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All Journal Medula JOURNAL OF HEALTHCARE TECHNOLOGY AND MEDICINE Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Jurnal Wiyata : Penelitian Sains dan Kesehatan Diklabio: Jurnal Pendidikan dan Pembelajaran Biologi Jurnal Teknologi Sistem Informasi dan Aplikasi PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Dunia Farmasi JURNAL ILMIAH OBSGIN : Jurnal Ilmiah Ilmu Kebidanan & Kandungan J-HESTECH (Journal Of Health Educational Science And Technology) PREPOTIF : Jurnal Kesehatan Masyarakat Journal of Herbal, Clinical and Pharmaceutical Science (HERCLIPS) Community Development Journal: Jurnal Pengabdian Masyarakat Medfarm: Jurnal Farmasi dan Kesehatan Jurnal Insan Farmasi Indonesia JOURNAL OF COMMUNITY ENGAGEMENT AND EMPOWERMENT Jurnal Farmasi Indonesia Jurnal Bisnis dan Ekonomi Media Farmasi JKEMS (Jurnal Kesehatan Masyarakat) Conference Proceedings International Conference on Education Innovation and Social Science International Journal of Contemporary Sciences (IJCS) Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan
Evi Kurniawati, Evi
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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Journal : Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan

 1 
VALIDASI METODE ANALISIS KUERSETIN DARI EKSTRAK ETANOL DAUN KEMANGI (Ocimum sanctum L) SECARA SPEKTROFOTOMETRI UV-Vis Kurniawati, Evi; Lestari, Tri Puji; Pertiwi, Krisna Kharisma
Jurnal Ilmiah Manuntung Vol 10 No 1 (2024): Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v10i1.780

Abstract

Basil leaves, scientifically known as Ocimum sanctum L., are a medicinal plant commonly used by the public because of their abundant secondary metabolite chemical compounds. One of them is flavonoids, secondary metabolites found in basil leaves, which have antioxidant properties. Quercetin, a flavonoid compound, exhibits valuable biological properties beyond its antioxidant effects, including antibacterial, anticancer, antiviral and anti-inflammatory activities. This research was carried out with the aim of determining the quercetin content contained in the ethanol extract of basil leaves (Ocimum sanctum L) by validated UV-Vis Spectrophotometry. Basil leaves are macerated with 70% ethanol. The extract is then concentrated using a rotary evaporator. Next, phytochemical screening was carried out to identify secondary metabolites in the extract, followed by UV-Vis spectrophotometric quantification. Based on the test results, it can be seen that the analysis of quercetin levels using UV-Vis spectrophotometry is validated for all parameters that meet the requirements. A significant relationship is shown by the linearity test, the correlation coefficient (r) is 0.9963. These results show good specificity, with test accuracy ranging from 90% to 107%, namely 103.9%. In addition, the precision test shows the %RSD value is less than 2%. The ethanol extract of basil leaves has an average quercetin content of 10.811% w/w.
Co-Authors Afandi Afriana, Afriana Ali Ikhwan Anis Akhwan Dhafin Ardianto, Dimas Arief, Muhtosim Aryani, Roza Asih Imulda Hari Purwani Dewy Resty Basuki, Dewy Resty Eko Sri Wahyuni Esti Ambar Widyaningrum Esti Ambar Widyaningrum Faisal Akhmal Muslikh Fendy Prasetyawan Fita Sari, Fita Fitriani, Marina Giska Tri Putri Herlinda Mawardika Ida Kristianingsih Intanri Kurniati Irawati, Renny Nur Satya Irawati, Reny Nur Satya Ismunanto, Aziza Regina Kinasih Jezmy, Beby Kelidia Kharisma, Krisna KRISNA KHARISMA PERTIWI Lestari, Tri Puji Ma'arif, Burhan Mawarni, Agrintya Indah Mita Riandini, Hanifa Muna, Sirajul Murhadi, T. Ninis Yuliati, Ninis Putri, Morica Angellia Shyama Rachma Nurhayati Rasmi Zakiah Oktarlina Ratna Dewi Puspita Sari Riadi, Refren Rilawati, Fentyana Dwi Rizky, Fara Nikhmatul Salmasfattah, Novyananda Shofiatul Fajriyah Shoviantari, M.Farm., Apt., Fenita Sony Andika Saputra Sugiyartono, Sugiyartono Susianti, Susianti Tri Puji Lestari Zenmas, Syendriva Zeptyan