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All Journal EKSAKTA: Journal of Sciences and Data Analysis Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Biotropika Indonesian Green Technology Journal The Journal of Pure and Applied Chemistry Research Journal of Mathematical and Fundamental Sciences JURNAL CITRA KEPERAWATAN Indonesian Journal of Chemistry Indonesian Food Science and TechnologyJournal Berdikari: Jurnal Pengabdian Masyarakat Indonesia International Journal of Community Service Learning JKPK (Jurnal Kimia dan Pendidikan Kimia) JSMARTech : Journal of Smart Bioprospecting and Technology Journal of Applied Veterinary Science and Technology Berkala Penelitian Hayati Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) Makara Journal of Science Pustaka: Jurnal Bahasa dan Pendidikan Bagimu Negeri : Jurnal Pengabdian Kepada Masyarakat Engagement: Jurnal Pengabdian Kepada Masyarakat DIVERSITY: Journal of Multi Science Linearity Journal of Tropical Diversity
Safitri, Anna
Brawijaya University
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Journal : Biotropika

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Genomic Profile of OsCOMT in Indonesian Purple Rice Wijayanti, Ernanin Dyah; Safitri, Anna; Siswanto, Dian; Fatchiyah, Fatchiyah
Biotropika: Journal of Tropical Biology Vol. 10 No. 3 (2022)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.03.04

Abstract

The Indonesian purple rice (IPR), a crossbreeding of black and white rice, is a potential source of ferulic acid. Up to this point, the genomic similarity between the crossbreeding and its parentals has not been determined, particularly in genes involved in ferulic acid biosynthesis. In this present work, we analysed the profile of Oryza sativa caffeic acid-O-methyltransferase (OsCOMT) gene in IPR. The genomic DNA was extracted by CTAB method, then amplified with a specific primer of OsCOMT gene. The amplicon was sequenced by Sanger method and confirmed by geneID NC_029259.1. The IPR showed an amplicon of 708 bp sequence of the OsCOMT gene, indicating the gene involved in ferulic acid biosynthesis. The IPR gene profile is similar to the parentals, but there are variations in point mutations that distinguish it from the parentals. Aspartic acid was changed to Glutamine by mutations at positions 52, 69, and 79. We suggest that IPR has a novel OsCOMT gene variant that is unique as it is the crossbreed.
Co-Authors Ainun Sajidah Ajeng Erika Prihastuti Haskito Akhmad Sabarudin Aldila Noviatri Almayda, Nabila Anna Roosdiana Anna Roosdiana Anna Roosdiana Anna Roosdiana Anna Roosdiana Annisa, Choirin Arie Srihardyastutie Arief, Miftahudin Ary Mustofa Ahmad Asri Nurul Huda Aulanni`am Aulanni`am Avander Alexander Nuban Aviva Levina Aviva Levina Budi Kamulyan Cairns, James Robert Ketudat Chanif Mahdi Chanif Mahdi Citra Sari Dewi Ratih Tirto Sari Dian Siswanto Dwi Putri Woro Pamungkas Dzakiyyah Salmaa Eko Suyanto Ellya Indahyanti Ellysia Hitdatania Ernanin Dyah Wijayanti Eva Nur Laili Octaviana Fadlila, Safina Samara Nur Fairus Suryani Munir fatchiyah . Fatchiyah Fatchiyah Fatchiyah Fatchiyah Fatchiyah Fatchiyah Fatchiyah Fatchiyah Fatchiyah Fatchiyah Fatimah, Wahyu Dwi Febrianti, Wiwin Dwi Firza Rajasa Gunawan Gatrananda, Sayyid Izzudin Gunawan, Firza Rajasa Handayu Untari Haskito, Ajeng Erika Prihastuti Irfan Mustafa Irfan Mustafa James Robert Ketudat Cairns Lay, Peter A. Layta Dinira Levina, Aviva Lidwina Faraline Triprisila Lidwina Faraline Triprisila Masruri Masruri Muhrimah, Siti Nariswari, Bintari nasrullah wilutono Nia Kurnianingsih Nia Kurniawan Ningsih, Zubaidah Novi Kurnianingsih Noviatri, Aldila Oktarini, Dwi Oktavia Eka Puspita, Oktavia Eka Peter A Lay Peter A Lay Purnamasari, Citra Putri, Radwa Fathin H Rahmaniah, Galuh Rensani Taloin Rista Nikmatu Rohmah Saniyawati, Fang You Dwi Ayu Shalu Sari, Dewi Ratih Tirto Sasangka Prasetyawan Sasangka Prasetyawan Sasangka Prasetyawan Savira Ayu Damayanti Senge, Yeyen Susana Siti Mariyah Ulfa Sovia Rosalin Sri Wardhani Suci Amaliyah Sunarni Sunarni Sutrisno Sutrisno Sutrisno, Sutrisno Tri Wahyu Nugroho Ulfa Andayani Ulfa Andayani Untari, Handayu Yenny Kornitasari Yoravika Dwiwibangga Yufani, Rofika An Zahara, Izaz Aqeiluz