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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Fitopatologi Indonesia JURNAL SAINS PERTANIAN EQUATOR Perkebunan dan Lahan Tropika JURNAL ILMU PERTANIAN Agrovigor: Jurnal Agroekoteknologi Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Partner Jurnal Pertanian Agros Jurnal Agrotek Tropika Paspalum: Jurnal Ilmiah Pertanian Jurnal Agroplasma Planta Simbiosa : Jurnal Tanaman Pangan dan Hortikultura Variabel Agrifarm Jurnal Pengabdian Masyarakat dan Riset Pendidikan Sustainability Nexus: Journal of Agriculture
Fadjar Rianto
Universitas Tanjungpura
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Physics Social Sciences Veterinary Other

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Journal : Variabel

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Eksplorasi Bakteri Filoplan Daun Karet dan Potensinya sebagai Agens Hayati terhadap Pestalotiopsis sp. Penyebab Penyakit Gugur Daun Oktarianti, Sherly; Rianto, Fadjar; Syahputra, Edy
Variabel Vol 7, No 1 (2024): APRIL 2024
Publisher : STKIP Singkawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26737/var.v7i1.5309

Abstract

Karet di Indonesia merupakan salah satu peyumbang yang cukup besar terhadap devisa negara di sektor perkebunan. Upaya pengendalian digunakan untuk menekan penyakit gugur daun, Pengendalian hayati dengan memanfaatkan musuh alami diketahui dapat menekan pertumbuhan patogen. Diperlukan pencarian musuh alami yang efektif dalam mengendalikan patogen. Eksplorasi agens hayati merupakan langkah awal pada pengembangan pengendalian hayati. Penelitian ini bertujuan untuk eksplorasi bakteri filoplan daun karet yang berpotensi sebagai agens hayati terhadap Pestalotiopsis sp. penyebab penyakit gugur daun karet. Penelitian dilakukan di Laboratorium Penyakit Tanaman Fakultas Pertanian Universitas Tanjungpura Pontianak. Pelaksanaan penelitian dimulai dari eksplorasi bakteri filoplan pengambilan sampel bakteri filoplan tanaman karet, selanjutnya dilakukan isolasi dengan media Nutrient Broth, pemurnian dengan media Nutrient Agar dan identifikasi bakteri filoplan. Variabel yang diamati meliputi daya antagonisme bakteri filoplan terhadap Pestalotiopsis sp. dengan uji dual culture, uji reaksi gram dan respon hipersensitivitas (HR). Hasil eksplorasi mikroba filosfer pada daun karet ditemukan 6 isolat bakteri yang memiliki sifat antagonis dari 36 isolat yang ditemukan dilapangan yaitu FK10, FK11, FK21, FK24 dan FK26. Terdapat 4 isolat yang memiliki daya hambat dibawah 60 % yaitu FK2, FK10, FK11 dan FK24. Sedangkan 2 isolat memiliki daya hambat diatas 60% yaitu isolat FK21 dan FK26. Persentase penghambat tertinggi diperlihatkan isolat FK26 yaitu 61,2%. Isolat FK2, FK21, FK24 dan FK26 tidak bersifat patogen bagi tanaman pada hasil uji HR.Kata Kunci: Agens Pengendali Hayati; Bakteri Filoplan; Pestalotiopsis sp.; Tanaman KaretExploration of Rubber Leaf Filoplan Bacteria and Its Potential as a Biological Agent against Pestalotiopsis sp. Causes of Leaf Loss DiseaseABSTRACTRubber in Indonesia is a fairly large contributor to the country's foreign exchange in the plantation sector. Control efforts are used to suppress leaf fall disease. Biological control using natural enemies is known to suppress the growth of pathogens. It is necessary to search for natural enemies that are effective in controlling pathogens. Exploration of biological agents is the first step in the development of biological control. This research aims to explore rubber leaf phylloplan bacteria which have potential as biological agents against Pestalotiopsis sp. causes of rubber leaf fall disease. The research was conducted at the Plant Disease Laboratory, Faculty of Agriculture, Tanjungpura University, Pontianak. The research began with exploration of phylloplan bacteria, taking samples of phylloplan bacteria from rubber plants, then isolation using Nutrient Broth media, purification using Nutrient Agar media and identification of phylloplan bacteria. The variables observed included the antagonism of phylloplan bacteria against Pestalotiopsis sp. with dual culture test, gram reaction test and hypersensitivity response (HR). The results of exploration of phyllosphere microbes on rubber leaves found 6 bacterial isolates that had antagonistic properties from 36 isolates found in the field, namely FK10, FK11, FK21, FK24 and FK26. There were 4 isolates that have an inhibitory power below 60%, namely FK2, FK10, FK11 and FK24. Meanwhile, 2 isolates had an inhibitory power above 60%, namely isolates FK21 and FK26. The highest percentage of inhibitors was shown by isolate FK26, namely 61.2%. Isolates FK2, FK21, FK24 and FK26 were not pathogenic for plants in the HR test results.
Co-Authors ., Darussalam ., Osini agus ruliyansyah Ahmad Sulaiman Andika Lesmana Apriyani, Wenny ARIANTI KUNTARI Asmani, Asmani Bariyanto, Muhammad Deva Bella Sari, Natalia Wilhelmina Budi Fitriani Chatarina Umbul Wahyuni della nuaristi Dini Anggorowati Dwi Zulfita EDI NOFIARDI Edy Syahputra Eka Afriyan Hardigaluh Eni Handayani Erika Kornelia Sari Erinda, Novia Ety Herawati Fransiska Mada Sela Hana Septi Stella Rina Hearnes Napitupulu Hendro Muntarjo Heriandi, Heriandi Herly Annisa Herni Susanti Husnul Hakim Ikawati, Ratna Ikayanti, Fitri Iman Suswanto indri hendarti Indri Hendarti Indrian Tika Saputri irwan anshari Iwan Sasli Kingkun Karismando Kurnia, Lulu Mahmudi Marisa Nopriyanti Meita, Priskila Muhammad Faizal Mutiara - Neti, Neti Netty Indah Lestari Ningrum, Ajeng Maula Nisa, Yaumin Nizari Muhtarom NOFIARDI, EDI Norma Norma nortin . Nugroho, Muhammad Fikri Oktarianti, Sherly OKTAVIANI, OKA Pivi Sopiany Radian Radian Ramadhan, Dicky Ramanda, Rika Fitry RESTU NURKHAMDANI Ridwan Ridwan Riki Warman Rumambi, Ellia Septiarahma Safriadi Salsabila, Unik Hanifah Sarbino Sarbino SARBINO SARBINO Sarbino Sarbino Sarbino Sarbino Sarbino Sarbino Sari, Natalia Wilhelmina Bella selamet aswawi Senangka, Vinsensius Bagus Siti Raihany Luthfiyyah SOFIAN, NANA SRI RAHAYU Subiantoro, Subiantoro Supriyanto Supriyanto Syafiuddin, Wasi’an Tantri Palupi Tatang Abdurrahman, Tatang Toni, Toni TRIS HARIS RAMADHAN Tris Haris Ramadhan Valentino, Fernando Wahyuni, Elsa Warman, Riki WASI'AN WASI'AN WASI'AN WASI'AN Wasi'an Wasi'an Wasi'an, Wasi’an Wasian - wasian wasian, wasian Wasi’an, Wasi’an Yaskur, Yaskur Yuaner, Yuaner Zailani, Indra Wahyudi Zakiatulyaqin, -