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- pengaruh pemberian campuran madu dan kuning telur terhadap kualitas spermatozoa,fertilitas dan daya tetas ayam sentul: pengaruh pemberian campuran madu dan kuning telur terhadap kualitas spermatozoa,fertilitas dan daya tetas ayam sentul Yustiti, Aida; Husmaini, Husmaini; Jaswandi, Jaswandi
Biospecies Vol. 14 No. 1 (2021): Januari 2021
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v14i1.10963

Abstract

Honey end egg yolk are one of the food supplements that contain antioxidants very high.This study aims to determine the quality of sentul male spermatozoa fed honey and egg yolk before artificial insemination on the quality of spermatozoa fertility hacchability of hacching eggs.This research was conducted by an experimental method with 4 levels 0 ml honey (control),3 ml honey,5 ml honey and 7 ml honey, Factor B giving egg yolk to sentul roosters with 4 levels 0 ml egg yolk (control),3 ml egg yolk,5 ml egg yolk, end 7 ml egg yolk.The results of the study showed that based on statistical analysis it was a very significant interaction between the combination of treatment levels of honey and egg yolk administraton on semen volume(0,85%),spermatozoa motility concentration(3,92x109),motility Spermatozoa(90,00%) spermatozoa abnormalities(5,00%) ,spermatozoa fertility eggs(91,67%),embryo mortality(7%),and egg hatchability(93%).From the results of the study it can be conluded that the administration of 5 ml honey and 5 ml egg yolk significantly gives the best results.
Sperm Kinematics of Pesisir Bull Thawed at Different Temperatures and Times Hendri, Hendri; Jaswandi, Jaswandi; Indriastuti, Rhesti; Ananda, Ananda
Buletin Peternakan Vol 48, No 4 (2024): BULETIN PETERNAKAN VOL. 48 (4) NOVEMBER 2024
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v48i4.96459

Abstract

Pesisir bulls from West Sumatra, Indonesia, have the potential to be valuable meat producers due to their adaptability and unique characteristics, but reproductive challenges necessitate the use of artificial insemination (AI) technology. The success of AI relies on the quality of frozen semen, which is influenced by thawing conditions, making the evaluation of sperm kinematics through Computer-Assisted Sperm Analysis (CASA) crucial for a comprehensive assessment of sperm function. This research evaluated the kinematics of frozen semen spermatozoa from Pesisir bulls. Frozen semen samples (n = 20) were thawed at 37°C for 10, 20, and 30 s and at 25°C for 30, 45, and 60 s. Kinematic parameters were observed using a Computer-Assisted Sperm Analyzer (CASA), including total motility (M), progressive motility (PM), the velocity of the average path (VAP) (?m/sec), the velocity of curvilinear (VCL) (?m/sec), the velocity of straight line (VSL) (?m/sec), straightness (STR) (%), linearity (LIN) (%), wobble (WOB) (%), amplitude of lateral head displacement (ALH) (?m), and beat/cross-frequency (BCF) (Hz). Thawed frozen semen at a temperature of 37°C for 20 s significantly (p<0.05) increased M, PM, and ALH. Furthermore, thawed frozen semen at 37°C for all durations and at 25°C for 60 s showed better quality for VAP, VCL, and VSL (p<0.05). Meanwhile, thawed frozen semen at 25°C increased STR, LIN, and WOB. However, thawing temperature and duration had no significant effect on BCF. Correlation analysis conducted on semen thawed at a temperature of 37°C for 20 s found that VAP was correlated with VCL, VSL, and ALH, while semen thawed at a temperature of 25°C for 60 s found that VAP was correlated with VCL, VSL, STR, LIN, and ALH. Most kinematic parameters were significantly better in the thawing treatment at 37°C compared to 25°C, suggesting careful consideration 
VITAMIN C SUPPLEMENTATION IN EGG YOLK CITRATE EXTENDER OPTIMIZES BANGKOK ROOSTER SEMEN QUALITY DURING COLD STORAGE Ananda; Faizal, Noval Jumatul; Jaswandi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11346

Abstract

This study aimed to evaluate the effect of different vitamin C concentrations (0, 0.1, 0.2, and 0.3 g/100 mL) in egg yolk citrate extender and storage time (0, 24, 48, 72, and 96 hours) on the quality of Bangkok rooster semen stored at 5°C. Using a split-plot-in-time design with four replications, semen quality was assessed for motility, viability, abnormality, intact plasma membrane (IPM), and longevity. Results showed a significant interaction (P<0.01) between vitamin C concentration and storage time on motility, and significant effects (P<0.05) on viability, abnormality, and IPM. The 0.2 g vitamin C dose preserved >40% motility and >50% viability for 96 hours and extended sperm longevity up to 11.25±0.95 days. These findings suggest that 0.2 g/100 mL vitamin C is the optimal concentration for preserving rooster semen quality during short-term cold storage.
Identifikasi Bakteri dan Efektivitas Antibiotik dalam Pengencer untuk Mengendalikan Pertumbuhan Bakteri pada Semen Sapi Simental Esti Rahayu; Masrizal Masrizal; Jaswandi Jaswandi; I Gde Eka Budhiyadnya
Jurnal Veteriner Vol. 26 No. 4 (2025)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/

Abstract

Kontaminasi bakteri pada semen sapi merupakan salah satu faktor yang memengaruhi kualitas dan keberhasilan program inseminasi buatan. Penelitian ini bertujuan untuk mengidentifikasi jenis bakteri yang terdapat pada semen beku sapi simental dan efektivitas variasi antibiotik dengan perlakuan pengencer berbeda. Penelitian dilaksanakan melalui tiga tahap, yaitu koleksi semen segar dari delapan ekor sapi simental di Unit Pelaksana Teknis Daerah (UPTD) Balai Pengembangan Teknologi dan Sumber Daya (BPTSD) Tuah Sakato, Payakumbuh, Sumatra Barat. Pengenceran semen dilakukan dengan tiga perlakuan (P1= tanpa antibiotik; P2= antibiotik Penicillin-Streptomycin; P3= kombinasi Gentamisin, Tilosin, Lincomisin, Spektinomisin/GTLS), serta isolasi dan identifikasi bakteri pada semen cair dan semen beku di Laboratorium Bakteriologi, Balai Veteriner Bukittinggi, Sumatra Barat. Metode isolasi menggunakan media selektif dan identifikasi dilakukan melalui pewarnaan Gram, uji motilitas, katalase, oksidase, dan uji biokimia. Hasil penelitian menunjukkan bahwa dari semen segar diisolasi 12 jenis bakteri, sedangkan dari semen beku diidentifikasi bakteri Alcaligenes spp., Chromobacterium spp., Corinebacterium spp., Klebsiella spp., Micrococcus spp.,  Alcaligenes spp., Haemophilus spp., Mycoplasma spp., Proteus spp., dan Serratia spp. Semen tanpa antibiotik (P1) memiliki tingkat kontaminasi bakteri tertinggi dengan lima jenis bakteri terdeteksi, sedangkan pada perlakuan P2 teridentifikasi tiga jenis bakteri dan P3 lima jenis bakteri namun dengan populasi lebih terkendali. Bakteri oportunistik seperti Klebsiella spp. dan Proteus spp. masih ditemukan meskipun menggunakan GTLS. Simpulannya, pada semen beku yang diencerkan dengan antibiotik masih ditemukan bakteri. Penambahan kombinasi antibiotik GTLS pada pengencer lebih efektif menekan pertumbuhan bakteri patogen dibandingkan Penicillin-Streptomycin, serta berpotensi meningkatkan mutu semen beku dan keberhasilan inseminasi buatan.
Co-Authors A Boediono A. Agustar Abdul Rahman Abimanyu, A. A. Adidin Aidin Maulana Adisti Rastosari Aditya Alqamal Alianta Aida Astiti Aji, Riyan Nugroho Alfian Asri Amrizal Anas Ananda Ananda Ananda Ananda, Ananda Ananda, Pinta Aftaprilia Rizki Anna Farhana Anna Farhana Anna Farhana Aprisal Aprisal, Aprisal arfai Arif Rahmat Auli Two Putra Syahminan B.P Zespin B.P. Zesfin Baehaqi Basyar, Basril C. Arman D. Dianti D. Mardona Damayanti, Elok Dara Surtina Defrinaldi Defrinaldi Delmita Nugrah Wati Delsi Afrini Dwi Yuzaria E. Nurdin E. Purwati Edi Set Ediset, Ediset Elfajri, Rifqi Elly Roza Elvina Endang Purwati RN Esti Rahayu F. Afrianti F. Arlina F. L. Syaiful Faizal, Noval Jumatul FAJAR HIDAYAT Faqih, M Ferawati, . Ferdinal Rahim Ferry Lismanto Syaiful Firdaus Gusdinal, H. H. Purwanto Hafrijal Syandri Harif Gusdinal Haris Satria Harissatria, Harissatria Hendri . Hendri Hendri Hendri Hendri Hendri Hendri Hendri Hendri Hendri Hendri Heriyanto, Edwin Husmaini Husmaini Husmaini, Husmaini I Gde Eka Budhiyadnya Indri Juliyarsi Indriastuti, Rhesti J. Hendri James Hellyward Jhon Hendri Jhon Hendri John Hendri John Hendri Khasrad . Leonardo E Lisna Sukmawati M A Setiadi M Ikhsan Rias M R Toelihere Mangku Mundana Mardhiyah, Indah Masrizal Masrizal Masrizal Masrizal Melati Trisnaneng Putri Mylaufa Asyraf Netti Aryani Ningsih, W. H. Putri Oktavially Putri, Atika Dwi Putri, Sri Mardhiani Penta R Saladin R. Saladin Ramadhan, R. Reswati, Reswati Rohmatulloh, Pradekita Rusdimansyah Rusdimansyah Rusfidra rusfidra Rusmana Rusmana S. Asmairicen S. Melia Sitepu, Sukma Aditya Siti Hanifah Sri Melia Tinda Afriani Windy Melinda Wulandari Wulandari Y Sukra Y. E. Satria Yendraliza - Yetmaneli, Yetmaneli Yuherman, . Yurnalis Yurnalis Yustiti, Aida Z Udin Z. Udin Z. Udin Z. Udin Zaituni Udin Zaituni Udin Zesfin BP Zuhelmi Zen Zumarni Zumarni