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Ashari, Ayu
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Journal : Borneo Journal of Medical Laboratory Technology

 1 
Aktivitas Antibakteri Kombinasi Ekstrak Biji Manjakani (Quercus infectoria) Dan Daun Sirih Merah (Piper crocatum) Terhadap Pertumbuhan Bakteri Klebsiella pneumoniae: Antibacterial Activity Combination of Manjakani Seed Extract (Quercus infectoria) and Red Betel Leaf (Piper crocatum) Against the Growth of Klebsiella pneumoniae. Ashari, Ayu; Harlita, Tiara Dini; Aina, Ganea Qorry
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6075

Abstract

Potential plants found in Indonesia that can be used for the treatment of urinary tract infections include: Manjakani seeds (Quercus infectoria) and red betel leaves (Piper crocatum). Manjakani seeds are pharmacologically claimed to have biological activities such as anti-inflammatory, antibacterial and antifungal. Red betel leaf has the potential as an antibacterial in the treatment of infectious diseases. The purpose of this study was to determine the antibacterial activity of the combination of manjakani seed extract and red betel leaf in inhibiting the growth of Klebsiella pneumoniae bacteria. The type of research used istrue experiment study with a completely randomized design (CRD) with three factors, namely the type of solvent n-hexane, ethyl acetate, ethanol 96%, ratio (1:1,1:2,2:1) and concentration (75%,100%) with each treatment being repeated twice. This research was conducted in the microbiology laboratory, Labkes, East Kalimantan. The population in this study is the manjakani plant and red betel plant and tested using the agar diffusion technique method Kirby Bauer with positive control (Ciprofloxacin) and negative control (aquadest). Data analysis used is One Way ANOVA and advanced test methods Duncan with a significance level of 0,05. The results obtained showed that there was an effect of antibacterial activity of Manjakani seed extract and red betel leaf on growth K. pneumoniae. The most effective concentration was found in ethanol 96% extract, (2:1 combination of 100% concentration) of 13,97 mm and an effectiveness of 55,74% with a positive control ciprofloxacin 5µg of 25,06 mm. It can be concluded that the combined extract of manjakani seeds and red betel leaves has antibacterial activity against bacteria Klebsiella pneumoniae,but the potential is still low of ciprofloxacin.
Co-Authors A.Y. Soegeng Adelena Agiwahyuanto, Faik Agushybana , Farid Ahmad Hanafie Aina, Ganea Qorry Al Fitri, Muhammad Rizqi Al Mefa, Fadira Al zami, Farrikh Alzami, Farrikh Atari, Nabila Azkiya, Nur Anita Azzura, Aika cantika, clarisa martila Cerlyawati, Hugi Dzuha Hening Yanuarsari, Dzuha Hening Eti Rimawati Fajri, Muhammad Taufik Feny Marselina Fitri, Ichlasia Ainul Fitria Wulandari Hadi, Heru Pramono Haidar, Kadori Hakam, Mochamad Abdul Hallang Lewa, Andi Hamdan Arfandy Hanafiah, Akhmad Harahap, Ade Chita Putri Hastuti Agussalim Husna, Najwa Ida Farida Ilyas, Nita Magfirah Jaka Prasetya Juli Ratnawati Karis Widyatmoko Kris Setyaningsih, Kris Kusumawati, Nugraheni Kusumawati, Yupie Lewa, Andi Hallang Megantara, Rama Aria Moh. Yusuf Mr Sjaeful Anwar Mudzanatun Muhammad Naufal, Muhammad Muhammad Yunus Munawwarah Munawwarah Nafira, Fathan Azfa Nakayaanni, Putri Nazella, Desvita Dian Nazira Dhani, Zahra Ninik Christiani Nur Hikmah Nuridzin, Dion Zein Omay Sumarna Padapi, Astrini Pirawati Prihandono, Adi prizkila, cici Puji Purwatiningsih, Aris R.B. D, Keukeu Amalia Rabial Kanada, Rabial Rahmayani, Hafsah Dahni Rambe, Afghan Bai Asy Ary Retno Astuti Setijaningsih Reza, Reza Rezky, Sri Rezky,  Sri Rohman, M. Hilma Minanur Safitri, Maria Santri, Ayu Sarqawi, Ahmad Sasono Wibowo Setiawan, Aries Siramaneerat, Issara Siti Zubaidah Sofiyanti, Ida Sugiarti, S. Suharnawi Suharnawi Sukamto, Titien Suhartini Sumiati Side SUTRISNO Tambunan, Rahma Sari Putri Tiara Dini Harlita Tuzahra, Sunia Umar, Bowasis wahyuni , kiki Wibowo, Syifa Sofia Widitya, Galuh Zaenal Arifin Zay, Fany Pebriani