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Firman Fajar Perdhana
Universitas Mataram
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Journal : Pro Food

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IDENTIFIKASI KANDUNGAN DAGING SAPI MENGGUNAKAN POLYMERASE CHAIN REACTION (PCR) PADA PEDAGANG BAKSO TUSUK KELILING DI LINGKUNGAN KOTA MATARAM, NUSA TENGGARA BARAT: Identification Of Beef Content Using PCR In Bakso Tusuk Roves Traders In The Mataram City Environment Lalu Unsunnidhal; Firman Fajar Perdhana; Qabul Dinanta Utama; Raudatul Jannah
Pro Food Vol. 9 No. 1 (2023): Pro Food (Jurnal Ilmu dan Teknologi Pangan )
Publisher : Fakultas Teknologi Pangan dan Agroindustri, Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/profood.v9i1.312

Abstract

Daging merupakan sumber protein penting dalam kehidupan sehari-hari manusia. Selain itu, dengan gaya hidup perkotaan yang sibuk dan padat, makanan olahan kini menjadi salah satu sumber protein utama dalam menu makanan seseorang. Konsumen bergantung pada pelabelan makanan untuk memutuskan apakah produk daging yang mereka beli aman dan terpercaya. Oleh karena itu, penting untuk memastikan pelabelan makanan dilakukan dengan cara yang benar untuk menghindari penipuan konsumen. Lebih banyak konsumen saat ini yang peduli dengan kualitas dan keamanan makanan daripada sebelumnya. Penelitian ini memaparkan metode untuk mengidentifikasi dan mendeteksi kandungan daging sapi menggunakan Polymerase Chain Reaction (PCR) pada 8 produk bakso tusuk dari pedagang keliling di sekitar Kota Mataram, Provinsi Nusa Tenggara Barat. Tujuan dari penelitian ini adalah untuk mengetahui kandungan daging sapi yang digunakan pada produk yang diuji. Temuan penting utama dalam karya ini adalah spesifisitas primer oligonukleotida untuk daging sapi olahan dan penentuan kandungan daging sapi pada bakso tusuk di sekitar Kota Mataram, Provinsi Nusa Tenggara Barat.
Optimasi Proses Ekstraksi Propolis dari Limbah Perasan Madu Trigona dengan Penggunaan Pelarut Air Tri Isti Rahayu; Sinaga, Yesica Marcelina Romauli; Perdhana, Firman Fajar; Zuhdia, Lulu Diani
Pro Food Vol. 10 No. 1 (2024): Pro Food (Jurnal Ilmu dan Teknologi Pangan)
Publisher : Fakultas Teknologi Pangan dan Agroindustri, Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/profood.v10i1.393

Abstract

Propolis is a natural resin produced by bees and has various health benefits that have been extensively studied. The extraction of propolis from Trigona honey pass residues generally uses organic solvent. Propolis can be extracted using water as a solvent with certain treatments. The study aims to obtain an optimal propolis extraction using a water solvent which is known to be safe and cheap. The optimization process of water extraction was combined with various extraction methods such as cold maceration, hot maceration (temperature 60°C), Ultrasound-Assisted Extraction (UAE), and Microwave-Assisted Extraction (MAE), compared to organic solvent controls and commercial propolis. The parameters observed from the obtained extracts are pH, phytochemical screening (alkaloids, phenolics, saponins, steroids, and flavonoids), color, antioxidant activity, and antibacterial activity. The data was analyzed using analysis of variance with the level of significance set at 5%. The results showed that the water extraction with MAE produced propolis extract with the highest antioxidant content and performed bacterial growth inhibition against Escherichia coli and Staphylococcus aureus.
Deteksi Salmonella typhi Berbasis Metode PCR pada Terasi Kering Padat Blok Produksi UMKM di Kabupaten Lombok Timur Ariyana, Mutia Devi; Unsunnidhal, Lalu; Perdhana, Firman Fajar; Febriandito, Muhammad ‘Aidil; Handayani, Baiq Rien; Zuhdia, Lulu Diani
Pro Food Vol. 11 No. 2 (2025): Pro Food (Jurnal Ilmu dan Teknologi Pangan)
Publisher : Fakultas Teknologi Pangan dan Agroindustri, Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/profood.v11i2.508

Abstract

Shrimp paste is a fermented shrimp product that is at risk of being contaminated by pathogenic microorganisms, thus potentially causing foodborne diseases. The method for detecting pathogenic microorganisms that has proven to have high specificity and sensitivity in directly detecting the presence of pathogenic bacteria in food samples is the DNA detection method using Polymerase Chain Reaction (PCR). This research aims to detect the presence of pathogenic bacteria, specifically Salmonella typhi, in shrimp paste products produced by several Small and Medium Enterprises (SMEs) in East Lombok Regency using the PCR method. The research samples used were solid block dried shrimp paste from three SMEs in East Lombok Regency. The research results show that the DNA of Salmonella typhi bacteria was not detected in all tested samples. These results indicate that the shrimp paste from the three SMEs in East Lombok has met one of the microbiological quality requirements for shrimp paste based on SNI 2716:2016, which is the absence of Salmonella contamination.
Co-Authors A Yuniastuti Adriani, Ika Reskiana Afni, Nur' Ahmad Alamsyah Amaliah, Wenny Amaliyah, Wenny Amaro, Moegiratul Amro, Moegiratul Anggraini, Ines Marisya Dwi Antesty, Sella Apriani, Tindih Ardo, Laela Aridho, Mulia Ariyanti, Baiq Haulia Baiq Rien Handayani, Baiq Rien Cicilia, Siska Devi Ariyana, Mutia Dilla Afriansyah Dody Handito, Dody Elya Antariksana Bachmida Febriandito, Muhammad ‘Aidil Gunawan, Rendi Hijayanti, Yatmi Idayanti, Fitri Inayati, Fitri Iqlima Khaerunnisa Kirtiyani, Ni Kadek Ayu Astiti Laily, Alfu Lalu Unsunnidhal Lestari, Risna Lingga Gita Dwikasari, Lingga Gita Lumbessy, Salnida Yuniarti Made Gendis Putri Pertiwi Made Gendis Putri Pertiwi Marisya Dwi Anggraini, Ines Mi’raj Fuadi Moegiratul Amaro Murad Mutia Devi Ariyana Mutia Devi Ariyana, Mutia Devi Namira, Afrisha Sekar Ningsi Ningsi NK Dewi Novia Rahayu Oki Saputra OKI SAPUTRA Pertiwi, Made Gendis Putri Putri Sulhana El Fiesha Balqis Putri, Mey Rizka R. Qabul Dinanta Utama Rasyda, Riezka Zuhriatika Ratih Dewanti -Hariyadi Raudatul Jannah Rini Nofrida Rizal Fahmi S., Ince Siti Wardatullatifah Sabilillah, Rizki Muhammad Ibnu Sella Antesty Setyaning Pawestri Sri Widyastuti Sukma, Dinda Galuh Tri Isti Rahayu Tri Isti Rahayu, Tri Isti Unsunnidhal, Lalu Vrisco Harjanto Wardatullatifah , Ince Siti Wati, Husnul Wati, Nila Susila Weriningsih, Wiharyani Wiharyani Werdiningsih Yarfaryh, Uswatun Hasanah Yasa, I Wayan Sweca Yeni Sulastri Yesica Marcelina Romauli Sinaga Zahra, Khoiruz Zalila, Nurfatin Zuhdia, Lalu Diani Zuhdia, Lulu Diani Zuhriatika Rasyda, Riezka