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All Journal Journal of Food and Pharmaceutical Science Jurnal Tumbuhan Obat Indonesia Jurnal Penelitian Pascapanen Pertanian Majalah Obat Tradisional Journal of Tropical Biodiversity and Biotechnology INDONESIAN JOURNAL OF PHARMACY The Indonesian Biomedical Journal Indonesian Journal of Chemical Science Jurnal Farmasi Sains dan Komunitas Majalah Farmaseutik JPSCR : Journal of Pharmaceutical Science and Clinical Research Jurnal Ilmu Kefarmasian Indonesia Journal of Fundamental and Applied Pharmaceutical Science Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Journal of Food and Pharmaceutical Sciences Jurnal Tumbuhan Obat Indonesia Jurnal Kefarmasian Indonesia

Nanang Fakhrudin, Nanang

Department Of Pharmaceutical Biology, Faculty Of Pharmacy, Universitas Gadjah Mada, Jl. Sekip Utara, Sleman, Yogyakarta, Indonesia, 55281.

Author-ID : 514358

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health

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Potensi Antioksidan Biji dan Daun Kacang Hijau (Vigna radiate L.) dan Studi Korelasinya Dengan Kadar Flavonoid Total Nanang Fakhrudin; Nimas Ayu Kurniailla; Kahfi Nur Fatimah
Jurnal Penelitian Pascapanen Pertanian Vol 17, No 1 (2020): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v17n1.2020.48-58

Biji dan daun kacang hijau (KH) mengandung senyawa flavonoid sebagai antioksidan yang bermanfaat bagi kesehatan. KH dapat diekstrasi dengan air dan etanol menghasilkan ekstrak kaya flavonoid untuk bahan baku produk suplemen kesehatan dan obat tradisional. Penelitian bertujuan membandingkan potensi antioksidan dari biji dan daun KH, menganalisis kandungan flavonoid dan korelasinya dengan potensi antioksidan. Biji dan daun KH diekstraksi dengan air dan etanol masing-masing menggunakan metode dekokta dan maserasi. Potensi antioksidan ekstrak dievaluasi dengan metode FRAP menggunakan trolox sebagai pembanding, dan nilainya dinyatakan sebagai ekuivalensi terhadap trolox per gram ekstrak. Kadar flavonoid total ditetapkan secara spektrofotometri dengan rutin sebagai pembanding. Analisis kandungan flavonoid ekstrak dilakukan dengan kromatografi lapis tipis. Ekstraksi air biji KH memiliki potensi antioksidan 69,65±10,89 µmol TE/g, lebih tinggi dari daunnya 40,52±3,33µmol TE/g. Pada ekstraksi dengan etanol, potensi antioksidan biji KH lebih besar daripada daunnya (131,50±12,77µmol TE/g dibanding 9,37±0,42 µmol TE/g). Ekstrak air biji dan daun KH masing-masing memiliki kadar flavonoid total 2,98±0,03 dan 2,64±0,29 %, sedangkan ekstrak etanolnya masing- masing sebesar 4,71±0,72 dan 1,06±0,16 %. Kadar flavonoid ekstrak sebanding dengan potensi antioksidannya. Vitexin dan rutin merupakan senyawa flavonoid utama dalam biji dan daun KH. Daun KH juga memiliki potensi antioksidan yang cukup kuat dan kaya akan senyawa flavonoid. Antioxidant Potency of Mung Bean (Vigna radiate L) Seed and Leaf and Its Correlation with The Total Flavonoid Content.Mug Bean (MB) seeds dan leaves contain flavonoids with promising antioxidant activity. MB can be extracted with water and ethanol to yield flavonoid-enriched extract for health supplement and traditional medicine products raw material. This study aimed to compare the antioxidant potency of MB seed and leaf, and to analyze the flavonoid content and its correlation with antioxidant potency. MB seed and leaf were extracted with water and ethanol using decocta and maceration, respectively. The antioxidant potency of the extracts was evaluated by the FRAP method using Trolox as a reference control. The antioxidant potency was expressed as Trolox equivalent per gram of extract. The total flavonoid contents were determined spectrophotometrically with Rutin as a reference compound. Analysis of flavonoid component in the extracts was done by thin layer chromatography. The water extracts of MB seeds and leaves demonstrated antioxidant potency of 69.65±10.89 and 40.52±3.33 µm TE/g, whereas the ethanol extracts were 131.50±12.77 and 9.37±0.42 µmol TE/g, respectively. The water extracts of MB seeds and leaves have total flavonoid content of 2.98±0.03 and 2.64±0.29 %, whereas the ethanol extracts were 4.71±0.72 and 1.06±0.16 %, respectively. We found that the total flavonoid content is proportional with the antioxidant potency. Vitexin and Rutin are the main flavonoids in MB. MB leaves also showed a powerful antioxidant potency and are rich of flavonoids.

KAJIAN ETNOBOTANI, FITOKIMIA, FARMAKOLOGI DAN TOKSIKOLOGI SUKUN (Artocarpus altilis (Park.) Fosberg) Gharsina Ghaisani Yumni; Sitarina Widyarini; Nanang Fakhrudin
Jurnal Tumbuhan Obat Indonesia Vol 14 No 1 (2021): Jurnal Tumbuhan Obat Indonesia
Publisher : Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jtoi.v14i1.3944

ABSTRACT Indonesia is a country with large plant biodiversity with medicinal properties, such as “sukun” (Artocarpus altilis) or known as “breadfruit”. Breadfruit is a woody evergreen plant that has been used traditionally for various purposes, including medication. The fruit is rich in carbohydrates and fibers as a food source. The leaf and cortex are the most widely used for treating various diseases and other health benefits. This article aimed to present a comprehensive review on the potency of breadfruit from the perspective of ethnobotany, phytochemistry, pharmacology, and toxicology. The data in this narrative review was obtained from the scientific journals in the databases of Google Scholar, PubMed, Scopus, and ScienceDirect. Other credible sources, such as textbooks, student thesis, and patents were also used to support the main data. Based on the literature study, breadfruit has been used empirically in Indonesia as a medicinal herb. The scientific data of breadfruit showed antiinflammatory, antiplatelet, antioxidant, antiatherosclerosis, antihyperlipidemic, antimalaria, antidiabetic, cardioprotective, and anticancer activities. Breadfruit contains terpenoids, flavonoids, alkaloids, and phenolics as bioactive compounds. However, the unique compounds are geranylated and prenylated flavonoids such as cycloartenol, artonin V, and cyclomulberin. These compounds are distributed in the leaf, cortex, wood, and fruit. Limited data is available regarding the toxicology profile of breadfruit. Breadfruit leaves ethanol extract did not show any significant toxic effects in the animal experiments. However, the toxicity of the water extract is unclear, and thus, needs to be investigated to ensure its safety. Keywords: Artocarpus communis, bioactivity, chemical constituents, ethnopharmacology ABSTRAK Indonesia dikenal sebagai negara yang kaya akan keragaman tumbuhan berpotensi obat, diantaranya sukun (Artocarpus altilis). Sukun merupakan tanaman berkayu yang secara tradisional dimanfaatkan untuk berbagai keperluan termasuk pengobatan. Buah sukun mengandung karbohidrat dan serat sebagai sumber pangan. Daun dan batang sukun merupakan bagian yang paling banyak dimanfaatkan dalam pengobatan dan kesehatan. Reviu artikel ini bertujuan untuk mengkaji secara komprehensif potensi sukun dari sudut pandang etnobotani, fitokimia, farmakologi, dan toksikologi. Artikel narrative review ini ditulis berdasarkan data yang diperoleh dari kajian literatur hasil penelitian yang ada di basis data Google Scholar, PubMed, Scopus, dan ScienceDirect. Beberapa sumber pustaka lain seperti buku, naskah tugas akhir dan paten juga digunakan untuk memperkaya penulisan. Hasil kajian literatur sukun menunjukkan bahwa tanaman ini memiliki riwayat empiris digunakan sebagai obat tradisional di Indonesia. Hasil penelitian ilmiah menunjukkan sukun memiliki aktivitas antiinflamasi, antiplatelet, antioksidan, antiatherosklerosis, antihiperlipi-demia, antimalaria, antidiabetes, kardioprotektif, dan antikanker. Sukun mengandung senyawa terpenoid, flavonoid, alkaloid, dan senyawa fenolik. Senyawa khas dari tumbuhan genus Artocarpus ini adalah flavonoid dengan gugus geranil atau prenil, misalnya sikloartenol, artonin V, dan siklomulberin. Senyawa tersebut tersebar dalam daun, kulit kayu, batang, dan buah. Data terkait profil toksikologi sukun masih terbatas. Ekstrak etanol daun sukun tidak menunjukkan efek toksik pada hewan uji. Namun, ekstrak airnya belum memiliki profil toksikologi yang jelas sehingga perlu dilakukan pengujian untuk memastikan keamanannya. Kata kunci: Artocarpus communis, bioaktivitas, kandungan kimia, etnofarmakologi

STUDI AKTIVITAS ANTIPLATELET DAN ANTITROMBOSIS EKSTRAK AIR DAUN SUKUN (Artocarpus altilis (Park.) Fosberg) Indah Hastuti; Arief Nurrochmad; Ika Puspitasari; Nanang Fakhrudin
Jurnal Tumbuhan Obat Indonesia Vol 14 No 1 (2021): Jurnal Tumbuhan Obat Indonesia
Publisher : Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jtoi.v14i1.4227

ABSTRACT The mature breadfruit leaves (Artocarpus altilis) infusion has been traditionally used by Indonesian folks for curing heart diseases and stroke. The key mechanisms underlying these diseases are platelet aggregation and thrombosis. There is no evidence about the efficacy of the water extract of A. altilis leaves (EADS) against platelet aggregation and thrombosis, in order to provide scientific evidence regarding its use by the community. This study aimed to investigate the antiplatelet and antithrombotic activities of EADS. Ticagrelor, an antiplatelet drug agonist of P2Y12 receptor was used as a positive control. The antiplatelet activity of EADS was assessed in vitro by Light Transmittance Aggregometry (LTA) method using human platelet induced by Adenosine Diphosphate (ADP); whereas the antithrombotic activity was evaluated in vivo using Acute Pulmonary Thromboembolism (APT) method in male adult Swiss mice induced by the mixture of epinephrine (0.7 mg/kg bw) and collagen (11 mg/kg bw). The number and the onset of dead and paralysis mice were observed; and the number of thrombus was calculated under the microscope. We found that EADS demonstrated a weak antiplatelet activity (IC50>1000 µg/mL). Based on the number and the onset of dead and/or paralysis, as well as the number of thrombus, we found that EADS failed to exhibit antithrombotic activity at the doses of 200, 300, and 400 mg/kg bw. TLC analysis showed that EADS did not contain 2-geranyl-2,3,4,4’-tetrahydroxydihydrochalcone (GTDC), the antiplatelet compound in the ethanolic extract of A. altilis leaves (EEDS) in our previous research. Keywords: Artocarpus altilis, platelet aggregation, antithrombotic, Light Transmittance Aggregometry, Acute Pulmonary Thromboembolism ABSTRAK Rebusan daun sukun yang sudah tua (Artocarpus altilis) secara tradisional digunakan oleh masyarakat Indonesia untuk mengobati penyakit jantung dan stroke. Agregasi platelet dan trombosis merupakan faktor penting pada patofisiologi kedua penyakit tersebut. Penelitian aktivitas antiplatelet dan antitrombosis dari Ekstrak Air Daun Sukun (EADS) belum pernah dilaporkan. Penelitian ini bertujuan untuk menguji aktivitas antiplatelet dan antitrombosis dari EADS guna memberikan bukti ilmiah terkait pemanfaatannya oleh masyarakat. Ticagrelor, obat antiplatelet yang merupakan agonis dari reseptor P2Y12 digunakan sebagai kontrol positif. Uji aktivitas antiplatelet dilakukan secara in vitro menggunakan metode Light Transmittance Aggregometry (LTA) dengan platelet yang diambil dari darah manusia dan digunakan induktor agregasi platelet berupa Adenosin Difosfat (ADP, 10µM). Parameter yang diamati adalah persen penghambatan agregasi platelet. Uji aktivitas antitrombosis dilakukan secara in vivo menggunakan metode Acute Pulmonary Thromboembolism (APT) pada mencit jantan dewasa galur Swiss dengan induktor trombosis berupa campuran epinefrin (0,7 mg/kgBB) dan kolagen (11 mg/kgBB). Parameter yang diamati adalah jumlah dan onset kematian, paralisis, serta jumlah trombus berdasarkan analisis histopatologi. Hasil uji menunjukkan bahwa EADS memiliki aktivitas antiplatelet yang lemah (IC50>1000 µg/mL). EADS tidak memiliki aktivitas antitrombosis yang terlihat dari ketidakmampuan dalam melindungi mencit dari paralisis dan/atau kematian serta tidak adanya penurunan jumlah trombus yang bermakna pada mencit yang diberi perlakuan ekstrak dibandingkan kelompok kontrol pelarut. Analisis KLT menunjukkan bahwa EADS tidak mengandung senyawa aktif antiplatelet 2-geranyl-2,3,4,4’-tetrahydroxydihydrochalcone (GTDC) yang ada dalam ekstrak etanol daun sukun (EEDS) pada penelitian sebelumnya. Kata Kunci: Artocarpus altilis, agregasi platelet, antitrombosis, Light Transmittance Aggregometry, Acute Pulmonary Thromboembolism

Profil Proliferasi Sel Limfosit Benalu Batu (Begonia medicinalis) Asal Kabupaten Morowali UTARA Provinsi Sulawesi Tengah Akhmad Khumaidi; Agustinus Widodo; Arsa Wahyu Nugrahani; Ediati Sasmito; Nanang Fakhrudin
JURNAL ILMU KEFARMASIAN INDONESIA Vol 18 No 1 (2020): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

The plant of Benalu Batu (Begonia medicinalis) from the North Morowali regency has been used empirically and is known to have anticancer activity. The study aims to determine the profile of the activity of lymphocytes cell proliferation of extracts and fractions of Benalu Batu in vitro as well to measure the correlation of total flavonoid content against the stimulating index of lymphocytes cell proliferation. The extraction of simplicia by using the maceration method with methanol solvent. The liquid-liquid partition method is used in the fractionation of methanol extracts with hexane, ethyl acetate, and water solvents, sequentially. Test of lymphocytes cell proliferation using MTT reduction (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method with concentration series 10, 20, 50, and 100 μg/mL. The total of flavonoids content of extracts and fractions were determined by using colorimetric method. The stimulation index (IS) is used in measuring the activity of lymphocytes cell proliferation. The test results showed that the methanol extracts, hexane fraction, ethyl acetate, and water may increase the proliferation of lymphocytes in the entire test concentration. The concentration of test influences on increasing the stimulation of the proliferation of lymphocytes cells. A test concentration of 100 μg/mL has the highest stimulation index on each test sample and its stimulant index value of hexane fraction, ethyl acetate fraction, methanol fraction and water fraction were 10.12, 6.56, 4.82, and 4.17, respectively. Total flavonoids content and stimulation indices have very low correlation (r = 0,082) and concentrations of 10 μg/mL have the highest correlation coefficient (r2 = 0.18). The results showed that the hexane and ethyl acetate fractions can be developed as immunostimulant materials for the anticancer supportive therapeutic agents despite having a very low correlation to the flavonoid levels.

Pembuatan Sediaan Tabir Surya Ekstrak Etanol Buah Mahkota Dewa (Phaleria macrocarpa), Aktivitas Inhibisi Fotodegradasi Tirosin dan Kandungan Fenolik Totalnya Tatang Irianti; T. N. Syaifullah Sulaiman; Nanang Fakhrudin; Siluh Astuti; Nita Testikawati; Sofa Farida; Sari Rosiati Nur Khasanah
Majalah Farmaseutik Vol 15, No 2 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Senyawa fenolik mempunyai potensi sebagai fotoprotektor, sehingga mampu melindungi kulit dari kerusakan oksidatif terutama menyerap Ultra Violet dari pancaran sinar matahari. Ekstrak etanolik buah masak mahkota dewa [Phaleria macrocarpa (Scheff) Boerl.] mengandung senyawa fenolik dan memiliki peranan sebagai fotoprotektor. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak etanolik buah mahkota dewa sebagai fotoprotektor dalam sediaan tabir surya dan oksibenson sebagai pembanding. Aktivitas fotoprotektor ditentukan dari tingkat fotodegradasi tirosin dan kadar fenolik totalnya, kemudian penelitian ini dilakukan dengan 8 formula; formula 1 sebagai kontrol negatif (basis); formula 2 sebagai kontrol positif (basis dan oksibenson); formula 3, formula 4, dan formula 5 mengandung ekstrak etanolik buah mahkota dewa dengan berbagai konsentrasi; formula 6, formula 7, dan formula 8 mengandung oksibenson dan ekstrak etanolik buah mahkota dewa dengan berbagai konsentrasi. Penentuan kemampuan fotoproteksi berdasarkan persen transmisi eritema dan persen transmisi pigmentasi. Uji inhibisi fotodegradasi dilakukan dengan menggunakan enam kelompok komposisi tirosin dan Rhetoflam. Uji penentuan kadar fenolik total dilakukan menggunakan metode Follin-Ciocalteu. Hasil pengukuran diintrapolasikan ke dalam kurva baku asam galat. Ekstrak etanolik buah mahkota dewa dalam sediaan tabir surya memberikan nilai Te sebesar 17,86-37,01 % dan nilai Tp sebesar 12,07-22,64 %. Efektivitas fotoproteksi ekstrak etanolik buah mahkota dewa lebih kecil dibandingkan oksibenson yang memiliki nilai Te sebesar 1,15.10-5 % dan Tp sebesar 0,67 %. Kombinasi ekstrak etanolik buah mahkota dewa dan oksibenson memberikan nilai Te sebesar 0,17-0,52 % dan nilai Tp sebesar 2,10-4,48 %. Sedangkan kandungan fenolik total ekstrak buah mahkota dewa adalah sebesar 8,29 ± 0,27 % dengan aktivitas penghambatan fotodegradasi tirosin 1,58 kali lebih besar dibandingkan dengan vitamin C pada kadar yang sama, yaitu 0,15%. Hal ini menunjukkan bahwa ekstrak etanolik buah mahkota dewa dapat dimanfaatkan sebagai bahan 4aktif fotoprotektor dalam sediaan kosmetik.

Pembuatan Sediaan Tabir Surya Ekstrak Etanol Rimpang Temu Kunci (Boesenbergia pandurata (Roxb.) Schlecht), Aktivitas Inhibisi Fotodegradasi Tirosin dan Kandungan Fenolik Totalnya Tatang Irianti; Teuku Nanda Saifullah Sulaiman; Nanang Fakhrudin; Siluh Astuti; Nita Testikawati; Soda Farida; Jovanita Fara Addina
Majalah Farmaseutik Vol 16, No 2 (2020)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Indonesia merupakan negara tropis dengan intensitas sinar matahari yang tinggi sehingga hal ini dapat memberikan dampak negatif terhadap kulit manusia. Untuk mencegah kerusakan akibat dari cahaya matahari diperlukan suatu bahan yang bersifat fotoprotektor dengan mekanisme kerja menyerap atau menghalangi cahaya matahari masuk ke kulit. Flavonoid ekstrak etanol rimpang temu kunci (Boesenbergia pandurata (Roxb.) Schlecht) diketahui memiliki aktivitas sebagai fotoprotektor. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol rimpang temu kunci dalam sediaan tabir surya yang mengandung bahan aktif oksibenson sebagai fotoprotektor; mengetahui aktivitas fotodegradasi tirosin serta kadar fenolik total ekstrak etanol rimpang temu kunci. Pada penelitian ini dilakukan dengan menguji delapan formula. Penentuan kemampuan fotoproteksi berdasarkan nilai transmisi eritema dan transmisi pigmentasi. Uji inhibisi fotodegradasi dilakukan dengan menggunakan enam kelompok komposisi tirosin dan Rhetoflam. Uji penentuan kadar fenolik total dilakukan menggunakan metode Follin-Ciocalteu. Hasil pengukuran diintrapolasikan ke dalam kurva baku asam galat. Penambahan ekstrak etanol rimpang temu kunci mampu menurunkan transmisi eritema 0,72-0,76 kali dan transmisi pigmentasi 0,57-0,61 kali dari sediaan tabir surya. Pemberian ekstrak etanol rimpang temu kunci 6% pada sediaan tabirsurya mengandung oksibenson 6% meningkatkan efektivitas tabir surya dengan menurunkan transmisi eritema 1,00 kali dan transmisi pigmentasi 1,02 kali lebih baik dari sediaan tabir surya mengandung bahan aktif oksibenson saja. Sedangkan kandungan fenolik total ekstrak etanol ripang temu kunci adalah sebesar 7,11 ± 0,15 % EAG dengan aktivitas penghambatan fotodegradasi tirosin 2,06 kali lebih besar dibandingkan dengan vitamin C pada kadar yang sama, yaitu 0,15%. Hal ini menunjukkan bahwa ekstrak etanolik rimpang temu kunci dapat dimanfaatkan sebagai bahan aktif fotoprotektor dalam sediaan kosmetik.

Mitragyna speciosa: Opioid Addiction Treatment and Risk of Use Azka Muhammad Rusydan; Endang Lukitaningsih; Nanang Fakhrudin
JPSCR: Journal of Pharmaceutical Science and Clinical Research Vol 7, No 2 (2022)
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jpscr.v7i2.50942

Kratom (Mitragyna speciosa (Korth.) Havil.) is a plant that originated from the rainforest in Southeast Asia, mainly grows in Thailand, Malaysia, and Indonesia. Kratom has been used traditionally as an herbal remedy for the treatment of various illnesses. Kratom gained notoriety due to its potential as an analgesic, opiate withdrawal treatment, anxiolytic, antidepressant, and antidiabetic with an unclear risk of addiction and toxicity fueled by a false sense of security due to its identity as a member of the coffee family. This article is a narrative review on kratom to highlight its pharmacological and toxicological properties, and the analytical method of Kratom, especially its potential as an opioid withdrawal therapy and its risk of abuse.

The Effect of Ursolic Acid from Plantago Lanceolata Leaves on Leukocytes Migration and Chemokines Level Nanang Fakhrudin; Yuvianti Dwi Franyoto; Eny Dwi Astuti; Arief Nurrochmad; Subagus Wahyuono
Indonesian Journal of Pharmacy Vol 30 No 4, 2019
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Initially considered as a normal body response to injury, inflammation is currently known as a major event contributing to the development of many human disorders. Many drugs and bioactive molecules have been discovered from medicinal plants and the number is still growing by time. Among those medicinal plants used in folk medicines, Plantago lanceolata is used to cure inflammatory-related diseases. In our previous study, we showed that the n-hexane insoluble fraction of P. lanceolata leaves (HIF) demonstrated a potent anti-inflammatory activity by inhibiting leukocytes migration in mice. This study aimed to identify the anti-inflammatory compound from the HIF and to investigate the effect on the chemokines level. P. lanceolata leaves were initially macerated with dichloromethane. The dried extract was partitioned using n-hexane to obtain n-hexane soluble fraction (HSF) and n-hexane insoluble fraction (HIF). Both fractions were evaluated for their anti-inflammatory activities in thioglycollate-induced leukocyte migration. The active fraction (HIF) was subjected to preparative thin-layer chromatography (TLC) to isolate the major compound. The structure of the compound was identified based on NMR, IR, and Mass spectra. Moreover, we investigated the effect of the compound on the level of chemokines responsible for leukocytes migration. The active compound was identified as ursolic acid, based on its spectral data. Ursolic acid at the dose of 30, 60, and 120mg/kg BW inhibited leukocyte migration and reduced chemokines level (IL-8 and MCP-1).

Identification Bioactive Compound of Ethanol-Water Fraction of Coleus atropurpureus for Anti-rheumatic Rheumatism in CFA-induced Rats Ipang Djunarko; Nanang Fakhrudin; Arief Nurrochmad; Subagus Wahyuono
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 19, No 2 (2022)
Publisher : Sanata Dharma University

Nonsteroidal anti-inflammatory drugs are used for pain and to slow the progression of rheumatoid arthritis. Accordingly, the discovery of rheumatoid arthritis active compounds from the ethanol-water fraction Coleus atropurpureus (EWC) was conducted to characterize the isolated compounds as well as the anti-rheumatic effects of the EWC induced Complete Freund's Adjuvant (CFA). We conducted in vivo study in rats which were randomly divided into 5 groups. Group 1 was only given CFA as a negative control. Group 2 as positive control was orally exposed to diclofenac potassium (9 mg/BW). Three groups were given different EWCs orally as follows: 11.25 mg/BW, 22.5 mg/BW, and 45 mg/BW, respectively. Rheumatism rates were then compared with positive controls using a visual arthritic scoring system. The compounds identified by isolation of the EWC of Coleus atropurpureus predicted forskolin. The ethanol-water fraction Coleus atropurpureus did not act as an anti-rheumatic arthritis agent in CFA-induced rats.

Phytochemical and Pharmacological Activities of Curcuma purpurascens Blume, A Review Oktariani Pramiastuti; Subagus Wahyuono; Nanang Fakhrudin; Puji Astuti
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 1 (2023): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.75891

Curcuma sp. is generally used for medicine, starch sources, preservatives, dyes and cosmetics. The use of Curcuma spp. for medical has increased because there have been many studies related to its active ingredients, such as flavonoids, essential oils, tannins, quinones, and terpenoids, as well as pharmacological activities, including wound healing, antioxidants, antifungal, anticancer, gastroprotective, and hepatoprotective. Curcuma purpurascens Blume is a species of Curcuma from family Zingiberaceae and used for traditional medicine. This article focuses on reviewing the literatures on C. purpurascens and discussing its morphology, phytochemical content, and pharmacological aspects. The method used to review this article was by exploring several databases such as Scopus, Pub Med, and Google Scholar to identify and download original articles and research journals related to the morphology, phytochemical content, and biological activity of Curcuma purpurascens Blume. The result of this review will later provide information about the uses and presence of Curcuma purpurascens Blume which is still rarely studied so further study related to its pharmacological activity tests and active compound as natural medicines can be explored.

Co-Authors Abdul Rohman Agustinus Widodo Akhmad Khumaidi Anak Agung Gede Sugianthara Andayana Puspitasari Gani Andayana Puspitasari Gani Argandita Meiftasari Arief Nurrochmad Arief Nurrochmad Arief Nurrochmad Arief Nurrochmad Arifah, Mitsalina Fildzah Ayu Ina Solichah Chelvin Ari Kusnanto Dirman, Arifin Djoko Santosa Ediati Sasmito Efendi Efendi Eka Kumalasari Endang Lukitaningsih Endang Lukitaningsih Endang Lukitaningsih Eny Dwi Astuti Febrianti, Dwi Rizki Gani, Andayana Puspitasari Gharsina Ghaisani Yumni Hifdzur Rashif Rija’i Ika Puspitaningrum Ika Puspitasari Indah Hastuti Ipang Djunarko Jovanita Fara Addina Kahfi Nur Fatimah Khoerul Anwar KRISNA KHARISMA PERTIWI Kusnanto, Chelvin Ari Laras Widawaty Putri Mar'uf, Muhammad Muhammad Hafizh Ridho Muthi Ikawati Ni'ma, Neli Syahida Niah, Rakhmadhan Nimas Ayu Kurniailla Nita Testikawati Nita Testikawati Normilawati Oktariani Pramiastuti Peni Susilowati Putri Peni Susilowati Putri, Peni Susilowati Puji Astuti Rahmawati, Adillah Ratih Anggar Kusumaningtyas Riris Istighfari Jenie Riski, Akhmad Rusydan, Azka M. Rusydan, Azka Muhammad Sari Rosiati Nur Khasanah Sigit Hartomo Siluh Astuti Siluh Astuti Siluh Putu Yuni Astuti Sitarina Widyarini Soda Farida Sofa Farida Sofa Farida Subagus Wahyuono Subagus Wahyuono Subagus Wahyuono Subagus Wahyuono Subagus Wahyuono Subagus Wahyuono Subagus Wahyuono Subagus Wahyuono Sutomo Sutomo Sutomo Sutomo T. N. Syaifullah Sulaiman Tahoangako, Sarmadhan Saputra Tatang Irianti Teuku Nanda Saifullah Sulaiman Tiaravista, Amanda Gita Widhihastuti, Endah Wirasti, Wirasti Yuli Widiyastuti Yuvianti Dwi Franyoto

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