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All Journal Jurnal Akuakultur Indonesia Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Omni-Akuatika Jurnal Agroqua: Media Informasi Agronomi dan Budidaya Perairan Jurnal Perikanan Universitas Gadjah Mada Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Jurnal Kelautan dan Perikanan Terapan (JKPT) Journal of Aquaculture Science Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Journal of Aquaculture and Fish Health Jurnal Penyuluhan Perikanan dan Kelautan Jurnal Abdi Insani Buletin Jalanidhitah Sarva Jivitam Journal of Fisheries & Marine JARI : Jurnal Akuakultur Rawa Indonesia
Mulyono, Mugi
Politeknik Ahli Usaha Perikanan
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Evaluation of commercial Pacific white shrimp Litopenaeus vannamei (Boone, 1931) feeds: growth performance and body carcass analysis Rheido, Gestar; Novriadi, Romi; Suhardi, Muhammad Tegar Alif; Suharyadi, Suharyadi; Sektiana, Sinar Pagi; Margono, Margono; Mulyono, Mugi
Journal Omni-Akuatika Vol 18, No 1 (2022): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2022.18.1.925

Abstract

Growth performance, survival rate, productivity, and carcass analysis were examined in the whole grow-out production system for Pacific white shrimp Litopenaeus vannamei fed four commercial diets over 15 weeks. Shrimp were held in twelve hapas nets with a size of 2 x 2 x 1 m and installed within the commercial ponds. The shrimp were stocked with the density of 100 shrimp m-2 per net in a completely randomized design (CRD). All feed used in this study was characterized with high protein levels within the range of 40 – 42% labeled as HP A and B; and medium protein levels (30 – 35%), labeled as MP A and B. Based on the proximate and nutritional profile analysis of the diet, the nutritional profile of HP B, MP A and MP B written on the feed bags showed an inconsistent results compared to the results of the test.  Shrimp fed the highest protein level had higher final body weight; feed conversion ratio, thermal growth coefficient, survival rate and average daily growth compared to MP B. Shrimp fed higher protein level also exhibited the lowest feed conversion ratio compared to the group of shrimp fed with medium protein. The results obtained in this study indicated that with small difference in growth rates, especially between HP groups compared with MP A, there is still a chance to re-formulate the diet to produce a cost-effective diet that still fulfill the specific nutrient requirement of the shrimp. Feed did not influence the nutritional deposition of the whole body of shrimp, but it seemed due to the inappropriate drying techniques Keywords: Protein level, growth, carcass, Litopenaeus vannamei, specific nutrient
Karakteristik Fisikokimia dan Aktivitas Antioksidan Hidrolisat Protein Ikan Kakap (Lutjanus sp.) Skala Pilot Yuniarti, Tatty; Arrahmi, Nafa Ya’la; Dharmayanti, Niken; Sugiwati, Sri; Mulyono, Mugi; Hidayat, Taufik; Martosuyono, Pujoyuwono; Maulani, Aghitia; Alghany, Albar
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 1 (2024): Juni 2024
Publisher : Politeknik - Ahli Usaha Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v19i1.985

Abstract

Hidrolisat protein ikan (HPI) merupakan produk dari reaksi hidrolisis ikatan peptida menghasilkan pelepasan peptida aktif pendek dan lebih mudah diserap. Ikan kakap merupakan komoditas perikanan laut yang berlimpah di Indonesia. Penelitian ini bertujuan untuk menentukan karakteristik fisikokimia dan kemampuan bioaktif HPI dari ikan kakap merah (Lutjanus sp.) pada skala pilot (biofermentor 120 kg). Pengujian bahan baku dan HPI yang dilakukan meliputi uji organoleptik, uji TVB, uji ALT, uji proksimat. pembuatan HPI dilakukan dengan menggunakan enzim alkalase 20.000 U/kg substrat saat suhu 55 °C dengan waktu hidrolisis pada jam ke-0 hingga jam ke-8. Pengujian derajat hidrolisis dan uji aktivitas antioksidan metode 2,2-difenil-1-pikrilhidrazil (DPPH) dilakukan pada tiap jam. Hasil penelitian menunjukkan waktu hidrolisis mulai stabil pada jam ke-6 hingga jam ke-8. Derajat hidrolisis (DH) HPI pada jam ke-6 adalah sebesar 13,3% dan inhibisi antioksidan sebesar 6,75%. Bahan baku HPI merupakan ikan segar dengan nilai TVB 12,89%; kadar protein 21,41%; lemak 4,66%; air 72,36%; dan abu 0,95%. Komposisi kimia HPI memiliki kadar protein 19,20%; lemak 0,15%; air 0.06%; abu 1,15%. Rendemen HPI yang dihasilkan sebesar 15,75% dari bahan baku. Total mikroba dan organoleptik memenuhi standar SNI. Kandungan asam amino tertinggi dari bahan baku dan HPI merupakan asam glutamat dengan nilai masing-masing 3,3% dan 3,4%. Kandungan asam amino terendah pada bahan baku dan HPI adalah histidin dengan nilai masing-masing 0,43% dan 0,52%. Produksi HPI skala pilot menghasilkan HPI yang kaya asam amino esensial dan memiliki aktivitas antioksidan, sehingga berpotensi digunakan sebagai ingridient pangan fungsional.KATA KUNCI: AbstractFish protein hydrolysate (FPH) is a product of the hydrolysis reaction of peptide bonds resulting in the release of short active peptides and more easily absorbed. Fish protein hydrolyzate (HPI) is a product of the hydrolysis reaction of peptide bonds resulting in the release of short active peptides that are more easily absorbed. Snapper is an abundant marine fishery commodity in Indonesia. The aim of this study was to determine the physicochemical characteristics and bioactive capabilities of HPI from red snapper (Lutjanus sp.) on a pilot scale (120 kg biofermenter). Raw material and HPI testing includes organoleptic tests, TVB tests, ALT tests, proximate tests; HPI production uses the alcalase enzyme 20,000 U/kg substrate at a temperature of 55°C with a hydrolysis time of 0 to 8 hours. Tests for the degree of hydrolysis and antioxidant activity tests using the DPPH method are carried out every hour. The results showed that the hydrolysis time began to stabilize at the 6th to the 8th hour. The value of DH HPI at the 6th hour was 13.3% and antioxidant inhibition was 6.75%. The raw material is fresh fish with a TVB of 12.89%; protein content 21.41%; fat 4.66%; water 72.36%; ash 0.95%. The resulting HPI has a protein content of 19.20%; fat 0.15%; water 0.06%; ash 1.15%. HPI yield 15.75% of raw materials. Total microbes and organoleptics meet SNI standards. The highest amino acid content of raw materials and HPI is glutamic acid with values of 3.3% and 3.4% respectively. The lowest amino acid content in raw materials and HPI is histidine with values of 0.43% and 0.52% respectively. Pilot scale HPI production produces HPI which is rich in essential amino acids and has bioactive antioxidant capabilities, so it has the potential to be used as a functional food ingredient.
CALLUS INDUCTION IN Kappaphycus alvarezii USING INDOLE-3-ACETIC ACID (IAA) and 6-BENZYLAMINOPURINE (BAP) FOR SEEDSTOCK DEVELOPMENT Mulyono, Mugi; Salsabila, Mutia Safa; Rasnijal, Muhammad; Fadilah, Siti; Putra, Angkasa
Indonesian Aquaculture Journal Vol 20, No 1 (2025): (June, 2025)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.20.1.2025.1-10

Abstract

The commercially important red seaweed Kappaphycus alvarezii is extensively cultivated for carrageenan production. Despite its economic value, large-scale reproduction and genetic enhancement remain limited due to its low regeneration potential. This study aimed to optimize plant growth regulator (PGR) concentrations for efficient callus induction in K. alvarezii. A completely randomized design was employed, comprising five treatments with varying concentrations of indole-3-acetic acid (IAA) and 6-benzylaminopurine (BAP), along with a control lacking PGRs. A total of 180 explants from meristematic tissues of acclimatized thalli were cultured (30 explants per treatment). The highest callus induction rate (88%) was achieved with 1.50 mg/L IAA and 5 mg/L BAP (Treatment F), with visible callus formation beginning around day 38. A progressive color change from brown to white was observed, indicating active cellular proliferation. Other treatments exhibited lower induction rates, ranging from 0% (control) to 61% (Treatment D). These findings underscore the critical influence of auxin–cytokinin interactions on callogenesis and offer an optimized hormonal regime for improving in vitro culture efficiency. The established protocol provides a valuable platform for future large-scale propagation and genetic improvement strategies in K. alvarezii, contributing to the advancement of seaweed biotechnology.
PENGARUH PENGGUNAAN BENTUK SISTEM KOMPARTEMEN INDIVIDU YANG BERBEDA TERHADAP TOTAL HAEMOCYTE COUNT DAN PERTUMBUHAN LOBSTER PASIR (Panulirus homarus) Putri, Sayira Yuliantari Ardian; Mulyono, Mugi; Sektiana, Sinar Pagi; Soebjakto, Slamet; Bahrawi, Samsul
Jurnal Riset Akuakultur Vol 17, No 3 (2022): (September) 2022
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.17.3.2022.179-190

Abstract

Tujuan penelitian ini adalah untuk mengetahui pengaruh penggunaan bentuk sistem kompartemen individu (SKI) yang berbeda terhadap total haemocyte count (THC) dan kinerja produksi lobster pasir (Panulirus homarus). Penelitian meliputi persiapan wadah, penebaran benih, pengelolaan pakan, monitoring kualitas air, monitoring pertumbuhan, pengendalian hama dan penyakit, panen, dan pemeriksaan THC. Penelitian dilakukan secara eksperimental dengan rancangan acak lengkap (RAL). Penelitian ini terdiri atas tiga perlakuan dan tiga ulangan yaitu menggunakan SKI tabung, SKI prisma segitiga, dan SKI kubus. Nilai terbaik ditemukan pada penggunaan SKI tabung dibanding SKI prisma segitiga dan SKI kubus dengan THC 1,33 ± 0,12 x 106 sel ml-1, survival rate 83%, feed conversion ratio 9,63 ± 0,48, pertumbuhan bobot 51,83 ± 5,35 g ekor-1, pertumbuhan panjang 7,05 ± 0,09 cm ekor-1, dan specific growth rate 1,50 ± 0,07 % hari-1. Hasil penelitian ini menyimpulkan bahwa perbedaan bentuk SKI memengaruhi kinerja produksi dan tingkat stress lobster budidaya.The purpose of this study was to determine the effects of the use of different shapes of individual compartment system (ICS) on the total haemocyte count (THC) and growth performances of sand lobster (Panulirus homarus). The research included container preparation, seed stocking, feed management, water quality monitoring, growth monitoring, pest and disease control, harvesting, and the examination of THC. The experimental units of the study were arranged using a completely randomized design (CRD). This study employed three treatments and three replications, i.e., tube ICS, triangular prism ICS, and cube ICS. The best values were found in the use of tube ICS compared to triangular prism ICS and cube ICS with a THC of 1.33 ± 0.12 x 106 cells ml-1, a survival rate of 83%, a feed conversion ratio of 9.63 ± 0.48, a weight growth of 51.83 ± 5.35 g individual-1, a length growth of 7.05 ± 0.09 cm individual-1, and a specific growth rate of 1.50 ± 0.07% day-1. The results of this study concluded that differences in ICS shapes affected the production performances and the stress level of the cultivated lobster.
PELATIHAN BUDIDAYA RUMPUT LAUT BERKELANJUTAN SISTEM KARAMBA KEPADA PEMBUDIDAYA DAN PENYULUH PERIKANAN Mulyono, Mugi; Ilham, Ilham; Suharyadi, Suharyadi; Marlina, Erni; Samsuharapan, Sri Budiani; Damanik, Lina Aftiani
Jurnal Abdi Insani Vol 11 No 2 (2024): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v11i2.1553

Abstract

This training is to increase knowledge and skills in seaweed farming activities. Training is carried out with theory in the classroom and hands-on practice in the field. The training phase in class is based on theory and discussion of problems that occur in the cultivation business. At the field practice stage a performance is carried out so that farmers are able to do and apply between theory and practice. The results of the training can be directly evaluated through training in the practice of planting seaweed to harvest. In general, at the three experimental sites, kreteria growth of seaweed. The karamba system method can be a problem in handling the problem of pests and seaweed damage, this is evidenced from 3 test groups of general problems growing and developing.
Aplikasi Pemberian Dosis Pupuk Provasoli’s Enriched Seawater (PES) Yang Berbeda Pada Produksi Bibit Gracillaria verrucosa Melalui Kultur Jaringan Dengan Metode Propagasi Vegetatif Rasnijal, Muhammad; Alauddin, Muhammad Hery Riyadi; Budiyati; Anton; Muhammad Syahrir; Yunarty; Saridu, Siti Aisyah; Wahid, Eriyanti; Regan, Yip; Hardianto, Toto; Supryady; Ihwan; Ernawati; Anwar; Mulato, Alwi; Sucipto; Syarief, Muhammad Nurman; Suleman, Yakub; Andini, Salsa; Suleman, Gabriella Augustine; Mulyono, Mugi; Anggoro, Agung Doni; Sektiana, Sinar Pagi; Achmad Suhermanto
JARI : Jurnal Akuakultur Rawa Indonesia Vol. 13 No. 2 (2025): JARI: JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Program Studi Budidaya Perairan, Fakultas Pertanian, Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/jari.v13i2.71

Abstract

Cultivation of the seaweed Gracilaria verrucosa is necessary as a preventive measure against overexploitation. One applicable method is tissue culture, which requires special attention to nutrient needs to support accelerated growth. These nutrients can be provided through fertilization. One commonly used chemical fertilizer in seaweed tissue culture is Provasoli’s Enriched Seawater (PES). This study aims to determine the optimal PES fertilizer dosage for the production of G. verrucosa seedlings through tissue culture using a vegetative propagation method. This study used a completely randomized design with analysis of variance (ANOVA) consisting of four treatments of different PES fertilizer doses with three replicates for each treatment. The results showed that fertilizer dose variation did not significantly affect the absolute growth of explant weight, but tended to increase the number of growth points. Growth points began to increase in the second week for all treatments, and by the fourth week, the number of growth points at a 1.5% dosage showed a significant difference compared to other doses. The application of PES fertilizer in G. verrucosa seedling production through tissue culture with vegetative propagation indicates that different fertilizer doses do not affect explant growth but significantly influence the increase in growth points by the fourth week
OPTIMASI PEMUPUKAN ORGANIK DAN ANORGANIK UNTUK MENINGKATKAN KUALITAS KARAGENAN RUMPUT LAUT (Kappaphycus alvarezii) Aufat, Agung Gunawan; Mulyono, Mugi; Sektiana, Sinar Pagi
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 21, No 4 (2025): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.21.4.271-276

Abstract

Kappaphycus alvarezii adalah komoditas budidaya laut yang memiliki nilai ekonomi tinggi sebagai sumber karagenan, senyawa hidrokoloid yang banyak digunakan dalam industri pangan, farmasi, dan kosmetik. Permintaan global terhadap karagenan yang terus meningkat memerlukan penerapan teknik budidaya yang tidak hanya meningkatkan hasil panen, tetapi juga kualitas produk. Penelitian ini bertujuan untuk menganalisis pengaruh pemberian pupuk organik dan anorganik terhadap kualitas karagenan yang dihasilkan dalam budidaya K. alvarezii . Perlakuan yang diberikan meliputi pupuk organik cair D.I.GROW, pupuk anorganik NPK, pupuk urea, dan kontrol tanpa pupuk. Metode yang digunakan adalah eksperimen dengan rancangan acak lengkap (RAL), di mana setiap perlakuan diterapkan pada kelompok yang terpisah dan diuji berdasarkan parameter rendemen, viskositas, kadar air, dan kadar abu. Hasil penelitian menunjukkan bahwa perlakuan dengan D.I.GROW menghasilkan rendemen sebesar 41% dan viskositas 189,37 cP, yang keduanya melebihi standar yang direkomendasikan oleh FAO. Sebaliknya, kontrol tanpa pupuk menghasilkan nilai terendah pada seluruh parameter yang diuji. Kadar air pada kontrol mencapai 42%, melebihi batas standar FAO yang merekomendasikan kadar air maksimal 30% untuk produk karagenan berkualitas tinggi. Perlakuan dengan pupuk lainnya masih memenuhi standar, dengan kadar air berada pada kisaran yang disarankan, yaitu sekitar 30%. Kadar abu pada perlakuan NPK dan urea berada dalam kisaran standar (20-30%), sementara D.I.GROW dan kontrol menunjukkan kadar abu yang lebih rendah, yang menunjukkan kualitas kemurnian produk yang lebih baik. Secara keseluruhan, penelitian ini membuktikan bahwa pemberian pupuk berpengaruh signifikan terhadap kualitas karagenan K. alvarezii , dengan pupuk D.I.GROW terbukti lebih efektif dalam meningkatkan viskositas, rendemen, dan kemurnian produk, sementara pupuk anorganik lebih berperan dalam menjaga kestabilan karakteristik fisik karagenan.
Co-Authors Afandi Saputra Alauddin, Muhammad Hery Riyadi Alghany, Albar Amyda Suryati Panjaitan Amyda Suryati Panjaitan Amyda Suryati Panjaitan Andini, Salsa Anggoro, Agung Doni Angkasa Putra Anton Anton Anton Anwar Ardana Kurniaji Arrahmi, Nafa Ya’la Asy Syafa Yumna Aufat, Agung Gunawan Bahrawi, Samsul Bambang Gunadi Budiyati Budiyati Budiyati, Budiyati Damanik, Lina Aftiani Dharmayanti, Niken Djumbuh Rukmono Edi Suharyadi Effi Aftiani Thaib Effi Athfiyani Thaib Ernawati Erni Marlina Erni Marlina, Erni Farchan, Mochammad Faridatun Hasanah Fitriska Hapsyari Hardianto, Toto Ihwan IIN SITI DJUNAIDAH Ilham Ilham Ilham Ilham Ilham Ilham Ita Junita Puspa Dewi Lubis, Amelia Sriwahyuni M.Si, Niken Dharmayanti Margono Margono Maria GE Kristiany Maria Goreti Eny Kristiany Martosuyono, Pujoyuwono Maulani, Aghitia Moch Farchan Mochammad Farkan Mochammad Farkan Muchtar Muchtar Muchtar Muchtar Muhammad Syahrir Mulato, Alwi Nanda Anggiani Putri Novira Nanda Tasyah Nur Rafa Madihahrahma Otie Dylan Soebhakti Hasan Otie Dylan Subhakti Hasan Pujoyuwono Martosuyono Putra, Angkasa Putri Renitasari, Diana Putri, Sayira Yuliantari Ardian Rahardjo, Sinung Raihan Maulana Firdaus Rasnijal, Muhammad Regan, Yip Retno Ayu Mawarti Rheido, Gestar Ridwan, Ridwan Romi Novriadi Salsabila, Mutia Safa Samsuharapan, Sri Budiani Saridu, Siti Aisyah Sektiana, Sinar Pagi Sinar Pagi Sektiana Sinung Rahardjo siti fadilah Soebjakto, Slamet Sri Budiani Samsuharapan Sri Sugiwati Subakti Hasan, Otie Dylan Sucipto Sugiwati, Sri Suhardi, Muhammad Tegar Alif Suharyadi Suharyadi Suharyadi Suharyadi Suhermanto, Achmad Suleman, Gabriella Augustine Suleman, Yakub Supryady Syarief, Muhammad Nurman Taufik Hidayat Ulya Syofroul Lailiyah Umidayati, Umidayati Vini Taru Febriani Prajayati Wahid, Eriyanti Yamina Safitri Yunarty Yuniarti, Tatty