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All Journal Jambi Medical Journal : Jurnal Kedokteran dan Kesehatan Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Althea Medical Journal Molecular and Cellular Biomedical Sciences (MCBS) IRJE (Indonesian Research Journal in Education) Journal of Health Sciences MEDIC MEdical Dedication Jurnal Karya Abdi Masyarakat Journal of Medical Studies Indonesian Journal of Biomedicine and Clinical Sciences Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran

Anggelia Puspasari

Fakultas Kedokteran Dan Ilmu Kesehatan Universitas Jambi

Author-ID : 560413

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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GAMBARAN KADAR VITAMIN D PADA WANITA USIA SUBUR DI KOTA JAMBI; UPAYA AWAL PENGEMBANGAN MODEL KEBIJAKAN KESEHATAN REPRODUKSI Herlambang Herlambang; Nyimas Natasha Ayu Safira; Amelia Dwi Fitri; Anggelia Puspasari
Medical Dedication (medic) : Jurnal Pengabdian kepada Masyarakat FKIK UNJA Vol. 4 No. 2 (2021): MEDIC. Medical dedication
Publisher : Universitas Jambi

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ABSTRACT Deficiency and insufficiency of Vitamin D associated with reproductive associated illness in women. Previous studies reported increasing prevalence of preeclampsia, premature labor, intrauterine growth restriction and infertility in women who have deficiency and insufficiency of Vitamin D. Vitamin D plasma measurement does not perform as routine test in Indonesia, including Jambi province. Although Indonesia is tropical country that have glimpse of sunshine all year around but epidemiology study reported high prevalence of Vitamin D deficiency and insufficiency. Furthermore, we conduct Vitamin D screening in women in reproductive age as early step for develop policy associated with reproductive health. This program recruited 24 reproductive age women that getting explanation about objective and procedure will be performed. Demographic data and blood vein sample for Vitamin D measurement was taken. The author collaborated with Mitra Hospital and PRODIA laboratories for this program. Result of this screening showed all the participants have deficient and insufficient of Vitamin D plasma level, no one has normal Vitamin D plasma level. According to that result we suggested screening Vitamin D performed as routine screening for women in reproductive age and assessment of this caused of this phenomenon. Keyword: Vitamin D, Reproductive age women, reproductive health. ABSTRAK Kadar Vitamin D dibawah normal dikaitkan dengan gangguan kesehatan reproduksi pada wanita usia subur (WUS). Studi terdahulu menunjukkan peningkatan prevalensi preeklampsia, kelahiran prematur, pertumbuhan janin terhambat dan infertilitas pada WUS dengan insufisiensi dan defisiensi Vitamin D. Kadar Vitamin D bukan merupakan skrining rutin yang dikerjakan dilayanan kesehatan di Indonesia termasuk di Kota Jambi. Studi epidemiologi terdahulu menunjukkan meskipun Indonesia merupakan negara dengan sinar matahari sepanjang tahun, akan tetapi prevalensi defisiensi Vitamin masih tinggi. Sehingga pengabdian ini melakukan pemeriksaan kadar Vitamin D pada WUS sebagai upaya awal pengembangan model kebijakan kesehatan reproduksi. Sebanyak 24 orang WUS yang telah mendapatkan penjelasan tentang rangkaian kegiatan pengabdian setuju untuk mengikuti pengabdian ini. Data demografi dasar dan sampel darah vena untuk pemeriksaan kadar Vitamin D diambil pada kegiatan ini. Tim bekerjasama dengan RS Mitra dan laboratorium PRODIA terkait dengan pelaksanaan kegiatan dimasa COVID-19. Hasil pemeriksaan Vitamin D didapatkan kadar Vitamin D plasma dalam kategori insufisien dan defisien, tidak ada yang memiliki kadar plasma Vitamin D normal. Terkait hasil tersebut ,hendaknya skrining Vitamin D dapat dijadikan program pelayanan kesehatan rutin dan dilaksanakan kajian menyeluruh menegnai hal tersebut untuk mendukung intervensi lebih lanjut. Katakunci: Vitamin D, Wanita Usia Subur, Kesehatan Reproduksi

Genetic Variant of Vascular Endothelial Growth Factor (VEGF)-A rs699947 is Associated with Preeclampsia Anggelia Puspasari; Rina Nofri Enis; Herlambang Herlambang
Molecular and Cellular Biomedical Sciences Vol 6, No 2 (2022)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v6i2.241

Background: Preeclampsia remains as the leading cause of maternal-neonatal mortality and morbidity worldwide. Vascular endothelial growth factor A (VEGF-A) is a proangiogenic factor related to endothelial dysfunction and plays an important role in the preeclampsia pathophysiology. Genetic variants of VEGF-A are associated with VEGF-A expression and preeclampsia risk, however there are still inconsistent results between different populations. The aim of this study was to determine the association of this genetic variant as preeclampsia risk factor.Materials and methods: A cross-sectional study was performed with 76 pregnant women (29 preeclampsia and 47 normotensive) Jambi-Malay ethnic subjects. Sample DNA was extracted from subject’s blood. To determine the genotype, one-step tetra amplification refractory mutation system (ARMS) polymerase chain reaction (PCR) method for VEGF-A rs699947 C/A was used.Results: We found that pregnant woman with AC genotype (p-value=0.045; OR=2.76 ; 95% CI=1.01-7.58) and AA genotype (p-value=0.026; OR=12.44; 95% CI=1.23-126.18) had higher risk of preeclampsia than the CC genotype.Conclusion: Genetic variant VEGF-A rs699947 C/A is associated with preeclampsia. The AC and AA genotype is the risk genotype for preeclampsia in Jambi-Malay ethnics.Keywords: preeclampsia, VEGF-A, genetic variant, Jambi-Malay, Indonesia

The Role of Angiotensinogen rs699 in Diabetic Nephropathy Among Type 2 Diabetes Mellitus Patients with Uncontrolled Postprandial Glucose Levels Anggelia Puspasari; Devy Afriyanti; Huntari Harahap; Citra Maharani; Elfiani Elfiani
Jurnal Ilmiah Kesehatan Vol 15 No 03 (2022): Jurnal Ilmiah Kesehatan (Journal of Health Science)
Publisher : Universitas Nahdlatul Ulama Surabaya

Diabetic Nephropathy (DN) is the most common complication of Type 2 Diabetes Mellitus (T2DM), leading to the highest mortality rate of DM complications. However, its etiology is still questionable. Hyperglycemia, hypertension, and particular genetic susceptibility are associated with DN. Not all patients with uncontrolled hyperglycemia suffer DN. Thus, genetic susceptibility may be a risk factor for DN. The genetic variant of angiotensinogen rs699 is known to be associated with the risk of DN with inconsistent results between ethnicities. This study aims to reveal the correlation between the AGT rs699 with the incidence of diabetic nephropathy among type-2 diabetes mellitus patients with uncontrolled postprandial glucose levels in the Jambi Malay ethnicity. This study was observational analytic research with a cross-sectional design. It used 48 DNA samples from type-2 diabetes mellitus patients with uncontrolled postprandial glucose levels. The authors took 24 DNA samples from patients with DN and 24 without DN (as a control group). The genotyping method used ARMS-PCR specific for AGT rs699. Subjects with the CT genotype had a lower risk for diabetic nephropathy than the CC genotype, but it was not statistically significant (p=0.247; OR=0.508; 95%CI=0.160-1.607). In addition, subjects with the T allele (p=0.331; OR=0.621; 95%CI-0.237-1.630) had a lower risk for diabetic nephropathy than the C allele, but it was not statistically significant. In conclusion, Angiotensinogen rs699 is not a risk factor for diabetic nephropathy among type-2 diabetes mellitus patients with uncontrolled postprandial glucose levels in the Jambi Malay ethnicity.

Skrining Kadar Vitamin D Ibu Hamil di Wilayah Kerja Puskesmas Tahtul Yaman Kota Jambi Herlambang Herlambang; Nyimas Natasha Ayu Shafira; Citra Maharani; Rina Nofri Enis; Susan Tarawifa; Anggelia Puspasari; Erny Kusdiyah; Amelia Dwi Fitri
Medical Dedication (medic) : Jurnal Pengabdian kepada Masyarakat FKIK UNJA Vol. 5 No. 2 (2022): MEDIC. Medical dedication
Publisher : Universitas Jambi

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ABSTRACT Vitamin D is one of the nutritional components needed by the human body. This vitamin plays a role in maintaining healthy bones, teeth and muscles because it functions in terms of the absorption of calcium and phosphate. The need and function of vitamin D are also very influential on the condition of pregnancy for both mother and fetus because the compound 1,25 dihydroxy vitamin D (1,25(OH)2D) plays a role in the maintenance of cell function in the placenta. Another function of vitamin D is maintaining the mother's immune system properly. Fulfilment of vitamin D should be gradual from early pregnancy, and vitamin D deficiency causes pregnancy disorders and complications such as pre-eclampsia, premature birth, and stunted fetal growth. Examination of vitamin D levels is not included in routine pregnancy checks, so this service activity screens Vitamin D levels in pregnant women as an initial effort to prevent pregnancy disorders that can be triggered by low levels of vitamin D. A total of 24 pregnant women have received explanations and are willing to follow this activity. The service team collaborates with the Tahtul Yaman Health Center and also the PRODIA Laboratory in helping with the implementation of this service. The results of the Vitamin D examination showed that plasma Vitamin D levels were more in the insufficiency and deficiency categories, and only a small proportion had normal Vitamin D plasma levels. Based on these results, vitamin D screening should be included as an antenatal care program, and a thorough follow-up study should be carried out hereafter. Keywords: Antenatal care, Pregnancy, Vitamin D ABSTRAK Vitamin D merupakan salah satu komponen nutrisi yang dibutuhkan oleh tubuh manusia. Vitamin ini berperan dalam menjaga kesehatan tulang, gigi dan juga otot karena memiliki fungsi dalam hal penyerapan kalsium dan fosfat. Kebutuhan dan fungsi dari vitamin D ini juga sangat berpengaruh pada kondisi kehamilan baik untuk ibu maupun janin, karena senyawa 1,25 dihidroksi vitamin D (1,25(OH)2D) berperan untuk pemeliharaan fungsi sel pada plasenta. Fungsi lain dari vitamin D adalah untuk menjaga sistem kekebalan tubuh ibu agar tetap melakukan fungsinya dengan baik. Pemenuhan vitamin D sebaiknya bertahap dari awal kehamilan, defisiensi Vitamin D berdampak pada timbulnya komplikasi kehamilan seperti preeklampsia, persalinan prematur, dan pertumbuhan janin terhambat. Pemeriksaan kadar vitamin D tidak termasuk dalam pemeriksaan rutin kehamilan, sehingga kegiatan pengabdian ini melakukan skrining kadar Vitamin D pada ibu hamil sebagai upaya awal pencegahan timbulnya gangguan kehamilan yang dapat dipicu oleh rendahnya kadar vitamin D. Sebanyak 24 orang ibu hamil telah mendapatkan penjelasan dan bersedia untuk mengikuti kegiatan ini. Tim pengabdian berkerja sama dengan puskesmas Tahtul Yaman dan juga Laboratorium PRODIA dalam membantu terselanggaranya pengabdian ini. Hasil pemeriksaan Vitamin D didapatkan kadar Vitamin D plasma lebih banyak dalam kategori insufisien dan defisien, sebagian kecil yang memiliki kadar plasma Vitamin D normal. Berdasarkan hasil tersebut, sebaiknya skrining Vitamin D dapat dimasukkan sebagai program pemeriksaan rutin kehamilan dan dilakukan kajian lanjutan secara menyeluruh. Kata kunci: Kehamilan, Vitamin D, Pemeriksaan Kehamilan

Perbandingan Efektivitas Pembelajaran Clinical Skill Lab (CSL) dengan Menggunakan Video Ajar Keterampilan Klinik Neurologi terhadap Demonstrasi oleh Instruktur Aryanty, Nindya; Puspasari, Anggelia; Purwakanthi, Anati
JAMBI MEDICAL JOURNAL "Jurnal Kedokteran dan Kesehatan" Vol. 2 No. 2 (2014): JAMBI MEDICAL JOURNAL Jurnal Kedokteran dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

ABSTRACT Background : Medical Faculty University of Jambi has been implemented clinical skills lab (CSL) as one of learning methode. Limited time for encounter a learning topic which discuss just once in learning session may effect student ability to perform clinical skill of learning topic. There are many research represent advantages of learning video as effective learning media to gained student memory for a learning topic. The aim of this research was to compared effectiveness student clinical skill neurology examination ability using learning video with direct instructor teaching perform. Student ability to perform clinical skill assess using checklist procedure. Methode: This research was true experimental with posttest only control design. This research held at Medical Faculty University of Jambi in June until August 2014. Population of this research was all student in first years. Sample randomly selected and grouped as experiment (69 students) and control (68 students). Experiment group gave clinical skill neurology examination video, control group gave direct instructor teaching perform. Result: Evaluation based on checklist represent of experiment group had median value 78,13 (range 50,00) and control group had median value 74,25 (range 75,76). Mann U-Whitney test find the p- value for compared two group was 0,657 (p>0,05), there was no significant statistically between experiment and control group. Conclusion : In conclusion student who did not follow clinical skill learning session can learned from video, video may consider as learning media to over come limitation human resources and time of lecture in medical faculty. Kata Kunci : Clinical Skill Lab, Learning video, Neurology clinical skill

VARIASI GEN METHYLENETETRAHYDROFOLATE REDUCTASE PADA PREEKLAMPSIA; SEBUAH STUDI PENDAHULUAN PADA POPULASI JAMBI Maharani, Citra; Puspasari, Anggelia; Herlambang
JAMBI MEDICAL JOURNAL "Jurnal Kedokteran dan Kesehatan" Vol. 9 No. 1 (2021): Special Issues: JAMHESIC 2020
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

ABSTRACTBackground: Pre-eclampsia is a major cause of maternal and neonatal mortality and morbidity. The pathogenesismechanism of pre-eclampsia involves the interaction of genetic and environmental factors. Genetic variation in thegene MTHFR C677T (C> T transition) alter activity of MTHFR enzyme and predictrisk for pre-eclampsia but withconflicting results in worldwide population. This study aimed to investigate the association between gene variationof MTHFR C677T with pre- eclampsia in Jambi malay population.Method: This study was a case-control design. We compared the two subject groups; 30 pregnant women withpre-eclampsia and 30 normal pregnant women. Pre-eclampsia was diagnosed by elevated blood pressure (systolicbloodpressure ≥140 mmHg or diastolic blood pressure ≥90 mmHg) after 20 weeks of gestation.All samples wereethnic malay living in Jambi. The two groups were matched by age, gravida and parity. The Amplification RefractoryMutation System - Polymerase Chain Reaction was used for genotyping.Result and Conclusion: Association between the gene variation of MTHFR C667T and preeclampsia wasassessed using bivariate analysis with p<0,05. Statistical analysis of the additive model showed that the frequencyof TT genotype in thepre-eclamptic group was higher than normal pregnant women but did not show a significantdifference (OR = 0,833; 95% CI = 0,170 – 4,088; p= 0,568). Our study resultsuggest that the MTHFR C677T genevariation is not a risk factor for pre-eclampsia in theJambi malay population.Keywords: gene variation, Methylenetetrahydrofolate Reductase gene, MTHFR C677T, preeclampsiaABSTRAKPendahuluan: Mekanisme patogenesis preeklampsia melibatkan interaksi faktorgenetik dan lingkungan. Variasigenetik pada gen Methylenetetrahydrofolate Reductase (MTHFR) C677T (C>T transisition) menyebabkanpenurunan aktivitas enzim MTHFR yang berpengaruh pada peningkatan homosistein dan berkaitan dengankejadian preeklampsia. Penelitian ini bertujuan untuk mengetahui asosiasi antara variasi gen MTHFR C667Tdengan preeklampsia pada populasi melayu Jambi.Metode: Penelitian ini menggunakan rancangan kasus-kontrol. Kamimembandingkan dua subyek grup; 30 wanitahamil dengan preeklampsia dan 30 wanita hamil normal. Diagnosis preeklampsia ditentukan dengan adanyapeningkatan tekanan darah (tekanan darah sistolik ≥140 mmHg atau tekanan darah diastolik ≥90mmHg) setelah 20minggu kehamilan. Seluruh sampel merupakan etnis melayu yang berdomisili di provinsi Jambi. Kedua grup telahdicocokan berdasarkan usia ibu, gravida dan paritas. Pemeriksaan genotyping menggunakan metodeAmplification Refractory Mutation System - Polymerase Chain Reaction.Hasil: Asosiasiantara variasi gen MTHFR C677T dengan preeklampsia dinilai menggunakan analisis bivariatdengan p<0,05. Analisis statistik model additive menunjukkan frekuensi genotype TT pada kelompok hamil denganpreeklampsia lebih tinggi dibandingkan wanita hamil normal namun tidak menunjukkan perbedaan bermakna(OR=0,833; 95% CI=0,170 – 4,088; p=0,568).Kesimpulan: Hasil penelitian memberikan kesan variasi gen MTHFR C677T bukan sebagai faktor risiko terhadapkejadian preeklampsia pada populasi melayu Jambi.Kata kunci: variasi genetik, gen Methylenetetrahydrofolate Reductase, MTHFR C677T, preeklampsia

GAMBARAN NEUTROPHIL LYMPHOCYTE RATIO (NLR) PADA EARLY ONSET PREECLAMPSIA (EOP) DAN LATE ONSET PREECLAMPSIA (LOP) Nofrienis, Rina; Shafira, Nyimas Natasha Ayu; Herlambang, Herlambang; Elfiani, Elfiani; Puspasari, Anggelia; Fitri, Amelia Dwi; Kusdiyah, Erny; Harahap, Asro Hayani
JAMBI MEDICAL JOURNAL "Jurnal Kedokteran dan Kesehatan" Vol. 9 No. 3 (2021): JAMBI MEDICAL JOURNAL Jurnal Kedokteran Dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

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ABSTRACTBackground: Preeclampsia is a syndrome characterized by hypertension at gestational age above 20 weeks. Basedon the onset, preeclampsia is divided into EOP and LOP with a different time limit is 34 weeks gestation.Method: This study is a retrospective analytic using secondary data from medical record, the sampling method ispurposive sampling.Result: The results of the study, there were 175 cases of preeclampsia was obtained at Raden Mattaher Jambi Hospitalfrom the period 2014 - 2019. There were 31 patients with EOP and 146 patients with LOP, with the mean age of thestudy subjects 32.29 years for EOP and 31.68 years for LOP. The mean gestational age was 31 weeks for EOP andthe mean gestational age was 37 weeks for LOP. There were more multiparas for EOP and in LOP found morenulliparas and p value of 0.035. The median value of neutrophils was higher at EOP (10.20), while the value oflymphocytes was higher at LOP (2.00) but not statistically significant. The higher NLR frequency was found in the LOPgroup, which was 79.5% compared to the EOP group, which was 77.4% and not statistically significant.Conclusion: Descriptively, it was found that higher NLR values and higher NLR frequencies than the cut off point werefound in LOP, but they did not show statistically significant differences.Keywords: Preeclampsia, NLR, early onset, late onsetABSTRAKPendahuluan: Preeklampsia merupakan sindroma yang ditandai dengan hipertensi pada usia kehamilan diatas 20minggu. Berdasarkan onset, preeklampsia dibagi menjadi EOP dan LOP dengan batas waktu yang membedakanadalah 34 minggu gestasi.Metode: Penelitian ini merupakan analitik retrospektif dengan menggunakan data sekunder dari data rekam medis,metode pengambilan sampel adalah purposive sampling.Hasil: Dari hasil penelitian didapatkan sampel 175 kasus preeklampsia di RSUD Raden Mattaher Jambi dari periodetahun 2014 - tahun 2019. Terdapat 31 pasien dengan EOP dan 146 pasien dengan LOP dengan rerata usia subjekpenelitian 32.29 tahun untuk EOP dan 31.68 tahun untuk LOP. Untuk usia gestasi didapatkan rata-rata 31 mingguuntuk EOP dan rata-rata usia gestasi adalah 37 minggu untuk LOP. Dari status paritas, didaptkan lebih banyakmultipara untuk EOP dan pada LOP lebih banyak ditemukan nullipara dan nilai p 0,035. Nilai median neutrofil lebihtinggi pada EOP yaitu 10.20 sedangkan nilai limfosit lebih tinggi pada LOP yaitu 2.00 akan tetapi tidak berbedabermakna secara statistik.Kesimpulan: Frekuensi NLR yang lebih tinggi dijumpai pada kelompok LOP yaitu sebanyak 79.5% dibandingkan EOPyaitu 77.4% dan tidak bermakna secara statistik. Secara deskriptif didapatkan bahwa nilai NLR yang lebih tinggi danfrekuensi NLR yang lebih tinggi dari cut off point dijumpai pada LOP, akan tetapi tidak menunjukkan berbeda bermaknasecara statistik.Kata Kunci: Preeklampsia, NLR, early onset, late onse

EFEKTIVITAS ANTIBAKTERI EKSTRAK KULIT KULIT NANAS (Ananas Comosus) N-HEKSANA TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS ATCC 25923 Nadilla Sitepu; Ave Olivia Rahman; Anggelia Puspasari
Journal of Medical Studies Vol. 2 No. 1 (2022): Journal of Medical Studies
Publisher : Fakultas Kedokteran dan Ilmu Kesehatan Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/joms.v2i1.18093

ABSTRACT Background: Infectious diseases are generally treated using antibiotics and chloramphenicol is one of the main choice. However, it causes several side effects. Pineapple skin is thought to contain alkaloids, tannins, and terpenoids which are believed to have antibacterial activity. Objectives: This study aims to determine the effect of n-hexane exctract of pineapple peel on antibacterial activity of Staphylococcus aureus. Methods: In this study, we used fresh Pineapple skin that dried, powdered and macerated with n-hexane with ratio 1:10 ml extract were dried by rotary evaporator. A paper disc size 6 mm were soaked in the extract for 15 minutes, then ready to tested. Chloramphenicol as a positive control and n-hexane as a negative control. Antibacterial activity of extract against Staphylococcus aureus were tested by disk-diffussion method. Results: The results of the qualitative phytochemical test of pineapple peel extract of n-hexane showed positive for alkaloids and tannins, and negatif for terpenoid. There were no antibacterial activity of 100% n-hexane extract of pineapple skin against Staphylococcus aureus. While the positive control using chloramphenicol obtained an average clear zone diameter of 22.63 mm which is included in the very strong category. Conclusions: It concluded n-hexane extract of pineapple peel have no antibacterial activity against Staphylococcus aureus. Keywords: pineapple peels extract, Staphylococcus aureus, N-hexane ABSTRAK Latar Belakang: Penyakit infeksi umumnya diobati dengan antibiotik dan kloramfenikol merupakan salah satu pilihan utama. Namun, hal tersebut menyebabkan beberapa efek samping. Kulit nanas diduga mengandung alkaloid, tanin, dan terpenoid yang dipercaya memiliki aktivitas antibakteri. Tujuan: Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak n-heksana kulit nanas terhadap aktivitas antibakteri Staphylococcus aureus. Metode: Pada penelitian ini menggunakan kulit nanas segar yang dikeringkan, diserbukkan dan dimaserasi dengan n-heksana dengan perbandingan ekstrak 1:10 yang dikeringkan dengan rotary evaporator. Kertas cakram ukuran 6 mm direndam dalam ekstrak selama 15 menit, kemudian siap untuk diuji. Menggunakan kloramfenikol sebagai kontrol positif dan n-heksana sebagai kontrol negatif. Aktivitas antibakteri ekstrak terhadap Staphylococcus aureus diuji dengan metode difusi cakram. Hasil: Hasil uji fitokimia kualitatif ekstrak kulit nanas n-heksana menunjukkan positif alkaloid dan tanin, dan negatif untuk terpenoid. Tidak ada aktivitas antibakteri ekstrak n-heksana 100% kulit nanas terhadap Staphylococcus aureus. Sedangkan kontrol positif menggunakan kloramfenikol diperoleh rata-rata diameter zona bening sebesar 22,63 mm yang termasuk dalam kategori sangat kuat. Kesimpulan: Disimpulkan ekstrak n-heksana kulit nanas tidak memiliki aktivitas antibakteri terhadap Staphylococcus aureus. Kata kunci: Ekstrak kulit nanas, Staphylococcus aureus, N-heksana

THE VARIATION OF THE NOS3 GENE RS1799983 IS NOT ASSOCIATED WITH PREECLAMPSIA IN MALAY POPULATION Citra Maharani; Herlambang Herlambang; Anggelia Puspasari
Jambi Medical Journal : Jurnal Kedokteran dan Kesehatan Vol. 10 No. 3 (2022): Jambi Medical Journal: Jurnal Kedokteran dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

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ABSTRACT Background: eNOS play role in preeclampsia pathophysiology due to disturbing spiral arteries re-modelling and enhanced maternal vasoconstriction. A genetic variant of NOS3 associated with eNOS level. In addition, the previous study reported a genetic variant of NOS3 rs1799983 as a risk of preeclampsia with conflicting results beyond the population. To the best of our literature search, this genetic variant as preeclampsia risk has never been reported in the Jambi Malay population. This study aims to determine the association of the NOS3 genetic variant with preeclampsia in the Jambi Malay population. Methods: This study was a cross-sectional study that involved 43 pregnant women suffering preeclampsia and 53 normotensive pregnant women. All the subject resides in Jambi Province and was Jambi Malays. Genotyping was performed with tetra ARMS-PCR. Bivariate statistically analysis was performed. Result: The frequency of GT genotype was lower in the preeclamptic group and GG genotype was higher in the preeclamptic group than the TT genotype, but the difference was not statistically different for NOS3 rs1799983. Conclusion: Our findings indicate that the genetic variant of NOS3 rs1799983 is not associated with preeclampsia in the Jambi Malay population. Keywords: NOS3, eNOS, rs1799983, genetic variant, preeclampsia, Malay population ABSTRAK Pendahuluan: eNOS berperan dalam patofisiologi preeklamsia karena mengganggu remodelling arteri spiralis dan meningkatkan vasokonstriksi maternal. Varian gen NOS3 berkaitan engan peningkatan kadar eNOS. Penelitian sebelumnya melaporkan hasil yang bertentangan pada berbagai populasi terkait hubungan antara varian gen NOS3 rs1799983 dengan risiko kejadian preeklamsia. Sejauh pencarian literatur, varian gen NOS3 rs1799983 sebagai risiko preeklamsia belum pernah dilaporkan pada populasi Melayu Jambi. Penelitian ini bertujuan mengetahui hubungan varian gen NOS3 dengan preeklamsia pada populasi Melayu Jambi. Metode: Penelitian ini menggunakan desain potong lintang, melibatkan 43 orang ibu hamil dengan preeklamsia dan 53 orang ibu hamil normotensif. Semua subjek berdomisili di Provinsi Jambi dan merupakan orang Melayu Jambi. Pemeriksaan genotip dilakukan dengan metode tetra ARMS-PCR. Analisis statistik dilakukan meggunakan uji bivariat. Hasil: Hasil analisis didapatkan frekuensi genotip GT lebih rendah pada kelompok preeklampsia dan genotip GG lebih tinggi pada kelompok preeklampsia dibandingkan genotip TT, namun perbedaan tersebut tidak berbeda bermakna secara statistik. Kesimpulan: Hasil penelitian ini mengesankan bahwa varian genetik NOS3 rs1799983 tidak meningkatkan risiko preeklampsia pada populasi Melayu Jambi. Kata kunci: NOS3, eNOS, rs1799983, varian genetik, preeklamsia, populasi Melayu

A GENETIC VARIANT OF ANGIOTENSIN RS699 ASSOCIATED WITH DIABETIC NEPHROPATHY IN PATIENTS OLDER THAN 50 YEARS OLD Anggelia Puspasari; Elfiani Elfiani; Citra Maharani; Susan Tarawifa
Jambi Medical Journal : Jurnal Kedokteran dan Kesehatan Vol. 10 No. 3 (2022): Jambi Medical Journal: Jurnal Kedokteran dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

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ABSTRACT Background: Beyond controlling blood pressure and blood glucose, a genetic factor may play role in diabetic nephropathy (DN) progression in Type II Diabetes Mellitus (T2DM). Previous studies reported a genetic variant of Angiotensin rs699 (M235T) associated with diabetic nephropathy, with conflicting results across populations worldwide. To the best of our knowledge association of this genetic variant has never been conducted in the Jambi Malay population. Aims The Aims of this study is to the reveal association of the genetic variant of Angiotensin rs699 with diabetic nephropathy in the Jambi Malay population. Methods: This study design was cross-sectional. Totally 70 T2DM patients aged 22-67 years old were participated, as many 41 patients have aged older than 50 years old. Genotyping was performed using tetra ARMS PCR specific for rs699. Result: Patients aged older than 50 who have CTTT genotype have lower risk for suffering DN than CC genotype (p-value 0.029; OR (95% CI) 0.233 (0.06-0.88)), the statistic significant persist in multivariate analysis (p-value 0.030; OR (95% CI) 0.19 (0.04-0.86)). Conclusion: CTTT genotype was protective for DN in patients older than 50 years old. Further study with larger sample size, multi-centre and measuring confounding factors was needed. Keyword: Genetic variant of Angiotensin, rs699, diabetic nephropathy, Type II Diabetes Mellitus, Malay population ABSTRAK Latar belakang: Kendali Tekanan darah dan glukosa darah saja tidak cukup untuk mencegah kejadian nefropati diabetik (DN) pada Diabetes Mellitus Tipe II (T2DM), faktor genetik juga berperan penting. Studi sebelumnya melaporkan varian genetik Angiotensin rs699 (M235T) yang terkait dengan DN, dengan hasil yang berbeda antar populasi. Sejauh studi literatur yang dilakukan, varian genetik ini belum pernah diteliti pada populasi Melayu Jambi. Tujuan dari penelitian ini adalah untuk mengetahui hubungan varian genetik Angiotensin rs699 dengan nefropati diabetik pada populasi Melayu Jambi. Metode: penelitian ini merupakan penelitian potong lintang. Sebanyak 70 pasien T2DM berusia 22-67 tahun berpartisipasi, 41 diantaranya berusia lebih dari 50 tahun. Genotyping dilakukan dengan menggunakan tetra arms PCR spesifik untuk rs699. Hasil: Pasien berusia lebih dari 50 tahun yang memiliki genotipe CTTT memiliki risiko lebih rendah untuk menderita DN daripada genotipe CC (nilai p 0,029; OR (95% CI) 0,233 (0,06-0,88)), analisis multivariat juga menunjukkan hasil serupa (nilai p 0,030; ATAU (95% CI) 0,19 (0,04-0,86)). Kesimpulan: CTTT genotipe adalah pelindung untuk DN pada pasien yang lebih tua dari 50 tahun. Studi lebih lanjut dengan ukuran sampel yang lebih besar, berbagai pusat studi dan mengukur faktor risiko genetik dan non-genetik lain perlu dilakukan. Kata kunci: Varian genetik angiotensin, rs699, nefropati diabetik, Diabetes Mellitus Tipe II, populasi Melayu

Co-Authors Ahmad Hamim Sadewa Ahmad Syauqy Ahmad Syauqy Alya Dwiva Juhari Amalia, Fenny Amatullah, Afifah Amelia Dwi Fitri Amelia Dwi Fitri Amelia Dwi Fitri Amelia Dwi Fitri Amelia Dwi Fitri Amelia Dwi Fitri, Amelia Dwi Amelia Minarfah Anati Purwakanthi, Anati Annissa Delfira Arif, Cut Wulan Armaidi Darmawan Aryanti, Nindya Asro Hayani Harahap Ave Olivia Rahman Betty Marlina Budi Justitia Charles Apul Simanjuntak Citra Maharani Citra Maharani Devy Afriyanti dewi, hasna Dhea Assyifa Effendi, Ian Ekaputri, Tia Wida Elfiani Elfiani Elfiani Erny Kusdiyah Esa Indah Ayu, Esa Indah Fairuz, Fairuz Farrah Balqis Fitriyanti Gading, Patrick Wiliam Hana Nurul Qur’ani Harahap, Asro Hayani Hasmita, Debby Herlambang Herlambang HERLAMBANG HERLAMBANG Herlambang Herlambang Herlambang Herlambang Huntari Harahap Ika Setyawati Irawan Anasta Irlianissa, Mutiara Iskandar, Mirna Marhami Ismi Nurwaqiah Ibnu Khairunnisa, Dhia Fairuz Maharani, Amrina Mus, Rosdiana Nadilla Sitepu Neldi, Vina Nindya Aryanty Nurul Fitriani Nyimas Natasha Ayu Shafira Nys. Natasha Ayu S Pramudji Hastuti Priskila, Loury Rina Nofri Enis Rini Kartika Rita Halim Rita Halim S, Nys. Natasha Ayu Simanjuntak, Charles Apul Suhaimi, Novadian Suprapti Suprapti Susan Tarawifa Suzan, Raihanah Syatria Esa Putra Syukur, Astian Nathaniel Tarawifa, Susan Tarigan, Nadir Putra Indra Tengku Arief Buana Perkasa Tia Wida Ekaputri Utami, Erisca Ayu Virginsia, Sonya Vivekanantharasa, Raveenthiran Wahyu Indah Dewi Aurora Widyawati, Dwi Zulkhair Ali

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