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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) SAINSMAT bionature Jurnal Agroteknos BioWallacea Journal of Biological Research Jurnal Sains dan Inovasi Perikanan/Journal of Fishery and Innovation (JSIPi) Jurnal Sains dan Teknologi Pangan BIOMA : Jurnal Biologi Makassar Jurnal Biodjati ALCHEMY Jurnal Penelitian Kimia Prosiding Seminar Nasional Riset Kuantitatif Terapan 2017 Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Jurnal MediLab Mandala Waluya Jurnal Abdimas Indonesia : Jurnal Abdimas Indonesia Jurnal Abdi Masyarakat Indonesia Jurnal Biofiskim: Pendidikan dan Pembelajaran IPA SAINSMAT: Jurnal Ilmiah Ilmu Pengetahuan Alam Jurnal Pengabdian Masyarakat Nasional Jurnal Pusat Kolaborasi Pengabdian Kepada Masyarakat Jurnal MediLab Mandala Waluya

Muzuni, Muzuni

Program Studi Bioteknologi FMIPA UHO, Kendari

Author-ID : 213430

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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DETEKSI Mycobacterium tuberculosis PADA PASIEN SUSPEK TB DI PUSKESMAS POASIA BERDASARKAN EVALUASI PEWARNAAN DAN KEMUNCULANGEN IS6110 Satriani Syarif; Nur Ramdani; Muzuni
Jurnal MediLab Mandala Waluya Vol. 9 No. 1 (2025): JURNAL MEDILAB MANDALA WALUYA
Publisher : Prodi D4 Teknologi Laboratorium Medis, Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54883/medilab.v9i1.1229

Tuberkulosis (TB) adalah penyakit yang disebabkan oleh Mycobacterium tuberculosis. Penggunaan suspek TB sebagai responden karena kasus suspek TB memiliki jumlah yang cukup banyak di Puskesmas Poasia dengan data kasus 3 bulan terakhir yaitu, September sebanyak 242 orang, Oktober sebanyak 232 orang, dan November sebanyak 182 orang pada tahun 2022. Pemeriksaan mikroskopis dengan metode pewarnaan Ziehl-Neelsen masih menjadi pilihan pertama untuk deteksi awal TB. Kemunculan gen IS6110 menjadi penanda adanya Mycobacterium tuberculosis. Tujuan penelitian ini yaitu untuk mendeteksi Mycobacterium tuberculosis pada pasien suspek TB negatif di Puskesmas Poasia berdasarkan evaluasi pewarnaan dan berdasarkan kemunculan gen IS6110 Jenis penelitian ini adalah kualitatif, dengan desain non-experimental. Populasi pada penelitian ini adalah pasien suspek TB negatif rawat jalan yang melakukan pemeriksaan sputum di Puskesmas Poasia Kota Kendari. Pada penelitian ini sampel diambil dari 5 responden suspek TB negatif. Jenis sampel yang digunakan adalah sputum dan urine. Sampel sputum digunakan untuk pewarnaan Ziehl-Neelsen (ZN) dan sampel urine digunakan untuk pemeriksaan Polymerase Chain Reaction (PCR). Hasil pemeriksaan kelima sampel dengan kode 1A, 2A, 3A, 4A, dan 5A menunjukkan bahwa Mycobacterium tuberculosis tidak ditemukan pada sampel sputum suspek TB menggunakan metode pewarnaan Ziehl-Neelsen dan Mycobacterium tuberculosis tidak terdeteksi pada sampel urine suspek TB menggunakan metode PCR dengan amplifikasi yang dilakukan menggunakan primer Pt8 dan Pt9. Berdasarkan hasil penelitian dapat disimpulkan bahwa tidak terdeteksi Mycobacterium tuberculosis pada pasien suspek TB di Puskesmas Poasia berdasarkan evaluasi pewarnaan dan pada metode PCR. Untuk penelitian selanjutnya agar dilakukan deteksi Mycobacterium tuberculosis menggunakan metode PCR pada pasien yang baru terdiagnosa positif TB dan belum melakukan pengobatan.

Analysis of the Molecular Structure of Lipase-Dependent Chaperone from Ralstonia pickettii Strain BK6 Azwar Syah, Muhamad; Ambardini, Sri; Jamili, Jamili; Muzuni, Muzuni; Trisandy, Darul
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33770

Several biotechnology industries are exploring the characteristics of lipase-dependent chaperones due to their distinctive biochemical traits. This study aimed to employ bioinformatics to analyze the molecular structure of Ralstonia picketii BK6's lipase-dependent chaperon (LipRM). The sequence mapping and amino acid distribution were examined using BioEdit (version 7.0.9.1). SignalP 5.0 and Interpro are employed for signal peptide detection, whereas Swiss-Model and VMD 1.9.2 are used for molecular dynamics modelling. The results showed that the Shine-Dalgarno sequence was discovered in the LipRM promoter, seven nucleotides upstream of the initiation codon (AUG) with the 5'-AGGAGA-3', and has a terminator region that facilitates the formation of a secondary structure. The protein's 3D structure prediction results indicate differences in the alpha helix chains (residues 166-174 and 254-271) between LipRM and the reference lipase. LipRM's molecular structure comprises a detachable signal peptide, and with variations in helix alpha chain conformation and ligand geometry.

Isolasi dan Pengklonan Fragmen cDNA Gen Penyandi H+-ATPase Membran Plasma dari Melastoma malabathricum L. Muzuni, ,; Sopandie, Didy; Suharsono, Utut Widyastuti; Suharsono, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 1 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Melastoma malabathricum L. grows well in acid soil with high level of soluble aluminum. One of the important proteins in the detoxifying acid and aluminum stress is a plasma membrane H+ -ATPase protein encoded by PMA gene. The objective of this research was to isolate and clone the cDNA fragment of MmPMA encoding plasma membrane H+ -ATPase from M. malabathricum L. By reverse transcription, total cDNA had been synthesized from the total RNA as template. The fragment of MmPMA cDNA had been successfully isolated by PCR by using total cDNA as template and PMA primer designed from conserved region for corresponding gene. This fragment had been successfully inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into E. coli DH5". Nucleotide sequence analysis showed that the length of MmPMA fragment is 806 bp encoding 268 amino acids. Local alignment analysis based on nucleotide of mRNA showed that MmPMA fragment was 81% identical to part of PMA of Sesbania rostrata, Juglans regia, and Prunus persica. Based on deduced amino acid sequence, MmPMA was 94% identical to part of PMA of Juglans regia; 93% to PMA of S. rostrata, and Arabidopsis thaliana. MmPMA fragment has phosphorylation intermediate domain (DKTGT) and ATP binding domain (KGAP, DPPR, MITGD, and GDGVN). Keywords: isolation, Melastoma malabathricum L., MmPMA fragment, sequencing

RNAi dari Fragmen 3’UTR Gen Penyandi H+ -ATPase Membran Plasma Melastoma malabathricum L. dapat Menghambat Pertumbuhan Tanaman Tersebut Muzuni, ,; Sopandie, Didy; Suharsono, Utut Widyastuti; Suharsono, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 2 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The RNA silencing technique is an effective tool to examine the biological function of the target mRNA in plants. The recent development of GATEWAYTM cloning technology makes it easy to construct the RNAi vectors with trigger sequences and to analyze the function of a target gene. The objective of this research was to construct RNAi including the 3’UTR fragment of the gene coding plasma membrane H+-ATPase from Melastoma malabathricumL., 3’UTRMmpma. RNAi vector had been successfully constructed using GATEWAYTM cloning technology with the 3’UTRMmpma was used as double-stranded RNA (dsRNA) trigger sequence, pENTRTM/D-TOPO®as entry vector, and pANDA plasmid as destination vector. RNAi had been successfully introduced into M. malabathricumL. mediated by A. tumefaciensEHA101 to analyze the function of Mmpma gene in the detoxifying Al stress. Based on the test of transgenic plants tolerance to Al stress showed that in the nutrient solution including 3.2 mM Al (AlCl3.6H2O), the transgenic plants underwent growth suppression especially roots and leaves, whereas non-transgenic plants underwent growth normally. It showed that suppression of Mmpmagene expression by RNAi to M. malabathricumL. caused the plant became sensitive to Al.Keywords: 3’UTRMmpma, A. tumefaciens, Al stress, RNAi vector

Isolasi dan Pengklonan Gen Penyandi H+-ATPase Membran Plasma dari Melastoma malabathricum L. ., Muzuni; Sopandie, Didy; Suharsono, Utut Widyastuti; ., Suharsono
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 1 (2014): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACT Melastoma malabathricum L. is an Al-accumulating plant that grows well in acidic soils with high level of soluble aluminum in the tropics. One of the important proteins in the detoxifying Al stress is a plasma membrane H+-ATPase, a most abundant protein on the plasma membrane, encoded by PMA gene. The objective of this research was to isolate and characterize the gene encoding plasma membrane H+-ATPase from M. malabathricum L. Full length cDNA of MmPMA had been successfully isolated through a gradual isolation of the gene. The 5’ end and middle part of the MmPMA gene had been successfully isolated by PCR by using total cDNA as template and pma primers designed from some plants, while the 3’ end of Mmpma had been isolated by 3’ RACE. The parts of the gene had been successfully joined by PCR. The joining product was successfully inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into E. coli DH5α. Nucleotide sequence analysis showed that the length of MmPMA coding sequence was 2,871 bp encoding 956 amino acids with molecular weight of 105.29 kDa and a predicted pI value of 6.84. Local alignment analysis based on nucleotide of mRNA showed that MmPMA is 82% identical to pma Vitis vinifera; 81% to pma Juglans regia, pma Populus trichocarpa, pma Sesbania rostrata, and pma Prunus persica and 80% to pma Lycopersicon esculentum. Based on deduced amino acid sequence, MmPMA is 94% identical to PMA Vitis vinifera and PMA Juglans regia; 93% to PMA Populus trichocarpa; 92% to PMA Vicia faba, Lycopersicon esculentum, and Arabidopsis thaliana, AHA4. MmPMA has 10 transmembrane domains, 4 cytoplasm loops, 6 functional domains and 3 autoregulatory domains.Keywords: aluminum, cDNA, MmPMA, PCR, RACE

PEMBERDAYAAN EKONOMI MASYARAKAT PESISIR MELALUI INOVASI PENGOLAHAN IKAN MENJADI NUGGET DAN SOSIS DI KELURAHAN BUNGKUTOKO Ahmad, Sitti Wirdhana; Ardiansyah, Ardiansyah; Amirullah, Amirullah; Ambardini, Sri; Muzuni, Muzuni; Ampa, Andi Tendri
Jurnal Pengabdian Masyarakat Nasional Vol 5, No 2 (2025)
Publisher : Universitas Mercu Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22441/pemanas.v5i2.36921

Kegiatan demonstrasi produk olahan makanan berbasis sumber daya lokal dilaksanakan di Balai Kelurahan Bungkutoko, Kecamatan Nambo, Kota Kendari, dengan tujuan untuk memberdayakan ekonomi masyarakat pesisir. Dalam pelaksanaannya, mahasiswa yang didampingi oleh tim Dosen Pembimbing Lapangan (DPL) memperkenalkan dua jenis produk olahan ikan, yaitu sosis ikan dan nugget ikan. Metode yang digunakan dalam kegiatan ini adalah ceramah dan demonstrasi langsung pembuatan produk olahan ikan. Kegiatan diikuti oleh 37 peserta yang terdiri atas anggota UMKM, ibu-ibu PKK, dan tokoh masyarakat setempat. Selama pelatihan berlangsung, peserta menunjukkan antusiasme dan partisipasi aktif dalam setiap tahapan kegiatan. Melalui kegiatan ini, masyarakat memperoleh pengetahuan dan keterampilan praktis dalam mengolah hasil perikanan menjadi produk bernilai tambah yang berpotensi menjadi sumber penghasilan tambahan. Dampak nyata dari kegiatan ini terlihat pada meningkatnya motivasi peserta, khususnya ibu rumah tangga sebanyak 90% menyatakan kesiapan untuk memproduksi olahan ikan secara mandiri dari rumah, sehingga mendukung upaya penguatan ekonomi keluarga dan pemberdayaan masyarakat pesisir secara berkelanjutan.

Co-Authors , Suharsono . Jahidin . Jahidin, . Abe, Wa Ahmad Mustafa Ahmad, Sitti Wirdhana Alfin Alfin Alias, Alias Amin, Muhtar Amirullah -, Amirullah Ampa, Andi Tendri Andi Septiana Andriani, Rina Ansharullah ansharullah ARDIANSYAH ARDIANSYAH Ardiansyah Ardiansyah Aspadiah, Vica Asriwaty, Asriwaty Asrul Sani Azwar Syah, Muhamad Bafadal, Mentarry Balubi, Abdul Muis Barti, Barti Basrudin Basrudin Damhuri Damhuri Didy Sopandie DIRVAMENA BOER, DIRVAMENA Dwi Arinto Adi, Dwi Arinto Effendie, Muh Yamin Fahyuddin Fahyuddin, Fahyuddin Fristiohady, Adryan Hadijah Hadijah Haidin Haidin Halim Halim Halim Halim, Halim Hasani, La Ismaun Ismaun Jahidin Jahidin Jamaluddin Jamaluddin Jamaluddin Jamaluddin Jamili Jamili kaharudin, la ode Kasim, Ma'ruf La Maronta Galib La Maronta Galib, La Maronta MANGIRI, NINDY VANESSA Muhsin Muhsin Muhtar Amin Nur Arfa Yanti Nur Hikmah Nur Ramdani Nur Salam Nursalam Nursalam Nurwijayanti Prasetya, Wandy Murti Puan Maharani Puspita Sari Ramadhani, Rizky Barkah Rinto Rinto Ruslin Hadanu Ruslin Hadanu Safilu Safilu Sahidin . Samsaifil Samsaifil Satriani Syarif Satriani Syarif, Satriani Solly Aryza Sri Ambardini Sri Wahyuni Suharsono . Suriana Suriana Suriana Suriana Tamrin Tamrin Taufik Walhidayah Trisandy, Darul Uhra Ali Utami, Andi Pratiwi Utut Widyastuti Suharsono Wa Ode Harlis Wardha Jalil Warhamni, Warhamni Yusnaini Yusnaini

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