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All Journal Media Kedokteran Hewan Jurnal Medik Veteriner Veterinary Biomedical and Clinical Journal Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Journal of Pharmaceutical and Sciences.
Hidajati, Nove
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary

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Journal : journal of Basic Medical Veterinary

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DESCRIPTION OF HEMATOLOGICAL VALUE IN LEOPARD GECKO (Eublepharis macularius) Wiranata, Try Ardhi; Widjiati, Widjiati; Wahyuni, Retno Sri; Hidajati, Nove; Poetranto, Emmanuel Djoko; Yuliani, M. Gandul Atik
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19990

Abstract

The aim of this research was to explore the hematological value of leopard gecko (Eublepharis macularius). Total of 20 samples were examined, its consists of 10 males and 10 females, blood samples were taken from vena ventral abdominal and deposited into EDTA tubes, fresh blood leaves little to make blood smear, total blood examination conducted with automatic hematological analyzer ABX MICROS 60 and for total leukocyte and differential leukocyte count used standard manual method. The mean result of hematological value of leopard gecko in this research are WBC (x103/mm3) 7,12 male, 8,09 female, Lymphocyte (%) 46,2 male, 38,4 female, Monocyte (%) 17 male, 17,3 female, Heterophil (%) 18,3 male, 21,8 female, Eosinophil (%) 10,8 male, 13,9 female, Basofil (%) 7,7 male, 8,6 female, RBC (106/mm3) 0,64 male, 0,65 female, HGB (g/dl) 8,95 male, 9,46 female, Hematocrit (%) 10,55 male and 9,26 female. In all parameters showed that hematology value of male gecko leopard was lower than female gecko except on two parameters which are hematocrit and lymphocyte which showed male gecko has the higher value than females, but the hematology value difference of both sexes leopard gecko is not significant except in heterophil value. 
THE EFFECT OF POLYPROPYLENE PLASTIC RESIDUE ON HEATED PALM OIL AGAINST HISTOPATHOLOGICAL CHANGES OF SMALL INTESTINE ON MALE WHITE RAT (Rattus norvegicus) WISTAR STRAIN Amaris, Aulia Puspa; Yustinasari, Lita Rakhma; Legowo, Djoko; Plumeriastuti, Hani; Anwar, Chairul; Hidajati, Nove
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20409

Abstract

This study was conducted to investigate the effect of polypropylene residue on heated palm oil against histopathological changes of small intestine on male white rat (Rattus norvegicus) wistar strain. Eighteen male white rats divided into three groups and were administered via peroral gavage with different treatments for five weeks. The treatment were Control (84 grams palm oil), P1 (84 grams palm oil with 2.3 grams plastic), and P2 (84 grams palm oil with 6.81 grams plastic). The histopathological changes of small intestine were examined by using Barthel Scoring method was analysed using Kruskal Wallis and continued with Mann-Whitney test. The result showed that were significant difference (p<0.05) between treatment groups. The worse damage of polypropylene plastic residue was 6.81 grams.
THE EFFECT OF POLYPROPYLENE PLASTIC RESIDUE ON HEATED PALM OIL AGAINST THE HISTOPATHOLOGICAL CHANGES OF KIDNEY ON MALE RATS (Rattus norvegicus) Cahyono, Shahani Azpriyanne; Eliyani, Hana; Widiyatno, Thomas Valentinus; Plumeriastuti, Hani; Hidajati, Nove; Santoso, Kuncoro Puguh
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20415

Abstract

This research was conducted to observe the effect of polypropylene plastic residue on heated palm oil against the histopathological changes on rat's kidney. There were 18 male rats (Rattus norvegicus) that used as the examination animal and were divided into three groups and were given the mixture of heated palm oil and polypropylene plastic per oral with intubation needle. The treatment was done for five weeks. The treatment for P0 group was 84 g of palm oil, for P1 was 84 g of palm oil with 2.3 g of plastic, and for the P2 was 84 g of palm oil with 6.8 g of plastic. The histopathological changes of the kidney were observed using microscope with 400x magnification and the data was analysed using Kruskal-Wallis and continued with Mann Whitney U test. The result showed that there were significant differences between three groups of treatment (P<0.05). There were effects of polypropylene plastic residue on heated palm oil against the histopathological changes of kidney in this research such as cast, epithelial sloughing and necrosis in the mild scale.
Identification of Cat (Felis catus) Blood Splatter on Cotton Fabric After Periods of Drying Using Leucomalachite Green and Takayama Reagent Charles, Vanessa Ann; Lestari, Tita Damayanti; Legowo, Djoko; Ismudiono, Ismudiono; Hidajati, Nove; Wahyuni, Retno Sri
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28593

Abstract

Blood-stain or blood splatter analysis when used properly can assist in establishing a chain of events linked to violent crimes (Bevel and Gardner, 2008). The methods used in detecting blood splatters in the field are chemical methods. Leucomalachite green is a colorimetric test which is used to test the presence of blood (Castro and Coyle, 2008). Takayama reagent is a confirmatory test for blood (Strassman, 1922). The aim of this research is to detect the blood splatter on cotton fabric after it has been dried for 1 day, 3 days and 5 days using Leucomalachite green and Takayama reagent. Cotton fabric was specifically chosen for this experiment with 3 different periods of drying. The unstained cotton fabric was cut into squares, and a blood sample was splattered on each piece. The fabrics splattered with blood were then dried for 1 day, 3 days and 5 days. The blood splatter was then tested using Leucomalachite green and Takayama reagent, and the results were noted afterwards. For the control, red food dye was dried for 1 day then tested with Leucomalachite green and Takayama reagent. The image results of the Leucomalachite green test are analyzed using ImageJ software 1.8.0_112 where the red, green and blue pixels are converted to grayscale. The image results of the Takayama test are graded based on the number and pattern of crystal. In conclusion, Leucomalachite green and Takayama reagent are able to detect cat blood splatter on the cotton fabric. Leucomalachite green produced a higher intensity/ darker colour as a result of an older sample, and the lower intensity/ lighter colour as a result of a fresher sample of the Leucomalachite green test. Takayama reagent produced a densely packed pattern of crystals as a result of an older sample, and the loosely packed pattern of crystals as a result of a fresher sample of the Takayama test.
Toxicity Testing Of White Pomegranate (Punica granatum L.) Fruit Extracts Using Brine Shrimp Lethality Test Method As A Candidate Of Anti-Cancer Drug Firnanda, Ferian; Herupradoto, Eduardus Bimo Aksono; Rahmawati, Kadek; Kurnijasanti, Rochmah; Sukmanadi, Mohammad; Hidajati, Nove
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31810

Abstract

This research aimed to determine the effects of toxic white pomegranate fruit extract (Punica granatum L) against larvae of brine shrimp (Artemia salina Leach) indicated LC50 values below 1000 µg/ml. This study is purely experimental by using Brine Shrimp Lethality Test (BSLT). The study was divided into seven groups, namely ethanol extract of white pomegranate fruit (Punica granatum L) with a concentration of 31,25; 62,5; 125; 250; 500; 1000 µg/ml and negative control (seawater). Mortality data percentage of Artemia salina Leach analyzed by probit analysis. Results showed that the extract of white pomegranate fruit extract (Punica Granatum L) has a toxic effect with LC50 values of 248,6 µg/ml calculate from probit analysis. From these results, it can conclude that white pomegranate extract is toxic to larval shrimp (Artemia salina Leach) with Brine Shrimp Lethality Test (BSLT) method which means white pomegranate extract has the potential to be an anticancer drug.
Co-Authors Abdul Samik, Abdul Aksono HP., Eduardus Bimo Al-Zuhroh, Tisya Yumn Amaris, Aulia Puspa Ananda, Hanifah Muslimah Budi Utomo Cahyono, Shahani Azpriyanne Chairul Anwar Charles, Vanessa Ann Eka Pramyrtha Hestianah, Eka Pramyrtha Faisal Fikri Farah Ghifara Hafidha Firnanda, Ferian Gandul Atik Yuliani Hana Eliyani Hani Plumeriastuti Hariadi, Mas'ud Herry Agoes Hermadi Hidayatik, Nanik Indah Norma Triana, Indah Norma Ismudiono Ismudiono, Ismudiono Johansyah, Fadilla Anita Kinksky Legowo, Djoko Lilik Maslachah Muhammad Thohawi Elziyad Purnama Poetranto, Emmanuel Djoko Praja, Ratih Novita Rahmawati, Kadek Rimayanti Rimayanti Rochmah Kurnijasanti ROHMAH, LAILATUL Santoso, Kuncoro Puguh Saputro, Amung Logam Soeharsono Suherni Susilowati Sukmanadi, Moh. Sukmanadi, Mohammad Sulaiman, Azhari Athaillah Sunarso, Agus Suzanita Utama Tita Damayanti Lestari Tjuk Imam Restiadi Wahyuni, Retno Sri Widiyatno, Thomas Valentinus Widjiati Widjiati, Widjiati Wiranata, Try Ardhi Wisudawati, Feby Dwi Wiwik Misaco Yuniarti Wurlina, W Yunita, Maya Nurwartanti Yustinasari, Lita Rakhma