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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) SAINSMAT bionature Jurnal Agroteknos BioWallacea Journal of Biological Research Jurnal Sains dan Inovasi Perikanan/Journal of Fishery and Innovation (JSIPi) Jurnal Sains dan Teknologi Pangan BIOMA : Jurnal Biologi Makassar Jurnal Biodjati Prosiding Seminar Nasional Riset Kuantitatif Terapan 2017 Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Jurnal MediLab Mandala Waluya Jurnal Abdimas Indonesia : Jurnal Abdimas Indonesia Jurnal Abdi Masyarakat Indonesia Jurnal Biofiskim: Pendidikan dan Pembelajaran IPA SAINSMAT: Jurnal Ilmiah Ilmu Pengetahuan Alam Jurnal Pengabdian Masyarakat Nasional Jurnal MediLab Mandala Waluya
Muzuni, Muzuni
Program Studi Bioteknologi FMIPA UHO, Kendari
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Isolasi dan Pengklonan Gen Penyandi H+-ATPase Membran Plasma dari Melastoma malabathricum L. ., Muzuni; Sopandie, Didy; Suharsono, Utut Widyastuti; ., Suharsono
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 1 (2014): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (737.981 KB) | DOI: 10.24831/jai.v42i1.8159

Abstract

ABSTRACT Melastoma malabathricum L. is an Al-accumulating plant that grows well in acidic soils with high level of soluble aluminum in the tropics. One of the important proteins in the detoxifying Al stress is a plasma membrane H+-ATPase, a most abundant protein on the plasma membrane, encoded by PMA gene. The objective of this research was to isolate and characterize the gene encoding plasma membrane H+-ATPase from M. malabathricum L. Full length cDNA of MmPMA had been successfully isolated through a gradual isolation of the gene. The 5’ end and middle part of the MmPMA gene had been successfully isolated by PCR by using total cDNA as template and pma primers designed from some plants, while the 3’ end of Mmpma had been isolated by 3’ RACE. The parts of the gene had been successfully joined by PCR. The joining product was successfully inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into E. coli DH5α. Nucleotide sequence analysis showed that the length of MmPMA coding sequence was 2,871 bp encoding 956 amino acids with molecular weight of 105.29 kDa and a predicted pI value of 6.84. Local alignment analysis based on nucleotide of mRNA showed that MmPMA is 82% identical to pma Vitis vinifera; 81% to pma Juglans regia, pma Populus trichocarpa, pma Sesbania rostrata, and pma Prunus persica and 80% to pma Lycopersicon esculentum. Based on deduced amino acid sequence, MmPMA is 94% identical to PMA Vitis vinifera and PMA Juglans regia; 93% to PMA Populus trichocarpa; 92% to PMA Vicia faba, Lycopersicon esculentum, and Arabidopsis thaliana, AHA4. MmPMA has 10 transmembrane domains, 4 cytoplasm loops, 6 functional domains and 3 autoregulatory domains.Keywords: aluminum, cDNA, MmPMA, PCR, RACE
PEMBERDAYAAN EKONOMI MASYARAKAT PESISIR MELALUI INOVASI PENGOLAHAN IKAN MENJADI NUGGET DAN SOSIS DI KELURAHAN BUNGKUTOKO Ahmad, Sitti Wirdhana; Ardiansyah, Ardiansyah; Amirullah, Amirullah; Ambardini, Sri; Muzuni, Muzuni; Ampa, Andi Tendri
Jurnal Pengabdian Masyarakat Nasional Vol 5, No 2 (2025)
Publisher : Universitas Mercu Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22441/pemanas.v5i2.36921

Abstract

Kegiatan demonstrasi produk olahan makanan berbasis sumber daya lokal dilaksanakan di Balai Kelurahan Bungkutoko, Kecamatan Nambo, Kota Kendari, dengan tujuan untuk memberdayakan ekonomi masyarakat pesisir. Dalam pelaksanaannya, mahasiswa yang didampingi oleh tim Dosen Pembimbing Lapangan (DPL) memperkenalkan dua jenis produk olahan ikan, yaitu sosis ikan dan nugget ikan. Metode yang digunakan dalam kegiatan ini adalah ceramah dan demonstrasi langsung pembuatan produk olahan ikan. Kegiatan diikuti oleh 37 peserta yang terdiri atas anggota UMKM, ibu-ibu PKK, dan tokoh masyarakat setempat. Selama pelatihan berlangsung, peserta menunjukkan antusiasme dan partisipasi aktif dalam setiap tahapan kegiatan. Melalui kegiatan ini, masyarakat memperoleh pengetahuan dan keterampilan praktis dalam mengolah hasil perikanan menjadi produk bernilai tambah yang berpotensi menjadi sumber penghasilan tambahan. Dampak nyata dari kegiatan ini terlihat pada meningkatnya motivasi peserta, khususnya ibu rumah tangga sebanyak 90% menyatakan kesiapan untuk memproduksi olahan ikan secara mandiri dari rumah, sehingga mendukung upaya penguatan ekonomi keluarga dan pemberdayaan masyarakat pesisir secara berkelanjutan.
Isolasi dan Pengklonan Fragmen cDNA Gen Penyandi H+-ATPase Membran Plasma dari Melastoma malabathricum L. , Muzuni; Didy Sopandie; Utut Widyastuti Suharsono; , Suharsono
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 1 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (378.981 KB) | DOI: 10.24831/jai.v38i1.1680

Abstract

Melastoma malabathricum L. grows well in acid soil with high level of soluble aluminum. One of the important proteins in the detoxifying acid and aluminum stress is a plasma membrane H+  -ATPase protein encoded by PMA gene. The objective of this research was to isolate and clone the cDNA fragment of MmPMA encoding plasma membrane H+ -ATPase from M. malabathricum L. By reverse transcription, total cDNA had been synthesized from the total RNA as template. The fragment of MmPMA  cDNA  had been successfully isolated by PCR by using total cDNA  as  template and PMA primer designed from conserved region for corresponding gene. This fragment had been successfully inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into E. coli DH5". Nucleotide sequence analysis showed that the length of MmPMA fragment is 806 bp encoding 268 amino acids. Local alignment analysis based on nucleotide of mRNA showed that MmPMA fragment was 81% identical to part of PMA of Sesbania rostrata, Juglans regia, and Prunus persica. Based on deduced amino acid sequence, MmPMA was 94% identical to part of PMA of Juglans regia; 93% to PMA of S. rostrata, and Arabidopsis thaliana. MmPMA fragment has phosphorylation intermediate domain (DKTGT) and ATP binding domain (KGAP, DPPR, MITGD, and GDGVN).   Keywords: isolation, Melastoma malabathricum L., MmPMA fragment, sequencing
Co-Authors , Suharsono . Jahidin . Jahidin, . Abe, Wa Ahmad Mustafa Ahmad, Sitti Wirdhana Alfin Alfin Alias, Alias Amin, Muhtar Amirullah -, Amirullah Ampa, Andi Tendri Andi Septiana Andriani, Rina Ansharullah ansharullah ARDIANSYAH ARDIANSYAH Aspadiah, Vica Asriwaty, Asriwaty Asrul Sani Azwar Syah, Muhamad Bafadal, Mentarry Balubi, Abdul Muis Barti, Barti Basrudin Basrudin Damhuri Damhuri Didy Sopandie DIRVAMENA BOER, DIRVAMENA Dwi Arinto Adi, Dwi Arinto Effendie, Muh Yamin Fahyuddin Fahyuddin, Fahyuddin Fristiohady, Adryan Hadijah Hadijah Haidin Haidin Halim Halim Halim Halim, Halim Hasani, La Ismaun Ismaun Jahidin Jahidin Jamaluddin Jamaluddin Jamili Jamili kaharudin, la ode Kasim, Ma'ruf La Maronta Galib La Maronta Galib, La Maronta MANGIRI, NINDY VANESSA Muhsin Muhsin Muhtar Amin Nur Arfa Yanti Nur Hikmah Nur Ramdani Nur Salam Nursalam Nursalam Nurwijayanti Puan Maharani Puspita Sari Ramadhani, Rizky Barkah Rinto Rinto Ruslin Hadanu Ruslin Hadanu Safilu Safilu Sahidin . Samsaifil Samsaifil Satriani Syarif Satriani Syarif, Satriani Solly Aryza Sri Ambardini Sri Wahyuni Suharsono . Suriana Suriana Tamrin Tamrin Taufik Walhidayah Trisandy, Darul Uhra Ali Utami, Andi Pratiwi Utut Widyastuti Suharsono Utut Widyastuti Suharsono Wa Ode Harlis Wardha Jalil Warhamni, Warhamni Yusnaini Yusnaini