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Journal : JFMR (Journal of Fisheries and Marine Research)

THE EFFECT OF CRUDE PROTEIN HALIMEDA SP. ON CYPRINUS CARPIO INFECTED KOI HERPES VIRUS ON EXPRESSION OF MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-1 Uun Yanuhar; Rika Wahyuningtyas
JFMR (Journal of Fisheries and Marine Research) Vol 1, No 1 (2017): JFMR VOL 1 NO 1
Publisher : JFMR (Journal of Fisheries and Marine Research)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.567 KB) | DOI: 10.21776/ub.jfmr.2017.001.01.3

Abstract

Halimeda sp. is a kind species of macroalgae that abundant grow in Indonesia. The utilization of Halimeda especially protein for disease prevention caused by the virus has not been done to the fish. The purpose of the study is to know the  treatment of crude protein Halimeda sp on  Cyprinus carpio infected by Koi Harvest Virus (KHV) on the expression of Major Histocompatibility Complex (MHC) class 1. MHC class-1 is one of the immune adaptive response on fish like this C. carpio. The methods are isolation of crude protein Halimeda sp,   purification  of  crude protein, Haemagglutination (HA) test and dot blot test. The result shows that the crude protein of Halimeda sp. can produce of adaptive immune response like MHC class 1. MHC class-1 has the function of  the immune system for maintenance of virus attacks directly. Responsible of treatment crude protein Halimeda sp. on C. carpio can be showed quantitatively using the dot blot test result. The  conclusion  is the crude protein of Halimeda sp. able to activate the MHC class I and to inhibit the proliferation of the KHV and maintenance of the fish cell of C. carpio..
N-VIVO TEST OF Chlorella vulgaris EXTRACT AS HEAT SHOCK PROTEINS INDUCTION OF CANTANG GROUPER (Ephinephelus fuscoguttatus-lanceolatus) INFECTED BY VIRAL NERVOUS NECROSIS Amira Masitha; Uun Yanuhar; Asus Maizar Suryanto Hertika
JFMR (Journal of Fisheries and Marine Research) Vol 3, No 1 (2019): JFMR VOL 3 NO.1
Publisher : JFMR (Journal of Fisheries and Marine Research)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.14 KB) | DOI: 10.21776/ub.jfmr.2019.003.01.3

Abstract

Cantang Grouper fish (Ephinephelus fuscoguttatus-lanceolatus) has a high value both in international and local markets. However, these fishes have decreased production due to viral infection of VNN (Viral Nervous Necrosis). In relation to viral infections, heat shock protein (HSP) inducer derived from C. vulgaris is an effort to be used as an anti-inflammatory agent due to VNN infection. The aim of this research to know the potential of C. vulgaris extracts as an inducer of heat shock protein. The method used in this research is experimental method using in-vivo test treatment of C.vulgaris extracts with of 33 μg/ml in Cantang grouper fish with four treatments that are (A) control fish, (B) fish infected with VNN, (C) fish treated with C. vulgaris extracts, and (D) Fish treated with C. vulgaris extracts  and VNN infection. The results showed that in the phytochemical test, C. vulgaris extracts showed that there are three compounds contained are alkaloids, terpenoids, and tannins. Control fish was labelled using IgG anti Hsp 70 anti-mouse inflammatory response no apparent inflammatory reaction, fish treated with  33 μg/ml of C. vulgaris extracts HSP-70 responses was low inflammatory response, and fish treated with  33 μg/ml of C. vulgaris extract and VNN inflammatory response strengthened and tissue inflammatory response only with VNN infection were very strong. The results showed the treatment of 33 μg/ml of C. vulgaris extracts can inhibit the development of the virus also capable reduced the inflammatory response to Cantang grouper infected by VNN.
In-Vivo Test of Spirulina sp as Inducer of β-Actin In Cantang Grouper (Epinephelus fuscoguttatus-lanceolatus) Infected by Viral Nervous Necrosis Yovan Endik Irawanto; Uun Yanuhar; Andi Kurniawan
JFMR (Journal of Fisheries and Marine Research) Vol 2, No 3 (2018): JFMR VOL 2 NO 3
Publisher : JFMR (Journal of Fisheries and Marine Research)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (517.566 KB) | DOI: 10.21776/ub.jfmr.2018.002.03.11

Abstract

Cantang grouper fish (Epinephelus fuscoguttatus-lanceolatus) is a result of hybridized fish between a female of tiger grouper fish (Epinephelus fuscoguttatus) and male of kertang grouper (Ephinephelus lanceolatus). In the development of cultivation, there are many problems, one of them infected with the class of virus Nodaviridae, namely Viral Nervous Necrosis (VNN). Fish had a defense against cellular immunity against the VNN virus by β-actin. The aims of this research to explore the crude extract of Spirulina sp as a β-actin inducer for the anti-inflammatory immune system in grouper fish against VNN attack. The method used in this research is experiment methods.  Crude extracts of Spirulina sp (33 μg/ml) were conducted by feeding orally to groupers, and VNN infections were conducted by feeding the already positive VNN meat. Detection of VNN using RT-PCR, however β-actin detection using PCR, and IHC in the organ of Cantang grouper fish. The results showed that the percentage of DAB value of control fish (14.0%), fish treated with Spirulina sp (25.7%), fish treated with VNN (31.9%), and fish treated with Spirulina sp extract and VNN infection (32.4%). The percentage of DAB values were indicated by the detection of the target gene β-actin. Immunity in fish increases with the addition of Spirulina sp. The increased β-actin expression may also be used as an indicator of a grouper's body defense against VNN infection.
Komposisi dan Dinamika Sampah Laut di Kawasan Ekowisata Mangrove Wonorejo, Surabaya: Composition and Dynamics of Marine Debris in the Wonorejo Mangrove Ecotourism Area, Surabaya Isdianto, Andik; Yanuhar, Uun; Lelono, Tri Djoko; Susilo, Edi; Ramadhanti, Nur Fa'izah Fitri; Nizery, Sefhanissa Puspa Retno; Fathah, Aulia Lanudia; Batoro, Jati; Putri, Berlania Mahardika; Wardana, Novar Kurnia; Puspitasari, Intan Dwi
JFMR (Journal of Fisheries and Marine Research) Vol. 8 No. 3 (2024): JFMR on November
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2024.008.03.12

Abstract

Penelitian ini bertujuan untuk mengkaji pengaruh muson dan arus laut terhadap distribusi dan komposisi sampah di kawasan Ekowisata Mangrove Wonorejo, Surabaya. Dengan menggunakan metode survei garis pantai di lima lokasi penelitian, data sampah dikumpulkan pada Maret, Mei, dan September 2024. Analisis terfokus pada pengukuran jumlah dan berat sampah terutama plastik, karet, kain, dan bahan lainnya. Hasil menunjukkan bahwa sampah plastik adalah yang paling dominan, mencapai 95,33% dari total berat sampah pada September, sedangkan pada bulan Maret dan Mei, jumlahnya juga sangat tinggi. Lonjakan signifikan dalam sampah karet tercatat pada Mei, kemungkinan disebabkan oleh kegiatan rekreasi yang meningkat selama musim liburan. Pola perubahan sampah menunjukkan bahwa muson barat (Desember-Maret) yang membawa curah hujan tinggi menyebabkan peningkatan akumulasi sampah di pantai, terutama plastik dan karet, karena aliran permukaan yang membawa sampah dari daratan ke pesisir. Sebaliknya, pada muson timur (Juni-September), yang umumnya lebih kering, terjadi penurunan jumlah sampah yang terakumulasi, terutama plastik, karena arus laut yang bergerak menjauh dari pantai mengurangi penumpukan sampah di pesisir. Mangrove berperan penting sebagai filter alami, menangkap dan mengurangi sampah yang masuk ke ekosistem pesisir. Berdasarkan temuan ini, disarankan peningkatan fasilitas pengelolaan sampah dan edukasi publik tentang pengurangan penggunaan plastik sekali pakai untuk mengurangi dampak negatif sampah pada ekosistem mangrove.   This study was conducted to understand the influence of monsoons and ocean currents on the distribution and composition of waste in the Wonorejo Mangrove Ecotourism, Surabaya. Using a shoreline survey method at five locations, waste data was collected in March, May, and September 2024. The analysis focused on measuring the amount and weight of waste, especially plastic, rubber, cloth, and other materials. The results showed that plastic waste was the most dominant, reaching 95.33% of the total weight of waste in September, while in March and May, the amount was also very high. A significant spike in rubber waste was recorded in May, possibly due to increased recreational activities during the holiday season. The pattern of waste changes showed that the west monsoon (December-March) which brought high rainfall caused an increase in the accumulation of waste on the beach, especially plastic and rubber, due to surface flow carrying waste from the mainland to the coast. Conversely, in the east monsoon (June-September), which is generally drier, there was a decrease in the amount of accumulated waste, especially plastic, because the ocean current moving away from the coast reduced the accumulation of waste on the coast. Mangroves play an important role as natural filters, capturing and reducing waste that enters the coastal ecosystem. Based on these findings, it is recommended to improve waste management facilities and public education on reducing the use of single-use plastics to reduce the negative impact of waste on the mangrove ecosystem.
THE EFFECT OF CRUDE PROTEIN HALIMEDA SP. ON CYPRINUS CARPIO INFECTED KOI HERPES VIRUS ON EXPRESSION OF MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-1 Yanuhar, Uun; Wahyuningtyas, Rika
JFMR (Journal of Fisheries and Marine Research) Vol. 1 No. 1 (2017): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2017.001.01.3

Abstract

Halimeda sp. is a kind species of macroalgae that abundant grow in Indonesia. The utilization of Halimeda especially protein for disease prevention caused by the virus has not been done to the fish. The purpose of the study is to know the  treatment of crude protein Halimeda sp on  Cyprinus carpio infected by Koi Harvest Virus (KHV) on the expression of Major Histocompatibility Complex (MHC) class 1. MHC class-1 is one of the immune adaptive response on fish like this C. carpio. The methods are isolation of crude protein Halimeda sp,   purification  of  crude protein, Haemagglutination (HA) test and dot blot test. The result shows that the crude protein of Halimeda sp. can produce of adaptive immune response like MHC class 1. MHC class-1 has the function of  the immune system for maintenance of virus attacks directly. Responsible of treatment crude protein Halimeda sp. on C. carpio can be showed quantitatively using the dot blot test result. The  conclusion  is the crude protein of Halimeda sp. able to activate the MHC class I and to inhibit the proliferation of the KHV and maintenance of the fish cell of C. carpio..
N-VIVO TEST OF Chlorella vulgaris EXTRACT AS HEAT SHOCK PROTEINS INDUCTION OF CANTANG GROUPER (Ephinephelus fuscoguttatus-lanceolatus) INFECTED BY VIRAL NERVOUS NECROSIS Masitha, Amira; Yanuhar, Uun; Hertika, Asus Maizar Suryanto
JFMR (Journal of Fisheries and Marine Research) Vol. 3 No. 1 (2019): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2019.003.01.3

Abstract

Cantang Grouper fish (Ephinephelus fuscoguttatus-lanceolatus) has a high value both in international and local markets. However, these fishes have decreased production due to viral infection of VNN (Viral Nervous Necrosis). In relation to viral infections, heat shock protein (HSP) inducer derived from C. vulgaris is an effort to be used as an anti-inflammatory agent due to VNN infection. The aim of this research to know the potential of C. vulgaris extracts as an inducer of heat shock protein. The method used in this research is experimental method using in-vivo test treatment of C.vulgaris extracts with of 33 μg/ml in Cantang grouper fish with four treatments that are (A) control fish, (B) fish infected with VNN, (C) fish treated with C. vulgaris extracts, and (D) Fish treated with C. vulgaris extracts  and VNN infection. The results showed that in the phytochemical test, C. vulgaris extracts showed that there are three compounds contained are alkaloids, terpenoids, and tannins. Control fish was labelled using IgG anti Hsp 70 anti-mouse inflammatory response no apparent inflammatory reaction, fish treated with  33 μg/ml of C. vulgaris extracts HSP-70 responses was low inflammatory response, and fish treated with  33 μg/ml of C. vulgaris extract and VNN inflammatory response strengthened and tissue inflammatory response only with VNN infection were very strong. The results showed the treatment of 33 μg/ml of C. vulgaris extracts can inhibit the development of the virus also capable reduced the inflammatory response to Cantang grouper infected by VNN.
In-Vivo Test of Spirulina sp as Inducer of β-Actin In Cantang Grouper (Epinephelus fuscoguttatus-lanceolatus) Infected by Viral Nervous Necrosis Irawanto, Yovan Endik; Yanuhar, Uun; Kurniawan, Andi
JFMR (Journal of Fisheries and Marine Research) Vol. 2 No. 3 (2018): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2018.002.03.11

Abstract

Cantang grouper fish (Epinephelus fuscoguttatus-lanceolatus) is a result of hybridized fish between a female of tiger grouper fish (Epinephelus fuscoguttatus) and male of kertang grouper (Ephinephelus lanceolatus). In the development of cultivation, there are many problems, one of them infected with the class of virus Nodaviridae, namely Viral Nervous Necrosis (VNN). Fish had a defense against cellular immunity against the VNN virus by β-actin. The aims of this research to explore the crude extract of Spirulina sp as a β-actin inducer for the anti-inflammatory immune system in grouper fish against VNN attack. The method used in this research is experiment methods.  Crude extracts of Spirulina sp (33 μg/ml) were conducted by feeding orally to groupers, and VNN infections were conducted by feeding the already positive VNN meat. Detection of VNN using RT-PCR, however β-actin detection using PCR, and IHC in the organ of Cantang grouper fish. The results showed that the percentage of DAB value of control fish (14.0%), fish treated with Spirulina sp (25.7%), fish treated with VNN (31.9%), and fish treated with Spirulina sp extract and VNN infection (32.4%). The percentage of DAB values were indicated by the detection of the target gene β-actin. Immunity in fish increases with the addition of Spirulina sp. The increased β-actin expression may also be used as an indicator of a grouper's body defense against VNN infection.
Co-Authors Abdul Rahem Faqih Ach. Khumaidi Adelya Desi Kurniawati Adhitya, Dewa Sukma Trinanda Agus Susilo Alfarisi, Muhammad Asnin Amasda, Naufal Rizky Aminnudin, Aminnudin Amira Baihani Amira Baihani Amira Masitha Anasiru, Arief Farhan Kurniawan Andi Kurniawan Andik Isdianto Anggraini, Rifka Rimbi Anisa Millah Taqiyyah Anjasmara, Aang Setyawan Anni Faridah Annisa Fitri Anggreini Apri Supii Asep Awaludin Prihanto Asus Maizar Suryanto H Asus Maizar Suryanto Hertika Awalia Oktaviana Bili Darnanto Susilo Binoj, Joseph Selvi BS, Sumitro Choirul Huda Choirul Huda Cucun Herlina Daimon Syukri Diana Arfiati Dwi Retna Kumalaningrum Dyah Kinasih Wuragil Dyah Kinasih Wuragil Dyah Kinasih Wuragil Dyah Tri Rahayu Dyah Wuragil E Ernawati, E Eddy Suprayitno Edi Susilo Endang Yuli Herawati Fadjar, Mohammad Fajar Shodiq Fathah, Aulia Lanudia Fauliza, Tifa feri Setiawan Fika Fitrianesia Fitri, Kharisma Nuria Fitrianesia, Fika Gatot Ciptadi Gede Angga Krishna Fariestha Gian Suryanatha Hartawan Gian Suryanatha Hartawan Gunanti Mahasri Hardoko Hardoko Herlina, Cucun Herly Evanuarini Herly Evanuarini Herly Evanuarini Heru Suryanto Immaria Fransira Indah Wahyuni Abida Irawanto, Yovan Endik Ismail, Mahrus Ita Fitria S Jati Batoro Jibril Maulana Komarudin Komarudin MA, Widodo Maftuch ., Maftuch Maftuch Maftuch Maheno Sri Widodo, Maheno Sri Maria Kristiani Marsono Marsono Martiningsih, Nur Fauzi-yah Martiningsih, Nur Fauziyah Masitha, Amira Mohammad Bisri Mohammad Fadjar Mohammad Musa Mufidah, Rhobithotus Muhamad Muhajir Muhammad Faiq Ash Shiddiq Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Sumsanto Muhammad Sumsanto Musa, Mohammad Nico Caesar Nico Rahman Caesar Nizery, Sefhanissa Puspa Retno Nur Fauziyah Martiningsih Nur Junirahma Nur Sakinah Junirahma Nusantara, Fajar Osman, Azlin Fazlina Pahlefi, Reval Pradana, Yanuar Rohmat Aji Purwoko, Agus Puspitasari, Intan Dwi Putri, Berlania Mahardika R Adharyan Islamy Ramadhanti, Nur Fa'izah Fitri Rifka Rimbi Anggraini Rika Wahyuningtyas Rika Wahyuningtyas Rika Wahyuningtyas, Rika Rizka Rahmana Putri Sanoesi, Ellana Sansi, Delfina Husniya Septi Anitasari, Septi Setyayunita, Tamaryska Shiddiq, Muhammad Faiq Ash Sias, Quota Alief Soelistyoadi, Rachmat Noer Soemarno Soemarno Sri Andayani Sri Andayani Sri Andayani Andayani Sri Rahmaningsih Sri Rahmaningsih Sukarni Sukarni Suprayitno Suprayitno Susilowati, Surya Rachman Taufik Budhi Pramono Taufik Budhi Pramono Teotonio Gomes, Guilherme Tri Djoko Lelono Ulhaq, Ulfieda Anwar Utari, Inpita Casuarina Eqisetia Wardani, Nezya Pramudya Wuragil, Dyah Kinasih Yenny Risjani Yovan Endik Irawanto Yusuf Arif Wahyudi Yusuf Arif Wahyudi Yusuf Wahyudi