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Journal : Research Journal of Life Science

Analysis of The Secondary Metabolite of Kersen Leaf Extracts (Muntingia calabura L.) and Its Potential as Anti-Bacteria to Inhibit Aeromonas hydrophila Gede Angga Krishna Fariestha; Sri Andayani; Uun Yanuhar
Research Journal of Life Science Vol 5, No 2 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (301.487 KB) | DOI: 10.21776/ub.rjls.2018.005.02.6

Abstract

Aeromonas hydrophila is a gram-negative bacterium that massively attack aquatic organisms in fresh water, briny water and sea waters. Infection due to this bacterium caused septicemia in the host's body to the point of damaging the body's organs. Hence, alternative material is required in order to cope with this bacterium, which was by using natural material such as kersen leaf (Muntingia calabura L). The purpose of the research is to determine the effect of secondary metabolite contained in kersen leaf as anti-bacterial against Aeromonas hydrophila. This research used a method of MIC (Minimum Inhibitory Concentration) as an anti-bacterial test. In order to examine the secondary metabolite content, phytochemical screening and FTIR (Fourier Transform Infrared Spectroscopy) methods were used. The results obtained through the MIC test of 125 ppm is the minimum concentration capable of inhibiting the growth of Aeromonas hydrophila. Kersen leaf extracts is bacteriostatic anti-bacteria which only inhibit bacterial growth up to 24 hours. Phytochemical screening reported that kersen leaf extracts were positive of flavonoids, tannins, saponins, alkaloids and triterpenoids. Through FTIR test, it is known that phenol compounds were contained in kersen leaf extracts. This phenol is one of the metabolites with the function of anti-bacterial on this study.
Antibacterial Activity of Dayak Onion Bulbs (Eleutherine palmifolia (L) Merr) Ethanol Fraction against Pseudomonas fluorescens and Its Secondary Metabolite Analysis Immaria Fransira; Annisa Fitri Anggreini; Uun Yanuhar; Maftuch Maftuch
Research Journal of Life Science Vol 6, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (487.206 KB) | DOI: 10.21776/ub.rjls.2019.006.02.3

Abstract

Pseudomonas fluorescens is a gram-negative pathogenic bacteria which is one of the problems in fish farming that can causes death in fish. The alternative to overcome this bacterial attack is by using of natural material such as dayak onion bulbs (Eleutherine palmifolia (L) Merr), which are known to have secondary metabolites. The purpose of this research was to determine the antibacterial activity against P. flourescens that contained in the dayak onion bulbs (E. palmifolia (L) Merr) ethanol fraction and the content of antibacterial compounds from the best dayak onion bulbs (E. palmifolia (L) Merr) ethanol fraction. The method used in this research was antibacterial test with MIC (Minimum Inhibitory Concentration) followed by disc test and FTIR (Fourier Transform Infrared Spectroscopy) to analysis secondary metabolite. Fractionation was conducted using Chloroform: Methanol (9:1 v/v). MIC test result showed that 100 ppm was the minimum concentration that can inhibit the growth of P. fluorescens. Disc test reported that fraction 4 was the best fraction, with the dominant compounds that contained in the bulbs was phenolic compound based on FTIR test. So, the fraction of dayak onion bulbs (E. palmifolia (L) Merr) can be used as potential leads to discover new drugs.
Identification of Plankton on Fish Pond of Oreochromis niloticus Infected by Viral Nervous Necrosis Uun Yanuhar; Muhammad Musa; Dyah Tri Rahayu; Diana Arfiati
Research Journal of Life Science Vol 3, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (907.761 KB) | DOI: 10.21776/ub.rjls.2016.003.02.6

Abstract

The purpose of study is to determine the type of plankton found in cultivation pond of nile tilapia infected by VNN. The method is to identify the plankton in the water pond and testing the VNN that infects nile tilapia related to plankton eaten using Polymerase Chain Reaction (PCR). VNN identification results on the nile tilapia digestive tract stomach and intestines as well as plankton found in the stomach and intestines indicate VNN positively. Plankton found in the digestive tract of fish is division of Chlorophyta, Bacillariophyta, phylum Rotifera and Crustacea. It indicates that nile tilapia infected by VNN both on the gastrointestinal tract organs, stomatch or intestines and plankton were found with positive VNN digestive duct, while plankton found in water pond is VNN negative. The VNN infection depends on the nature of specifications VNN on nile tilapia and the plankton in the nile tilapia digestive duct.
Identification of Koi Herpesvirus on Koi Fish (Cyprinus carpio) with Immunocytochemistry Test Streptavidin Biotin Rika Wahyuningtyas; Uun Yanuhar
Research Journal of Life Science Vol 5, No 3 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (321.292 KB) | DOI: 10.21776/ub.rjls.2018.005.03.2

Abstract

Koi herpesvirus (KHV) has been identified in common carp (Cyprinus carpio) and varieties of C. carpio such as koi carp. Handling KHV outbreak on koi fish are always late and on case KHV infection, hence the purpose of this study is to find an alternative method in identifying a particular fish diseases KHV which more simple, practical and accurate.  20 samples are positively infected by KHV were derived from Blitar, with the characteristic of them are gill slime, erosion or ulcers on the skin, movement were not controlled, and skin blister. The result of our study showed that 100% positive reaction to the ICC in smear blood, which is marked golden chocolate color. The result of immunostaining streptavidin biotin obtained that koi fish have been infected by severe KHV showed golden chocolate color on the blood smear compared to positive control. While fish have been infected by undemanding KHV infection or still in the beginning stage, the blood smear showed chocolate bluish. It can be concluded that Immunocytochemistry Test Streptavidin Biotin can be used for early identifying of Koi Herpes Virus (KHV) due to simpler producer, more practical, and accurate result.
Co-Authors Abdul Rahem Faqih Ach. Khumaidi Adelya Desi Kurniawati Adhitya, Dewa Sukma Trinanda Agus Susilo Alfarisi, Muhammad Asnin Amasda, Naufal Rizky Aminnudin, Aminnudin Amira Baihani Amira Baihani Amira Masitha Anasiru, Arief Farhan Kurniawan Andi Kurniawan Andik Isdianto Anggraini, Rifka Rimbi Anisa Millah Taqiyyah Anjasmara, Aang Setyawan Anni Faridah Annisa Fitri Anggreini Apri Supii Asep Awaludin Prihanto Asus Maizar Suryanto H Asus Maizar Suryanto Hertika Awalia Oktaviana Bili Darnanto Susilo Binoj, Joseph Selvi BS, Sumitro Choirul Huda Choirul Huda Cucun Herlina Daimon Syukri Diana Arfiati Dwi Retna Kumalaningrum Dyah Kinasih Wuragil Dyah Kinasih Wuragil Dyah Kinasih Wuragil Dyah Tri Rahayu Dyah Wuragil E Ernawati, E Eddy Suprayitno Edi Susilo Endang Yuli Herawati Fadjar, Mohammad Fajar Shodiq Fathah, Aulia Lanudia Fauliza, Tifa feri Setiawan Fika Fitrianesia Fitri, Kharisma Nuria Fitrianesia, Fika Gatot Ciptadi Gede Angga Krishna Fariestha Gian Suryanatha Hartawan Gian Suryanatha Hartawan Gunanti Mahasri Hardoko Hardoko Herlina, Cucun Herly Evanuarini Herly Evanuarini Herly Evanuarini Heru Suryanto Immaria Fransira Indah Wahyuni Abida Irawanto, Yovan Endik Ismail, Mahrus Ita Fitria S Jati Batoro Jibril Maulana Komarudin Komarudin MA, Widodo Maftuch ., Maftuch Maftuch Maftuch Maheno Sri Widodo, Maheno Sri Maria Kristiani Marsono Marsono Martiningsih, Nur Fauzi-yah Martiningsih, Nur Fauziyah Masitha, Amira Mohammad Bisri Mohammad Fadjar Mohammad Musa Mufidah, Rhobithotus Muhamad Muhajir Muhammad Faiq Ash Shiddiq Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Sumsanto Muhammad Sumsanto Musa, Mohammad Nico Caesar Nico Rahman Caesar Nizery, Sefhanissa Puspa Retno Nur Fauziyah Martiningsih Nur Junirahma Nur Sakinah Junirahma Nusantara, Fajar Osman, Azlin Fazlina Pahlefi, Reval Pradana, Yanuar Rohmat Aji Purwoko, Agus Puspitasari, Intan Dwi Putri, Berlania Mahardika R Adharyan Islamy Ramadhanti, Nur Fa'izah Fitri Rifka Rimbi Anggraini Rika Wahyuningtyas Rika Wahyuningtyas Rika Wahyuningtyas, Rika Rizka Rahmana Putri Sanoesi, Ellana Sansi, Delfina Husniya Septi Anitasari, Septi Setyayunita, Tamaryska Shiddiq, Muhammad Faiq Ash Sias, Quota Alief Soelistyoadi, Rachmat Noer Soemarno Soemarno Sri Andayani Sri Andayani Sri Andayani Andayani Sri Rahmaningsih Sri Rahmaningsih Sukarni Sukarni Suprayitno Suprayitno Susilowati, Surya Rachman Taufik Budhi Pramono Taufik Budhi Pramono Teotonio Gomes, Guilherme Tri Djoko Lelono Ulhaq, Ulfieda Anwar Utari, Inpita Casuarina Eqisetia Wardani, Nezya Pramudya Wuragil, Dyah Kinasih Yenny Risjani Yovan Endik Irawanto Yusuf Arif Wahyudi Yusuf Arif Wahyudi Yusuf Wahyudi