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All Journal EJIP BIOL Jurnal Penelitian dan Pengembangan Pendidikan Jurnal Pendidikan Indonesia Journal of Educational Science and Technology Biology, Medicine, & Natural Product Chemistry Promotif: Jurnal Kesehatan Masyarakat Jurnal Penelitian Pendidikan IPA (JPPIPA) JIIP (Jurnal Ilmiah Ilmu Pendidikan) Jurnal Abdidas Ideguru: Jurnal Karya Ilmiah Guru Jurnal Riset Pendidikan MIPA Bioscientist : Jurnal Ilmiah Biologi Biology and Education Journal (BaEJ) Equator Science Journal (ESJ) J-SES : Journal of Science, Education, and Studies Bioma : Jurnal Ilmiah Biologi Journal of Biology Science and Education Biodiversitas Journal of Biological Diversity
Sutrisnawati Mardin
Program Studi Pendidikan Biologi, Fakultas Keguruan Dan Ilmu Pendidikan, Universitas Tadulako
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Molecular detection and phylogenetic analysis of Schistosoma japonicum in Central Sulawesi, Indonesia using COI gene SUTRISNAWATI SUTRISNAWATI; ACHMAD RAMADHAN
Biodiversitas Journal of Biological Diversity Vol. 26 No. 8 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260833

Abstract

Abstract. Sutrisnawati, Ramadhan A. 2025. Molecular detection and phylogenetic analysis of Schistosoma japonicum in Central Sulawesi, Indonesia using COI gene. Biodiversitas 26: 4042-4047. Schistosomiasis, an endemic disease in several tropical regions, including Indonesia, is caused by Schistosoma japonicum, making its early detection crucial for controlling the spread. Molecular detection using the Cytochrome Oxidase 1 (COI) gene not only aids in diagnosing infections but also contributes to environmental DNA (eDNA) studies by detecting parasite DNA from environmental samples such as water or soil. This study aimed to detect Schistosoma japonicum in Central Sulawesi, Indonesia, using the COI gene as the molecular target. Fecal samples from individuals suspected of infection were collected from various endemic regions in the area. The Polymerase Chain Reaction (PCR) technique successfully amplified a 585 bp fragment of the COI gene, considered both sensitive and specific for S. japonicum detection. Out of the five samples analyzed, a number tested positive, and the resulting amplicons showed 99.21-99.52% sequence similarity with S. japonicum sequences in the GenBank database. Phylogenetic analysis placed these isolates within a well-supported clade together with S. japonicum from other parts of Indonesia and neighboring countries, confirming their close evolutionary relationship. Minor nucleotide variations were observed, suggesting potential local adaptations that may have epidemiological relevance. These results confirm the presence of S. japonicum in Central Sulawesi and demonstrate the utility of the COI gene in both detection and genetic characterization. The findings also highlight the value of incorporating molecular data into schistosomiasis surveillance to improve risk mapping, guide intervention priorities, and monitor possible drug resistance emergence. This approach complements traditional diagnostic methods, which often have reduced sensitivity in light infections. In conclusion, the COI gene provides a reliable tool for identifying and understanding the genetic diversity of S. japonicum in Indonesia. Future research should expand sample sizes and geographic coverage to further elucidate the population structure of S. japonicum and support more targeted, sustainable schistosomiasis control strategies locally and globally.
Abundance of Bivalves in the Mangrove Ecosystem of Pinotu Village, Toribulu District, Parigi Moutong Regency Muslika Nifatun Umaya; Fatmah Dhafir; Abd Rauf; Syech Zainal; Rafiqa Rafiqa; Sutrisnawati Sutrisnawati; Manap Trianto
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1415-1421

Abstract

Bivalves are one of the molluscan groups that play an important role in maintaining the ecological balance of mangrove ecosystems. Their presence and abundance are influenced by environmental conditions, making them useful indicators of habitat quality. This study aimed to determine the abundance of bivalves in the mangrove ecosystem of Pinotu Village, Toribulu District, Parigi Moutong Regency, Central Sulawesi, Indonesia. A quantitative descriptive approach was employed in this study. The research sites were selected using a purposive sampling technique based on the presence of suitable bivalve habitats. Sampling was conducted using the belt transect method at two observation stations, each covering an area of 500 m². Field sampling was carried out during low tide on 17–18 December 2025. Collected bivalve specimens were identified using taxonomic identification keys. In addition, environmental parameters, including water temperature, salinity, and pH, were measured. The collected data were analyzed using the species abundance index (R) and the Relative Abundance Index (RAI). The results revealed the presence of four bivalve species belonging to two orders and two families, namely Polymesoda erosa (39 individuals), Polymesoda expansa (19 individuals), Polymesoda bengalensis (38 individuals), and Anadara granosa (9 individuals), with a total of 105 individuals recorded. The species abundance index (R) value of 0.64 indicated a low level of bivalve abundance. The highest Relative Abundance Index values were observed for Polymesoda erosa (37.14%) and Polymesoda bengalensis (36.19%), both classified as highly abundant. In contrast, Anadara granosa exhibited the lowest relative abundance value (8.57%) and was categorized as having low abundance.
Pengembangan Modul Ajar berbasis STEAM-Inkuiri untuk Meningkatkan Minat Belajar dan Keterampilan Berpikir Kritis Peserta Didik di SMK Kesehatan Nusantara Palu Farhana Lembah; Achmad Ramadhan; Sutrisnawati
Jurnal Pendidikan Indonesia Vol 15 No 1 (2026): March
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jpi-undiksha.v15i1.104132

Abstract

Learning in vocational high schools (SMK) in the health field still faces limitations in contextual and interactive teaching materials, which negatively affect students’ learning interest and critical thinking skills, particularly in Human Anatomy and Physiology topics. This study aimed to develop, validate, and evaluate a STEAM–Inquiry-based learning module to enhance students’ learning interest and critical thinking skills in health vocational schools. The study employed a Research and Development (R&D) method using the 4-D model (Define, Design, Develop, and Disseminate), which was limited to the Develop stage. The research subjects consisted of 29 eleventh-grade students selected through purposive sampling. The research instruments included a critical thinking skills rubric, a learning interest questionnaire, and expert validation sheets. Data were analyzed using descriptive statistics and N-Gain tests. The results indicated that the STEAM–Inquiry-based module achieved a very high feasibility level (>76%) and significantly improved students’ learning interest and critical thinking skills, with an N-Gain score of 0.71. The module was found to be valid, practical, and effective in promoting active, creative, and critical learning. The implications of this study suggest that the STEAM–Inquiry-based module can serve as an innovative alternative teaching material in health vocational schools to support the implementation of the Merdeka Curriculum and to strengthen 21st-century learning oriented toward critical thinking and problem-solving skills.
WIELDING INTERACTIVE FLAT PANELS (IFP) TO ENRICH STUDENTS’ VOCABULARY: A CLASSROOM ACTION RESEARCH Rizal Wahid Permana Putra; Achmad Ramadhan; Sutrisnawati Sutrisnawati
J-SES : Journal of Science, Education and Studies Vol 5 No 2 (2026): Vol 5 No 2 (2026): Jurnal J-SES Vol 5 No 2
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jses.v5i2.31487

Abstract

This Classroom Action Research (CAR) investigates the integration of Interactive Flat Panels (IFPs) to enhance vocabulary acquisition among seventh-grade students at SMP Negeri Satu Atap Gondoruso 01. The study addressed low student motivation and initial proficiency levels linked to conventional pedagogical approaches. Following the Kemmis and Taggart model, the research was conducted in 2 cycles involving 31 students. Data were collected through multiple-choice tests, observations, and field notes. Results indicated a steady quantitative improvement: the baseline pre-test average of 65.80 rose to 68.70 in Cycle I and culminated in 74.03 in Cycle II, shifting students from "fair" to "moderate" proficiency. The findings demonstrate that transitioning IFP usage from a passive visual aid to an active cognitive tool—incorporating digital scaffolding, "Sentence Train" activities, and immediate corrective feedback—effectively bridges the gap between physical engagement and language retention. Consequently, the shared-intellectual space created by IFPs successfully lowered affective filters and fostered a dynamic, collaborative learning environment, and improved students' vocabulary. Keywords: Interactive Flat Pannels; Vocabulary Acquisition; Classroom Action Research
The Effect of Brain Based Learning Approach on Science Process Skills and Learning Motivation of Fifth Grade Elementary School Students Surahmin; Mustapa, Kasmudin; Sutrisnawati
Jurnal Universitas Tadulako Vol. 9 No. 2 (2025)
Publisher : FKIP Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/j25490192.2025.v9.i2.pp70-76

Abstract

This study aims to describe the science process skills and motivation of students' learning skills by applying the brain based learning model. This research is a quasi-experimental research. The population consisted of all students of SD Inpres Boyaoge and a sample of grade 5a and 5b students. The science process skills instrument used a student activity sheet test and motivation used a questionnaire. The data were then analyzed using the Manwhitney test. The results showed that there was an effect of the learning model on students' science process skills with an average BBL class of 94.48% as well as on students' learning motivation with an average of 90%, these results can be used as an alternative to improve science process skills and motivation in students.
Co-Authors Abd Rauf Abd Rauf Abd. Hakim Laenggeng Abd. Hakim Laenggeng Abd. Hakim Laenggeng Abd. Hakim Laenggeng, Abd. Hakim Abd. Rauf ACHMAD RAMADHAN Achmad Ramadhan Achmad Ramadhan Achmad Ramadhan Achmad Ramadhan Achmad Ramadhan Adi Fahri Ahmad Ramadhan Alya Shafira Nurhafizhah Amelia A Riuh Amiruddin Kasim Angriani, Novita Ashari, Abdul Astija Baehaqi Balebu, Dwi Wahyu Bustamin Bustamin Bustamin Desak Putu Widya Apriliani Dinie, Norhamizah Dwi Setyorini Elfandi, Moh. Aziz Erna, Ni Kadek Estevania Angelin Lago Faradila, Ratna Farhana Lembah Fariza, Ifat Fatmah Dhafir H. Achmad - Ramadhan, H. Achmad - Hayyatun Mawaddah Henny Chandra I Ketut Suada I Made Budiarsa I Nengah Kundera I Nengah Kundera Ida Nur Aeni, Ida Nur Illa Faradila Putri, Illa Faradila Irmawati - -, Irmawati - Isnainar Isninar, Isnainar Jihan Jihan Jihan Jihan, Jihan Kasmudin Mustapa Lestari M.P. Alibasyah Lilies N Tangge Lilies Tangge Manap Trianto Maria Kanan Masriani . Masrianih Masrianih Masrianih Minarni Minarni Moh. Aziz Elfandi Mudrikah Tus Sa'diah Mulianti, Andi Mursito S. Bialangi Muslika Nifatun Umaya Nabila, Putri Ni Gusti Ketut Masmirayani Novi, Winda Cinthia Nuning Niwana Nuraini Nuraini Nurul Amna Moh Said Nurwahda, Nurwahda Pratiwi, Elly Qirey M Putri, Adelia Putu Widya Apriliani, Desak Rafiqa Rafiqa Rafiqa Rafiqah Rafiqah Rahayu, Ratna Dwi Rahmat Hidayat Raya Agni Rezkiana, Sry Ayu Rizal Wahid Permana Putra Safani Safani Sinta Sinta Sri Wahyuni Sri Wahyuni Suardi, Magfira Sudarsa, Caca Sukmawati Sukmawati Surahmin Syech Zainal Syech Zainal Tumale, Serli uunnadianti uunnadianti Vadillah Vadillah Vera Diana Towidjojo Windarsih, Yulia Zasmita, Eka Zulaikhah Dwi Jayanti