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All Journal Journal of Tropical Biodiversity and Biotechnology BIOEDUSCIENCE Jurnal Biologi Tropis Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi Jambura Edu Biosfer Journal Agroteknika Jurnal Ilmiah Biologi UMA (JIBIOMA) Jurnal Sibermas (Sinergi Pemberdayaan Masyarakat) Hydrogen: Jurnal Kependidikan Kimia Bioscientist : Jurnal Ilmiah Biologi BIOVALENTIA: Biological Research Journal Lamahu: Jurnal Pengabdian Masyarakat Terintegrasi JURNAL SIPISSANGNGI: Jurnal Pengabdian Kepada Masyarakat Journal of Applied Community Engagement (JACE) Filogeni: Jurnal Mahasiswa Biologi Journal of Tropical Mycorrhiza Jurnal Abdimas Terapan Biodik: Jurnal Ilmiah Pendidikan Biologi Science: Indonesian Journal of Science Damhil: Jurnal Pengabdian kepada Masyarakat Bioma : Jurnal Biologi dan Pembelajaran Biologi Inventa: Journal of Science, Technology, and Innovation
Devi Bunga Pagalla
Universitas Negeri Gorontalo
Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Other

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Induksi Kalus dari Antera Pisang Goroho (Musa acuminata L.) dengan Menggunakan 2,4-D dan BAP Risna A. Onu; Jusna Ahmad; Febriyanti Febriyanti; Novri Youla Kandowangko; Devi Bunga Pagalla; Indriati Husain
Journal of Science, Technology, and Innovation Vol 1 No 2 (2025): December: Inventa: Journal of Science, Technology, and Innovation
Publisher : CV SCRIPTA INTELEKTUAL MANDIRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.65310/8t6ph238

Abstract

Callus induction is a critical stage in anther culture for supporting the propagation and genetic improvement of local banana cultivars. This study evaluated the effect of different combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP) on callus induction from goroho banana (Musa acuminata L.) anthers. Observations were conducted for 30 days after induction, focusing on callus emergence time and morphological characteristics, including color and texture. The results demonstrated distinct responses among treatments. The combination of 1.5 ml 2,4-D and 1.5 ml BAP produced the fastest callus initiation with an average of 13.17 days after induction and generated friable callus with a pale yellow coloration, indicating high cellular activity. Lower growth regulator concentrations delayed callus formation, while higher concentrations promoted color changes associated with early tissue differentiation. No callus formation was observed in the absence of exogenous growth regulators. These findings highlight the importance of balanced auxin and cytokinin concentrations in regulating anther-derived callus induction and provide a physiological basis for further development of goroho banana anther culture systems.
Influence of Various Types of Chitosan on Biomass and Callus Morpology of Momordica Cochinchinensis in Vitro Nazwa S. Lamante; Devi Bunga Pagalla; Jusna Ahmad; Novri Youla Kandowangko; Indriati Husain
Jurnal Biologi Tropis Vol. 26 No. 3 (2026): Juli - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i3.12314

Abstract

Momordica cochinchinensis is a medicinal, herbs locally known in gorontallo as dumbaya. Tradisionally, this plant has been utilized as both a food of food course a herbal medicine. Due to its rich contains of bioactive compounds, making it a potential candidate for development through plant tissue culture. This study aimed to determine the effects of different chitosan concentrations on the biomass and callus morphology of M. cochinchinensis cultivated in vitro. The experiment was arranged in a non-factorial completey randomized design consisting of three chitosan concentrations: 15 mg L -1, 25 mg L-1, and 35 mg L-1, respectively, as well as a 5000 mg L-1 chitosan stock solution per liter of MS medium. Each treatment consisted of three replicates. The observed parameters included callus fresh weight, dry weight, color, texture, and the percentage of callus formation. Data were analyzed using a one-way ANOVA at a 5% significance level. Since the fresh weight data showed significant differences, it was followed by a Duncan’s Multiple Range Test (DMRT) at the 5% level. The results showed that different chitosan concentrations significantly affected the callus fresh weight (F = 7.932; p = 0.021). the 15 mg L-1 treatment yielded the highest fresh weight, 0.04067 ± 0.0115 g, which was significantly different from the 35 mg L-1 treatment (0.3000 ± 0.0458 g) but not significantly different from the 25 mg L-1 treatment (0.3300 ± 0.0346 g) conversely, chitosan concentration did not significantly affect the culls dry weight (F = 3.519); p = 0.097). all treatmeants resulted in 100% callus formation with white and friable callus morphology. These findings indicate that among the tasted concentrations, 15 mg L-1 of chitosan is the most favorable concentration for demonstrating callus fresh weight accumulation of M. cochinchinensis under in vitro culture conditions.
Effect of Methyl Jasmonate on In Vitro Callus Growth of Momordica cochinchinensis Widyawati S. Isima; Jusna Ahmad; Devi Bunga Pagalla; Novri Youla Kandowangko; Indriati Husain
Jurnal Biologi Tropis Vol. 26 No. 3 (2026): Juli - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i3.12324

Abstract

Momordica cochinchinensis (Lour.) Spreng., locally known as dumbaya in Gorontalo, is a tropical climbing plant with potential as a functional food and medicinal resource, but its natural availability is limited by low seed germination, slow growth, and environmental sensitivity. This study aimed to determine the effect of methyl jasmonate (MeJA) concentrations on callus growth of young stem explants of M. cochinchinensis under in vitro conditions. The experiment used a completely randomized design with five treatments: MS without plant growth regulators, MS + 2,4-D + BAP, MS + MeJA 6 mL/L, MS + MeJA 11 mL/L, and MS + MeJA 16 mL/L. The observed parameters were callus formation percentage, callus initiation time, and callus morphology. Quantitative data were analyzed using one-way ANOVA followed by Duncan’s test at 5%, while morphological data were described qualitatively. All treatments produced 100% callus formation within 14 days. MeJA affected callus initiation time, with MS + MeJA 6 mL/L showing the fastest average response at 3.67 days after culture. The callus was mostly friable with varied coloration. This treatment may support further development of M. cochinchinensis callus culture and provides preliminary information for optimizing in vitro propagation protocols for this locally valuable species in future studies.
Co-Authors Abubakar Sidik Katili Adelia Salsabila Rahman Adilah Nidaulhasanah Adju, Fitria Y.Hs. Ahaya, Cindi Ahmad Hidayat Aisyah Ahmad Andi Nursanti Ari Indrianto Arviani Arviani Aulia Rahmadani Chairunnisah J Lamangantjo Chairunnisah J Lamangantjo Endang Semiarti Evita Moni Damayanti Fadlia Dunggio Febriyanti Febriyanti febriyanti febriyanti Fitria H.Ys. Adju Haerani Haerani Hartono D Mamu Hartono D. Mamu Hayati Ningsih Gubali Hayatiningsih Gubali Herinda Mardin Indayani Tandililing Indiriati Husain Indriati Husain Isima , Lindawaty Jusna Ahmad Lilan Dama M. Irfan Magfirahtul Jannah Maryani Maryani Masra Latjompoh Masrid Pikoli Menanti, Menanti Miftahul F. Adudu Muhammad Isra Mustaqimah, Nur Najmah Najmah Najmah, Najmah Nazwa S. Lamante Novri Youla Kandowangko Novri Youla Kandowango Nur Mustaqimah Nurhayati Nurhayati Nurhayati Nurhayati Nurman Nurman Nurul Fajryani Usman Nurul Iqraini D Rahmah, Mufti Hatur Ramadhani, Andi Indra Wulan Sari Ramlah Ramlah Ramlah Ramlah Rasydah Nur Tuada Restiyani Widia Putri Retno Sari Rika Eyato Risna A. Onu Sangkota, Vivi Dia Afrianti Sintia S. Ahmad St Hijrah Alfiani Tri Vinesa Moha Walangadi, Farhana Rahmatia Widyawati S. Isima Wirnangsi Din Uno Wirnangsih D. Uno Yuliana Retnowati