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All Journal International Journal of Public Health Science (IJPHS) Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Pengabdian Magister Pendidikan IPA Jurnal Kedokteran Universitas Palangka Raya Jurnal Pepadu Promotor: Jurnal Mahasiswa Kesehatan Masyarakat
Ayunda, Rahmah Dara
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Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Journal : Jurnal Kedokteran Universitas Palangka Raya

 1 
Isolasi bakteri asam laktat asal feses bayi dan potensinya dalam menghambat pertumbuhan Escherichia coli Hasbi, Nurmi; Rosyunita; Rahim, Adelia Riezka; Ayunda, Rahmah Dara; Parwata, Wayan Sulaksmana Sandhi; Farras, Afif; Raihan, Al Fikar; Billah, Muhammad Azim
Jurnal Kedokteran Universitas Palangka Raya Vol 12 No 1 (2024): Jurnal Kedokteran Universitas Palangka Raya
Publisher : Fakultas Kedokteran, Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37304/jkupr.v12i1.12852

Abstract

Lactic acid bacteria (LAB) play an important role in improving performance and mitigating disease because of their ability to maintain the physiological balance of the gastrointestinal tract and resistance to pathogenic bacteria. LAB is one of the bacteria found in the digestive tract. LAB must be isolated from their hosts to increase their efficiency as probiotic ingredients. This study aims to obtain local isolates of LAB a from baby feces through phenotypic identification and antibacterial test. The sample used in the research was the feces of babies aged 1-6 months that patients from Mataram University Hospital. Isolation was carried out by growing bacteria from feces using the Total Plate Count method on MRSA media plus CaCO3. Phenotypic characterization is morphology test, gram stain and biochemical tests. The results of bacterial isolation obtained 8 isolates of LAB. LAB produced a clear zone in the MRSA media, because these bacteria were able to secrete acid in the MRSA. The results of gram staining show that all isolates are gram positive bacteria. Based on the morphology of LAB had characteristic 6 isolates of cocci and 2 isolates of bacilli. All isolates was able to produce an inhibition zone against E. coli with 3 isolates in the moderate category and 5 isolates in the weak category. The best inhibition zone in the medium category is for cocci-shaped bacteria.The existence of LAB isolates from baby feces can be used as a reference source for further research on probiotics such as other antibacterial test
Isolasi Staphylococcus aureus dari Swab Tangan Penjamah Makanan di Kantin Universitas Mataram Hasbi, Nurmi; Rosyunita; Rahim, Adelia Riezka; Ayunda, Rahmah Dara
Jurnal Kedokteran Universitas Palangka Raya Vol 12 No 2 (2024): Jurnal Kedokteran Universitas Palangka Raya
Publisher : Fakultas Kedokteran, Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37304/jkupr.v12i2.15313

Abstract

One of the main qualities of food is seen from the biological safety aspect, such as being free from Pathogenic microorganisms. Staphylococcus aureus is a pathogenic bacterium that can contaminate food ingredients come from the skin of food handlers. S. aureus produces enterotoxins that have a poisoning in consumers.The preliminary observationstated that there were still many food handlers in the Mataram University canteen who did not pay attention to hand hygiene and personal sanitation. This study aims to determine the bacterial contamination of S. aureus swabbed from the hands of food handlers in the Mataram University cafetaria. The method was used in this research is descriptive observational. The research consisted of two stages, namely isolation and biochemical identification. The isolation stage resulted 10 culturable bacterial isolates. All bacteria were able to ferment mannitol on Mannitol Salt Then, the biochemical test identification resulted that all isolates had a positive catalase test, a positive slide coagulase test, Gram-positive, and staphylococci cell. The TSIA test resulted A/A or (acid)/(acid) which indicated that all isolates were able to use diverse carbon sources from sucrose, lactose, glucose. Moreover, other test as sulfur, indole and motility tests were negative. Therefore, all isolates in this study are classified as S. aureus. This research is also a promotive and preventive effort for the campus in implementing hygiene and sanitation for food handlers in the cafetaria.
Co-Authors Abdiman, I Made Tobias Amaliya, Annisa Risqi Andrayani, Lale Wisnu Ardiana Ekawanti Ario Danianto, Ario Arnawati, Ida Ayu As’ad, Suryani Azizatul Adni Balqis, Ananda Amirah Billah, Muhammad Azim Citranggana Prajnya Dewi Deasy Irawati Elizar, Lale Justin Amelinda Farras, Afif Gama, Noviyanty Indjar Hasbi, Nurmi - Ima Arum Lestarini Kadriyan, Hamsu Khairil Pahmi Lale Justin Amelinda Elizar Lazuardi Arsy, Lazuardi Lina Nurbaiti, Lina Muhammad Abdurrosyid Natsir, Rosdiana Parwata, Wayan Sulaksmana Sandhi Prasetyastuti, . Rahim, Adelia Riezka Rahmadhona, Devi Raihan, Al Fikar Rifana Cholidah, Rifana Rosyunita Rosyunita, Rosyunita Sesariana, Mayumi Agestia Shofa, Dhiya Atsila Steven Christian Suryantarini, Ni Wayan Puspa Wijaya Umar, Husaini Wiwin Lastyana Zubaidi, Fitriannisa Faradina