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Kadar Zinc Serum Penderita Psoriasis Tutik Rahayu; Eka Putra W; Marsita Endy D; Rina Diana; Nugrohoaji Dharmawan
Cermin Dunia Kedokteran Vol. 48 No. 3 (2021): Obstetri - Ginekologi
Publisher : PT Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55175/cdk.v48i3.48

Latar Belakang: Psoriasis adalah penyakit autoimun dengan dasar genetik dan dicetuskan oleh berbagai faktor di antaranya stresor fisik, stresor kimia, dan pajanan bahan-bahan kimia. Pembentukan radikal bebas dalam tubuh dapat dihambat oleh zinc. Tujuan: Untuk mengetahui kadar zinc serum penderita psoriasis. Metode: Penelitian analitik komparatif numerik dengan rancangan penelitian potong lintang. Sampel terdiri dari 10 orang penderita psoriasis dan 10 orang kontrol. Analisis sampel serum darah untuk pemeriksaan kadar zinc menggunakan inductively couple plasma-mass spectrometry (ICP-AES). Analisis statistik menggunakan uji independent sample T-Test dan bermakna jika nilai p<0,05.Hasil: Kadar zinc serum penderita psoriasis lebih rendah dibandingkan kontrol (p = 0,02). Background: Psoriasis is an autoimmune disease with a genetic basis and triggered by several factors such as physical stressors, chemical stressors, and exposure of chemical agent. The formation of free radicals can be inhibited by zinc. Objective: To determine serum zinc level among psoriasis patients. Methods: An analysis with numerical comparative, coss-sectional study design. Samples were 10 psoriasis and 10 non psoriasis patients. Analysis of blood serum samples for zinc levels used inductively couple plasma-mass spectrometry (ICP-AES). Statistical analysis used independent sample T-test with significant value p<0.05. Results: The serum zinc level was significantly lower in psoriasis patients compared to non-psoriasis group (p = 0.02).

The differences of blood type in relation to psoriasis and its onset: Cross sectional study Dharmawan, Nugrohoaji; Widhiati, Suci; Oktavriana, Triasari; Harahap, Irene Jessica Pinarsinta
Journal of General - Procedural Dermatology & Venereology Indonesia Vol. 5, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Background: Psoriasis is a chronic skin disease caused by inflammatory response and attacks 2% of population, with the prevalence influenced by age, geographical location, and genetic background. Until this day, there are several studies that discuss about the relationship between blood type with disease. However, discussion about the difference between blood type and the relationship with skin disease remains limited. The objective of this study is to know the relationship between blood type, psoriasis, and onset of psoriasis.Methods: This study adopted cross-sectional design of analytic observational study. The subjects of this study were patients in Department of Dermatology and Venereology at Dr. Moewardi General Hospital and UNS Hopital in Surakarta. The blood types were determined by two methods; by using ABD-Pad measurement tools that able to see the reaction of antigen and antibody in short time and by using the hospital’s laboratory facility where sample was taken. Anamnesis were done to collect data about the onset of psoriasis. Chi-square (X2) statistical test was used to analyze the data.Results: Total sample of this study were 62 people with 31 subjects (50%) were psoriasis patients and 31 others (50%) are non-psoriasis patients. Chi-square test (X2) between blood type with psoriasis and blood type with the onset of psoriasis are p = 0.269 and p = 0.188 respectively. Conclusion: The significance level used was p < 0.05, therefore there’s no significant correlation between blood type with psoriasis and the onset of psoriasis.

The Association of Keloid Site with its Histopathological Features: an Analytical Observational Study Wibisono Nugraha; Muhammad Eko Irawanto; Moerbono Mochtar; Nur Rachmat Mulianto; Novan Adi Setyawan; Nugrohoaji Dharmawan
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 36 No. 1 (2024): APRIL
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bikk.V36.1.2024.47-52

Background: Keloid is a growth of fibrous tissue in the wound tissue of susceptible individuals. This tissue extends beyond the boundaries of the previous wound. The site of keloids commonly appears on a high-tension area, such as the chest, shoulders, and neck. Histopathologically keloids show thickened the epidermis and the vascularization and infiltration of inflammatory cells in the dermis. Purpose: This study aims to determine the relationship between the site of keloids and the histopathological appearance of keloids. Methods: An analytical observational study was conducted on keloid patients visiting the Dermatovenereology outpatient clinic of Dr.Moewardi Hospital. The sample collection used consecutive sampling techniques Result: The majority of keloid patients are >30 years old (46.4%). Most patients with keloids were female (53.6%). Keloids were mostly found on the chest (25.0%). Tounge-like appearance of the epidermis at the edges of the lesions was mostly on the shoulders and chest (33.3% each, p=0.048); flattened appearance was found on the middle epidermis of the lesions, which were mostly on the ears, shoulders, and upper extremities (22.7% each, p=0.011). Increased vascularity was found in the dermis at the edges of the lesions, especially in the ear and chest areas (31.3% each, p=0.046). Moderate-severe inflammatory infiltrates in the dermis at the edges of the lesions were commonly found on the chest (p=0.04). Conclusion: There is a significant relationship between the site of the lesion and the histopathological appearance of the keloid in epidermal as well as dermal layers.

Ulkus Marjolin yang Berkembang dari Ulkus Trofik Kronis pada Pasien Kusta Adniana Nareswari; Harijono Kariosentono; Nugrohoaji Dharmawan; Muhammad Eko Irawanto; Nurrachmat Mulianto
MEDICINUS Vol. 34 No. 2 (2021): MEDICINUS
Publisher : PT Dexa Medica

Marjolin’s ulcer is a malignant complication of chronic ulcer. Changes in ulcer lesions to malignancy can be caused by chronic irritation, infection, decreased vascular variation, and increased expression of proto-oncogene. The most common histopathological form of Marjolin's ulcer is squamous cell carcinoma. A 55-year-old man, complaining of a protruding wound on his left hand that has not healed since three years ago. The patient was diagnosed with leprosy 30 years ago with a history of incomplete treatment. On physical examination, the left palmar region showed solitary ulcer, ±10x8x1.5 cm in size, nodular surface, reddish, hard consistency, with bulging and irregular edge. Slit-skin smear (SSS) examination with Ziehl-Neelsen staining found abundant acid-fast bacilli. Histopathological examination of the ulcer edge shows appearance of epithelial tumor, with differentiated epidermal cells invading the dermis, tumor cells arranged focally and concentrically with a mass of keratin appeared as horn pearl cells. Fite-Faraco staining found abundant M. leprae acid-fast bacilli. Based on the patient’s medical history and clinical examination, patient was diagnosed with Marjolin's ulcer in multibacillary (MB) leprosy. Patient was treated with MB multidrug therapy for 12 months and below-wrist amputation.

Terapi Oral dan Tetes Mata Topikal pada Green Nail Syndrome Putti Fatiharani Dewi; Ambar Aliwardani; Nugrohoaji Dharmawan
MEDICINUS Vol. 34 No. 3 (2021): MEDICINUS
Publisher : PT Dexa Medica

Background: Green nail syndrome is one of the chromonychia caused by infection of Pseudomonas aeruginosa, characterized by onycholysis and greenish-black discoloration of the nail. There were no reports regarding the incidence of green nail syndrome in Indonesia. Predisposing factors for the occurrence of green nail syndrome include onychomycosis, high activity in moist and wet conditions, diabetes mellitus, and immunosuppression. Case: A 51-year-old man complained about greenish-black discoloration on his fingernails. Dermatological examination in 1st and 3rd digit of the right hand, as well as 1st digit of the left hand, found multiple greenish-black discoloration with onychodystrophy. Dermoscopy examination of the affected nails showed greenish-black pigmentation in the distolateral nails with peripheral fading. Culture examination using MacConkey agar showed colonies of greenish-brown pigments. The patient was diagnosed with green nail syndrome and treated with ciprofloxacin 500 mg/day and topical polymixin B eye drops b.i.d. for 3 weeks, resulting in good outcome. Discussion: The diagnosis of green nail syndrome is made based on anamnesis, physical examination, and bacterial culture. showing a distinctive feature. The predisposition factor in this case, is the frequent washing hands habit of the patient up to more than ten times per day. Treatment using topical eye drops facilitates the absorption of active ingredients into the nails.

Kromoblastomikosis Penyakit Jamur yang Terabaikan Danu Yuliarto; Achmad Satya Negara; Harijono Kariosentono; Nugrohoaji Dharmawan; Nurrachmat Mulianto
MEDICINUS Vol. 36 No. 1 (2023): MEDICINUS
Publisher : PT Dexa Medica

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56951/medicinus.v36i1.116

Chromoblastomycosis is a deep fungal infection of the skin and subcutaneous tissue caused by pigmented or dematiaceous fungi. This disease is endemic in tropical and subtropical regions such as Asia, Africa, and Latin America. Chromoblastomycosis is more common in adult men who work in agricultural areas, work as gardeners, or as carpenters. The diagnosis of chromoblastomycosis is confirmed by the finding of muriform cells or medlar bodies obtained from skin scrapings, microscopic culture, or tissue histopathological examination. The clinical feature varies, the initial lesion is a papule that spreads slowly over several months or years, and then this lesion will form a plaque with atrophic center. Many patients go undiagnosed at the early stage of the disease because this stage is rarely seen. Treatment of chromoblastomycosis includes surgery for the initial lesion and pharmacological therapy using antifungal agent such as itraconazole, the most commonly used drug for the treatment of chromoblastomycosis. The prognosis for chromoblastomycosis is poor, except on new lesions.

Correlation of CD4+ levels and caspase-3 in condyloma acuminata with HIV reactive patients Nurfaiqoh, Elok; Mawardi, Prasetyadi; Dharmawan, Nugrohoaji; Julianto, Indah; Ellistasari, Endra Yustin
MEDISAINS Vol 22, No 1 (2024)
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/medisains.v22i1.21501

Background: Human papillomavirus is an infection that causes malignancy because of persistency and modulation of apoptotic pathways, especially caspase-3. Factors that can increase persistency, recurrency, and malignancy of HPV infection include HIV infection with low CD4 levels. There is disagreement or deep molecular understanding of the induction and modulation of apoptosis in HIV-mediated CD4+ T cell depletion, especially in CA. However, it is necessary to see how CD4+ levels can influence caspase-3, so it may open up new avenues for supporting investigation to consider the presence of malignancy or therapeutic strategies regarding CD4+ can induce apoptosis.Purpose: This study aimed to determine whether there is a correlation between CD4+ levels and caspase-3 expression in condyloma acuminata with HIV reactive patients.Methods: This is an analytic observational study with a cross-sectional design. The research was conducted at Dr. Moewardi General Hospital from August to December 2023. Nineteen patients with condyloma acuminata and HIV reactive were included in this study with a consecutive sampling technique. The expression of caspase-3 was assessed using immunohistochemical staining, looking at the percentage of stained cell nuclei and cytoplasm and CD4+ levels with flow cytometry examination—data analysis using Pearson correlation.Results: Respondents in this study were primarily men, self-employed with heterosexual orientation and genito-genital-oral sexual intercourse. The statistical analysis showed no significant relationship (p: 0.300, r: -0.251) between CD4+ levels and caspase-3 expression.Conclusions: While not statistically significant, CD4+ level is reduced in correlation with increased caspase-3 expressions.

Topical Fermented Lactobacillus acidophilus Lysate Accelerates Second-Degree Burn Healing: An In Vivo Study in Wistar Rats Kamilah, Lian; Kusumawardani, Arie; Widhiati, Suci; Dharmawan, Nugrohoaji; Ellistasari, Endra Yustin
Open Access Indonesian Journal of Medical Reviews Vol. 5 No. 4 (2025): Open Access Indonesian Journal of Medical Reviews
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/oaijmr.v5i4.725

Second-degree burns, affecting the epidermis and dermis, constitute a major category of thermal injuries globally, presenting significant clinical challenges including pain, infection risk, and potential scarring. While standard treatments like silver sulfadiazine (SSD) exist, limitations including potential cytotoxicity and emerging resistance necessitate exploration of novel therapeutic strategies. Recent interest has focused on topical applications derived from probiotics, such as Lactobacillus spp., due to their suggested roles in modulating inflammation, combating oxidative stress, and providing antimicrobial activity to accelerate wound repair. This study investigated a fermented lysate derived from Lactobacillus acidophilus (L. fermented) as a potential topical agent for burn healing. This study aimed to evaluate the therapeutic efficacy of a 5% topical L. fermented ointment on the healing process of experimentally induced second-degree burns in a Wistar rat model, primarily by assessing the rate of wound closure compared to standard SSD treatment and an untreated control. A true experimental in vivo study utilizing a post-test only control group design was performed following ethical approval. Fifteen male Wistar rats were subjected to a standardized second-degree thermal burn injury on their dorsal aspect. The animals were then randomized (n=5 per group) to receive twice-daily topical applications of either 5% L. fermented ointment (Group A), SSD ointment (Group B), or no treatment (Control, Group C). Wound healing was quantitatively assessed by measuring the wound surface area (mm²) on days 1, 3, 7, and 14 post-injury using digital imaging and ImageJ software analysis. Statistical comparisons between groups were conducted using one-way ANOVA followed by LSD post-hoc tests, with statistical significance set at p<0.05. All treatment groups demonstrated a significant reduction in wound size over the 14-day observation period (p=0.001 within each group). Inter-group comparisons revealed significantly accelerated wound closure in Group A starting from day 3 onwards (p<0.005). At day 14, the mean wound area in Group A (17.5 ± 8.06 mm²) was significantly smaller than in Group B (119.22 ± 45.41 mm²) and Group C (305.18 ± 25.21 mm²) (p=0.001). Post-hoc analysis confirmed the superiority of L. fermented treatment over both SSD (mean difference 101.72 mm², p=0.001) and control (mean difference 287.68 mm², p=0.001). SSD treatment also resulted in significantly better healing than the control group (mean difference 185.96 mm², p=0.001). In conclusion, topical application of 5% fermented Lactobacillus acidophilus lysate significantly accelerated the closure of second-degree burn wounds in Wistar rats, demonstrating superior efficacy compared to both silver sulfadiazine treatment and no treatment. These findings highlight the potential of L. fermented lysate as a promising novel therapeutic agent for burn wound management.

Kadar Zinc Serum Penderita Psoriasis Tutik Rahayu; Eka Putra W; Marsita Endy D; Rina Diana; Nugrohoaji Dharmawan
Cermin Dunia Kedokteran Vol 48 No 3 (2021): Obstetri - Ginekologi
Publisher : PT Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55175/cdk.v48i3.48

Latar Belakang: Psoriasis adalah penyakit autoimun dengan dasar genetik dan dicetuskan oleh berbagai faktor di antaranya stresor fisik, stresor kimia, dan pajanan bahan-bahan kimia. Pembentukan radikal bebas dalam tubuh dapat dihambat oleh zinc. Tujuan: Untuk mengetahui kadar zinc serum penderita psoriasis. Metode: Penelitian analitik komparatif numerik dengan rancangan penelitian potong lintang. Sampel terdiri dari 10 orang penderita psoriasis dan 10 orang kontrol. Analisis sampel serum darah untuk pemeriksaan kadar zinc menggunakan inductively couple plasma-mass spectrometry (ICP-AES). Analisis statistik menggunakan uji independent sample T-Test dan bermakna jika nilai p<0,05.Hasil: Kadar zinc serum penderita psoriasis lebih rendah dibandingkan kontrol (p = 0,02). Background: Psoriasis is an autoimmune disease with a genetic basis and triggered by several factors such as physical stressors, chemical stressors, and exposure of chemical agent. The formation of free radicals can be inhibited by zinc. Objective: To determine serum zinc level among psoriasis patients. Methods: An analysis with numerical comparative, coss-sectional study design. Samples were 10 psoriasis and 10 non psoriasis patients. Analysis of blood serum samples for zinc levels used inductively couple plasma-mass spectrometry (ICP-AES). Statistical analysis used independent sample T-test with significant value p<0.05. Results: The serum zinc level was significantly lower in psoriasis patients compared to non-psoriasis group (p = 0.02).

Platelet-Rich Plasma-Derived Exosomes Modulate Follicular Regeneration: A Comparative Mechanistic Analysis with Minoxidil in a Preclinical Model of Androgenetic Alopecia Trya Oktaviani; Arie Kusumawardani; Suci Widhiati; Nugrohoaji Dharmawan; Endra Yustin Ellistasari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 11 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i11.1437

Background: The therapeutic armamentarium for androgenetic alopecia (AGA) is limited, with variable efficacy and potential side effects associated with standard treatments like minoxidil. Platelet-rich plasma-derived exosomes (PRP-Exo) represent a novel acellular strategy, offering a concentrated payload of regenerative biomolecules. This study aimed to rigorously evaluate the therapeutic efficacy and underlying mechanisms of PRP-Exo, as a monotherapy and in combination with minoxidil, in a validated murine model of AGA. Methods: A parallel-group, randomized, double-blind, controlled experimental study was conducted. Thirty-two male C57BL/6 mice with testosterone-induced AGA were randomized (n=8/group) to one of four groups: Negative Control (NC), Positive Control (PC; 5% topical minoxidil), Treatment 1 (T1; intradermal PRP-Exo), or Treatment 2 (T2; combination of PRP-Exo and minoxidil). PRP-Exo were characterized by Transmission Electron Microscopy, Nanoparticle Tracking Analysis, and ELISA for marker proteins. After a 14-day treatment period, efficacy was assessed via hair follicle density (HFD), anagen-to-telogen (A/T) ratio, and hair shaft thickness. Mechanistic insight was obtained by quantifying tissue protein levels of Ki-67 and β-catenin by ELISA. Results: All active treatments significantly improved hair regeneration compared to the NC group. The combination therapy (T2) demonstrated the most profound effects across all metrics, showing statistically superior outcomes compared to both minoxidil (PC) and PRP-Exo (T1) monotherapies in HFD (65.8 ± 12.1 vs. 36.2 ± 8.5 and 47.3 ± 10.4 follicles/mm², respectively; p<0.01). Furthermore, T2 treatment led to the highest A/T ratio and hair shaft thickness. ELISA revealed that T2 treatment also resulted in the highest tissue concentrations of the proliferation marker Ki-67 and the Wnt pathway protein β-catenin, suggesting enhanced mitogenic activity and modulation of key developmental pathways. Conclusion: PRP-Exo is a potent hair regenerative agent, significantly outperforming minoxidil in this preclinical model. The combination of PRP-Exo and minoxidil exhibits a synergistic effect, promoting superior follicular regeneration by concurrently stimulating tissue proliferation and upregulating key components of the anagen-promoting Wnt signaling pathway. These findings underscore the significant clinical potential of PRP-Exo as a next-generation therapy for AGA.

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