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All Journal AQUATIC SCIENCE & MANAGEMENT e-Journal BUDIDAYA PERAIRAN JURNAL PESISIR DAN LAUT TROPIS JURNAL ILMIAH PLATAX JURNAL PERIKANAN DAN KELAUTAN TROPIS JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI Briliant: Jurnal Riset dan Konseptual
Veibe Warouw
Fakultas Perikanan dan Ilmu Kelautan, Universitas Sam Ratulangi, Manado, Sulawesi Utara
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science

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Journal : JURNAL ILMIAH PLATAX

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Isolation and Screening the Symbiont Bacteria of the Sponge Dragmacidon sp from Manado Bay, North Sulawesi that Producing Chitinase and Protease Sembiring, Sindiy Cloudya; Warouw, Veibe; Wullur, Stenly; Bara, Robert A; Salaki, Meiske S.; Ginting, Elvy Like
Jurnal Ilmiah Platax Vol. 9 No. 1 (2021): ISSUE JANUARY - JUNE 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.9.1.2021.34307

Abstract

Enzymes are important in the technology industry and hydrolytic enzymes, such as chitinase and protease are commonly used for it. Various types of microorganisms such as bacteria can produce hydrolytic enzymes.  Sponge-associated bacteria are excellent sources of extracellular hydrolytic enzymes because the surface and internal spaces of sponges are richer in nutrients. The aim of this study was to isolate and screen the bacteria of the sponge Dragmacidon sp symbiotic from Manado Bay, North Sulawesi that producing chitinase and protease   Symbiont bacteria were grown in Zobell 1226 E medium with a dilution of 10-4. Bacterial isolation was carried out based on the morphological characteristics of the colony. Chitinase and protease activity was carried out by growing each bacterial isolate in chitin and protein media at 36oC for 48 hours. Chitinase and protease activities were indicated by the formation of a clear zone around the bacterial colony, however, the clear zone for chitinase activity was observed after pouring the Lugol's solution. Based on this study, 8 isolates bacteria of the symbiotic spongy Dragmacidon sp from Manado Bay, North Sulawesi were isolated based on morphological characteristics. The colony of the bacteria is generally white with an irregular shape. Four isolates, namely 1, 2, 3, and 8 had chitinase activity with chitinolytic indexes were 1.7; 1.5; 1.4, and 1.3, respectively. Six isolates, namely 1, 2, 3, 4, 5, and 6 had protease activity with proteolytic indexes were 1.4; 1.8; 3.1; 1.3; 1.8; and 2.5, respectively.Keywords: Bacteria; Chitinolytic; Proteolytic; Symbiont; SpongeAbstrakEnzim menempati posisi penting dalam bidang teknologi dan industri. Enzim yang banyak digunakan dalam bidang industri adalah enzim hidrolase. Enzim dapat diisolasi dari berbagai jenis mikroorganisme seperti bakteri. Bakteri yang berasosiasi dengan spons merupakan sumber enzim hidrolitik ekstraseluler yang sangat baik karena permukaan dan ruang internal spons lebih kaya nutrisi. Penelitian ini bertujuan untuk mengisolasi dan menguji aktivitas kitinase dan protease bakteri simbion spons Dragmacidon sp dari Teluk Manado. Bakteri simbion spons ditumbuhkan dalam media Zobell 1226 E pada pengenceran 10-4. Isolasi bakteri dilaksanakan berdasakan karakteristik morfologi. Aktivitas kitinase dan protease dilaksanakan dengan menumbuhkan setiap isolat bakteri dalam media kitin dan protein pada suhu 36oC selama 48 jam. Aktivitas kitinase dan protease ditandai dengan terbentuknya zona bening di sekitar koloni bakteri yang mana untuk kitinase diamati setelah diberi larutan lugol. Berdasarkan penelitian ini, 8 isolat bakeri simbion spon Dragmacidon sp dari Teluk Manado, Sulawesi Utara berhasil diisolasi berdasarkan karakteristik morfologi. Isolat bakteri umumnya berwarna putih dengan bentuk ireguller. Empat isolat yakni 1, 2, 3, dan 8 memiliki aktivitas kitinase dan enam isolat yakni 1, 2, 3, 4, 5, dan 6 yang memiliki aktivitas protease. Indeks kitinolitik dari masing-masing keempat isolat bakteri secara berturut turut adalah 1,7; 1,5; 1,4; dan 1,3 dengan kategori bernilai rendah dan indeks proteolitik adalah 1,4; 1,8; 3,1; 1,3; 1,8; dan 2,5 dengan kategori bernilai rendah sampai tinggi.Kata kunci: Bakteri; Kitinolitik; Proteolitik; Simbion; Spons
Waste Inventorization Inorganic In The Mangrove Ecosystem Bunaken Island For The East Part Sundah, Geraldo Thimoty; Schaduw, Joshian N. W.; Warouw, Veibe; Kumampung, Deislie R.H.; Paransa, Darus Sa'adah J.; Mokolensang, Jeffrie
Jurnal Ilmiah Platax Vol. 9 No. 2 (2021): ISSUE JULY-DECEMBER 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.9.2.2021.35318

Abstract

The coastal area is a potential resource in Indonesia, is an intermediate area between the mainland and Ocean. This resource is very large which is supported by the existing coastline 81,000 km long. Long coastline This holds the potential for a large wealth of natural resources. The potential including biological and non-biological potential. in addition to the potential for natural resources that are widespread on the coast of Indonesia, potential pollution to the coastal and marine environment has quite a big opportunity. this opportunity could be caused by Indonesia's population density, high tourist activity including transportation, and major construction. As for the goal This study aims to identify the type and amount of waste inorganic in the Bunaken coastal mangrove ecosystem in the eastern part and identify the size and weight characteristics of inorganic waste in the mangrove ecosystem. The method used The result of this research is the coastline survey method methodology based on NOAA (2013) and line transects with taking 2 stations. This research was conducted for three months, which at each station has 1 transect line, each of which has 5 plots/sampling plots. Transect lines are carried out in parallel coastline along 50 meters of trash in the mangrove forest the distance between stations is 50 m, where the transect line must be located represents the research area. The data taken next is back analysis with the help of a computer program MS Excel The types of marine debris found at the research location are plastic, rubber, metal, and glass waste. The total size of the litter type which was found at the research location showed 2 characteristics, namely mega- debris and macro-debris. The most dominant type of waste is plastic waste.Keywords: Inorganic waste, Mangrove, East BunakenAbstrak Wilayah pesisir yang merupakan sumber daya potensial di Indonesia, adalah daerah peralihan antara daratan dan lautan.Sumber daya ini sangat besar yang didukung oleh adanya garis pantai sepanjang sekitar81.000 km. Garis pantai yang panjang ini menyimpan potensi kekayaan sumber alam yang besar.Potensi itu diantaranya potensi non hayati dan hayati.Disamping potensi sumberdaya alam yang tersebar luas di pesisir Indonesia, potensi pencemaran terhadap lingkungan pesisir dan laut pun memilik i peluang yang cukup besar.Peluang ini dapat disebabkan oleh padatnya penduduk Indonesia, aktifitas wis ata yang cukup tinggi termasuk transportasi, dan pembangunan yang besar. Adapun tujuan dari penelitian ini yaitu Mengidentifikasi jenis dan jumlah sampah anorganik yang berada di ekosistem mangrove pantai Bunaken bagian timur Dan Mengidentifikasi karakteristik ukuran dan berat sampah anorganik pada ekosistem mangrove. Metode yang dipakai dalam hasil penelitian ini adalah metode shoreline survey methodology berdasarkan NOAA (2013) dan Line transect dengan mengambil 2 stasiun . Penelitian ini dilaksanakan selama tiga bulan, dimana dalam setiap stasiun terdapat 1 line transect yang masing–masing memilik i 5 petak/plot pengambilan sampel. Jalur transek dilakukukan sejajar garis pantai sepanjang 50 meter adanya sampah pada mangrove jarak antar stasiun adalah 50 m, dimana jalur transek tersebut harus mewakili wilayah penelitian. Data yang di ambil selanjutnya d i analisa kembali dengan bantuan program komputer MS Excel Jenis sampah laut yang ditemukan pada lokasi penelitian berupa sampah plastik, karet, logam, dan kaca. Jumlah ukuran jenis sampah yang terdapat di loksasi penelitian menunjukan bahwa terdapat 2 karakteristik yaitu mega-debris dan macro-debris. Jenis sampah yang paling dominan adalah sampah plastik.Kata Kunci: Sampah Anorganik, Mangrove, Bunaken Timur.
Morphological Characteristics and Shell Color Of Littoraria pallescens Prosobrancia Molusca From Different Mangrove In Tongkaina Waters, Manado City Salawati, Vellysa Friendly; Mantiri, Desy Maria Helena; Boneka, Farnis Bineada; Mamangkey, Noldy Gustaf Frans; Warouw, Veibe; Kalesaran, Ockstan
Jurnal Ilmiah Platax Vol. 10 No. 1 (2022): ISSUE JANUARY-JUNE 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v10i1.38559

Abstract

The purpose of this study was to identify sea slugs L. pallescens taken from two different mangroves, namely Rhizophora mucronata and Avicennia marina in Tongkaina waters, Bunaken District, Manado City based on morphology and anatomy as well as shell color. Identification of mangroves and sea slugs refers to the identification book. The results obtained were L. pallescens species with elongated and tapered morphology at the end of the shell measuring 0.3-2.7 cm. The operculum is purple. The color of the shell obtained was 66.85% consisting of dark colors (black, black, orange, brown and gray spots), occupying the stems and roots of the mangrove, while the light colors (yellow, yellow, dark spots and red) were found to be 33.15%, occupying the leaves and stems of mangroves. The high survival rate of L. pallescens was found in the mangrove roots. This species was found in R. mucronata by 65.26% while in A. marina only 34.74%, this could be caused by differences in the shape of the mangrove roots.Keywords: L. pallescens; Mangrove; Shell color; MorphologyAbstrakTujuan penelitian ini adalah mengindentifikasi siput laut L. pallescens yang diambil dari dua mangrove berbeda yaitu Rhizopora mucronata dan Avicennia marina di perairan Tongkaina, Kecamatan Bunaken, Kota Manado berdasarkan morfologi dan anatomi serta warna cangkang. Identifikasi mangrove dan siput laut merujuk pada buku identifikasi. Hasil penelitian yang diperoleh yaitu spesies L. pallescens dengan bentuk morfologi memanjang dan meruncing, pada bagian ujung cangkang berukuran  0,3-2,7 cm. Operculum berwarna ungu. Warna cangkang yang diperoleh 66,85% yang terdiri dari warna gelap (hitam, hitam bercak orange, coklat dan abu-abu), menempati bagian batang dan akar mangrove sedangkan warna terang (kuning, kuning bercak gelap dan merah) didapat 33,15%, menempati bagian daun dan batang mangrove. Tingginya kelangsungan hidup  L. pallescens berada pada bagian akar mangrove. Spesies ini ditemukan pada R. mucronata sebesar 65,26% sedangkan pada A. marina hanya 34,74%, hal ini dapat disebabkan oleh karena perbedaan bentuk akar mangrove.  Kata Kunci: L. Pallescens; Mangrove; Warna cangkang; Morfologi
Isolation and Antibacterial Activity assay of Endophytic Symbiont Bacteria on Seaweed Gracilaria verrucosa originated from Batu Meja Tongkaina Beach, North Sulawesi Sirri, Yolanda; Warouw, Veibe; Rumengan, Inneke Fenny; Paransa, Darus Sa'adah; Undap, Suzanne Lydia; Ginting, Elvy Like
Jurnal Ilmiah Platax Vol. 10 No. 2 (2022): ISSUE JULY-DECEMBER 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Seaweed can produce bioactive compounds such as antibacterial. Seaweed co-exists with bacteria endophytes. Entophytic bacteria are bacteria that live in host tissues and have the ability to protect the host itself by producing antibacterial compounds against pathogens. Therefore, the endophytic bacteria of seaweed symbionts can be utilized to produce antibacterial compounds. Bacteria can be mass-cultured because of their fast-growing characteristic. The aim of this study was to determine the antibacterial activity from endophytic symbiont bacteria on seaweed Gracilaria verrucosa originated from Batu Meja Tongkaina Beach, North Sulawesi. Isolation of bacteria was carried out by dilution steps from 101 to 10־3 times, then cultivation on Nutrient Agar media to obtain a single colony of the bacteria. Antibacterial activity was tested used paper disc diffusion method. The pathogens used were bacterial strains of Escherichia coli, Staphylococcus aureus, Streptococcus mutans, and Salmonella typhi. Moreover, antibiotics were used as positive controls. The antibacterial ability of the symbiotic bacteria was measured based on inhibition zones around the paper disc containing the isolates of endophytic bacteria. A total of 6 endophytic bacterial isolates were found, namely isolate B, C, D, E, F and G, characterized by different morphological features. The results showed that isolates B and C having inhibition zone of 0.5 – 1.0 mm against S. typhi, indicating that these two isolates produce antibacterial compounds with a weak ability  against S. typhi.
Analysis Of Weather Conditions and Hydrodynamics in Taman Nasional Bunaken Molle, Ben Arther; Schaduw, Joshian Nicolas William; Sumilat, Deiske Adeliene; Rondonuwu, Ari Berty; Luasunaung, Alfret; Warouw, Veibe
Jurnal Ilmiah Platax Vol. 10 No. 2 (2022): ISSUE JULY-DECEMBER 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v10i2.43161

Abstract

Taman Nasional Bunaken (TNB) is located in North Sulawesi Province, Republic of Indonesia. TNB offers an exploration experience of marine tourism such as snorkeling and diving. This study was conducted to determine the statistics and correlation of weather parameters on hydrodynamics in the waters of the small island of TNB. This information can be used by marine tourism users. The weather data studied are wind direction and speed. The hydrodynamic conditions studied were wave height and ocean currents. Respondent surveys were also conducted on tourist users at the research sites. The wind direction for the period January - April is dominant from the Northeast, May - September is dominant from the East to the South, October tends to be evenly distributed from the East to the West, and November - December is dominant from the West. Significant wind speeds that occur in January, February, and March range from 18 – 36 knots. The average wave height shows a significant value in January – March, tends to be lower in April – June, fluctuates in July – November, and increases again in December. The maximum current velocity at a sea depth of 16.5 m is stronger than the maximum speed at a depth of 0.5 m and 8.0 m in January – April, and May August. The correlation value of wind speed and sea wave height is significant at 0.96 and the correlation between wind speed and the current speed is 0.74. These results indicate that there is a strong -very strong relationship between parameters. The survey results show that wind speed, wave height, and ocean currents have the same pattern. January, February, November, and December are the months of extreme or bad weather and hydrodynamic conditions.Keywords: Taman Nasional Bunaken, Weather, Hydrodynamics, CorrelationAbstrakTaman Nasional Bunaken (TNB) terletak di Provinsi Sulawesi Utara, Negara Republik Indonesia. TNB  menawarkan pengalaman eksplorasi suasana wisata bahari seperti snorkling dan diving. Penelitian ini dilakukan untuk mengetahui statistik dan korelasi parameter cuaca terhadap hidrodinamika di perairan pulau kecil TNB. Informasi tersebut dapat digunakan oleh pengguna wisata bahari. Data cuaca yang diteliti adalah arah dan kecepatan angin. Kondisi hidrodinamika yang diteliti ialah tinggi gelombang dan arus laut. Survei responden juga dilakukan kepada pengguna wisata di lokasi penelitian. Arah angin periode Bulan Januari - April dominan dari arah Timur Laut, Bulan Mei – September dominan dari arah Timur hingga Selatan, Bulan Oktober cenderung merata dari arah Timur hingga Barat dan Bulan November – Desember dominan dari arah Barat. Kecepatan angin signifikan terjadi pada bulan Januari, Februari, dan Maret berkisar antara 18 – 36 Knot. Rata-rata tinggi gelombang menunjukan nilai signifikan pada bulan Januari – Maret, cenderung merendah pada bulan April – Juni, berfluktuasi pada bulan Juli – November dan meningkat kembali di bulan Desember. Kecepatan maksimum arus laut di kedalaman 16.5 m lebih kuat jika dibandingkan kecepatan maksimum di kedalaman 0.5 m dan 8.0 m pada bulan Januari – April serta bulan Mei – Agustus. Nilai korelasi kecepatan angin dan tinggi gelombang laut signifikan sebesar 0.96 serta korelasi kecepatan angin dan kecepatan arus sebesar 0.74. Hasil tersebut menunjukkan antar parameter memiliki hubungan yang kuat - sangat kuat. Hasil survei menunjukan kecepatan angin, tinggi gelombang dan arus laut mempunyai pola yang sama. Bulan Januari, Februari, November, dan Desember merupakan bulan-bulan terjadinya kondisi cuaca serta hidrodinamika ekstrem atau buruk.Kata kunci: Taman Nasional Bunaken, Cuaca, Hidrodinamika, Korelasi
Analysis of fishing season for tuna (Thunnus spp.), little tuna (Euthynnus sp.) and skipjack (Katsuwonus pelamis) in FMA 716 Tuyu, Adel M.; Luasunaung, Alfret; Sumilat, Deiske A.; Manoppo, Lefran; Kaparang, Frangky E.; Mantiri, Rose O. S. E.; Warouw, Veibe
Jurnal Ilmiah Platax Vol. 11 No. 1 (2023): ISSUE JANUARY-JUNE 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i1.44633

Abstract

Economically important pelagic fish species in FMA 716 include skipjack (Katsuwonus pelamis), tuna (Thunnus spp.) and frigate tuna (Euthynnus sp.), these pelagic fish species are caught intensively. This study aims to provide information on the appropriate season for catching tuna, skipjack and tuna in FMA 716. The results of the study show the pattern of the tuna, skipjack and frigate tuna (TCT) fishing season based on the catches landed in PPS Bitung and PPP Tumumpa with fishing areas in FMA 716, i.e. the results vary every year but the peak of the fishing season occurs in the eastern transitional season. West - September, October and November. Peak fishing season for skipjack and tuna occurs three times and frigate tuna 2 times in 5 years. Keywords: catching season, Katsuwonus pelamis, Thunnus spp and Euthynnus sp,FMA 716 Abstrak: Jenis ikan pelagis ekonomis penting di WPP 716 antara lain adalah cakalang (Katsuwonus pelamis), tuna (Thunnus spp.) dan tongkol (Euthynnus sp.), jenis – jenis ikan pelagis tersebut ditangkap secara intensif. Penelitian ini bertujuan memberikan informasi musim yang tepat untuk melakukan penangkapan ikan tuna, cakalang dan tongkol di WPP 716. Hasil penelitian menujukan pola musim penangkapan ikan Tuna, Cakalang dan Tongkol (TCT) berdasarkan hasil tangkapan yang didaratkan di PPS Bitung dan PPP Tumumpa dengan wilayah penangkapan di WPP 716 yaitu hasilnya bervariasi setiap tahunnya tetapi yang terbanyak puncak musim penagkapan ikan terjadi pada musim masa peralihan timur-barat yaitu bulan September, Oktober dan November. Puncak musim penangkapan ikan cakalang dan tuna terjadi sebanyak tiga kali dan ikan tongkol 2 kali dalam 5 tahun. Kata kunci : Musim penangkapan ikan tuna, tongkol dan cakalang, WPP 716
Phytoplankton Community Structure in Seagrass Beds in Tiwoho Village, North Minahasa Regency Rimper, Abraham M.; Warouw, Veibe; Rimper, Joice R.T.S.L; Lintang, Rosita A.J.; Ompi, Medy; Pangkey, Henneke D.
Jurnal Ilmiah Platax Vol. 11 No. 1 (2023): ISSUE JANUARY-JUNE 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i1.45232

Abstract

This research was carried out from May to July 2022. A sampling at the study site included seawater for the needs of plankton identification as well as the measurement of water nitrate & phosphate levels and measurement of physical and chemical parameters such as temperature, pH, salinity and dissolved oxygen which were carried out on an ongoing basis In situ (in the field). The technique for determining plankton sampling was carried out purposively, namely with certain considerations to obtain samples that represent the research location area. Plankton sampling was carried out horizontally using a plankton net. Sample bottles containing seawater were identified at the Marine Biology Laboratory, Faculty of Fisheries and Marine Sciences. The composition of the phytoplankton found in the Tiwoho seagrass bed ecosystem consists of three classes, namely the Bacillariophyceae, Dynophyceae, and Cyanophyceae classes, with 28 genera. The results of chopped phytoplankton are expressed in Cells/l. The qualitative determination of plankton is made up to the genus level. The composition of the phytoplankton found in the Tiwoho seagrass bed ecosystem consists of three classes, namely the Bacillariophyceae, Dynophyceae, and Cyanophyceae classes, with 28 genera. The results of calculating the abundance of phytoplankton in the Tiwoho seagrass ecosystem are in the range of 29 - 66 cells/l. The highest abundance was found at station two, namely 66 cells/l with 21 genera, then station three (65 cells/l) with 24 genera, and station one (29 cells/l) with a total of 23 genera. The diversity index of seagrass beds in Tiwoho waters is in the range of 1.9330 - 2.4083, which means that community stability is categorized as moderate. The uniformity index is in the range of 0.4614 - 0.7154, this means that the uniformity between species is relatively the same, or the difference is not striking. While the domination index is in the range of 0.1494 - 0.2404, it means that the condition of the community structure is stable, and there is no ecological pressure (stress) on biota. Keywords: Phytoplankton, Biological Index, Abundance, Environmental parameters Abstrak Penelitian ini dilaksanakan pada bulan Mei sampai bulan Juli 2022. Pengambilan sampel di lokasi penelitian meliputi air laut untuk kebutuhan identifikasi fitoplankton dan pengukuran kadar nitrat, fosfat perairan serta pengukuran parameter fisika kimia seperti suhu, pH, salinitas dan oksigen terlarut yang dilakukan secara In situ (di lapangan). Teknik penentuan pengambilan sampel plankton dilakukan secara purposif yaitu dengan pertimbangan tertentu untuk mendapatkan sampel yang mewakili area lokasi penelitian. Pengambilan sampel plankton dilakukan secara horisontal dengan menggunakan plankton net. Sampel plankton diberi pengawet formalin 4% dan diidentifikasi di laboratorium Biologi Kelautan Fakultas Perikanan dan Ilmu Kelautan. Komposisi fitoplankton yang dijumpai di ekosistem padang lamun Perairan Tiwoho terdiri dari tiga kelas yaitu kelas Bacillariophyceae, Dynophyceae, dan Cyanophyceae, dengan 28 genera. Hasil perhitungan kelimpahan fitoplankton di ekosistem padang lamun Perairan Tiwoho berada di kisaran 29 - 66 sel/l. Kelimpahan tertinggi ditemukan di stasiun dua (depan kampung) yaitu 66 sel/l dengan 21 genera, kemudian stasiun tiga (depan dermaga) yaitu 65 sel/l dengan 24 genera, dan stasiun satu (ujung kampung) sebanyak 29 sel/l dengan jumlah 23 genera. Indeks keanekaragaman ekosistem padang lamun Perairan Tiwoho berada pada kisaran 1,9330 - 2,4083, yang berarti stabilitas komunitas dikategorikan sedang. Indeks keseragaman yaitu pada kisaran 0,4614 - 0,7154, ini berarti keseragaman antar spesies relatif sama, atau perbedaannya tidak menyolok. Sedangkan indeks dominasi yaitu pada kisaran 0,1494 - 0,2404, berarti kondisi struktur komunitas dalam keadaan stabil, dan tidak terjadi tekanan ekologi (stress) terhadap biota. Kata kunci: Fitoplankton, Indeks Biologi, Kelimpahan, Parameter lingkungan
Anti-Bacterial And Anti-Ultraviolet Activity Test Of Black Cucumber (Holothuria atra) Extract From Tongkaina Waters, Bunaken District, Manado City Mangangkung, Nazarrian; Angkouw, Esther Dellayani; Warouw, Veibe; Mangindaan, Remy Emile Petrus; Losung, Fitje; Monijung, Revol Dulles
Jurnal Ilmiah Platax Vol. 11 No. 2 (2023): ISSUE JULY-DECEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i2.48018

Abstract

          The purpose of this study was to obtain the crude extract of Holothuria atra, test its antibacterial activity against Escherichia coli and Bacillus megaterium strains using the disc diffusion method (Kirby-Bauer disc diffusion), test the anti-UV of Holothuria atra extract taken from Tongkaina Waters, Bunaken District, Manado City. Antibacterial testing was carried out with several different concentrations, from the results of antibacterial testing carried out the average inhibition zone on E. coli bacteria with 100,000 ppm the average inhibition zone was (9.67 mm), for 50,000 ppm the average inhibition zone was (8 mm), from B. megaterium bacteria known to have H.a 100,000 ppm producing an average inhibition zone (9.67 mm), H.a 50,000 ppm an average inhibition zone of (8.67 mm). The results of the anti-UV test for Holothuria atra extract showed absorption in UV-C at λ 210 nm with the highest absorbance value of 2.774. Keywords: Sea cucumber, Antibacterial, Anti-UV, Escherichia coli, Bacillus megaterium. Abstrak Tujuan dari penelitian ini untuk mendapatkan ekstrak kasar Holothuria atra, menguji aktivitas antibakteri terhadap strain Escherichia coli dan Bacillus megaterium dengan metode difusi agar (disc diffusion Kirby-Bauer), menguji anti-UV dari ekstrak Holothuria atra yang diambil dari Perairan Tongkaina Kecamatan Bunaken Kota Manado. Pengujian antibakteri dilakukan dengan beberapa konsentrasi yang berbeda, dari hasil pengujian antibakteri yang dilakukan rerata zona hambat pada bakteri E. coli dengan 100.000 ppm rerata zona hambat sebesar (9,67 mm), untuk 50.000 ppm rerata zona hambat sebesar (8 mm), dari bakteri B. Megaterium diketahui H.a 100.000 ppm menghasilkan rerata zona hambat (9,67 mm), H.a 50.000 ppm rerata zona hambat sebesar (8,67 mm). Untuk hasil pengujian anti-UV ekstrak Holothuria atra menunjukkan serapan pada UV-C pada λ 210 nm dengan nilai absorban tertinggi 2,774. Kata kunci : Teripang laut, antibakteri, anti-UV, Escherichia coli, Bacillus megaterium.
Zooplankton in the Seagrass Beds of Nain Island Tuliabu, Nelda; Rimper, Joice R.T.S.L; Warouw, Veibe; Kaligis, Erly Yosef; Rumampuk, Natalie Detty C; Ngangi, Edwin Leonardo Apolonio
Jurnal Ilmiah Platax Vol. 12 No. 1 (2024): ISSUE JANUARY-JUNE 2024
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v12i1.50187

Abstract

This research was conducted in July 2022, the determination of sampling locations was carried out by purposive sampling, namely data collection with certain considerations to obtain samples that represent the research location area. Seawater samples were taken using a plankton net which was then used for the zooplankton identification process carried out in the Marine Biology Laboratory of the Faculty of Fisheries and Marine Science. Measurement of physical parameters of water chemistry consisting of temperature, salinity, and pH, was carried out in situ. The content of nitrate and phosphate was conducted at the WLN laboratory (Water Laboratory Nusantara-WLN). Zooplankton counts were expressed in ind/l. Qualitative determination of plankton was made up to the genus level. The results of zooplankton identification consisted of 5 genera namely Acartia, Cyclops, Euterpina, Nauplius, and Oithona. The calculation of zooplankton abundance is in the range of 3-5 ind/l. The highest abundance was found at station one which was 5 ind/l followed by station three which was 4 ind/l and then station two as much as 3 ind/l. The diversity index is in the range of 0.4740-0.7786, the uniformity index is in the range of 0.2945-0.7087, and the dominance index ranges from 0.5971-0.7744. Keywords: Zooplankton, Seagrass Meadow, Nain Island, Abundance, Biological Index. Abstrak Penelitian ini dilaksanakan pada bulan Juli 2022, penentuan lokasi pengambilan sampel dilakukan dengan cara purposive sampling yaitu pengumpulan data dengan pertimbangan tertentu untuk memperoleh sampel yang mewakili wilayah lokasi penelitian. Sampel air laut diambil dengan menggunakan plankton net yang selanjutnya digunakan untuk proses identifikasi zooplankton yang dilakukan di Laboratorium Biologi Kelautan Fakultas Perikanan dan Ilmu Kelautan. Pengukuran parameter fisik kimia air yang terdiri dari suhu, salinitas, pH, dilakukan secara in situ. Kadar nitrat dan fosfat dilakukan di laboratorium WLN (Laboratorium Air Nusantara-WLN). Jumlah Zooplankton dinyatakan dalam ind/l. Penentuan plankton secara kualitatif dilakukan sampai tingkat genus. Hasil identifikasi zooplankton terdiri dari 5 genus yaitu Acartia, Cyclops, Euterpina, Nauplius, dan Oithona. Perhitungan kelimpahan zooplankton berada pada kisaran 3-5 ind/l. Kelimpahan tertinggi terdapat pada stasiun satu sebesar 5 ind/l, disusul stasiun tiga sebesar 4 ind/l dan kemudian stasiun dua sebanyak 3 ind/l. Indeks keanekaragaman berada pada rentang 0,4740-0,7786, indeks keseragaman berada pada rentang 0,2945-0,7087, sedangkan indeks dominasi berkisar antara 0,5971-0,7744. Kata Kunci: Zooplankton, Padang Lamun, Pulau Nain, Kelimpahan, Indeks Biologi.
Attachment Of Macrobenthos Larvae To Organic And Non-Organic Substrates Saragih, Hans S. R. P.; Ompi, Medy; Kaligis, Erly Yosef; Boneka, Farnis B. Boneka; Warouw, Veibe; Paransa, Darus Sa’adah Johanis
Jurnal Ilmiah Platax Vol. 12 No. 1 (2024): ISSUE JANUARY-JUNE 2024
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v12i1.52205

Abstract

The objectives of this study are 1) to Identify the specimen of macrobenthos attached to the substrate provided in 2 locations. 2) to determine the type of substrate attached by the larvae at both sites.  3) to determine the density of Macrobenthos attached to the substrate provided at 2 sites. A plywood plate had 16 holes with a diameter of 1 cm each that had been randomly filled with organic substrates, namely coconut fibers, palm fibers, shells with 'bysus' threads, and non-organic substrates in the form of plastic ropes. Each substrate has four replicates.  The plywood plate with 3 replicates was placed in each station.  The plate was removed and taken to the laboratory after 1 month. The identification up to family, genus, and species as well as the density of each species were applied.  The results showed that not only the larvae of Septifer bilocularis attached to organic and non-organic substrates, but six species of larvae were also identified. The sizes of new settler macrobenthos from 2 mm to 1 cm attached on organic and non-organic substrates were identified. The density of new settlers species attached to substrates varied from 0.03 – 0.5 individuals/cm2.  The new settlers identified 7 species in Tiwoho and 3 species in Malalayang.    Keywords: Settlement, Substrate, Tiwoho Coast, Malalayang Coas. Abstrak Tujuan dari penelitian ini adalah 1) Mengidentifikasi jenis-jenis  makrobenthos yang menempel pada substrat yang disediakan di 2 lokasi. 2) Mengidentifikasi jenis substrat sebagai tempat penempelan larva macrobenthos di  kedua lokasi, dan 3) Menentukan kepadatan jenis Makrobenthos  pada substrat yang disediakan di 2 lokasi. Triplek (plate) memiliki 16 lubang dengan ukuran diameter masing-masing 1 cm yang telah diisi secara acak dengan substrat organik yaitu serabut kelapa, serabut ijuk, cangkang ber ‘byssus’, serta substrat non organik berupa tali plastik. Masing-masing substrat ini memiliki 4 ulangan.  Selanjutnya, plate, masing-masing dengan 3 ulangan ditempatkan di setiap intertidal, Towoho dan Malalayang.  Plate diangkat setelah 1 bulan, yang dibawah ke laboratorium untuk foto dan diidentifikasi baik di tingkat jenis, genus, ataupun family.  Hasil penelitian menunjukkan bahwa tidak hanya larva kerang Septifer bilocularis (Linnaeus, 1758), yang menempel pada substrat organik dan non organik, tetapi ditemukan larva macrobenthos lainnya, yaitu 2 jenis dari Cerithum egenum (Gould, 1849), dan Calcarina defranci d'Orbigny, 1826, 2 family/genus, yaitu: Canthocamptidae dan Portunidae, dan 2 klass, yaitu : Polychaeta dan Demospongae.  Teridentifikasi jenis larva makro benthos yang baru menempel pada substrat substrat organik dan non-organik dengan ukuran yang bervariasi, yaitu dari 2 mm – 1 cm. Kepadatan jenis macrobenthos yang baru menempel adalah dari 0.03 – 0.5 individu/cm2.  Ada 7 jenis teridentifkasi di lokasi Tiwoho, dan 3 jenis teridentifkasi di Malalayang Kata kunci: Penempelan, Substrat, Pesisir Tiwoho, Pesisir Malalayang  
Co-Authors Alfret Luasunaung Angkouw, Esther Dellayani Angmalisang, Ping Astony Antonius Rumengan Ari Berty Rondonuwu Boneka, Farnis B. Boneka Boneka, Farnis Bineada Calvyn F. A. Sondak, Calvyn F. A. Carolus Paulus Paruntu Cyska Lumenta Darus S. Paransa Deiske Adeliene Sumilat, Deiske Adeliene Elvy L Ginting Farnis B. Boneka Fitje F. Losung, Fitje F. Fitje Losung Frangky E. Kaparang Ginting, Elvy Like Henky Manoppo Henneke Pangkey Hidayat, Andre Alfian Indri Manembu Janny D. Kusen Johnny Budiman Joice R.T.S.L Rimper Joshian N.W. Schaduw Kalebos, Roosa C. Kaligis, Erly Yosef Kreckhoff, Reni L. Kumampung, Deislie R.H. Kusumaningrum, Arianda Lasut, Astrid Y. Lindon R Pane Lintang, Rosita A.J. Lumingas, Lawrence L.J. Mahmud, Maudy Rusmini Mamangkey, Noldy Gustaf Frans Mamuaja, Jane M. Mangangkung, Nazarrian Mangindaan, Remy Mangindaan, Remy Emile Petrus Manoppo, Lefran Mantiri, Desy M. H Maramis, Regina U. Markus T. Lasut Medy Ompi Mokolensang, Jeffrie Mokolensang, Jeffrie F. Mokosuli, Febrianty Dhea Molle, Ben Arther Monijung, Revol Dulles Natalie D Rumampuk Ngangi, Edwin Leonardo Apolonio Nowin, Edgar Ockstan J. Kalesaran Ockstan Kalesaran Pane, Lindon R. Pangalila, Novelia M.A. Pangkey, Henneke D. Paransa, Darus Sa'adah Paransa, Darus Sa'adah Johanis Paransa, Darus Sa’adah Johanis Paulus, James Pesoth, Christianto R. T. D. Maramis Ramadan, Febrian Reiny A. Tumbol Rimper, Abraham M. Rimper, Joice R. T. S. L . Rizal W Suleman Robert A. Bara Roeroe, Kakaskasen Andreas Rose O. S. E. Mantiri, Rose O. S. E. Rumampuk, Natalie D.C Rumampuk, Natalie Detty C Rumampuk, Natalie Detty C. Rumengan, Inneke Fenny Salaki, Christina Leta Salaki, Meiske S. Salawati, Vellysa Friendly Saragih, Hans S. R. P. Sembiring, Sindiy Cloudya Silvana Dinaintang Harikedua Silvester B Pratasik Sirri, Yolanda Stenly Wullur sukmawati Sumual, Sarah S. Sundah, Geraldo Thimoty Tuliabu, Nelda Tuyu, Adel M. Undap, Suzanne J Undap, Suzanne Lydia Wilhelmina Patty Wilmy E Pelle