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Anggreini, Putri
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Phytochemical Screening, Antioxidant Activity, And Antimitotic Potential Of Shallot Skin (Allium Cepa L.) Extract Fadilla, Raisa; Arief, Maryam Jamila; Badawi, Satriani; Sinthary, Venna; Anggreini, Putri; Wahyuni, Leny Eka Tyas; Nisa, Nur Rezky Khairun
Journal of Innovative and Creativity Vol. 6 No. 1 (2026)
Publisher : Fakultas Ilmu Pendidikan Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/joecy.v6i1.6915

Abstract

Shallot skin (Allium cepa L.) is a by-product that is generally discarded as household waste, despite its potential as a source of bioactive compounds. Previous studies have indicated that shallot skin contains secondary metabolites with antioxidant and cytotoxic properties. This study aimed to evaluate the phytochemical composition, antioxidant activity, and antimitotic potential of shallot skin extract in order to support its valorization as a natural bioactive resource. Materials and methods. This research was conducted as an experimental laboratory study. Dried shallot skin was extracted using ethanol through a maceration method, followed by solvent evaporation to obtain a crude extract. Phytochemical screening was performed using Thin Layer Chromatography (TLC) to identify major secondary metabolites. Solvent partitioning was carried out using n-hexane and ethyl acetate, and further fractionation was conducted using vacuum column chromatography and preparative TLC. Antioxidant activity was evaluated using the DPPH radical scavenging assay, while antimitotic activity was assessed using the Brine Shrimp Lethality Test (BSLT). Results. The ethanolic extraction produced a high yield of crude extract (57.13%), indicating effective recovery of polar and semi-polar compounds. Phytochemical screening confirmed the presence of alkaloids, flavonoids, and phenolic compounds. The extract exhibited very strong antioxidant activity with an IC₅₀ value of 17.477 µg/mL. Antimitotic evaluation using BSLT showed an LC₅₀ value of 202.301 µg/mL, indicating biological activity at moderate concentrations. Conclusions. The findings demonstrate that shallot skin contains diverse secondary metabolites with strong antioxidant activity and measurable antimitotic potential. These results support the utilization of shallot skin as a valuable natural source of bioactive compounds and provide a basis for further studies on compound isolation and advanced biological evaluation.
Co-Authors Akhmad Akhmad Andi Tenri Kawareng Annisa, Oktavia Nasrul Arief, Maryam Jamila Aulia, Nasywa Badawi, Satriani Danri Toni Siboro Dwiyono, Yudo Fadilla, Raisa Fahrul Rozi Febriyanthy, Adhiesty Syaffila Haeruddin Haeruddin Herman Herman Ibrahim, Arsyik Indriyani, Feby Irfansyah Baharuddin Pakki Junaidin, Junaidin Laode Rijai Lizma Febrina LUMBAN GAOL, MANATAP BERLIANA Majiding, Chaidir Masyhuri Muhammad Nuzul Azhim Ash Siddiq Nisa, Nur Rezky Khairun Nisaa, Nur Rezky Khairun Olivia Siregar, Vita Pijaryani, Indria Putra, Arya Yuliansyah Rahmadani, Aulia Tia Rashif Rijai, Hifdzur Rezky Khairun Nisaa, Nur Rijai, Hifdzur Rashif Sapitri, Salsabila Dwi Sari, Mila Warma Siddiq, Muhammad Nuzul Azhim Ash Sinthary, Venna Siregar, Vita Olivia Syaifur Rochman Ummi Khuzaimah Wahyuni, Leny Eka Tyas Wirawanti, Ika Wirya Yuliani, Shahibah