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All Journal Majalah Kedokteran Gigi Indonesia Insisiva Dental Journal : Majalah Kedokteran Gigi Insisiva Dentino: Jurnal Kedokteran Gigi Journal of Indonesian Dental Association Dentin
Sherli Diana
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Dentistry

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DAYA HAMBAT EKSTRAK UBI BAWANG DAYAK (Eleutherine palmifolia (L.) Merr) TERHADAP PERTUMBUHAN Streptococcus mutans (Studi In Vitro Dengan Metode Difusi) Azilita Ananda; Deby Kania Tri Putri; Sherli Diana
Dentin Vol 2, No 1 (2018)
Publisher : Universitas Lambung Mangkurat

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Abstract

ABSTRAKLatar belakang: Karies merupakan penyakit kronis jaringan keras gigi yang salah satunya disebabkan oleh faktor mikroorganisme yaitu bakteri Streptococcus mutans, pertumbuhan bakteri ini dapat dihambat dengan memberikan ekstrak umbi bawang dayak. Umbi bawang dayak   merupakan tumbuhan herbal khas Kalimantan yang berpotensi sebagai alternatif obat kumur. Ekstrak umbi bawang dayak  memiliki kandungan yang bersifat antibakteri salah satunya adalah fenol sebagai kandungan terbesar dengan konsentrasi 34,20% yang dapat merusak sel bakteri  sehingga pertumbuhan Streptococcus mutans menurun dan lisis. Tujuan: Untuk mengetahui perbedaan daya hambat ekstrak umbi bawang dayak konsentrasi 80mg/ml dengan kontrol positif klorheksidin glukonat 0,2% terhadap pertumbuhan Streptococcus mutans. Metode dan bahan: Rancangan penelitian ini adalah true experimental design dengan post test only with control group. Penelitian ini menggunakan 6 kelompok perlakuan menggunakan sampel bawang dayak dengan metode maserasi dan pengujian aktivitas antibakteri dengan metode difusi.  Hasil penelitian: Nilai rata-rata zona hambat ekstrak umbi bawang dayak konsentrasi 20mg/ml sebesar 11,59 mm, konsentrasi 40mg/ml sebesar 14,39 mm, konsentrasi 60mg/ml sebesar 18,53 mm, konsentrasi 80mg/ml sebesar 23,55 mm, kontrol positif klorheksidin glukonat 0,2%  sebesar 21,39. Uji one-way Anova dan uji Post Hoc LSD menunjukkan bahwa terdapat perbedaan yang signifikan antara setiap kelompok perlakuan. Kesimpulan: Berdasarkan penelitian ini dapat disimpulkan bahwa terdapat perbedaan daya hambat ekstrak umbi bawang dayak konsentrasi 80mg/ml terhadap pertumbuhan Streptococcus mutans dengan zona hambat sebesar 23,55 mm dan klorheksidin glukonat 0,2% yang hanya memiliki zona hambat sebesar 21,39 mm. ABSTRACTBackground: Caries is a chronical disease of hard teeth tissue. It is caused by microorganism factor which is Streptococcus mutans bacterium, this bacterai can be inhibited with umbi bawang dayak extract . Umbi bawang dayak is Borneo particular herbal plant which has potential as an alternative to mouthwash. Umbi bawang dayak extracts contain antibacterial which have phenol as the largest content with 34.20% concentration. Purpose: To figure out the resistivity effect of umbi bawang Dayak extract with 20mg/ml, 40mg/ml, 60mg/ml and 80mg/ml concentration towards the growth of Streptococcus mutans. Method and Materials: This study applies a true experimental design with posttest-only with control group. This study takes six groups with 1 kg sampel of umbi bawang dayak using maserasi method and isolate of Streptococcus mutans using diffusion method. The Result of Research: The average number of inhibition zone of umbi bawang dayak extract with 20mg/ml concentration is 11.59mm, 40mg/ml concentration is 14.39mm, 60mg/ml concentration is 18.53mm, 80mg/ml concentration is 23.55mm. The average number of inhibition zone of umbi bawang dayak of chlorhexidine gluconate 0,2% is 21.39, and aquadest is 0.00mm. One-way Anova and Post-Hoc LSD show that there is significant difference between each of the treatment groups. Conclusion: Based on the result of the research, it can be concluded that there is different inhibition effect of umbi bawang dayak extract in 80mg/ml concentration with inhibition zone 23,55 mm and chlorhexidine gluconate 0,2%  with inhibition zone 21,39 mm towards the growth of Streptococcus mutans.
PENGARUH PAPARAN RADIASI TERHADAP pH SALIVA Nida Aulia; Bayu Indra Sukmana; Sherli Diana
Dentin Vol 5, No 3 (2021)
Publisher : Universitas Lambung Mangkurat

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Abstract

ABSTRACTBackground: The field of medicine is currently experiencing high progress, one of which is the radiography. The use of X-rays in medicine is as a diagnostic and therapeutic aid. X-rays can be used for diagnosis and treatment of patients. Therapy using radiation is called radiotherapy. Radiotherapy is one way of dealing with cancer, including cancer of the head and neck. The basic principle in radiotherapy for cancer or malignancy is to cause damage to every molecule that is exposed to radiation, resulting in the rupture of cell molecules so that cell damage occurs. Objective: To review various research articles related to the description of the effect of radiation on salivary pH. Methods: A review was conducted on 8 journals that were found to match the inclusion criteria such as journals available in full-text form and research subjects on the effect of radiation on salivary pH. Results: Based on 8 articles that have been reviewed, it was found that 8 articles (100%) stated that there was an effect of radiation on salivary pH after exposure to radiation after radiotherapy. The results showed that articles related to decreasing salivary pH stated that after being exposed to radiation after radiotherapy they tended to decrease salivary pH rather than increase it. Conclusion: there was a decrease in salivary pH after exposure to radiation. Keywords: Radiation, radiotherapy, salivary glands, salivary pH, X-rays. ABSTRAK Latar Belakang: Bidang kedokteran saat ini telah mengalami kemajuan yang tinggi, salah satunya bagian radiografi. Penggunaan sinar-X dalam bidang kedokteran adalah sebagai alat bantu diagnostik dan terapi. Sinar-X dapat dimanfaatkan untuk diagnosa maupun terapi pasien. Terapi dengan menggunakan radiasi disebut radioterapi. Radioterapi merupakan salah satu cara dalam usaha menanggulangi kanker, termasuk kanker pada bagian kepala dan leher. Prinsip dasar dalam radioterapi kanker atau keganasan adalah menimbulkan kerusakan pada setiap molekul yang terkena paparan sinar radiasinya mengakibatkan pecahnya molekul-molekul sel sehingga terjadi kerusakan sel.  Tujuan: Mengulas berbagai artikel penelitian yang berkaitan dengan gambaran pengaruh radiasi terhadap pH saliva. Metode: Review dilakukan pada 8 jurnal yang ditemukan sesuai dengan kriteria inklusi seperti jurnal tersedia dalam bentuk full-text dan subjek penelitian pengaruh radiasi terhadap pH saliva. Hasil: Berdasarkan 8 artikel yang telah dilakukan telaah, didapatkan bahwa 8 artikel (100%) menyatakan bahwa terdapat pengaruh radiasi terhadap pH saliva setelah terkena paparan radiasi pasca radioterapi. Hasil penelitian didapatkan bahwa artikel yang berkaitan dengan penurunan pH saliva menyatakan setelah terkena radiasi pasca radioterapi cenderung dapat menurunkan pH saliva daripada meningkatkan pH tersebut. Kesimpulan: Terjadi penurunan pH saliva setelah terkena paparan radiasi. Kata kunci: Kelenjar saliva, , pH saliva, radiasi, radioterapi, sinar-X.
PENGARUH JARAK PENYINARAN TERHADAP NILAI DERAJAT KONVERSI RESIN KOMPOSIT BIOAKTIF Ulfa Asma Wita Bancin; Isyana Erlita; Sherli Diana
Dentin Vol 6, No 2 (2022)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v6i2.6391

Abstract

ABSTRACTBackground: The improper irradiation process causes imperfect polymerization, so that the hardness of the composite resin, material strength, and color stability of the resulting composite resin are not optimal and water absorption increases. The long irradiation distance will result in the intensity of the light obtained getting smaller because the light can not reach the composite resin optimally and the degree of perfection of the polymerization of the composite resin will decrease. Objectives: The purpose of this study was to determine the difference between the irradiation distance and the degree of conversion of the bioactive composite resin and to analyze the ratio of the irradiation distance of 0 mm, 1 mm, and 5 mm to the value of the degree of conversion of the bioactive composite resin. Methods: This research uses a true experimental method with a post-test only design with a control group design with a simple random sampling technique. Results: The results showed that the highest degree of conversion of bioactive composite resins was at a 0 mm irradiation distance of 38.57%, 1 mm irradiation of 36.02% and the lowest was at a 5 mm irradiation distance of 31.90%. Conclusion: The highest degree of conversion of bioactive composite resin is at a 0 mm irradiation distance. Keywords: Beam distance, Composite resin, Degree of conversion,  Light intensityABSTRAKLatar belakang: Proses penyinaran yang kurang tepat menyebabkan polimerisasi tidak sempurna, sehingga kekerasan dari resin komposit, kekuatan bahan, stabilitas warna dari resin komposit yang dihasilkan tidak maksimal serta penyerapan air meningkat. Jarak penyinaran yang jauh akan mengakibatkan intensitas sinar yang didapat semakin kecil karena sinar tidak dapat mencapai resin komposit secara optimal dan derajat kesempurnaan polimerisasi resin komposit akan berkurang. Tujuan: Tujuan penelitian adalah untuk mengetahui perbedaan jarak penyinaran terhadap nilai derajat konversi resin komposit bioaktif serta menganalisis perbandingan jarak penyinaran 0 mm, 1 mm, dan 5 mm terhadap nilai derajat konversi resin komposit bioaktif. Metode: penelitan ini menggunakan metode eksperimental murni (true experimental) dengan rancangan post-test only with control group design dengan teknik pengambilan sampel simple random sampling. Hasil: Hasil penelitian menunjukkan nilai derajat konversi resin komposit bioaktif tertinggi berada pada jarak penyinaran 0 mm sebesar 38.57%, penyinaran 1 mm sebesar 36.02%  dan terendah berada pada jarak penyinaran 5 mm sebesar 31.90%. Kesimpulan: Nilai derajat konversi resin komposit bioaktif tertinggi berada pada jarak penyinaran 0 mm. Kata Kunci: Derajat konversi, Intensitas sinar, Jarak sinar, Resin komposit
HUBUNGAN PENGGUNAAN AIR MENGANDUNG MANGAN TERHADAP INDEKS DMF-T MASYARAKAT DI KECAMATAN DAHA SELATAN Muhammad Adeya Herdira Putra; Sherli Diana; Juliyatin Putri Utami
Dentin Vol 6, No 2 (2022)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v6i2.6396

Abstract

ABSTRACTBackground: Teeth and mouth problem ranks first in the top 10 disease that most Indonesian people complain. One of the factors that affect the caries index is the water used for daily. Water that contains metals can affect the caries index. There are several types of metals that can affect the DMF-T index, including manganese. Manganese can function as a caries causing agent. Objective: This study aims to measure the DMF-T index of people in Daha Selatan who use water containing manganese to brush their teeth. Methods: This research is an observational analytic design with a cross sectional approach. The subjects of this research are the 20-39 years people of South Daha. Measurement of water containing manganese was carried out in the laboratory and then assessed the DMF-T index to see the relationship between the use of water containing manganese and the incidence of dental caries. Results: The results showed that male respondents had a DMF-T index of 5.02 and a DMF-T index of people aged 30-39 of 5.53. The chi square correlation test showed that there was no relationship between the use of water containing manganese on the DMF-T index in Daha Selatan District. Conclusion: The majority of people aged 20-39 years in Daha Selatan District have a moderate DMF-T index and there is no significant relationship between the use of water containing manganese and the DMF-T index of the community in South Daha District.  Keywords: Caries, DMF-T Index, Manganese, WaterABSTRAK Latar Belakang: Penyakit gigi dan mulut manusia menduduki urutan pertama dari daftar 10 besar penyakit yang sering dikeluhkan masyarakat Indonesia. Salah satu faktor yang memengaruhi indeks karies yaitu air yang digunakan untuk sehari-hari. Air yang memiliki kandungan logam dapat memengaruhi indeks karies. Salah satu jenis logam yang dapat memengaruhi indeks DMF-T yaitu mangan. Mangan dapat berfungsi sebagai agen penyebab karies. Tujuan: Penelitian ini bertujuan untuk mengukur indeks DMF-T masyarakat di Kecamatan Daha Selatan yang menggunakan air mengandung mangan untuk menggosok gigi. Metode: Penelitian ini merupakan penelitian analitik observasional dengan pendekatan cross sectional. Sampel penelitian merupakan masyarakat di Kecamatan Daha Selatan yang berusia 20-39 tahun. Pengukuran sampel air mengandung mangan dilakukan di laboratorium selanjutnya melakukan penilaian indeks DMF-T untuk melihat hubungan antara penggunaan air mengandung mangan dengan kejadian karies gigi. Hasil:  Hasil penelitian menunjukkan responden berjenis kelamin laki laki memiliki indeks DMF-T sebesar 5,02 dan indeks DMF-T masyarakat berusia 30-39 sebesar 5,53. Uji korelasi chi square menunjukkan tidak terdapat hubungan penggunaan air mengandung mangan terhadap indeks DMF-T di Kecamatan Daha Selatan. Kesimpulan: Mayoritas masyarakat yang berusia 20-39 tahun di Kecamatan Daha Selatan memiliki indeks DMF-T kategori sedang serta tidak terdapat hubungan yang bermakna antara penggunaan air mengandung mangan terhadap indeks DMF-T masyarakat di Kecamatan Daha Selatan Kata Kunci: Air, Indeks DMF-T, Karies, Mangan
THE RELEASE OF FLUORIDE IONS OF BIOACTIVE RESIN IN THE SOLUTION OF LACTIC ACID AND ARTIFICIAL SALIVA Dewi Puspitasari; Nadya Fatimah Alzahrah; Indri Indah Tari; Diana Wibowo; Rahmad Arifin; Renie Kumala Dewi; Sherli Diana
Dentino : Jurnal Kedokteran Gigi Vol 7, No 2 (2022)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v7i2.14614

Abstract

Background: Bioactive resin can release fluoride ions when contact with oral environment. In the oral cavity, saliva and metabolic results of Streptococcus mutans namely lactic acid may affect the release of ions due to pH changes. Purpose: to analyse the release of fluoride ions of bioactive resin in the solution of lactic acid and artificial saliva. Methods: Forty-two specimens (diameter 15 mm x thickness 1 mm; n= 7/group fabricated with Activa™ Bioactive Restorative (Pulpdent). The specimen swere randomized into six 6 groups which immersed in lactic acid, artifial saliva and distilled water for 1 and 7 days in the incubator at 37oC. The number of fluoride ion release was measured using pH Meter (Lutron-208). Results: Two Way Anova test and Post Hoc Bonferonni test show there are significant differences among all group for lactic acid 1 day (6.37 ± 0.74), artificial saliva 1 day (1.30 ± 0.48), distilled water 1 day (3.78 ± 0.97), lactic acid 7 days (22.9 ± 0.81), artificial saliva 7 days (19.00 ± 0.81), distilled water 7 days (21.60± 1.01) ppm. Conclusion: The fluoride ion release of bioactive resin which immersed in lactic acid solution was higher than in artificial saliva immersion.  Keywords: Artificial saliva, Bioactive resin, Fluoride ion release, Lactic acid
ANTIBACTERIAL ACTIVITY OF RAMBAI LEAF EXTRACT (Sonneratia caseolaris) CONCENTRATION 25% 50% 75% AND 100% AGAINST BACTERIA Enterococcus faecalis US Ramadilla Ramadilla; Didit Aspriyanto; Sherli Diana
Dentino : Jurnal Kedokteran Gigi Vol 8, No 1 (2023)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v8i1.16076

Abstract

ABSTRACTBackground: The causative factor of failure in the treatment of the root canal is the presence of microorganisms, namely the bacterium Enterococcus faecalis. Rambai leaf extract (Sonneratia caseolaris) also contains secondary metabolite compounds, namely, triterpenoids, phenols, tannins, steroids, and flavonoids that are able to inhibit the growth of Enterococcus faecalis bacteria which have the potential to cause failure in root canal treatment. Purpose: This research has a purpose to see the antibacterial activity of Rambai leaf extract (Sonneratia caseolaris) concentrations of 25%, 50%, 75%, and 100% against Enterococcus faecalis bacteria. Method: This study is a true experimental study designed by Posttest Only with Control Group Design using six. Material and methods: Rambai leaf extract concentrations of 25%, 50%, 75%, 100%, positive control of chlorhexidine gluconate 2%, and negative control of aqua dest was repeated 4 times. Antibacterial activity can be seen from the magnitude of the inhibitory zone formed in (MHA) Mueller Hinton Agar and BHIB (Brain Heart Infusion) using the diffusion method. Results: Based on Mann Whitney's final test, there was a significant difference in the clear zones formed around the paper disk. The average clear zone of Rambai leaf extract (Sonneratia caseolaris) concentration is 25% at 10.0mm, 50% at 12.7mm, 75% at 17.0mm, and 100% at 18.3mm. Conclusion: There is activity of Rambai leaf extract (Sonneratia caseolaris) concentrations of 25%, 50%, 75%, and 100% against Enterococcus faecalis bacteria. Keywords: Antibacterial Activity, Enterococcus faecalis, Rambai Leaf Extract
THE EFFECT OF MAULI BANANA (Musa acuminata) STEMS AND BASIL LEAVES (Ocimum basilicum) EXTRACTS ON GIC DISCOLORATION Norhayati Norhayati; Sherli Diana; Rosihan Adhani
Dentin Vol 7, No 2 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v7i2.9718

Abstract

Background: The water settable GIC material is easily influenced by fluids and is hydrophilic which causes discoloration of the restoration. Chlorhexidine is the gold standard mouthwash and if used for a long period of time it can cause discoloration of the restoration because it contains chemicals, so an alternative mouthwash made from natural ingredients is needed. A mixture of mauli banana stem and basil leaves extract has a more optimal antibacterial ability. Objective: In general, this study aimed to analyze the effect of a mixture of Mauli banana stem (Musa acuminata) and basil leaves (Ocimum basilicum) extracts on discoloration of the water settable GIC. Methods: This research is a true experimental research with pre test and post test designs with control group design. This study was divided into three treatments, namely water settable GIC soaked in a mixture of extracts of banana stems and basil leaves at concentrations of 75%, 100%, and 0.2% chlorhexidine gluconate (positive control). Color measurement was carried out using a series of digital analysis tools consisting of a Samsung A1 macro camera, Peyond series PD-480 SL and the MATLAB application. Results: The results of the Kruskal-Wallis statistical test showed p value = 0.282 (> 0.05), meaning that there was no significant difference in color change between the three treatments. Conclusion: There is an effect of a mixture banana mauli stems (Musa acuminata) and basil leaves (Ocimum basilicum) extracts at concentrations of 75%, 100%, and 0.2% chlorhexidine gluconate on the discoloration of the water settable GIC. Keywords: Basil leaves (Ocimum basilicum), CIELAB system, discoloration, mauli banana stem (Musa acuminata), water settable GIC
THE EFFECTS OF SOAKING EXTRACTS OF STEMS Musa Acuminate AND LEAVES Ocimum Basilicum L. ON FLUORIDE RELEASE OF WATER SETTABLE GIC Gusti Wina Ayu Hazarisa; Sherli Diana; Nurdiana Dewi
Dentin Vol 7, No 2 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v7i2.9724

Abstract

Background: Mouthwash is a dental and oral health product that is easy to obtain and practical to use. Mouthwash is able to kill bacteria causing dental and oral health problems. Mauli banana stems and Basil leaves can be used as an alternative to natural mouthwash because they contain bioactive compounds such as saponins, alkaloids, lycopene, ascorbic acid, flavonoids, and tannins. Objective: To analyze the effect of a mixture of extracts of Mauli banana (Musa acuminate var. sapientum) stems and Basil leaves (Ocimum basilicum L.) at concentrations of 75% and 100% on fluoride ion release in the Water Settable GIC. Methods: Pure experimental design with post-test only with control group design. The number of samples consisted of 21 samples, which were divided into 3 treatment groups, and then the value of fluoride ion release was measured after 22 hours. Results: One Way Anova test showed a significant difference with p <0.05 among the treatment groups. The results of the Post Hoc Bonferroni test obtained a p-value of <0.05, which means that there was a significant difference between the control group compared to the extracts of Mauli banana stems and Basil leaves at concentrations of 75% and 100%. Conclusion: There was an effect of the mixture of the extracts of Mauli banana (Musa acuminate var. sapientum) stems and Basil leaves (Ocimum basilicum L.) at concentrations of 75%, 100%, and artificial saliva on fluoride ion release in the Water Settable GIC. Keywords: Basil Leaves, Fluoride Ion, Mauli Banana Stems, Water Settable.
EFFECT OF ROBUSTA COFFEE BEANS AND ARABICA EXTRACT GEL AS INFLAMATION PULP MATERIAL Sherli Diana; Nadiya Amalia; Deby Kania Tri Putri
Dentino : Jurnal Kedokteran Gigi Vol 8, No 2 (2023)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v8i2.17531

Abstract

Background: Reversible pulpitis is a mild to moderate inflammatory pulp condition often treated with pulp capping treatments. Current study states that calcium hydroxide (CaOH2) has drawbacks for a long-time use. One of the alternative pulp capping materials is Robusta and Arabica coffee bean extract gel which contains flavonoid compounds and chlorogenic acid which have immunomodulatory properties that play role in the pulp inflammatory process by increasing the count of lymphocyte. Objective: The goal of this study is to determine and analyse the effect of Robusta coffee bean and Arabica coffee bean extract gel 95% to the of lymphocyte cells count on the 3rd and 5th day of pulp inflammation compared to placebo gel. Materials and Methods: This research is a true experimental with a post-test only control group design. This study used three treatment groups; Robusta coffee bean extract gel 95%, Arabica coffee bean extract  gel 95%, and control group placebo gel. Results: Two Way Anova test results obtained p<0.05, showed significant difference. Data analysis was continued with the Post Hoc Bonferroni test which showed that there was a significant difference between the 95% Robusta coffee bean extract gel group and the 95% Arabica coffee bean extract gel group with p<0.05. Conclusion: The administration of Robusta coffee bean extract gel with a concentration of 95% can increase the of lymphocyte cells count in pulp inflammation on the 3rd and 5th days more effectively than the 95% Arabica coffee bean extract gel. Keywords: Arabica Coffee, Direct Pulp Capping, Inflammation, Lymphocytes, Pulpitis, Robusta Coffee
UJI TOKSISITAS EKSTRAK DAUN GALAM (Melaleuca cajuputi subsp. Cumingiana Barlow) PADA BHK-21 SEL FIBROBLAS Brachmedio Barito Syech Erlangga; Sherli Diana; Debby Saputera; Didit Aspriyanto; Beta Widya Oktiani
Dentin Vol 7, No 3 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v7i3.10743

Abstract

Latar Belakang: Karies gigi merupakan penyakit rongga mulut yang salah satu faktor penyebabnya adalah bakteri. Salah satu bahan alam yang berpotensi sebagai antibiotik dan hidup di lingkungan lahan basah adalah tanaman galam (Melaleuca cajuputi subsp. Cumingiana Barlow). Fungsi uji toksisitas adalah untuk mengetahui efek toksik dan batas dosis aman suatu senyawa kimia dalam penelitian ini pengujian ekstrak daun galam. Tujuan: Menganalisis efek toksik setelah pemberian ekstrak daun galam (Melaleuca cajuputi subsp. Cumingiana Barlow) terhadap sel fibroblas BHK-21. Metode: Penelitian ini adalah penelitian eksperimental murni (true experimental) dengan rancangan posttest-only with control group design untuk menganalisis toksisitas ekstrak daun galam terhadap sel fibroblas Baby Hamster Kidney-21 (BHK-21) dengan metode Microculture Tetrazolium Technique (MTT) assay secara in vitro. Hasil: Hasil penelitian menunjukkan bahwa ekstrak daun galam tidak toksik karena viabilitas sel pada semua konsentrasi >100% dan nilai IC50 yang tidak mungkin tercapai. Hasil uji post hoc Games-Howell menyimpulkan bahwa konsentrasi 50%, 75%, dan 100% lebih efektif daripada konsentrasi 0,125%, 0,2%, 0,25%, dan 0,4%. Kesimpulan: Tidak ada efek toksik ekstrak daun galam dengan uji MTT terhadap sel fibroblas BHK-21.Kata kunci: ekstrak daun galam, sel fibroblas BHK-21, toksisitas
Co-Authors Afiati, Risti Agung Pratama Akbar Agung Satria Wardhana Alexander Sitepu Alya Rahmasari Amina Khairima Amy Nindia Carabelly Andi Triawan, Andi Aqshall Ilham Safatullah Arifin, Rahmad Aspriyanto, Didit Aulia Azizah Azilita Ananda Bayu Indra Sukmana Beta Widya Oktiani Brachmedio Barito Syech Erlangga Buyung Maglenda Citra Aghnia Thamara Debby Saputera, Debby Dewi Nurdiana Dewi Puspitasari Dewi Puspitasari Dewi, Renie Kumala Diana Wibowo Diyah Ayu Rizki Tiara Defi Dwi Kurniawan, Fajar Kusuma Erine Febrianti Fathonah, Zeni Dwi Fitri Kabeakan Gunawan Halim, Novia Gusti Wina Ayu Hazarisa Hanifah Mulyani Hatta, Isnur Husma, Emma Annahal Ichrom Nahzi, Muhammad Yanuar Ika Kusuma Wardani Indri Indah Tari Irpansyah Irpansyah Karina Ramadhani Kevinda Januarizqi Khairima, Amina Khairina, Niketa Khairunnisa Khairunnisa Khairunnisa Khairunnisa Maharani Laillyza Apriasari Muhammad Adeya Herdira Putra Muhammad Rezky Gunawan Nadiya Amalia Nadya Fatimah Alzahrah Nida Aulia Niketa Khairina Norhayati Norhayati Noval Ihza Maulana Novia Gunawan Halim Nur As Alifuddin Nurrahman, Tri Pribadi Santosa Putra, Fedriko Gamaliel R. Harry D. Setyawardhana Rahmad Arifin Rahmatillah Rahmatillah Raihatun Nida Ramadhani, Karina Ratih Yusnita Reni Amirah Salsabila Fitri Renie Kumala Dewi Resha Yusnida Risti Afiati Rizki Sri Yuliati Rosihan Adhani, Rosihan Sarifah, Norlaila Setyawardhana, Raden Harry Dharmawan Sinaga, Gloria Meyana Sitepu, Alexander Thamara, Citra Aghnia Tri Putri, Deby Kania Ulfa Asma Wita Bancin US Ramadilla Ramadilla Utami, Juliyatin Putri Yudha Fatahillah Syahari Yusrinie Wasiaturrahmah