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37% Phosphoric Acid Induced Stronger Matrix Metalloproteinase-8 Expression of the Dental Pulp than 19% Ethylene Diamine Tetraacetic Acid Fatimatuzzahro, Nadie; Haniastuti, Tetiana; Handajani, Juni
Journal of Tropical Life Science Vol 4, No 3 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

37% Phosphoric Acid Induced Stronger Matrix Metalloproteinase-8 Expression of the Dental Pulp than 19% Ethylene Diamine Tetraacetic Acid Nadie Fatimatuzzahro; Tetiana Haniastuti; Juni Handajani
Journal of Tropical Life Science Vol. 4 No. 3 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Etching agents such as ethylene diamine tetraacetic acid (EDTA) and phosphoric acid which are widely used in adhesive restoration system aimed to increase for retention of restorative materials, may act a chemical irritant that induce inflammation of dental pulp. Inflammation is a body response against irritant and infectious agents. Matrix metalloproteinase-8, the major collagenolytic enzyme, degrades collagen type 1. This enzyme is expressed in low level in normal condition, however, the expression will increase during inflammation. The purpose of the present research was to study the effect of 19% EDTA and 37% phosphoric acid application as an etching agents on the MMP-8 expression of dental pulp. Forty-five male Sprague Dawley rats were divided into 3 groups. Cavity preparation was made on the occlusal surface of maxillary first molar using a round diamond bur. 19% EDTA, 37% phosphoric acid, and distilled water were applied on the surface of the cavity of the teeth in group I, II, and III subsequently. The cavity then filed by glass ionomer cements. The rats were sacrified at 1, 3, 5, 7, and 14 days after the treatment (n=3 for each day). The specimens were then processed histologically. Immunohistochemical (IHC) analysis was performed using rabbit anti rat MMP-8 polyclonal antibody to examine MMP-8 expression and HE (Hematoxylen Eosin) staining to observe the number of macrophages. The results showed 37% phos­phoric acid application induced stronger expression of MMP-8 and higher number of macrophages than 19% EDTA. The strongest expression of MMP-8 seems on 5 days after the treatment where the highest number of macrophages were also found.
Co-Authors ABDUL MUNIF Achmad Gunadi Adinda Febriyanti Aditya Satriatama, Dandy Agusta, Akhmad Riandy Amandia D.P.Shita Amandia Dewi Permana Shita Anggia Rahmania, Dwi Anita Faizah Aulia Vanadia Baladraf, Thabed Tholib Bambang Sumarsono, Raden Bertung Suryadharma, Bertung Depi Praharani Desi Eri Kusumaningrum Dewi Pratiwi Dina Amalia Diva Pujiaryanti, Anindya Dwi K.Apriyono Dwi Kartika Apriyono Erawati Wulandari Faiqatin Cahya Ramadhani Farah Rachmah Aulia Wardani Farchi, Ghafran Nailul Fathul Jannah, Fathul Favinas Octa Nuri Tsalats Fiftiani Syarah Firnanda, Rara Ayu Hafiedz Maulana Heri Kristianto Hidayat, Ari I Dewa Ayu Ratna Dewanti I Dewa Ayu Susilawati Ida Bagus Suryaningrat Indiyanti, Nurul Fitria Indonesia Koes Wijayaningrat Irvan, Mohamad Juni Handajani Kusumawardhani, Amelia Laila Efendi, Anggi Laksmi Indreswari Maisyaroh Miftahul Choiron Mirsodi, Muhammad Novi Khila Firani Nur Hidayati Nurul Iffah, Astrid Ganadya Nuzulul Hikmah Peni Pujiastuti Permana Shita, Amandia Dewi Pintan Qorina Destianingrum Pranata, Dadin Gilang Pratiwi , Dewi Pratiwi, Yossy Retna Putri Tio, Aurelia Jovita Putri, Syifa Lestiya Rahardyan Parnaadji Raissa Salma Hardiani Renda Shania Alicia Rendra C.Prasetya Rendra Chriestedy Prasetya Rokhani Rokhani Romdhlo, Komsiatun Rusdianto, Andrew Setiawan Sampurna, Hadi Shinta Puri Sitanggang, Della Supriyadi Supriyadi SYARIFUDDIN SYARIFUDDIN Syawalna, Alifian Widyputra Tantin Ermawati Teguh Triwiyanto Umaimah, Ulfa Umniyya, Anisatul Viana, Eka Dasra Widiyanti, Ainurrohma Winda Amilia