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Acetyl-CoA Carboxylase Alpha Gene Polymorphism and Its Association with Milk Fatty Acid of Holstein Friesian Using Real-Time PCR Method R. Azis; Jakaria; A. Anggraeni; A. Gunawan
Tropical Animal Science Journal Vol. 43 No. 4 (2020): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2020.43.4.306

Abstract

Milk fatty acids are largely affected by genetic factors. Acetyl-CoA Carboxylase Alpha (ACACA) gene is one of the important genes in regulation and metabolic function of milk fatty acids in dairy cattle. The objective of this study was to find out the relationship of single nucleotide polymorphism (SNP) ACACA gene with milk fatty acid trait in local dairy cattle. A total of 277 samples of Holstein Friesian (HF) were collected from Indonesian Research Institute for Animal Production (IRIAP), Animal Breeding Center and Forage Feed of Baturraden (ABCFFB), Central Java Province, Animal Husbandry Training Center of Cikole (AHTCC), West Java Province, Singosari Artificial Insemination Station (Singosari AIS), East Java Province, and Lembang Artificial Insemination (Lembang AIS), West Java Province, Indonesia. Genotyping of this SNP marker (g.2203G>T) was analyzed using the real-time Polymerase Chain Reaction (PCR) based on the hybridization TaqMan probe as the method for allelic discrimination. Milk samples were analyzed using Gas Chromatography and Mass Spectrometry (GCMS). The results of this study revealed the GG and GT genotypes. The proportion of the GG genotype frequency (0.88) was higher than the GT genotype (0.11) and the G Allele frequency was shown higher than the T allele in all locations, i.e., 0.942 and 0.08, respectively. The ACACA gene g.2203G>T SNP was significant (p<0.05) for lauric (C12:0) and dodecanoic (C17:1) acids. It was concluded that the ACACA gene g.2203G>T SNP could be useful as a marker selection for milk fatty acid such as lauric and dodecanoic fatty acids.
Pelatihan Managemen Budidaya Itik untuk Meningkatkan Produksitivas Kelompok Ternak di Desa Slorok Kecamatan Doko Kabupaten Blitar Rosidi Azis; Lestariningsih Lestariningsih
BRILIANT: Jurnal Riset dan Konseptual Vol 3, No 4 (2018): Volume 3 Nomor 4, November 2018
Publisher : Universitas Nahdlatul Ulama Blitar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (127.794 KB) | DOI: 10.28926/briliant.v3i4.236

Abstract

Budidaya itik di Desa Slorok Kecamatan Doko Kabupaten Blitar produktivitasnya rendah. Program pelaksanaan Pengabdian Kemitraan Masyarakat (PKM) ini bertujuan untuk meningkatkan produktivitas itik hibrida yang dipelihara oleh kelompok Peking dan Sido Makmur. Metode yang digunakan dalam pelaksanaan ini yaitu survey terhadap masalah, analisis masalah dan penentuan fokus masalah. Pelatihan budidaya itik meliputi managemen pakan, sanitasi dan kesehatan itik hibrida. Hasil dari pelaksanaan PKM yaitu managemen pakan dilaksanakan diberikan pukul 06.00 WIB dan pukul 15.00 WIB. Pakan yang diberikan harus memenuhi syarat gizi pakan. Managemen sanitasi dilaksanakan untuk meminimalisir terserangnya penyakit pada itik. Penyakit yang sering terjadi pada itik di keompok ini yaitu ND, AI, Snot, CRD dan DHV. Ternak yang terinfeksi penyakit pelru dipisah dikandang yang berbeda dengan ternak lainnya. Kesimpulan kegiatan PKM ini sebagai upaya untuk meningkatkan produktivitas melalui managemen pakan, sanitasi dan kesehatan ternak itik hibrida super.
Analisis pengaruh LED inframerah sebagai pengawet susu formula cair Muhammad Helmi Hakim; Ratika Sekar Ajeng Ananingtyas; Rosidi Azis
BRILIANT: Jurnal Riset dan Konseptual Vol 6, No 1 (2021): Volume 6 Nomor 1, Februari 2021
Publisher : Universitas Nahdlatul Ulama Blitar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.417 KB) | DOI: 10.28926/briliant.v6i1.590

Abstract

Penelitian ini dilakukan untuk menganalisis pengaruh LED inframerah sebagai potensi dalam pengawetan kualitas susu formula cair yang termasuk salah satu jenis pangan mudah rusak. Penelitian yang dilakukan adalah penelitian kuantitatif desain true experiment. Data yang diperoleh adalah data hasil uji TPC kelompok kontrol tanpa paparan 0 LED inframerah yang didiamkan selama 2,5 jam dan kelompok pemaparan dengan dipapari 15 LED inframerah juga selama 2,5 jam di tempatkan di atas botol susu formula cair. Hasil rata-rata nilai TPC untuk kelompok kontrol didapatkan 16,93 X 105 CFU/mL. Sedangkan rata-rata nilai TPC untuk kelompok pemaparan didapatkan 2,7 x 105 CFU/mL. Dari hasil analisis uji statistik inferensial One way-Anova post-hoc Tukey didapatkan nilai signifikansi sebesar 0,006 < 0.05. Sehingga terdapat pengaruh yang signifikan pada susu formula cair yang dipapari LED inframerah terhadap penurunan jumlah bakteri dari hasil uji TPC.
Association of Stearoyl-CoA Desaturase Gene Polymorphisms on Milk Fatty Acid Composition of Holstein Friesian Cows in Indonesia R. Azis; A. Anggraeni
Tropical Animal Science Journal Vol. 46 No. 1 (2023): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2023.46.1.27

Abstract

Milk fatty acid composition determines milk quality and the health of consumers. Stearoyl-CoA desaturase (SCD) gene controls lipid metabolism in the mammary gland, which plays an essential role in catalyzing saturated fatty acids (SFA) into monounsaturated fatty acids (MUFA). This study aimed to analyze the genetic diversity of the SCD gene and its effect on the milk fatty acid quality of Holstein Friesian (HF). Genetic variants were identified on 226 HF cattle from various dairy breeding Stations in West Java, Central Java, and East Java in Indonesia. Blood samples were extracted and amplified using Real-Time Polymerase Chain Reaction (RT-PCR) technique based on TaqMan Probe to determine the genotype of the SCD gene at g.10153A>G locus. Milk samples were analyzed by Gas Chromatography and Mass Spectrometry (GCMS) on 48 lactating cows. The SCD g.10153A>G produced three genotypes, namely AA (0.112), AG (0.640), and GG (0.271); and two alleles, namely A (0.409) and G (0.591). Genotype polymorphism of the SCD g.10153A>G had a significant effect (p<0.05) on SFA, including propionic acid (C3:0), butyric acid (C4:0), caprylic acid (C8:0), and pentacosylic acid (C25:0); and MUFA including hexadecenoic acid (C16:1) and total fatty acids. The SCD g.10153A>G SNP (AG genotype) could be one of the candidates as a genetic marker to assist selection in reducing SFA and increasing MUFA in the milk content of HF cows in Indonesia.
Phenotypic Similarities and Differences of Genetic Characteristic Boer and PE Goat Yield Crossbreeding 1st and 2nd Generation Rosidi Azis; Gatot Ciptadi; S. Suyadi
Chalaza Journal of Animal Husbandry Vol 5, No 2 (2020): Chalaza Journal of Animal Husbandry
Publisher : UNIVERSITAS SEMBILANBELAS NOVEMBER KOLAKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (968.76 KB) | DOI: 10.31327/chalaza.v5i2.1364

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This research aimed to analyze the phenotypic similarity and genetic characteristics of 1st and 2nd generation (G1 and G2) goat of Boer and PE goat yield crossbreeding. The number of offsprings of G1 and G2 was46 and 28 goats, respectively. Data analysis of phenotypic characteristics was estimated by calculating the opportunities of the number of the appearance of G1 and G2 offspring. The result showed that the percentages of the G2 of  W11 similarity (67.86%) more significant than G1 (26.09%).  The W21was the G1 (45.65%) higher than G2 (10.71%), and W31 was G1 (28.26%) higher than G2 (21.43%). It concluded that G2 had phenotypic similarities of the characteristic (color) in total progeny. The G2 goat displays the phenotypic characters of inheritance (color)patterns that had more significant similarity than the results of the G1, as well as similarities in morphological characters that were different from the G1. The characteristics (colors) in the G2 had dark brown head color greater than G1, and body conformation approaches to Boer goat. Other colors like light brown, plain white, black, and straight black on G1 greater than G2.
Application of Infrared with Different Waves and Its Effect on Organoleptic of Dairy Milk Rosidi Azis; Muhammad Helmi Hakim
Chalaza Journal of Animal Husbandry Vol 6, No 1 (2021): Chalaza Journal of Animal Husbandry
Publisher : UNIVERSITAS SEMBILANBELAS NOVEMBER KOLAKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (704.669 KB) | DOI: 10.31327/chalaza.v6i1.1385

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Infrared applications have been widely used to reduce microorganisms in a product and food preservation. Milk is a food product that quickly decomposes, where the changes can be observed organoleptically. This study aimed to test infrared with various wavelengths of milk and observe changes in the organoleptic of milk. The method used was that the dairy milk samples were treated with infrared (IR) exposure of 880 nm and 940 nm. Observation of the changes in milk organoleptic, namely color, taste, and smell. The effectiveness of IR 880 nm has a better ability than IR 940 nm for smell and taste, while for IR 940 nm, color is better than IR 880 nm at 1 - 4 hours, and at 5 hours with 940 nm IR it changes white-brownish.
Karakterisasi Protein Total Konsentrat Daun Kelor (Moringa oleifera Lam) Menggunakan Spektrofotometer UV-VIS Yuanita Amalia Hariyanto; Rosidi Azis; Yuniar Alam; Anindya Bella Monica
BRILIANT: Jurnal Riset dan Konseptual Vol 7 No 1 (2022): Volume 7 Nomor 1, Februari 2022
Publisher : Universitas Nahdlatul Ulama Blitar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (782.82 KB) | DOI: 10.28926/briliant.v7i1.884

Abstract

Salah satu tumbuhan yang keberadaannya sangat melimpah di Indonesia dengan berbagai macam manfaatnya adalah daun kelor (Moringa oleifera Lam). Daun Moringa oleifera memiliki potensi sebagai bahan feed additives karena tersebar luas di wilayah Indonesia. Di Indonesia para peternak ruminansia sering menggunakan daun Moringa oleifera sebagai imbuhan pakan karena dipercaya dapat menghasilkan daging yang berkualitas tinggi. Penelitian ini bertujuan untuk menganalisis kandungan protein total yang ada dalam daun Moringa oleifera dengan menggunakan metode Bradford. Ekstraksi daun Moringa oleifera lalu di keringkan dengan suhu 30 oC – 35 oC dan di ayak menggunakan ayakan 200 mesh untuk diperoleh serbuk nano. Selanjutnya, disintesis konsentrat protein dengan tambahan akuades dan larutan Bradford untuk di ukur dengan spektrometer pada panjang gelombang ( λ ) 595 nm. Berdasarkan pengukuran menggunakan spektrofometer UV-Vis diperoleh kadar protein total untuk pengujian simplo, duplo, dan triplo sebagai berikut 9,009%, 9,689%, dan 9,495%.
Pelatihan Pembelajaran Biologi Interaktif Untuk Guru Sekolah Pedesaan Muttaqin, Muhammad Zainul; Rahmawati, Sri; Rafid, Rahmad; Azis, Rosidi; Adi, Aprianus
Journal of Community Development Vol. 3 No. 3 (2023): April
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/comdev.v3i3.1487

Abstract

Interactive Biology learning training for rural school teachers aims to improve the quality of teaching by utilizing learning methods that actively involve students, despite the limited resources available. This study uses a qualitative approach with phenomenological design to explore the teacher's experience and perception of the training. Data were collected through in-depth interviews, classroom observations, and documentation. The results showed that this training has a positive impact on improving the knowledge and skills of teachers in implementing interactive Biology learning. Teachers feel more confident in using simple teaching aids and environment-based experiments as a learning medium. However, the main challenge faced is the limited facilities and teaching aids in rural schools. In addition, despite the increase in student activity, teachers also face difficulties in managing the diversity of student interests and abilities. Overall, the training improves the quality of teaching in rural schools and provides practical guidance to address existing challenges. This research suggests that continuous training should be conducted and focus on practical solutions related to limited resources
Pemanfaat Limbah Industri Furniture Sebagai Produk Kreatif Yang Memiliki Nilai Tambah Ekonomi Untuk Karang Taruna di Desa Panggung Duwet Blitar Azis, Rosidi; Ali, Moh.; Wahyuni, Sri; Priyanti, Novie Ary
Journal of Community Development Vol. 3 No. 3 (2023): April
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/comdev.v3i3.1489

Abstract

Utilization of furniture industry waste into creative products with added economic value is one of the efforts to improve community welfare through innovation based on local resources. This study focuses on the processing of wood waste from the furniture industry in Panggung Duwet Village, Blitar, into flower pots that have attractive designs and are in accordance with modern home decoration trends, especially those known as instagrammable. The processing process begins with sorting and cutting wood waste, followed by smoothing the surface using sandpaper, and ending with a finishing that enhances the aesthetics of the product. Innovation in flower pot design with a minimalist and natural appearance utilizing wood materials combined with natural elements, provides high appeal in the market. This study also discusses the fulfillment of production facilities in wood workshops, which include the provision of cutting, smoothing, assembly, and finishing tools, which support the efficiency of the production process. The results of the study indicate that flower pot products have great market potential, especially among ornamental plant lovers and consumers who are interested in environmentally friendly and unique home decoration products. In addition to the positive economic impact on Karang Taruna members, this program also contributes to empowering village communities through waste processing skills into high-value products. Overall, processing furniture waste into flower pots not only supports environmental sustainability but also creates new economic opportunities for village communities.
Perspektif Inovasi dan Strategi Pembelajaran Biologi di Era Revolusi Pendidikan Abad 21 Priyanti, Novie Ary; Habiburrahman, Novan; Dwi Andrian, Risnanda i; M. Amaral, Lucia; Selan, Sarlotha; Azis, Rosidi
Jurnal Pembelajaran dan Riset Pendidikan Vol 3 No 4 (2023): Volume 3, No. 4, Oktober 2023
Publisher : Lembaga Penelitian dan Pengabdian Universitas Nahdlatul Ulama Blitar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28926/jprp.v3i4.1716

Abstract

Pembelajaran biologi di abad ke-21 mengalami transformasi signifikan dengan mengintegrasikan teknologi digital dan pendekatan pedagogis inovatif untuk membekali siswa dengan keterampilan abad ke-21, seperti berpikir kritis, kolaborasi, komunikasi, dan kreativitas. Pendekatan berbasis inkuiri, pembelajaran berbasis proyek, serta integrasi lintas disiplin menjadi strategi utama untuk meningkatkan relevansi materi biologi dengan tantangan dunia nyata. Teknologi seperti laboratorium virtual dan platform pembelajaran daring memungkinkan siswa memperoleh pengalaman belajar yang interaktif dan global. Namun, tantangan seperti kesenjangan akses teknologi, kesiapan guru, serta keterbatasan sumber daya menjadi hambatan dalam penerapan pembelajaran modern. Strategi inklusif yang mencakup penyediaan fasilitas, pelatihan guru, dan integrasi konten lokal menjadi solusi untuk mengatasi hambatan tersebut. Pendekatan yang adaptif dan kolaboratif mampu mencetak generasi yang tidak hanya memahami ilmu biologi secara mendalam tetapi juga mampu memberikan solusi terhadap permasalahan global seperti perubahan iklim dan keberlanjutan ekosistem. Artikel ini menyoroti peluang, tantangan, dan strategi untuk meningkatkan kualitas pembelajaran biologi yang relevan dan berkelanjutan di era modern.