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Maria Selvester Thadeus
Universitas Pembangunan Nasional Veteran Jakarta
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Journal : BIOEDUSCIENCE

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In Vitro Antifungal Activity of Ethanolic Stem Extract of Tinospora crispa Against Candida albicans and Malassezia furfur Mumtaz, Syifa Afifah; Zulfa, Fajriati; Thadeus, Maria Selvester; Setyaningsih, Yuni
BIOEDUSCIENCE Vol 9 No 3 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/17984

Abstract

Background: Fungal infections such as candidiasis caused by Candida albicans and pityriasis versicolor caused by Malassezia furfur are highly prevalent in Indonesia. Conventional antifungal treatments often face challenges like resistance and adverse side effects. As an alternative, brotowali stem (Tinospora crispa) extract, rich in alkaloids, flavonoids, and tannins, shows promise as an antifungal agent. This research investigates the in vitro antifungal efficacy of ethanol extract from brotowali stem against C. albicans and M. furfur. Methods: A laboratory experimental design utilizing the well-diffusion method was applied, measuring inhibition zone diameters on Sabouraud Dextrose Agar after 2x24 hours at extract concentrations of 45%, 60%, 75%, and 90%. Results: The ethanol extract of brotowali stem exhibited statistically significant but relatively low antifungal activity based on inhibition zone diameters against C. albicans (0,002) and M. furfur (0,001), with higher concentrations showing greater antifungal. The largest inhibition zones measured 5.93 mm for C. albicans and 3.97 mm for M. furfur. Conclusions: The extract concentrations of 90% concentration exhibited moderate antifungal activity against C. albicans. For M. furfur, all concentrations demonstrated weak antifungal activity.
Co-Authors Abdullah Rukman Adisaputra, Chantika Zahra Adrian Dharmawan Adyatma, Hilmi Agneta Irmarahayu Akbar, Rafi Thoriq Alyani, Fadhilah Sharfina Annisa Afifah Audrey Alvura Digna Basuki Supartono Cahyaninglatri, Wendi Corlia Cut Fauziah Cut Fauziah Cut Fauziah Dimas Farhan Wibawanto Dinda Nuraini Hanifah Wahab Eko Satrio Bahari Erna Harfiani Fachruddin Perdana Fajriati Zulfa Fajriati Zulfa Familia Bella Rahadiati Giopratiwi, Kusvandita Hany Yusmaini Hardini, Niniek Hartono, Riyadi Hikmah Muktamiroh Husna Bunga Jannati Jannati, Husna Bunga Kristina Simanjuntak Kristina Simanjuntak Kristina Simanjuntak Marlina Dewiastuti, Marlina Meiskha Bahar Mila Citrawati Muhammad Alif Farhan Muhammad Kevin Auliansyah Akbar Muktamiroh Hikmah Mumtaz, Syifa Afifah Muttia Amalia Nabila, Inasa Nadira Safa Jasmine Nainggolan, Dorlina Nazira, Syarifah Ni Luh Aurial Widjayanti Niniek Hardini Niniek Hardini Noor Az Zhahra Nooramadhani, Tasya Nugrahayu Widyawardani Nunuk Nugrohowati Nurfitri Bustamam Pamuji, Alifia Shafanaura Pasiak, Taufiq Fredrik Prakoso, Luthfi Octafyan Pujangga, Ibnu Wadud Purba, Raja Soaloon Rahadiati, Familia Bella Ramadhansyah, Reza Reinhart Fikram Imam Akbar Retno Yulianti Retno Yuliyanti Rukman Abdullah Ryan Herardi Salman Paris Salman Paris Setyaningsih, Yuni Simanjuntak, Kristina Soroy Lardo Sri Wahyuningsih Sugeng Wiyono Sukrisno, Adi Tiwuk Susantiningsih Tri Faranita, Tri Uswatun Hasanah Uswatun Hasanah Wahab, Dinda Nuraini Hanifah Wibawanto, Dimas Farhan Yanto Sandy Tjang Yudhi Nugraha Yuliyanti, Retno