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All Journal IAES International Journal of Artificial Intelligence (IJ-AI) HAYATI Journal of Biosciences PHARMACON Jurnal Bios Logos Animal Production : Indonesian Journal of Animal Production Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Makara Journal of Science Heca Journal of Applied Sciences Malacca Pharmaceutics Health & Medical Sciences Journal of Educational Management and Learning Infolitika Journal of Data Science Grimsa Journal of Science Engineering and Technology Borneo Journal of Pharmacy
TRINA EKAWATI TALLEI
Faculty of Mathematics and Natural Sciences, Sam Ratulangi University Manado
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Veterinary Other

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Comparative Study of DNA Barcoding and Mini-Barcoding based on COI Gene for Species Identification and Phylogeny of Formicidae Family (Insecta: Hymenoptera) Kolondam, Beivy Jonathan; Tallei, Trina Ekawati; Koneri, Roni; Mamahit, Juliet Merry Eva
HAYATI Journal of Biosciences Vol. 32 No. 6 (2025): November 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.6.1626-1642

Abstract

DNA barcoding and mini-barcoding are widely used to study insect biodiversity, including ants (Hymenoptera: Formicidae). This study evaluated the effectiveness of both approaches for species identification and phylogenetic inference in ants, with particular attention to the performance of universal primers during PCR amplification. Full-length DNA barcode (658 bp) and mini-barcode (127 bp) regions of the mitochondrial cytochrome c oxidase subunit I (COI) gene were retrieved from GenBank, aligned, and analysed with maximum-likelihood phylogenetics (1,000 bootstrap replicates) under the Tamura-Nei model. The universal DNA barcoding primers (Folmer's LCO1490 and HCO2198) showed generally good alignment with ant COI sequences. However, multiple nucleotide mismatches were observed in LCO1490, including one critical mismatch located within the GC clamp region. In contrast, the universal mini-barcoding primers (UniMinibarF1 and UniMinibarR1) showed critical mismatches at their 3′ ends, likely reducing PCR efficiency. Despite limitations associated with primer mismatches, both DNA barcoding and mini-barcoding are reliable for species identification. DNA barcoding and mini barcoding are both capable of distinguishing ants at the species level, regardless of the length of the sequences being compared. The same sequences have also been used for phylogenetic analysis, showing good ability to distinguish ant species based on phylogenetic tree reconstruction. The comparison of DNA barcoding and mini-barcoding in ants showed that longer sequences offered superior resolution for species identification and phylogenetic reconstruction. These findings underscore the need for primer optimisation in ant-specific applications and highlight the utility of both barcoding strategies for taxonomic and evolutionary studies.
The Potent Antimicrobial Spectrum of Patchouli: Systematic Review of Its Antifungal, Antibacterial, and Antiviral Properties Kemala, Pati; Idroes, Rinaldi; Khairan, Khairan; Ramli, Muliadi; Tallei, Trina Ekawati; Helwani, Zuchra; Rahman, Sunarti Abd
Malacca Pharmaceutics Vol. 2 No. 1 (2024): March 2024
Publisher : Heca Sentra Analitika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60084/mp.v2i1.156

Abstract

ntention towards natural essential oils from medicinal plants has increased rapidly over the past decade as these oils have antimicrobial and antioxidant properties against various chronic diseases. One essential oil source with antimicrobial properties is the essential oil from Pogostemon cablin (Blanco) Benth. This review aims to provide information on using patchouli oil as an antimicrobial against bacterial, fungal, and viral pathogens in the last five years. There were 37 articles found in the PUBMED database by June 15, 2023. After searching, 6 of them were duplicates. A total of 2 papers were inaccessible, 4 were not research articles, and five were excluded because they were irrelevant to the scope of this study. This review shows that research related to patchouli as an antimicrobial in the last five years involves Pogostemon cablin leaf samples as silver nanoparticle bioreductors. Patchouli oil is used in membrane, nanocomposite film, and starch hydrogel manufacturing. Patchouli oil is a prestigious antimicrobial agent because it can fight numerous pathogenic microbes from bacteria, fungi, and viruses.
Network Pharmacology Approach to Understanding the Antidiabetic Effects of Pineapple Peel Hexane Extract Pendong, Christa Hana Angle; Suoth, Elly Juliana; Fatimawali, Fatimawali; Tallei, Trina Ekawati
Malacca Pharmaceutics Vol. 2 No. 1 (2024): March 2024
Publisher : Heca Sentra Analitika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60084/mp.v2i1.162

Abstract

The increased interest in exploring alternative treatments for type 2 diabetes mellitus is accompanied by a rise in the prevalence of type 2 diabetes mellitus. Pineapple peel is one of the by-products of pineapple fruit and is known to possess potential for anti-diabetic activity. In this study, the n-hexane extract of pineapple peel was analyzed using network pharmacology methods to ascertain its potential in treating type 2 diabetes mellitus. The GC-MS analysis of the n-hexane extract of pineapple peel revealed the presence of 42 compounds, with 8 of them considered safe as they met the Lipinski Rule of Five criteria for drug-likeness and were classified as safe with toxicity levels in classes IV and V. The pineapple peel extract targeted 55 proteins related to type 2 diabetes mellitus (DMT2), potentially affecting DMT2 through the AGE-RAGE pathway in diabetes complications and insulin resistance. Network pharmacology analysis identified five genes targeted by pineapple peel, namely MAPK1, JAK2, MAPK8, PRKCD, and PPARA. Among these genes, MAPK1 exhibited a higher overall score than the others. Apart from its role in diabetes, MAPK1 is also implicated in cancer.
Exploring the Medicinal Potential of Blumea balsamifera: Insights from Molecular Docking and Molecular Dynamics Simulations Analyses Maulydia, Nur Balqis; Khairan, Khairan; Tallei, Trina Ekawati; Salaswati, Salaswati; Musdalifah, Annisa; Nabila, Fiki Farah; Idroes, Rinaldi
Malacca Pharmaceutics Vol. 2 No. 1 (2024): March 2024
Publisher : Heca Sentra Analitika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60084/mp.v2i1.168

Abstract

Blumea balsamifera from the Ie-Jue geothermal area in Aceh Province, Indonesia, has been reported to have a variety of secondary metabolites. However, there is limited information about the activity of these chemical metabolites from B. balsamifera. The aim of this study is to evaluate the therapeutic potential of these compounds using molecular docking and molecular dynamics simulations. Six selective compounds were thoroughly evaluated using molecular docking techniques for their inhibitory effects on both Coronavirus protease and human interleukin receptors. Additionally, druglikeness assessments based on the Lipinski rule of five were performed to evaluate these six ligands. Our results show that stigmasterol, a key component of B. balsamifera, has demonstrated low binding free energy values across four receptors. Furthermore, molecular dynamics simulations confirmed the stability of the top ligand-receptor complex, particularly stigmasterol-1IRA, based on five parameters, indicating its stability as an inhibitor. This research highlights the potential of stigmasterol as a therapeutic agent derived from medicinal plants of B. balsamifera and underscores the value of our molecular approach in identifying opportunities for pharmaceutical development.
Therapeutic Vaccines in Non-Small Cell Lung Cancer: Immunologic Mechanisms, Therapeutic Platforms, and Barriers to Efficacy Rumambi, Ekklesia Wulan Matilda; Tallei, Trina Ekawati; Turalaki, Grace Lendawati Amelia
Malacca Pharmaceutics Vol. 4 No. 1 (2026): March 2026
Publisher : Heca Sentra Analitika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60084/mp.v4i1.378

Abstract

Therapeutic vaccines for non-small cell lung cancer (NSCLC) aim to improve treatment outcomes for a disease with high global incidence, mortality, and recurrence risk despite receiving standard multimodal therapy. This field focuses on the use of cancer antigens as vaccine targets in the context of immunology, influenced by immunovigilance, immunoreduction, and the tumor microenvironment, which suppresses the immune system. Mechanistic requirements for effective vaccination include selecting cancer antigens that are highly and homogeneously expressed, functionally linked to oncogenic pathways, and efficiently presented via MHC molecules to coordinate T cell responses. Peptide-based, dendritic cell-based, nucleic acid-based, and microbial vector-based vaccine platforms demonstrate safety and induction of antigen-specific cellular immunity responses. However, survival remains moderate and inconsistent, particularly in advanced-stage patients. Future progress will depend on rigorous, mechanism-based design that integrates data-driven antigen and epitope selection with tailored platform and route selection to shape the desired immune response, while also facilitating personalized and optimized vaccination strategies.
The Evolving Landscape of the Colorectal Cancer Vaccines: From Biological Mechanisms to Translational Therapeutics Setiono, Sabrina Brigitta Valerie; Turalaki, Grace Lendawati Amelia; Tallei, Trina Ekawati
Heca Journal of Applied Sciences Vol. 4 No. 1 (2026): March 2026
Publisher : Heca Sentra Analitika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60084/hjas.v4i1.372

Abstract

Colorectal cancer (CRC) remains a major global health burden, and despite substantial advances in cancer immunotherapy, the clinical efficacy of therapeutic cancer vaccines in CRC has been limited. This review critically examines the biological, immunological, and translational factors that shape CRC vaccine development, with a particular focus on tumor immunopathology, antigen selection, vaccine platforms, and emerging combination strategies. We summarize current knowledge on CRC-associated tumor antigens, including selected tumor-associated antigens and neoantigen-based approaches, alongside major vaccine modalities evaluated in preclinical and early-phase clinical studies. Across the literature, vaccine-induced immunogenicity frequently exceeds demonstrated clinical benefit, highlighting a persistent translational gap. Synthesis of available evidence suggests that this gap is driven primarily by CRC-specific immune constraints, including immune exclusion, dominance of immunologically cold MSS/pMMR tumors, and tolerogenic pressures within metastatic niches, particularly the liver. We further discuss how rational combination strategies, especially those integrating cancer vaccines with immune checkpoint inhibitors (ICIs), may partially overcome these barriers. In addition, the review outlines the conceptual role of bioinformatics and immunoinformatics in supporting antigen prioritization, neoantigen discovery, and patient stratification in CRC vaccine research. Overall, this review emphasizes that future progress will depend on CRC-tailored antigen selection, mechanistically informed vaccine design, rational combination regimens, and rigorous clinical evaluation to define the realistic clinical role of therapeutic cancer vaccines in CRC.
Mechanistic Exploration of Moringa oleifera Leaf Bioactives in Prostate Cancer Using Network Pharmacology Tengke, Feniea Wulan Agaype; Tallei, Trina Ekawati; Turalaki, Grace Lendawati Amelia; Tendean, Lydia Estelina Naomi; Kaseke, Martha Marie; Niode, Nurdjannah Jane; Fatimawali, Fatimawali
Grimsa Journal of Science Engineering and Technology Vol. 4 No. 1 (2026): April 2026
Publisher : Graha Primera Saintifika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61975/gjset.v4i1.105

Abstract

Prostate cancer remains one of the most prevalent malignancies in men worldwide, while current therapeutic strategies are often associated with adverse effects and the emergence of drug resistance, highlighting the need for safer and more effective alternatives. This study aimed to elucidate the multitarget molecular mechanisms of bioactive compounds derived from Moringa oleifera leaves against prostate cancer using an integrated in silico network pharmacology approach. A total of 137 bioactive compounds were collected from LC–MS/MS profiling data and phytochemical databases, of which 20 compounds with high predicted biological activity were prioritized based on Way2Drug PASS analysis. Potential protein targets were predicted and intersected with prostate cancer–associated genes to identify shared targets. Protein–protein interaction network analysis revealed 536 overlapping targets, with ten hub proteins, ESR1, AKT1, SRC, EGFR, TP53, HSP90AA1, PIK3CA, HSP90AB1, PIK3R1, and MAPK1, identified as central nodes. Pathway enrichment analysis demonstrated that these targets were predominantly involved in cancer-related signaling pathways, including pathways associated with EGFR tyrosine kinase inhibitor resistance. Pharmacokinetic and toxicity assessments indicated that several compounds, such as isorhamnetin, kaempferol, tocopherol, and afzelin, exhibited favorable drug-likeness properties and low predicted toxicity. Overall, these findings suggest that bioactive compounds from Moringa oleifera leaves exert anticancer effects through a multitarget and multi pathway mode of action rather than single-protein modulation. This study provides systematic insight into the molecular mechanisms underlying the anti–prostate cancer potential of Moringa oleifera leaves and supports their relevance as candidates for further experimental validation and drug development.
Co-Authors Abas, Abdul Hawil Adikila, Gregorius Giani Angelina Stevany Regina Masengi Antasionasti, Irma Any Aryani Arifin, Mulyani Asep Rusyana Azzania Fibriani Balansa, Endrile Golmen Barasarathi , Jayanthi BEIVY JONATHAN KOLONDAM Celik, Ismail Daniel Febrian Sengkey Dantje Tarore Diah - Kusumawaty Diah Puspitasari Dian Handayani Diana Setya Ningsih, Diana Didik Priyandoko Dolongtelide, Jeclin Inebel Dzikrina, Hanina El-Shazly, Mohamed Elly Suoth Emran, Talha Bin Erwin Wantasen Estevam, Ethiene Castellucci Faisal, Farassa Rani Fatimawali . Florencia N. Sompie Ghazi Mauer Idroes Halimatushadyah, Ernie Hariyanto, Yuanita Amalia Herny E.I. Simbala Hizir Sofyan Idroes, Ghifari Maulana Illah Sailah Irvanizam, Irvanizam Jein Rinny Leke, Jein Rinny Kalalo, Marko Jeremia Kemala, Pati Kepel, Billy Johnson Khairan Khairan Laksono Trisnantoro Lala, Andi Lydia E. N. Tendean, Lydia E. N. Mamahit, Juliet Merry Eva Martha Marie Kaseke Masengi, Kyoko Itsuko Etsuko Gabriela Maulana, Aga Maulydia, Nur Balqis Mirda, Erisna Moh. Yani Mohd Fauzi, Fazlin Muliadi Ramli Musdalifah, Annisa Nabila, Fiki Farah Niode, Nurdjannah Jane Nurul Faridah, Nurul Patwekar, Mohsina Paulina yamlean Pendong, Christa Hana Angle Purukan, Christy Purwanto, Diana Shintawati Rahman, Sunarti Abd Ratte, Titah Amelia Rinaldi Idroes Rizkia, Tatsa Roni Koneri Rumambi, Ekklesia Wulan Matilda Runtunuwu, Stefanus Vicky Bernhard Elisa Salaki, Christina Leta Salaswati, Salaswati Sambul, Alwin Melkie Sari, Nadia Warda Sekar Sasmita, Novi Reandy Setiono, Sabrina Brigitta Valerie Siampa, Jainer Pasca Sri Sudewi, Sri Takawaian, Agrita Feisilia Tamala, Yulianida Tania, Adinda Dwi Tendean, Lydia Estelina Naomi Tengke, Feniea Wulan Agaype Teuku Rizky Noviandy Tumilaar, Sefren Geiner Turalaki, Grace Lendawati Amelia Unsratdianto Sompie, Sherwin Reinaldo Utami, Wulandari Putri Wawo, Arsianita Ester Wijaya, Puspita Wungouw, Herlina Ineke Surjane Zuchra Helwani, Zuchra Zulkarnain Jalil