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All Journal HAYATI Journal of Biosciences Jurnal Sain Veteriner El-Hayah : Jurnal Biologi Journal of Economics, Business, & Accountancy Ventura Biogenesis: Jurnal Ilmiah Biologi Prosiding Seminar Biologi Jurnal Manajemen Teori dan Terapan The Indonesian Biomedical Journal JBMP (Jurnal Bisnis, Manajemen dan Perbankan) BIOEDUKASI SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Biosaintifika: Journal of Biology & Biology Education Berkala Penelitian Hayati Exact Paper In Compilation (EPIC) Sewagati
Sri Puji Astuti Wahyuningsih, Sri Puji Astuti
Departemen Biologi, Fakultas Sains Dan Teknologi, Universitas Airlangga
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Okra Polysaccharides Improves Spleen Weight and B-Lymphocytes Proliferation in Mice Infected by Staphylococcus aureus Sri Puji Astuti Wahyuningsih; Manikya Pramudya; Intan Permata Putri; Nadyatul Ilma Indah Savira; Dwi Winarni; Listijani Suhargo; Win Darmanto
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11284

Abstract

Okra (Abelmoschus esculentus) is reported to have various biological functions such as antioxidant, anticancer and anti-inflammation. However, only few studies have been reported immunomodulatory activities of okra to prevent disease caused by bacteria infection. In this study, the immunomodulatory activities of polysaccharides from okra pods were investigated further through the spleen weight and B-lymphocytes proliferation in mice infected by Staphylococcus aureus. Okra polysaccharides were obtained by water extraction and ethanol precipitation. Okra polysaccharides with doses of 25, 50, 75, and 100 mg/kg BW were orally administrated to mice with or without Staphylococcus aureus infection. Spleen weight was evaluated in both treatment group and control group. B-lymphocytes proliferation was evaluated by MTT assay using LPS induction. Results showed that okra polysaccharide at the doses of 50, 75, and 100 mg/kg increased spleen weight (p 0.05) significantly. While at the dose of 75 and 100 mg/kg, it increased the B-lymphocytes proliferation (p 0.05) significantly. There is positive correlation between the spleen weight and B-lymphocytes proliferation by 73.3%. These result reveal that okra polysaccharide could improve the immune response and be utilized as a novel candidate of neutraceutical.
Potential of Red Okra Extract (Abelmoschus esculentus L. Moench) to Restore Kidney Damage due to Sodium Nitrite Sri Puji Astuti Wahyuningsih; Amalia Fachrisa; Nabilatun Nisa’; Baskara Wiku Adi Kusuma; Nadia Shoukat; Rasyidah Fauzia Ahmar; Na'ilah Insani Alifiyah
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.28141

Abstract

Sodium nitrite (NaNO2) found in vegetables, drinking water, and cured meats, can damage tissue because it is an oxidant. Plant phytochemicals such as quercetin are antioxidants. This study aimed to determine the potential of red okra pods ethanol extract (ROE) to repair kidney damage in mice (Mus musculus) induced by NaNO2. The red okra pods were extracted three times with saturated ethanol. The experiment used 36 male BALB/c mice aged 6-8 weeks and body weight of about 28 g. There are six research groups, namely, normal control, negative control (exposure to NaNO2 50 mg/kg BW), treatment of exposure to NaNO2 and administration of ROE at doses of 25, 50, 75, and 100 mg/kg BW. Sodium nitrite and ROE were given daily for 23 days by gavage. On day 24, the serum was isolated. Blood urea nitrogen (BUN) and creatinine (Cre) levels are measured to assess kidney function, as well as measuring the oxidant malondialdehyde (MDA) and the antioxidant enzyme of superoxide dismutase (SOD). The kidneys were made histological preparations and analyzed on the proximal convoluted tubule (PCT).  All data were statistically analyzed (α=0.05). This study indicated that the administration of ROE at a 100 mg/kg BW dose is the most optimal in repairing damage to the PCT with increased normal cells and reduced necrosis. Besides, it degraded BUN, Cre, and MDA levels in the serum of mice exposed to NaNO2 compared to the other treatments. All doses of ROE promoted the SOD level. ROE restore kidney tissue, especially on PCT to normal. Kidney damage due to exposure to NaNO2 preservatives can be reduced by administering ROE. ROE prevents kidney damage through an increase in antioxidant enzymes. ROE can be used as a food ingredient as a source of antioxidants, thereby reducing the impact of oxidant compounds.
Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis Sri Puji Astuti Wahyuningsih; Manikya Pramudya; Sugiharto Sugiharto
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.4969

Abstract

Mycobacterium tuberculosis is a major infection agent of tuberculosis that is controlled by the response of cell-mediated immunity. It is macrophages and cytolytic T lymphocytes. Activated macrophages will produce free radicals. Excessive free radicals cause tissue damage. Polysaccharide krestin contains β-glucan. It is a scavenger of free radicals. This research aimed to identify the influence of polysaccharide krestin from C. versicolor on nitrite and malondialdehyde concentrations of mice serum exposed by M. tuberculosis. Nitrite concentration was determined by nitrite assay. Malondialdehyde concentration was determined by TBARS assay. The result showed that adding polysaccharide krestin before exposure (P1) and adding polysaccharide krestin before-after exposure (P3) had the best potential to decrease nitrite concentration. Nitrite concentrations of P1 and P3 were 1.364 ± 0.523 M and 1.456 ± 0.712 M respectively. Meanwhile, P1 group and adding polysaccharide krestin after exposure (P2) had the best potential to decrease malondialdehyde concentration. Malondialdehyde concentrations of P1 and P2 were 1125.86 ± 97.96 µM and 953.86 ± 328.16 µM respectively. Their nitrite and malondialdehyde concentrations decreased, compared to K and K- groups. The research conclusion was that adding polysaccharide krestin before exposure could decrease both nitrite and malondialdehyde concentrations.How to CiteWahyuningsih, S., Pramudya, M., Sugiharto, S. (2016). Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis. Biosaintifika: Journal of Biology Biology Education, 8(1), 12-17.
Effect on Polysaccharide Krestin from Coriolus versicolor Extract on Phagocytic Activity and Capacity of Mus musculus Exposed by Pseudomonas aeruginosa Sri Puji Astuti Wahyuningsih; Nadyatul Ilma Indah Savira; Win Darmanto
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6957

Abstract

Pseudomonas aeruginosa is an opportunistic bacterium that causes infections in human. The wall cell of its bacteria contains lipopolysaccharide as virulency factors to protect it from human immunity. Lipopolysaccharide can inhibit phagocytosis in the body. Polysaccharide krestin (PSK) from Coriolus versicolor extract contains β-glucan that can increase phagocytic activity and capacity. This research aimed to identify the effect on polysaccharide krestin from C. versicolor extract on phagocytic activity and capacity of mice exposed by P. aeruginosa. The design of this research was experimental design. There were six treatment groups. The phagocytic activity and capacity were counted on slide smears of mice peritoneal fluid. The data was analyzed by using one way ANOVA. The results of the phagocytic activity and capacity showed that PSK was added before exposure (P1) or after exposure (P2) or before-after exposure (P3) had potential to increase phagocytic activity and capacity.  The conclusion of the research was that adding polysaccharide krestin either before exposure or after exposure or both of them could increase phagocytic activity and capacity. The benefits of this research to development of science are expected to reduce human infection and to utilize natural ingredients as immunomodulator.How to CiteWahyuningsih, S. P. A., Savira, N. I. I, Darmanto, W. (2016). Effect on Polysaccharide Krestin from Coriolus versicolor Extract on Phagocytic Activity and Capacity of Mus musculus Exposed by Pseudomonas aeruginosa. Biosaintifika: Journal of Biology Biology Education, 8(3), 308-313. 
Extract of Red Okra Pod (Abelmoschus esculentus L. Moench) Chemoprevents N-Methyl-N-Nitrosourea-Induced Kidney Proximal Tubular Cells Damage Achhlam, Divany Hunaimatul; Wahyuningsih, Sri Puji Astuti; Hapsari, Lukiteswari Dyah Tri; Soepriandono, Hari; Dewi, Firli Rahmah Primula
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1061-1070

Abstract

N-Methyl-N-Nitrosourea (MNU) is a compound that induces oxidative stress resulting in high levels of oxidants and damage to body cells. Red okra contains polyphenol and flavonoid active ingredients as antioxidants. This study aims to analyze the effect of ethanol extract of red okra pods (ROPE) on oxidant levels, antioxidant enzymes, and damage to rat kidney proximal tubule cells due to NMU. This study used 30 rats divided into six treatment groups, namely CN (normal), N (negative control; MNU 50 mg/kg BW), P (positive control; MNU and MTX 50 mg/kg BW), T1 (MNU and ROPE 50 mg/kg BW), T2 (MNU and ROPE 100 mg/kg BW), and T3 (MNU and ROPE 200 mg/kg BW). The treatment was carried out on all groups after eight weeks. The results indicate that malondialdehyde (MDA) and nitrogen oxide (NO) decrease with the ROPE treatment. The glutathione reductase (GSH) activity as an antioxidant enzyme increased T1 and T2, while glutathione peroxidase (GPx) showed an increase in T2 and T3. Furthermore, the biochemical marker of the rat kidney showed lower blood urea nitrogen (BUN) and creatinine (Cre) levels in all treatment groups. Then, the repair of damaged proximal tubule cells showed an increase in normal cells and lower swollen cells; however, there was a degradation in necrotic cells in T2 and T3. It can be indicated that the ROPE can act as an antioxidant that can reduce MDA and NO levels, increase GSH and GPx levels, and reduce damage to proximal renal tubule cells due to MNU.
Pelatihan Pembuatan dan Pemberian Probiotik dan Single Cell Protein pada Sapi Perah di Komunitas Peternak Junrejo, Kota Batu Amin, Muhammad Hilman Fu'adil; Wibowo, Anjar Tri; Supriyanto, Agus; Pratiwi, Intan Ayu; Wahyuningsih, Sri Puji Astuti; Pramudya, Manikya; Wahyuni, Dwi Kusuma; Junairiah, Junairiah; Dewi, Firli Rahmah Primula; Geraldi, Almando
Sewagati Vol 8 No 6 (2024)
Publisher : Pusat Publikasi ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j26139960.v8i6.2340

Abstract

Komunitas peternak sapi perah di Junrejo, Batu menghadapi tantangan dalam meningkatkan produktivitas dan kualitas susu akibat rendahnya kesehatan ternak dan keterbatasan akses terhadap teknologi pakan yang efisien. Program pengabdian masyarakat ini bertujuan untuk meningkatkan pemahaman dan keterampilan peternak dalam memanfaatkan probiotik dan Single Cell Protein (SCP) sebagai pakan tambahan, yang diharapkan dapat meningkatkan kualitas kesehatan dan produktivitas ternak. Program ini menggunakan pendekatan partisipatif yang melibatkan pelatihan langsung dan sesi diskusi untuk memastikan penerapan yang efektif dan berkelanjutan. Probiotik dan SCP berperan dalam memperbaiki mikrobiota usus ternak, meningkatkan efisiensi pencernaan, dan memberikan sumber protein berkualitas tinggi. Hasil pelatihan menunjukkan bahwa setelah mengikuti kegiatan ini, seluruh peserta (100%) memahami dasar-dasar probiotik dan SCP, serta mampu menerapkannya secara mandiri di peternakan mereka. Di samping itu, penggunaan molase sebagai media perbanyakan probiotik mendukung prinsip ekonomi sirkuler, mengurangi limbah produksi gula, dan menciptakan solusi pakan yang ramah lingkungan. Program ini berhasil meningkatkan kapasitas komunitas peternak lokal, sehingga dapat berkontribusi terhadap kesejahteraan ekonomi mereka melalui peningkatan hasil susu dan biaya operasional yang lebih efisien.
Feasibility of Probiotic Lactobacillus casei FNCC 0090 to Improve Growth and Immune Response of Catfish (Clarias gariepinus) toward Aeromonas hydrophila Infection Aini, Nurul; Wahyuningsih, Sri Puji Astuti; Fatimah; Andriyono, Sapto; Do, Hoang Dang Khoa; Bachruddin, Muhammad
HAYATI Journal of Biosciences Vol. 32 No. 4 (2025): July 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.4.931-939

Abstract

Aeromonas hydrophila can cause motile Aeromonas septicemia, leading to high mortality in catfish. An effective alternative to treat this bacterial infection is administering the probiotic Lactobacillus casei FNCC 0090. However, proper fisheries management is crucial for long-term success. The aim of this research is to determine the effect of administering the probiotic L. casei FNCC 0090 on the growth parameters and immune system of catfish. 200 catfish were divided into 5 different treatments in 5 replicates. The treatments were KN (negative control: without probiotic and infected), KP (positive control: without probiotic and not infected), A (5% probiotic and infected), B (10% probiotic and infected), C (probiotic 15% and infected). The density of probiotics administered is 108 CFU/ml. The fish were kept for 42 days; on the 35th day, the fish were infected with 1 x 108 CFU/ml A. hydrophila intramuscularly. The parameters observed in this study are fish growth (specific growth rate, SGR; feed conversion ratio, FCR; and survival rate, SR) and the immune system (phagocytic activity and the amount of lysozyme enzyme). The data obtained were analyzed with SPSS One Way ANOVA. The results showed that the administration of 15% probiotic L. casei FNCC 0090 had a significant effect on the growth parameters and immune system.
Potensi Ekstrak Ganoderma applanatum Terhadap Kadar MDA dan Bax pada Mencit (Mus musculus) yang Terinduksi Diethylnitrosamine Rahayu, Siti; Darmanto, Win; Wahyuningsih, Sri Puji Astuti
Exact Papers in Compilation (EPiC) Vol. 7 No. 2 (2025): Mei 2025
Publisher : Lembaga Penelitian Pengabdian Masyarakat Universitas KH. A. Wahab Hasbullah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32764/epic.v7i2.1514

Abstract

Polisakarida Ganoderma applanatum memiliki sifat antioksidan sebagai respon biologis yang kuat untuk terapi kanker. Penelitian ini menguji potensi ekstrak polisakarida G. applanatum terhadap kadar MDA dan Bax pada tikus (Mus musculus) yang diinduksi DEN. Tikus betina galur DDY dibagi secara acak menjadi 4 kelompok (n=6). DEN diberikan secara intraperitoneal dua kali seminggu, kemudian doxorubicin sebagai kontrol positif dan ekstrak polisakarida sebagai perlakuan selama satu bulan secara oral. Metode penelitian dilakukan dengan membandingkan KN yang diberi air; K- yang diberi DEN 100 mg/kg BB; K+ yang diberi DEN 100 mg/kg BB dan doksorubisin 10 mg/kg BB; dan P yang diberi DEN 100 mg/kg BB dan ekstrak polisakarida G. applanatum 150 mg/kg BB. Dari analisis statistik uji ANOVA yang dilanjutkan dengan uji Duncan, diperoleh bahwa pemberian ekstrak polisakarida G. applanatum dibandingkan dengan K- tidak menunjukkan perbedaan bermakna kadar MDA dan Bax. Data hasil percobaan sebagai berikut: KN menunjukkan kadar MDA sebesar 1,62±0,28 nmol/mL dan Bax 4,41±2,14 ng/mL. Kadar MDA K- sebesar 1,58±0,11 nmol/m dan Bax 3,91±1,84 ng/mL. Kadar MDA K+ sebesar 1,75±0,21 nmol/mL dan Bax 4,30±1,61 ng/mL. Kadar MDA P sebesar 1,74±0,29 nmol/mL dan Bax 2,28±1,27 ng/mL.
Antioxidant potential of ethanol, ethyl acetate, n-hexane extracts from leaves, fruits, stems, and roots of red okra (Abelmoschus esculentus) Putri, Salsabilla Wiyana; Hapsari, Lukiteswari Dyah Tri; Junairiah; Wahyuningsih, Sri Puji Astuti
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.43666

Abstract

Abelmoschus esculentus L. Moench has been known for its abundant and affordable antioxidant potential. This plant has different antioxidant levels regarding the parts of the plant. Red okra is rich in flavonoids and polyphenols, including strong antioxidant as quercetin ad anthocyanin. This research is intended to determine the IC50 value and compare the total content of phenolic and flavonoid from ethanol, ethyl acetate, and n-hexane extract of red okra (A. esculentus) leaves, fruits, stems, and roots. Extraction process was done by gradual maceration method using ethanol, ethyl acetate, and n-hexane. IC50 value interprets the antioxidant activity obtained from DPPH test. The results showed a very strong antioxidant activity from red okra stem extract with IC50 of 25.22 ppm and the lowest from fruit n-hexane extract with 821.55 ppm. From TPC test, the highest content was obtained from stem ethanol extract with 156.01 mg GAE/g and the lowest from stem n-hexane extract with 25.99 mg GAE/. From TFC test, the highest content was obtained from fruits ethanol extract with 108.15 mg QE/g and the lowest from stem ethanol extract with 2.26 mg QE/g. In conclusion, this research showed that red okra (A. esculentus) contains a high level of phenol and flavonoid compound and the highest antioxidant content was shown in ethanol extract.
Liposome-based Nanoparticles Encapsulating Vitamin D3 Attenuate IL-6 and TNF-α in a Menopausal Mouse Model Kusuma, Baskara Wiku Adi; Dwiningsih, Sri Ratna; As’adi, Ashon; Wahyuningsih, Sri Puji Astuti
The Indonesian Biomedical Journal Vol 17, No 5 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i5.3796

Abstract

BACKGROUND: Vitamin D3 is an essential regulator of immune function, however its bioavailability is limited. Liposomes as nanocarriers can enhance vitamin D3 absorption and delivery, however the application of liposomal vitamin D3 in postmenopausal remains underexplored, particularly in preclinical models. Estrogen deficiency during menopause promotes immune dysregulation and elevates proinflammatory cytokines, including interleukin (IL)-6 and tumor necrosis factor (TNF)-α. This study was conducted to evaluate the effects of liposomal vitamin D3 supplementation on serum vitamin D3, IL-6, and TNF-α levels in an ovariectomy-induced menopausal mouse model.METHODS: Mice were randomly divided into four groups comprising non-surgical control (N), ovariectomized without treatment (D−), conventional vitamin D3-treated (D+), and liposomal vitamin D3-treated (LD). Treatments were administered daily via oral gavage for two months. Serum vitamin D3, IL-6, and TNF-α levels were measured by enzyme-linked immunosorbent assay (ELISA). IL-6 and TNF-α data were analyzed by ANOVA with Duncan’s post-hoc test, while vitamin D3 data were analyzed using the Brown-Forsythe test with Games-Howell post-hoc test (p<0.01).RESULTS: Ovariectomy significantly decreased vitamin D3 levels and increased IL-6 and TNF-α levels in the D− group. Conventional vitamin D3 supplementation (D+) significantly decreased serum vitamin D3 levels and slightly decreased IL-6 and TNF-α levels. Liposomal vitamin D3 (LD3) significantly increased vitamin D3 levels and decreased TNF-α, only slightly decreasing IL-6. Correlation analysis showed a negative association between serum vitamin D3 levels and both cytokines.CONCLUSION: Administration of vitamin D3 liposomes was able to increase vitamin D3 levels and suppress IL-6 and TNF-α towards normal levels. LD3 offers enhanced bioavailability and anti-inflammatory effects, making it a promising therapeutic strategy for managing menopause-associated inflammation and related systemic disorders.KEYWORDS: menopause, liposomal VD3, inflammation, IL-6, TNF-α
Co-Authors Achhlam, Divany Hunaimatul Agus Supriyanto Ahmar, Rasyidah Fauzia Aini Nurul Alfiah Hayati Alifiyah, Na'ilah Insani Almando Geraldi Amalia Fachrisa Amalia Nur Mashita Andita Ayu Mandasari Anjar Tri Wibowo As’adi, Ashon Bachruddin, Muhammad Bambang Irawan Baskara Wiku Adi Kusuma Dewi Rahmawati Dita Wahyunita Do, Hoang Dang Khoa Dwi Kusuma Wahyuni Dwi Winarni Fachrisa, Amalia Fatimah Firli Rahmah Primula Dewi Hapsari, Lukiteswari Dyah Tri Hari Soepriandono Hastari Wuryastuti Himawan Sardju Indrianawati Usman Intan Ayu Pratiwi, Intan Ayu Intan Permata Putri Intan Permata Putri ISTUNING MA’UNAH Junairiah Junairiah Junairiah Kamiso H.N. Kushendarsasi Kusuma, Baskara Wiku Adi Lindiawati Lindiawati Listijani Suhargo, Listijani Manikya Pramudya Muhammad Hilman Fu'adil Amin Na&#039;ilah Insani Alifiyah Na'ilah Insani Alifiyah Nabilatun Nisa’ Nadia Shoukat Nadyatul Ilma Indah Savira Nisa’, Nabilatun Prenggono, Muhamad Darwin Putri, Salsabilla Wiyana R. Warsito Rasyidah Fauzia Ahmar Rasyidah Fauzia Ahmar S. Sugiharto Sapto Andriyono Shoukat, Nadia Siti Rahayu Sri Ratna Ningsih, Sri Ratna Sugiharto Suhendra Agung Wibowo Virid Gibson, Virid Vuanghao Lim Win Darmanto