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EFEKTIVITAS PENAMBAHAN SERUM SAPI PESISIR FASE BERAHI TERHADAP PEMATANGAN OOSIT KERBAU SECARA IN VITRO Jhon Hendri; Haris Satria; Alfian Asri; Jaswandi Jaswandi
Jurnal Peternakan Vol 15, No 2 (2018): September 2018
Publisher : State Islamic University of Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/jupet.v15i2.5636

Abstract

Penelitian ini bertujuan untuk mengetahui persentase oosit kerbau yang matang dalam media TCM-199 yang disuplementasi serum sapi pesisir fase berahi secara in vitro. Selanjutnya untuk meningkatkan efisiensi produksi embrio in vitro pada ternak kerbau dengan suplementasi serum sapi pesisir fase berahi. Oosit ternak kerbau dimatangkan dalam media TCM-199 pada inkubator Co2 5% dan masing-masing perlakuan ditambahkan serum sapi pesisir fase berahi dengan konsentrasi yang berbeda (0%, 10% dan 20%). Peubah yang diamati dalam penelitian ini adalah persentase oosit kerbau yang matang tanpa penambahan serum sapi pesisir fase berahi, persentase oosit yang matang dengan penambahan serum sapi pesisir fase berahi 10 % dan persentase oosit kerbau yang matang dengan penambahan serum sapi pesisir fase berahi 20 % secara in vitro. Persentase oosit kerbau yang matang dengan penambahan serum sapi pesisir fase barahi sebanyak 20% menunjukkan perbedaan yang sangat nyata (P<0.01) yaitu 70.04% bila dibandingkan dengan pematangan dengan penambahan serum sapi pesisir 10% yaitu 56.00 %  dan tanpa serum sapi pesisir fase berahi  yaitu 36.96% secara in virto. Berdasarkan hasil penelitian dapat disimpulkan bahwa suplementasi serum sapi pesisir fase dengan konsentrasi 20% dalam media pematangan TCM-199 nyata (P<0.01) meningkatkan tingkat maturasi oosit kerbau secara in vitro dibandingkan dengan penambahan serum sapi pesisir fase berahi 10% dan tanpa penambahan serum sapi pesisir (kontrol).   Penelitian ini bertujuan untuk mengetahui persentase oosit kerbau yang matang dalam media TCM-199 yang disuplementasi serum sapi pesisir fase berahi secara in vitro. Selanjutnya untuk meningkatkan efisiensi produksi embrio in vitro pada ternak kerbau dengan suplementasi serum sapi pesisir fase berahi. Oosit ternak kerbau dimatangkan dalam media TCM-199 pada inkubator Co2 5% dan masing-masing perlakuan ditambahkan serum sapi pesisir fase berahi dengan konsentrasi yang berbeda (0%, 10% dan 20%). Peubah yang diamati dalam penelitian ini adalah persentase oosit kerbau yang matang tanpa penambahan serum sapi pesisir fase berahi, persentase oosit yang matang dengan penambahan serum sapi pesisir fase berahi 10 % dan persentase oosit kerbau yang matang dengan penambahan serum sapi pesisir fase berahi 20 % secara in vitro. Persentase oosit kerbau yang matang dengan penambahan serum sapi pesisir fase barahi sebanyak 20% menunjukkan perbedaan yang sangat nyata (P<0.01) yaitu 70.04% bila dibandingkan dengan pematangan dengan penambahan serum sapi pesisir 10% yaitu 56.00 %  dan tanpa serum sapi pesisir fase berahi  yaitu 36.96% secara in virto. Berdasarkan hasil penelitian dapat disimpulkan bahwa suplementasi serum sapi pesisir fase dengan konsentrasi 20% dalam media pematangan TCM-199 nyata (P<0.01) meningkatkan tingkat maturasi oosit kerbau secara in vitro dibandingkan dengan penambahan serum sapi pesisir fase berahi 10% dan tanpa penambahan serum sapi pesisir (kontrol). Kata kunci: in vitro, serum, sapi pesisir, fase berahi 
Kualitas Spermatozoa Epididimis Kambing Kacang dalam Bahan Pengencer Tris Kuning Telur pada Suhu 5°C Harissatria Harissatria; J. Hendri; Jaswandi Jaswandi; Hendri Hendri; F. Afrianti
Jurnal Peternakan Vol 17, No 1 (2020): Februari 2020
Publisher : State Islamic University of Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/jupet.v17i1.7125

Abstract

The purpose of this study was to observe the quality of the epididymal spermatozoa of the Kacang goats which were preserved at 5°C. Spermatozoa are taken from epididymal of Kacang goats in Payakumbuh City Slaughterhouse for 10 pairs and collected with a combination of the slicing method and suppression of the epididymal canal. The parameters observed were the percentage of progressive motility, live sperm and abnormalities. The data analyzed by Analysis of Variance in the form of a randomized block design with four treatments and six replications of the retrieval days as a group. The results showed that the percentage of progressive motility of epididymal spermatozoa after preservation of 0, 1, 2 and 3 hours was not significant (P>0.05). Furthermore, the percentage of life has a very significant effect (P<0.01) with the highest average treatment value obtained at the shelf life to 0 hours which is 66.875±19.49%. The percentage of abnormality did not have a significant effect (P>0.05). It can be concluded that the effect of preservation of different cauda epididymal semen at a temperature of 5°C has not been optimal yet in improving the quality of epididymal spermatozoa of Kacang goats.
Use of pregnant mare’s sera gonadotropin (PMSG) in media in vitro maturation of cow oocytes Zaituni Udin; Jaswandi .; Tinda Afriani; Leonardo E
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 1 (2007): MARCH 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.149 KB) | DOI: 10.14334/jitv.v12i1.564

Abstract

It is known that hormone addition in media helps in vitro maturation of oocyte. This research was aimmed to determine the effect of PMSG in media to maturation rate and nucleous developvement of cow oocyte. Ovaries were obtainned from local slaughterhouse. The media used for in vitro maturation of oocyte was TCM- 199 and the treatment was 3 levels of PMSG: 0, 10 and 20 mg/ml. Result of this research showed that the dose of PMSG in maturation media was significantly affected (P<0.05)  nucleolus development of oocytes and maturation rate. The average of germinal vesicle (GV) stage in 3 levels of PMSG 0, 10 and 20 mg/ml were 38.33; 12.64 and 9.64%, respectivelly. There was no germinal vesicle breakdown (GVBD) found in 3 levels of PMSG addition. The nucleous development of metaphase–I (M-I) were 7.64; 20.2 and 22.00%, but the average of maturation rate (M-II)  was 16.32; 48.10 and 35.34% for 3 levels of PMSG: 0, 10 and 20 mg/ml, respectivelly. It is concluded that 10 mg/ml PMSG in media of in vitro maturation resuls in the highest maturation rate of cow oocyte. Key Words: Oocyte, Maturation, In vitro, Hormone, PMSG
Post-partum reproductive appearance of buffalo at various levels of GnRH and synchronized with PGF Yendraliza .; B.P Zespin; Z Udin; Jaswandi .
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 2 (2012): JUNE 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.819 KB) | DOI: 10.14334/jitv.v17i2.684

Abstract

A study of reproductive performance of buffalo treated with levels of GnRH was conducted on twenty buffaloes of Kampar. The animals were divided into five groups. Group 1 received 200 µg GnRH, the second group received 250 µg GnRH, the third group received 300 µg GnRH, the fourth group received 350 µg GnRH and the fifth group received 400 µg GnRH. Each group also received 12.5 µg PGF2α at days 7 following GnRH injection. Sign of estrus was observed 9 days after GnRH injection. Statistical analysis was performed based on completely randomized design. Results showed that the injection of 300µg of GnRH that was followed by 12,5 µg of PGF2α was able to show obvious sign of post-partum oestrus, with sign of estrus appeared at oestrus rate of 27.8 hours, following PGF2α injection, with 100% of pregnancy rate and 100% of calving rate. Key Words: Estrus, Pregnancy Rate, Calving Rate
Nutrition quality and microbial content of buffalo, cow, and goat milk from West Sumatera S. Melia; . Yuherman; . Ferawati; . Jaswandi; H. Purwanto; E. Purwati
Jurnal Ilmu Ternak dan Veteriner Vol 23, No 3 (2018): SEPTEMBER 2018
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.817 KB) | DOI: 10.14334/jitv.v23i3.1594

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The aim of this research was to determine the quality of fresh milk physically, chemically and microbiologically obtained from cow, goats and buffalo in West Sumatra. The research method applied was laboratory experimental to analyze nutritional value, the number of aerobic bacteria and lactic acid bacteria, isolating and identifying lactic acid bacteria. Results showed that the nutritional value of milk had meet the requirements of Indonesian National Standardization, but the total colony of aerobic bacteria was above the allowed threshold of 1 x 106 CFU/ml. In addition, each sample had a total colony of varied lactic acid bacteria (LAB). The lowest total LAB value obtained in cow's milk was 0.84±0.18 x107 CFU/ml, in contrast to buffalo milk and goat milk which had a higher total LAB of 36.8±17.57 x107 CFU/ ml and 57.25±8.89 x107 CFU/ml. However, all the colonies showed almost identical morphology of LAB isolates. It is concluded that fresh milk from West Sumatra contains LAB therefore sanitation control is still needed during handling of milk.
The Manipulation effectivity of cell co-cultures in 5% CO2 incubation system to increase in vitro cattle embryo production Ferry Lismanto Syaiful; Zesfin BP; R Saladin; Jaswandi .; Hendri .
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 1 (2010): MARCH 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.437 KB) | DOI: 10.14334/jitv.v15i1.678

Abstract

The purpose of this research is to determine the effectivity of various cell co-cultures of cattle embryo production by in vitro CO2 5% incubation system and get the best cell co-culture. Cell co-culture which are used in the synthesis is the oviduct cells, isthmus cells, ampulla cells, follicle cells and without cells. Data were analyzed based on completely randomized desiggn. The average growth rate/ cleavage in various cell culture was: the oviduct cell 59.24%, ampulla cell  58.69%, isthmus cell 58.25%, follicle cell 52.24% and without cells 47.76%. The average  growth of 8-16 cells embryos to various cell co-culture was: the oviduct cell 46.02%, ampulla cell 45.45%, isthmus cell 45.15%, follicle cell 43.07%, and without cell 38.50%. The mean percentage of morula in various cell co-culture treatment was: the oviduct cell 20.59%, ampulla cell 20.48%, isthmus cell 20.30%, follicle cell 16.96% and without cell 12.58%. The average percentage of embryonic growth (cleavage, 8-16 cells and morula) was not significantly different (P > 0.05).  The treatment of a variety of cell co-culture increased significantly (P>0.05), blastocysts production, namely: the oviduct cell 3.28%, ampulla cell 3.22%, isthmus cell 3.08%, follicle cell 2.45% and without cell 1.97%.  In conclusion, the treatment of various cell co-culture in 5%CO2   incubation system can increace the growth of cattle embryos in vitro.   Key words: Cell Co-Culture, In Vitro Embryo, 5%CO2 Incubation System, Cattle
Performa Reproduksi Sapi Perah di Sumatera Barat Reswati Reswati; Jaswandi Jaswandi; E. Nurdin
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 16, No 3 (2014): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.16.3.157-165.2014

Abstract

Penelitian bertujuan untuk mengetahui performa reproduksi sapi perah di Sumatera Barat. Metode yang dilakukan adalah survey terhadap 24 ekor sapi perah yang dikelola oleh perusahaan di Kabupaten 50 Kota dan 35 ekor sapi perah yang dikelola oleh peternakan rakyat di Kota Padang Panjang. Analisis data dengan deskriptif, serta dilakukan uji T dengan program SPSS versi 12. Hasil penelitian menunjukkan umur beranak pertama (Age at First Calving) sapi perah di Kabupaten 50 Kota dan Kota Padang Panjang masing-masing 31,63±6,37 bulan dan 30,17±4,10 bulan;  masa kosong (Days Open) 260,63±147,04 hari dan 192,26±114,04 hari; sedangkan selang beranak (calving interval) 548,63±168,30 hari dan 477,26±114,04 hari. Performa reproduksi di kedua daerah ini belum baik karena melebihi jangka waktu optimal untuk ketiga variabel tersebut. Tidak ada perbedaan (P>0,05) antara penampilan reproduksi sapi perah di kedua daerah penelitian.
Pengaruh Waktu Pemberian Gonadotropin Releasing Hormone (GnRH) terhadap Jumlah Korpus Luteum dan Kecepatan Timbulnya Berahi pada Sapi Pesisir T. Afriani; Jaswandi Jaswandi; Defrinaldi Defrinaldi; Y. E. Satria
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 16, No 3 (2014): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.16.3.193-197.2014

Abstract

Penelitian ini dilakukan untuk mengetahui kecepatan timbulnya berahi dari dosis Gonadotropin Releasing Hormone (GnRH) yang berbeda dan pengaruh waktu pemberian GnRH terhadap jumlah korpus luteum pada sapi Pesisir. Materi yang digunakan yaitu 12 ekor induk sapi Pesisir, hormon PGF2α dan GnRH. Terdiri dari dua tahap, pertama Induk sapi disinkronisasi dengan 5 ml PGF2α dan kemudian diberi GnRH 50 µg, 100 µg dan 200µg. Kedua sapi disinkronisasi dengan 5 ml PGF2α dan kemudian diberi GnRH dosis hasil penelitian tahap pertama dan dibagi atas 4 waktu penyuntikan, yaitu A(16), B(32), C(48), dan D(64) jam. Peubah yang diamati adalah waktu terjadinya estrus setelah diberi dosis yang berbeda dan jumlah korpus luteum (CL) yang terbentuk dari waktu penyuntikan yang berbeda. Pengolahan data dilakukan dengan menggunakan Rancangan Acak Lengkap (RAL). Hasil penelitian menunjukkan bahwa dosis GnRH yang tercepat menunjukan gejala estrus adalah 100 µg dengan rataan 45.42±2.24 jam, 200 µg rataan 51.33±4.51 jam dan 50 µg rataan 76.53±11.92 jam. Waktu pemberian GnRH berpengaruh terhadap jumlah CL yang terlihat dari jumlah CL dari masing-masing perlakuan. Perlakuan C(48) jam menunjukkan jumlah CL yang paling banyak dengan rataan 4.33±1.15 dan diikutiB(32) jam dengan rataan 3.67±1.53,perlakuan A(16) jam dengan rataan1.67±1.15perlakuan D(64) dengan rataan 1.67±0.58.
Potensi Sapi Lokal Dalam Upaya Mewujudkan Kecukupan Daging Dan Pengembangan Kawasan Peternakan A. Agustar; Jaswandi Jaswandi
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 11, No 3 (2006): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1916.047 KB) | DOI: 10.25077/jpi.11.3.181-187.2006

Abstract

The present paper discussed the contribution of animal production on protein consumption of the people of Indonesia. The quite protein intake is mainly caused the limited sources of food protein, which was indicated by relatively high price of animal praduct for food. In the contrary, Indonesia have many kinds indegineuous livestock animals, which have became a part of socio-economic live of the people, like Bali and Pesisir cattle.
Kombinasi Minyak Atsiri Jeruk Manis dan Penisilin dengan Streptomisin pada Pengencer Semen Beku Kambing Boer S. A. Sitepu; Z. Udin; Jaswandi Jaswandi; Hendri Hendri
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 22, No 3 (2020): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.22.3.332-338.2020

Abstract

Co-Authors A Boediono A. Agustar Abdul Rahman Abimanyu, A. A. Adidin Aidin Maulana Adisti Rastosari Aditya Alqamal Alianta Aida Astiti Aji, Riyan Nugroho Alfian Asri Amrizal Anas Ananda Ananda Ananda Ananda, Ananda Ananda, Pinta Aftaprilia Rizki Anna Farhana Anna Farhana Anna Farhana Aprisal Aprisal, Aprisal arfai Arif Rahmat Auli Two Putra Syahminan B.P Zespin B.P. Zesfin Baehaqi Basyar, Basril C. Arman D. Dianti D. Mardona Damayanti, Elok Dara Surtina Defrinaldi Defrinaldi Delmita Nugrah Wati Delsi Afrini Dwi Yuzaria E. Nurdin E. Purwati Edi Set Ediset, Ediset Elfajri, Rifqi Elly Roza Elvina Endang Purwati RN Esti Rahayu F. Afrianti F. Arlina F. L. Syaiful Faizal, Noval Jumatul FAJAR HIDAYAT Faqih, M Ferawati, . Ferdinal Rahim Ferry Lismanto Syaiful Firdaus Gusdinal, H. H. Purwanto Hafrijal Syandri Harif Gusdinal Haris Satria Harissatria, Harissatria Hendri . Hendri Hendri Hendri Hendri Hendri Hendri Hendri Hendri Hendri Hendri Heriyanto, Edwin Husmaini Husmaini Husmaini, Husmaini I Gde Eka Budhiyadnya Indri Juliyarsi Indriastuti, Rhesti J. Hendri James Hellyward Jhon Hendri Jhon Hendri John Hendri John Hendri Khasrad . Leonardo E Lisna Sukmawati M A Setiadi M Ikhsan Rias M R Toelihere Mangku Mundana Mardhiyah, Indah Masrizal Masrizal Masrizal Masrizal Melati Trisnaneng Putri Mylaufa Asyraf Netti Aryani Ningsih, W. H. Putri Oktavially Putri, Atika Dwi Putri, Sri Mardhiani Penta R Saladin R. Saladin Ramadhan, R. Reswati, Reswati Rohmatulloh, Pradekita Rusdimansyah Rusdimansyah Rusfidra rusfidra Rusmana Rusmana S. Asmairicen S. Melia Sitepu, Sukma Aditya Siti Hanifah Sri Melia Tinda Afriani Windy Melinda Wulandari Wulandari Y Sukra Y. E. Satria Yendraliza - Yetmaneli, Yetmaneli Yuherman, . Yurnalis Yurnalis Yustiti, Aida Z Udin Z. Udin Z. Udin Z. Udin Zaituni Udin Zaituni Udin Zesfin BP Zuhelmi Zen Zumarni Zumarni