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Topical Fermented Lactobacillus acidophilus Lysate Accelerates Second-Degree Burn Healing: An In Vivo Study in Wistar Rats Kamilah, Lian; Kusumawardani, Arie; Widhiati, Suci; Dharmawan, Nugrohoaji; Ellistasari, Endra Yustin
Open Access Indonesian Journal of Medical Reviews Vol. 5 No. 4 (2025): Open Access Indonesian Journal of Medical Reviews
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/oaijmr.v5i4.725

Second-degree burns, affecting the epidermis and dermis, constitute a major category of thermal injuries globally, presenting significant clinical challenges including pain, infection risk, and potential scarring. While standard treatments like silver sulfadiazine (SSD) exist, limitations including potential cytotoxicity and emerging resistance necessitate exploration of novel therapeutic strategies. Recent interest has focused on topical applications derived from probiotics, such as Lactobacillus spp., due to their suggested roles in modulating inflammation, combating oxidative stress, and providing antimicrobial activity to accelerate wound repair. This study investigated a fermented lysate derived from Lactobacillus acidophilus (L. fermented) as a potential topical agent for burn healing. This study aimed to evaluate the therapeutic efficacy of a 5% topical L. fermented ointment on the healing process of experimentally induced second-degree burns in a Wistar rat model, primarily by assessing the rate of wound closure compared to standard SSD treatment and an untreated control. A true experimental in vivo study utilizing a post-test only control group design was performed following ethical approval. Fifteen male Wistar rats were subjected to a standardized second-degree thermal burn injury on their dorsal aspect. The animals were then randomized (n=5 per group) to receive twice-daily topical applications of either 5% L. fermented ointment (Group A), SSD ointment (Group B), or no treatment (Control, Group C). Wound healing was quantitatively assessed by measuring the wound surface area (mm²) on days 1, 3, 7, and 14 post-injury using digital imaging and ImageJ software analysis. Statistical comparisons between groups were conducted using one-way ANOVA followed by LSD post-hoc tests, with statistical significance set at p<0.05. All treatment groups demonstrated a significant reduction in wound size over the 14-day observation period (p=0.001 within each group). Inter-group comparisons revealed significantly accelerated wound closure in Group A starting from day 3 onwards (p<0.005). At day 14, the mean wound area in Group A (17.5 ± 8.06 mm²) was significantly smaller than in Group B (119.22 ± 45.41 mm²) and Group C (305.18 ± 25.21 mm²) (p=0.001). Post-hoc analysis confirmed the superiority of L. fermented treatment over both SSD (mean difference 101.72 mm², p=0.001) and control (mean difference 287.68 mm², p=0.001). SSD treatment also resulted in significantly better healing than the control group (mean difference 185.96 mm², p=0.001). In conclusion, topical application of 5% fermented Lactobacillus acidophilus lysate significantly accelerated the closure of second-degree burn wounds in Wistar rats, demonstrating superior efficacy compared to both silver sulfadiazine treatment and no treatment. These findings highlight the potential of L. fermented lysate as a promising novel therapeutic agent for burn wound management.

Reduction of Interleukin-6 after PRP-Exosomes Treatment in A Mouse Model of Androgenetic Alopecia Ellistasari, Endra Yustin; Widhiati, Suci; Julianto, Indah; Rosmarwati, Ervina; Monica, Pristia Widya; Yasmin, Aulia
Proceeding ISETH (International Summit on Science, Technology, and Humanity) 2024: Proceeding ISETH (International Summit on Science, Technology, and Humanity)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/iseth.5508

Purpose: This study aim to determine the role of platelet-rich plasma (PRP) exosomes as anti-inflammatory (IL-6) in a mouse model of androgenetic alopecia. Methodology: There are four group of androgenetic alopecia mice model with no treatment group (control group, K1), group treated with PRP-exosomes (K2), group treated with 5% topical minoxidil (K3) and the last group treated with combination of PRP-exosomes and 5% topical minoxidil (K4). All of the group was evaluated clinically and immunohistochemical examination of IL-6 was performed on the 32nd day. Results: Better hair growth results were obtained in the treatment group compared to the control group. In clinical evaluation, K4 had the best results, followed by K2 and K3. The expression of IL-6 is highest in the K1, followed by K4, K2 and K3 and statistically significant. Applications/Originality/Value: PRP-exosomes can enhance hair growth in androgenetic alopecia mice model. IL-6 expression in the control group is significantly higher than in the treatment group.

STUDI RETROSPEKTIF HEMANGIOMA INFANTIL DI RUMAH SAKIT PUSAT RUJUKAN DI SURAKARTA JAWA TENGAH Widhiati, Suci; Rahma, Alfina; Ellistasari, Endra Yustin; Nareswari, Adniana
Media Dermato-Venereologica Indonesiana Vol 52 No 2 (2025): Media Dermato Venereologica Indonesiana
Publisher : Perhimpunan Dokter Spesialis Kulit dan Kelamin Indonesia (PERDOSKI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33820/mdvi.v52i2.398

Pendahuluan: Hemangioma infantil (HI) merupakan tumor jinak vaskular yang muncul dalam beberapa minggu pertama kehidupan. Prevalensi HI secara global sekitar 4,5%. Umumnya HI tidak memerlukan terapi dan mengalami regresi spontan, namun 10-15% akan mengakibatkan komplikasi seperti obstruksi, ulserasi atau cacat. Tujuan penelitian ini untuk mengetahui karakteristik pasien baru HI di instalasi rawat jalan RSUD Dr. Moewardi Surakarta periode 1 Januari 2019 - 31 Desember 2021. Metode: Metode penelitian ini merupakan retrospektif dengan desain potong lintang, Data diambil dari rekam medis pasien hemangioma yang datang ke Instalasi Rawat Jalan RSUD Dr. Moewardi Surakarta periode 1 Januari 2019 - 31 Desember 2021. Hasil: Terdapat 135 pasien yang didiagnosis HI dengan kisaran usia 7 hari hingga 13 tahun. Kelompok usia terbanyak adalah 0-1 tahun sebanyak 69 pasien (51,11%), perempuan sebanyak 88 pasien (65,19%). Awitan terbanyak usia < 2 bulan yaitu 94 pasien (69,63%). Ujud kelainan kulit terbanyak berupa nodul sebanyak 101 pasien (74,81%), soliter pada 124 pasien (91,85%). Predileksi pada kepala dan leher sebanyak 76 pasien (56,29%). Pasien lahir aterm sebanyak 111 pasien (82,22%), berat badan lahir cukup 123 pasien (91,11%). Riwayat persalinan pervaginam 100 pasien (74,07%). Modalitas terapi terbanyak propranolol pada 37 pasien (27,42%). Kesimpulan: Gambaran HI di RSUD Dr. Moewardi Surakarta berdasarkan awitan, predileksi, jumlah lesi, manifestasi klinis dan terapi sesuai dengan deskripsi teori, namun faktor risiko yang diteliti tidak sejalan dengan teori.

Optimizing Sunburn Prevention in Children: A Meta-Analysis of the Efficacy of Sunscreen Application, Protective Apparel, and Shade-Seeking Behaviors Ellistasari, Endra Yustin; Suci Widhiati; Sesia Pradestine
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 8 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i8.1355

Background: Childhood sunburn significantly elevates lifelong skin cancer risk, underscoring the need for effective prevention. While sunscreen, protective apparel, and shade-seeking are advocated, a quantitative synthesis of their efficacy in children is crucial. This meta-analysis aimed to consolidate and quantify the evidence on the effectiveness of these core sun protection strategies in preventing pediatric sunburn. Methods: A systematic search of PubMed/MEDLINE, Scopus, Cochrane CENTRAL, and Web of Science (January 2014 - December 2024) identified randomized controlled trials and cohort studies evaluating sunscreen, protective apparel, or shade-seeking behaviors for sunburn prevention in individuals aged 0-18 years. Data on sunburn incidence were extracted, study quality assessed, and pooled Risk Ratios (RR) with 95% confidence intervals (CI) calculated using a random-effects model. Results: Six studies (two RCTs, four cohort studies) involving 8,500 children were included. Regular sunscreen use (SPF ≥30) significantly reduced sunburn incidence (RR 0.65, 95% CI 0.55-0.77). Protective apparel use also demonstrated substantial protection (RR 0.70, 95% CI 0.60-0.82). Enhanced shade provision and shade-seeking behaviors effectively lowered sunburn risk (RR 0.75, 95% CI 0.62-0.90). Multi-component strategies combining these approaches showed consistent protective benefits. Conclusion: This meta-analysis provides robust quantitative evidence that diligent sunscreen application, consistent use of protective apparel, and active shade-seeking are all significantly effective in reducing sunburn incidence in children. These findings strongly support multifaceted public health initiatives emphasizing comprehensive sun protection to safeguard pediatric skin health.

Optimizing Sunburn Prevention in Children: A Meta-Analysis of the Efficacy of Sunscreen Application, Protective Apparel, and Shade-Seeking Behaviors Ellistasari, Endra Yustin; Suci Widhiati; Sesia Pradestine
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 8 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i8.1355

Background: Childhood sunburn significantly elevates lifelong skin cancer risk, underscoring the need for effective prevention. While sunscreen, protective apparel, and shade-seeking are advocated, a quantitative synthesis of their efficacy in children is crucial. This meta-analysis aimed to consolidate and quantify the evidence on the effectiveness of these core sun protection strategies in preventing pediatric sunburn. Methods: A systematic search of PubMed/MEDLINE, Scopus, Cochrane CENTRAL, and Web of Science (January 2014 - December 2024) identified randomized controlled trials and cohort studies evaluating sunscreen, protective apparel, or shade-seeking behaviors for sunburn prevention in individuals aged 0-18 years. Data on sunburn incidence were extracted, study quality assessed, and pooled Risk Ratios (RR) with 95% confidence intervals (CI) calculated using a random-effects model. Results: Six studies (two RCTs, four cohort studies) involving 8,500 children were included. Regular sunscreen use (SPF ≥30) significantly reduced sunburn incidence (RR 0.65, 95% CI 0.55-0.77). Protective apparel use also demonstrated substantial protection (RR 0.70, 95% CI 0.60-0.82). Enhanced shade provision and shade-seeking behaviors effectively lowered sunburn risk (RR 0.75, 95% CI 0.62-0.90). Multi-component strategies combining these approaches showed consistent protective benefits. Conclusion: This meta-analysis provides robust quantitative evidence that diligent sunscreen application, consistent use of protective apparel, and active shade-seeking are all significantly effective in reducing sunburn incidence in children. These findings strongly support multifaceted public health initiatives emphasizing comprehensive sun protection to safeguard pediatric skin health.

Topical Polypeptide Gel in Diabetic Ulcers: Clinical Insights Into a New Adjunctive Treatment Modality Octarica, Stella Gracia; Widhiati, Suci; Ellistasari, Endra Yustin; Dharmawan, Nugrohoaji; Julianto, Indah
Journal of Social Research Vol. 4 No. 10 (2025): Journal of Social Research
Publisher : International Journal Labs

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55324/josr.v4i9.2794

Diabetic ulcers are a common and challenging complication of diabetes, contributing to morbidity, infection, and a high risk of amputation. Innovative adjuncts, such as topical polypeptide gel, which contains amino acids to support tissue regeneration, are proposed to accelerate wound healing; however, supporting clinical data are limited. A quasi-experimental analysis was conducted in 11 patients. Each received a daily topical 7% polypeptide gel in addition to standard care. Ulcer area was measured at baseline (day 0) and after 14 days using digital documentation analysis using ImageJ. The primary outcome was the percentage reduction in ulcer size. Patients were analysed for associations between percentage of improvement with age, gender, diabetes type, and ulcer site. Eleven patients with diabetic ulcers completed the 14-day treatment protocol. The cohort comprised 54.5% females with a mean age of 54.1 ± 7.3 years and predominantly insulin-dependent diabetes (72.7%). Baseline ulcer area averaged 982.1 ± 1315.1 mm², reducing to 184.0 ± 297.7 mm² by day 14, representing an 86.2 ± 6.7% mean area reduction. All patients achieved ?76% healing, with 45.5% achieving ?90% area reduction and 9.1% achieving complete healing. The Wilcoxon signed-rank test demonstrated statistically significant ulcer area reduction (Z = -2.934, p = 0.003). No significant associations were found between healing percentage and patient demographics, diabetes type, ulcer location, or comorbidity burden (all p > 0.05). No treatment-related adverse events were reported during the study period. Topical polypeptide gel demonstrated rapid wound surface area reduction in the majority of the subjects over 14 days, independent of patient age, gender, diabetes type, or ulcer location. These findings suggest its promise as an adjunctive early-phase therapy for diabetic ulcers.

Sikap dan Pemahaman Mahasiswa Kedokteran Semester Akhir Terhadap Laki Seks Lelaki sebagai Risiko Infeksi Menular Seksual dan HIV Hidayatullah, Lalu Muh Syahrul Rio; Kusumawardani, Arie; Ellistasari, Endra Yustin
Plexus Medical Journal Vol. 4 No. 4 (2025): Agustus
Publisher : Fakultas Kedokteran, Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/plexus.v4i4.2285

Latar Belakang: Penelitian ini bertujuan untuk mengeksplorasi sikap dan Tingkat pemahaman mahasiswa Kedokteran semester akhir (semester tujuh) mengenai Infeksi Menular Seksual (IMS) dan risiko Human Immunodeficiency Virus (HIV) di kalangan Lelaki Seks Lelaki (LSL). Meningkatnya prevalensi IMS dan HIV di kalangan LSL menunjukkan perlunya perhatian yang lebih besar terhadap pendidikan kesehatan dan kewaspadaan terhadap risiko infeksi pada kelompok ini. Sebagai calon tenaga kesehatan, mahasiswa Kedokteran memiliki peran penting dalam mencegah dan mengatasi masalah kesehatan ini. Metode: Penelitian ini menggunakan metode observasional analitik dengan desain cross-sectional. Populasi dalam penelitian ini adalah mahasiswa Kedokteran semester akhir Universitas Sebelas Maret, dengan jumlah sampel sebanyak 233 responden ditentukan menggunakan teknik total sampling . Teknik analisis data menggunakan analisis deskriptif dan analisis data kuantitatif. Hasil: Hasil penelitian menunjukan sikap mahasiswa semester akhir Kedokteran terhadap LSL sebagai faktor risiko IMS dan infeksi HIV sebagian besar mempunyai sikap sedang (ragu – ragu) sebanyak 93,1% terhadap topik penelitian. Tingkat pemahaman mahasiswa Kedokteran semester akhir terhadap LSL sebagai faktor risiko IMS kategori baik hanya sebanyak 42,1% dan pada infeksi HIV yang mempunyai pemahaman baik hanya sebanyak 44,6%, hasil ini masih kurang baik dan perlu ditingkatkan sebagai mahasiswa Kedokteran. Terdapat adanya hubungan yang signifikan antara tingkat pemahaman dengan sikap mahasiswa Kedokteran semester akhir dengan nilai signifikansi (IMS (p value = 0,014) & HIV (p value = 0,026)). Simpulan: Mahasiswa Kedokteran semester akhir memiliki tingkat pemahaman yang baik, sikap kategori yang tinggi dan terdapat hubungan antara tingkat pemahaman dan sikap terkait topik LSL sebagai faktor risiko IMS dan HIV.

Profile of Sexually Transmitted Diseases and The Influence of COVID-19 Pandemic on Sexual Transmitted Infection Testing in Dr. Moewardi General Hospital Ellistasari, Endra Yustin; Mawardi, Prasetyadi; Primisawitri, Pratiwi Prasetya; Octarica , Stella Gracia
Indonesian Basic and Experimental Health Sciences Vol. 13 No. 2 (2025): APRIL
Publisher : Rumah Sakit Umum Daerah Dr. Moewardi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/ibehs.vol13iss2pp34-40

Introduction Sexually transmitted infections (STIs) are diseases resulting from bacteria, viruses, or parasites transmitted during vaginal, anal, or oral sexual intercourse. The COVID-19 pandemic markedly influenced STI statistics, leading to potential underreporting of cases and heightened transmission rates. Objective : To analyse the profile of sexually transmitted infections and the influence of COVID-19 on STI assessments at the Dermatology and Venereology (DV) Outpatient Clinic of Dr. Moewardi General Hospital. Method A retrospective study using secondary data derived from medical records and registration logs at DV Outpatient Clinic of Dr. Moewardi General Hospital, spanning from January 2018 to December 2022. Results 284 new STI cases were documented in the before pandemic lockdown period, however during the pandemic lockdown, there was a significant decline of 149 new cases. After the pandemic lockdown, there was 200 additional cases of STIs. Up to 90 patients with STIs were co-infected with HIV. The predominant instance of STIs was condyloma acuminata., and there was a strong correlation between gender and the incidence of STIs (p=0.000; p<0.05) Conclusion There was a sharp decline in STI cases during the COVID-19 pandemic and a surge in STI cases after the COVID-19 pandemic, predominantly featuring instances of condyloma acuminata and syphilis.

VAGINOSIS BAKTERIALIS DENGAN KOINFEKSI KANDIDIASIS VULVOVAGINALIS Ellistasari, Endra Yustin; Widyastuti, Zilpa; Kasmitasari, Fitri; Wuri P, Adelia
Jurnal Cahaya Mandalika ISSN 2721-4796 (online) Vol. 4 No. 2 (2023)
Publisher : Institut Penelitian Dan Pengambangan Mandalika Indonesia (IP2MI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/jcm.v4i2.1509

Background : Bacterial vaginosis (BV) is an infection caused by an imbalance in the amount of normal vaginal flora and pathogenic bacteria. Vulvovaginal candidiasis (VVC) is an infection of candida in the vagina and vulva. Bacterial vaginosisis often accompanied by co-infection of VVC, it is caused by a reduction in the enzyme hydrogen peroxidase (H2O2) which can reduce the ability of leukocytes to fight infections, resulting in co-infection with other pathogens such as Candida sp. Factors that influence VB with co-infection of VVC include lack of hygiene, changing partners, the use of contraceptives in the uterus, the use of antiseptics with fragrances and other diseases such as diabetes mellitus. Case: A 40-year-old woman complaints of vaginal discharge since 3 months, white-gray vaginal discharge, fishy smell, no itching, and no pain during sexual intercourse. Physical examination revealed the presence of vulvar erythema, yellowish white discharge on the vaginal wall and portio cervicalis. Examination of 10% KOH showed the presence of pseudohyphae. Gram examination showed polymorphonuclear cells >50 per large field of view, gram negative rods 75-100 per large field of view, and clue cells >50%. The patient was given metronidazole 500 mg tablet twice a day for 14 days and fluconazole 150 mg single dose. Clinical and laboratory improvement was found on day 7 and recovered on day 14 after administration of therapy. Discussion: Bacterial vaginosis is an infection caused by the growth of anaerobic organisms that causes high morbidity due to their recurrence rate. This infection is often accompanied by a fungal infection such as kandidiasis vulvovaginalis caused by bad hygiene and increased humidity in genital area. The exact diagnosis of VB along with VVC is based on clinical symptoms, microscopic examination and culture. Antibiotics and antifungal drugs are the mainstay therapies for VB with co-infection of VVC.

Retinoblastoma tumor suppressor protein is not a risk factor for the location of condyloma acuminatum among adults Primisawitri, Pratiwi Prasetya; Mulianto, ⁠Nurrachmat; Ellistasari, Endra Yustin; Poncorini, Eti; Kariosentono, ⁠⁠Harijono; Widhiati, Suci; Wasita, Brian; Setyawan, Novan Adi; Yudhistira, Muhammad Yurizar
Universa Medicina Vol. 44 No. 3 (2025): Ahead Of Print
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2025.v44.343-349

BackgroundCondylomata acuminata (CA) are sexually transmitted infections primarily caused by low-risk HPV types 6 and 11. Retinoblastoma protein (pRb) interacts with HPV oncoproteins, leading to destabilization, genomic instability, and carcinogenesis. This study aimed to determine whether variations in pRb expression and sexual orientation are risk factors of the anatomical location of CA lesions and to compare differences in pRb expression by sexual orientation. MethodsA cross-sectional study was conducted among 33 patients aged >18 years with clinically and histopathologically confirmed CA. Demographic data, pRb expression assessed via excision biopsy and immunohistochemistry, and relevant risk factors were collected. Statistical analyses including Simple and multiple binary logistic regression were used to analyze the data, with model fit assessed using the Hosmer–Lemeshow test. ResultsWeak pRb expression predominated in anal lesions (92.3%; p=0.018) and among homosexual/bisexual individuals (57.9%; p=0.031), while moderate expression was more common in genital lesions (66.7%; p=0.018) and heterosexual individuals (57.1%; p=0.011). Simple logistic regression analysis showed that HIV status (OR=11.88; p=0.025), sexual orientation (OR=13.33; p=0.001), and moderate-to-strong pRb expression (OR=24.00; p=0.008; OR=12.00; p=0.048) were associated with lesion location. In the multivariate model, pRb expression was excluded due to multicollinearity. Sexual orientation remained the only independent predictor of lesion location (Adj OR=8.89; p=0.023), with heterosexual individuals more likely to present with genital lesions. ConclusionThe expression of pRb was not a risk factor of the anatomical location of CA, and differed between genital and anal CA. However, sexual orientation emerged as the dominant independent factor associated with lesion location.

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