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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Science and Technology Indonesia Jurnal Ilmu Kefarmasian Indonesia Gema Wiralodra Jurnal Kefarmasian AKFARINDO Jurnal Abdimas Ilmiah Citra Bakti (JAICB) Indonesian Journal of Pharmaceutical Education Journal of Social Research Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Ruang Cendekia : Jurnal Pengabdian Kepada Masyarakat Sciences of Pharmacy Jurnal Ilmiah Farmasi Simplisia Journal of Natural Product for Degenerative Diseases
RAHMAT, DENI
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In Silico and In Vitro Antibiofilm Activity of Zingiber montanum Ethyl Acetate Fraction Against Propionibacterium acnes: Antibiofilm of Zingiber montanum Aji, Nur; Kumala, Shirly; Mumpuni, Esti; Rahmat, Deni
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

Propionibacterium acnes (P. acnes), a symbiotic bacterium involved in the development of acne, is recognized for its capacity to develop resistance due to its ability to generate biofilms. According to a previous investigation, the ethyl acetate fraction derived from Zingiber montanum exhibited anti-bacterial properties against P. acnes. Nevertheless, the causative role of biofilm formation in its resistance was not previously recognized. This study aims to ascertain the antibiofilm efficacy using in silico and in vivo methodologies. In this study, in silico examination utilized molecular docking to inhibit the quorum sensing mechanism by blocking the autoinducer 2 (AI-2) signal. The target protein was AI-2 binding periplasmic protein LuxP (PDB code: 4YRZ). Following that, an in vitro test was conducted using the crystal violet staining method, which was read using a microplate reader at a wavelength of 600 nm. The readings were expressed as percent inhibition and IC50. The in silico results showed that there were six compounds in the ethyl acetate fraction that have the potential to inhibit AI-2 signaling. Curcumin showed the highest inhibitory activity. Meanwhile, the in vitro results revealed that the ethyl acetate fraction of Z. montanum obtained the minimum biofilm inhibitory concentration (MBIC) of 2.5 % (w/v) and IC50 = 0.28 % (w/v). It can be concluded that the ethyl acetate fraction of Zingiber montanum could inhibit the biofilm formation of P. acnes.  
Co-Authors . Syamsudin Ahmad Faried Aji, Nur Akhsani, Luthfia Wikhdatul Aulena, Desi Nadya Azis, Rizal Chaidir Chaidir Datubara, Martaulina Elisabet Dian Ratih Laksmitawati Didik Priyandoko Djami, Ratna Djamil, Ratna Dwi Fitriyani, Dwi Effionora Anwar Elisabeth, Mariska Errol Rakhmad Noordam Esti Mumpuni, Esti Girsang, Ermi Hadiprasetyo, Dhanar Septyawan Harjanti, Mathelda Weni Harry Murti Haryanti, Mathelda Weni Hermawati, Ema Indarwati Indarwati Jeffrey ., Jeffrey Komala, Ismiarni Marisca Evalina Gondokesumo, Marisca Evalina Nainggolan, Ita Margaretha Nancy Dewi Yuliana Nelly Suryani Ni Made Dwi Sandhiutami Nindya, Faradhina Salfa Ofa Suzanti Betha Panca, Pra Panca Bayu Chandra Pra Panca Bayu Chandra Prayoga, Tria Puspitasari, Ranny Putra, Riza Dharma Qlintang, Sandy Raihan, Dany Sabrina Dahlizar Saufa, Zahra Qisthi Shirly Kumala Sinuhaji, Indra Sofa Fajriah Sugiastuti, Fenty Swandiny, Greesty F Swandiny, Greesty Finotory Syamsudin Syamsudin Takasenserang, Oktaviana Taurhesia, Shelly Teresa Liliana Wargasetia Triharsiwi, Dwi Nur Utami, Usi Hikmah Vinna Kurniawati Sugiaman, Vinna Kurniawati WAHYU WIDOWATI Yesi Desmiaty, Yesi Zahara, Indah Zahiroh, Fadhilah Haifa Zaidan, Sarah