polong serta warna dan corak biji menunjukkan keragaman antara galur uji dengan galur pembanding. Kata kunci : aksesi, heritabilitas, korelasi, polong
Evaluation of Agronomic Performance and Genetic Diversity in Indonesian Pigmented Rice Using SCoT (Start Codon Targeted) Markers Safitri, Vika Ayu; Fatimah, Fatimah; Wahyu, Yudiwanti; Ardie, Sintho Wahyuning; Mahrup, Mahrup
HAYATI Journal of Biosciences Vol. 33 No. 1 (2026): January 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.1.103-113

Abstract

Indonesia is rich in biodiversity, including rice (Oryza sativa L.), one of the most important and widely cultivated crops. However, the utilization of local Indonesian rice germplasm in breeding programs remains limited due to the lack of reliable characterization data. Therefore, it’s essential to establish a reliable method to ensure variety protection by breeders. In addition, understanding the relationship between morphological traits and genetic diversity is crucial for a targeted breeding study aimed at evaluating genetic diversity among 12 local rice genotypes from Indonesia through agronomic traits and molecular analysis using start codon targeted (SCoT) markers. Statistical analyses, including cluster and diversity indices, were applied to interpret the data. Characterization was conducted based on the descriptors from The International Rice Research Institute (IRRI) and Bioversity International, while molecular analysis employed 23 selected SCoT primers. The effectiveness of the markers was assessed through polymorphism percentage, PIC values, and cluster analysis. A polymorphism level of 69% was observed, with PIC values ranging from 0.3610 to 0.4646, suggesting that the SCoT markers used provided adequate informativeness for diversity analysis. The study revealed high genetic variability, with heritable traits and informative SCoT markers, demonstrating their potential for pre-breeding and diversity analysis.  
Co-Authors , Rosita Abdul Hakim Abdul Hakim Abdul Qadir Achmad Achmad Aditya Permana Samosir Aditya Permana Samosir Ahmad Ansori Mattjik Ahmad Ansori Mattjik Ahmad Ansori Mattjik Ajmilatunnisa Amin Nur Amin Nur Ani Kurniawati Arifah Rahayu Awang Maharijaya Baiq Arriyadul Badi'ah Cut Tia Mardi dan Nurita Toruan-Mathius Dede Rosyana Budiman Dede Rosyana Budiman Desta Wirnas Dhiea Falihatusy Syarifah Diyah Martanti, Diyah Dwi Yono Edison Jambormias Efrin Firmansyah Eka Bobby Febrianto, Eka Bobby Eka Fatmawati Tihurua Ekasari Putri, Nurwanita Eko Sulistyono Eni Sumarni Erin Puspita Rini Erniati Erniati Eti Rohaeti Fani Fauziah Faqih Udin fatimah Fatimah Folkes Laumal H. M.H. Bintoro Djoefrie H.M.H. BINTORO DJOEFRIE Habibi Firmansah Herdhani, Mira Herry Suhardiyanto Ika Roostika Indriani Nurul Fitri Ireng Darwati Irmanida Batubara Junaedi, Wahyu Jundi Aiman Abdullah Kurniawan, Rizal Mahdi Liana, Devi Linda Novita Mahat Magandhi Mahrup Mahrup, Mahrup Matra, Deden Derajat Mattjik, Ahmad A Maya Melati Memen Surahman Mohamad Solahudin Muhamad Syukur Muhammad Ridha Alfarabi Istiqlal Muhammad Syukur Muhammad Syukur Muhammad Yusuf Pulungan Nadirman Haska Nailan Nabila NFN Sudarsono Nisa, Febianti Khoirun Nugroho, Sasmoyo Adi Nurul Afiyah Otih Rostiana Patty, Jacob R Prawestri, Apriliana Dyah Rizal Mahdi Kurniawan Safitri Nuraina Safitri, Vika Ayu Sasmoyo Adi Nugroho Setiawan, Asep S Shadila Fira Asoka Shalati Febjislami Sintho Wahyuning Ardie Siti Hapshoh Siti Marwiyah Siti Nurhalimah Siti Nurhidayah Slamet Widodo Sobir , Sobir Sobir Sofyan Zaman Sri Gajatri Budiarti Sri Gajatri Budiarti Sri Soenarsih DAS Sri Wardani Sudarmono Sudarsono SUDARSONO SUDARSONO Supriyanto Supriyanto Surjono H Sutjahjo, Surjono H Surjono Hadi Sutjahjo Susi Purwiyanti Suwarno, Punjung Medaraji Tobias Moniz Vicente Tri Handayani Trikoesoemaningtyas Trikoesoemaningtyas Undang Undang Widodo Widodo Willy Bayuardi Suwarno Witjaksono Yuliawati