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Improving Science Learning Activities and Outcomes by Using Problem Based Learning Model at Elementary School Saleh, Ahmad; Ahda, Yuni; Fitria, Yanti
Jurnal Basicedu Vol. 4 No. 4 (2020)
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/basicedu.v4i4.578

Abstract

Science learning requires direct experience in order to develop the ability to explore and understand the natural surroundings scientifically. So far in elementary school at Bukittinggi, the process of learning science only memorizes facts, principles or theories. In science learning, students are not involved in finding or applying their own ideas and they do not instill the values contained in the learning. The science learning process in classroom is not yet fully in accordance with the demands of the curriculum. The teacher provides more material in the textbook without relating it to students' daily problems that are in accordance with the material, thus causing less interesting in learning. The teacher also has not actively involved all students in learning and emphasizes learning only on students who are smarter. The purpose of this study was to increase the activity and learning outcomes of science learning by using a problem-based learning model in SDN 11 Aur Kuning Bukittinggi, West Sumatra. This classroom action research was conducted in 2 cycles with the research subjects of grade IV students with totalling 25 people. The research data were collected through observation and tests. The effectiveness of actions in each cycle from the results of observations and tests is described and then reflected to take corrective action in the next cycle. In science learning, students' learning activities were observed, namely: visual activities, oral activities, writing activities, motoric activities and emotional activities. The research findings show that the problem-based learning model can increase student activity and learning outcomes.
VALIDITY OF SCIENCE PROCESS SKILLS TEST INSTRUMENT FOR PROSPECTIVE MI/SD TEACHER STUDENTS IN SCIENCE LEARNING Syafrilianto, Syafrilianto; Afriandi, Muhammad; Festiyed, Festiyed; Yerimadesi, Yerimadesi; Ahda, Yuni; Alberida, Heffi
FORUM PAEDAGOGIK Vol 16, No 1 (2025)
Publisher : Universitas Islam Negeri Syekh Ali Hasan Ahmad Addary Padangsidimpuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24952/paedagogik.v16i1.15119

Abstract

Validity refers to the degree or level of accuracy of a research instrument used to measure a variable and serves as an essential component in the development of instruments. This study aims to determine the construct and content validity of the science process skills (SPS) test instrument for the Science Learning course in the MI/SD Teacher Education Program at the State Islamic University of Sheikh Ali Hasan Ahmad Addary, Padangsidimpuan. The research method employed is a descriptive quantitative-qualitative approach, with data collected using observation sheets for the assessment of the SPS test instrument by two validators. The data from the validators' assessments were analyzed using Gregory's Test formula to determine the instrument's validity level. The results show that 13 SPS test items received validator assessments in the range of scores 3-4, categorized as highly relevant, with an overall validity score of 1 (one). Therefore, it can be concluded that the 13 test items of the science process skills instrument on the topic of temperature and heat possess a very high level of validity.
Primer Design of EDNRB Gene Exon 5 and PCR Optimization for Gene Mutation Analysis in Hirschsprung's Disease Anggun, Septiasri; Ahda, Yuni
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8971

Abstract

HD disease is a common cause of neonatal intestinal obstruction. HD disease is a congenital disorder of the colon characterized by the absence of parasympathetic ganglion cells in the sub mucosal plexus of Meissneri and the mienterikus plexus of Auerbachi. Objective: To design specific primers for amplification of exon 5 in EDNRB gene and test the specificity in silico. To determine the optimum PCR conditions for amplification of exon 5 in EDNRB gene. Methods This type of research is descriptive. The purpose of descriptive research is to provide an overview or description of the EDNRB exon 5 gene primer design and PCR optimization. Electrophoresis results showed the temperature of 56 oC is the right temperature characterized by a single band and looks the thickest and brightest with the appropriate amplicons size of 367 bp, The results for the EDNRB gene primer exon 5 obtained the optimum concentration of 0.8 µM. This is characterized by DNA bands that look clear and thickest. The specific sequence of forward primer is 5'ATTTCA GAGACG GGAAG TGG3' reverse primer is 5'GGCACT CACTCATCATCTTG 3' which produces amplicons with a length of 367 bp. The optimum annealing temperature is 56 oC and the optimum primer concentration is 0.8 µM.  
DESAIN PRIMER GEN EDNRB EKSON 4 DAN OPTIMASI PCR UNTUK ANALISIS MUTASI GEN PADA HIRSCHSPRUNG DISEASE Hidayah, Nurul; Ahda, Yuni; Achyar, Afifatul; Atifah, Yusni
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5954

Abstract

EDNRB gene is a gene located on chromosome 13q22, plays a role in the signaling pathway during the development of the ENS intestinal nerves. Mutations in the EDNRB gene in humans are associated with Hirschsprung Disease (HD). Mutations in the EDNRB gene are the second most common cause after the RET gene in cases of HD with a percentage of 5–10%. To detect mutations, PCR products are needed. Primers are one of the basic components of PCR, while optimization of annealing temperature and optimization of primer concentration are factors in the success of primer attachment to the target gene. This study aims to design EDNRB exon 4 gene primers and determine the optimum annealing temperature and primer concentration to amplify the EDNRB gene. Primer design with Geneious Prime, and good primer criteria are analyzed in silico. Optimization of annealing temperature and primer concentration is carried out with gradient PCR and variations in primer concentration. The results of this study obtained the best primers, namely the forward primer 5'- CAGTAAGTGTGGCCTGAAAG-3' and the reverse primer 5'-GTGGAACCGAAGTGACTAGA-3', which amplified the EDNRB gene exon 4. The optimum PCR conditions for amplification of exon 4 of the EDNRB gene were at an annealing temperature of 57.2 ℃ and at a primer concentration of 0.5 μM.
DESAIN PRIMER GEN EDNRB EKSON 1 UNTUK ANALISIS MUTASI GEN PADA HIRSCHSPRUNG’S DISEASE Wahyuni, Nur Anisa; Yuni Ahda
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 10 No. 2 (2025): Bioma : Juli - Desember 2025
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The endothelin receptor type B (EDNRB) gene is located on chromosome 13, consisting of 7 exons and 6 introns. The EDNRB protein produced from this gene belongs to the family of G-protein coupled receptors. Mutations in the EDNRB gene play a role in some cases of Hirschsprung's cause, although the frequency is low. This study aims to design primers and test specificity using BLAST primers to determine the specific primer attached to the EDNRB gene. Primers were designed using Geneious Prime, which were then analyzed in silico based on good primer criteria. The results of this research showed that primers made using Geneious Prime produced the best primers, namely forward primer 5'-GAGTTCGGAGCTTTGCCT-3' and reverse 5'-AGCCCACCA TGATTTCA GC-3' and the results of the specificity test showed that the primer specifically attached to chromosome 13 which is the location of the EDNRB gene (13q22) with a product length of 879 bp. Kata kunci : EDNRB Gene, Primer Design, Hirschsprung’s Disease
Polyploid Induction of Long Bean (Vigna sinensis) Using Colchicine with Different Concentrations Fitri, Anisa; Afandi, Echa Azkia; Anggun, Septiasri; Yuni Ahda
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9063

Abstract

Long bean (Vigna sinensis) is a plant from the Leguminosae family, which has an important role in the Indonesian economy because it is widely consumed as food. The purpose of this research is to utilize mutation technology in supporting plant breeding to produce superior long bean varieties. The research method carried out was a non-factorial Randomized Group Design (RAK), namely soaking the seeds of long bean plants with colchicine to see the effect of colchicine treatment on chromosomal mutations in long bean plants. The results showed significant differences between colchicine concentrations of 0.02% and 0.03%. At a concentration of 0.02%, the cell nucleus appeared solid red and in the center of the cell, but the number and shape of chromosomes were not clearly visible. This concentration is not optimal for inducing polyploidy. Meanwhile, at a concentration of 0.03%, the chromosomes were more clearly visible than the previous concentration.
Practicality of E-Module Teaching Materials Integrated With Islamic Values On Global Warming Material at Senior High School Agusminarti, Agusminarti; Sholihat, Neng; Nurbaiti, Desy; Ahda, Yuni
Biosfer: Jurnal Tadris Biologi Vol 15 No 1 (2024): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v15i1.18561

Abstract

Islamic values in the subject matter of global warming symptoms for class XI at Senior high school. This research is a continuation of R&D research and development with the ADDIE model using 5 stages namely Analysis, Design, Development, Implementation, and Evaluation. The research instrument used was a practicality test questionnaire and student answer sheets on the e-module. there are 31 students and one educator have participated in this practicality test. The results of data analysis on the average value at the practicality test stage were carried out by educators and students. With the very practical category, the practicality analysis value of educators is 92.65 %, Very practical criteria for data analysis by students yielded 89.09 %. The results of the study concluded that the integrated e-module of Islamic values in the material on global warming symptoms that was developed was very practical to use in the learning process for class XI as an alternative teaching material in the learning processABSTRAK: Penelitian ini bertujuan untuk mengukur tingkat praktikalitas e-modul terintegrasi nilai-nilai islam pada materi gejala pemanasan global untuk kelas XI Sekolah Menegah Atas. Penelitian ini merupakan lanjutan penelitian dan pengembangan R&D dengan model ADDIE menggunakan 5 tahapan yaitu Analisis, Desain, Pengembangan, Implementasi, dan Evaluasi. Instrumen penelitian yang digunakan berupa angket uji praktikalitas dan lembar jawaban peserta didik pada e-modul. terdapat 31 peserta didik dan satu pendidik telah berpartisipasi dalam pengujian kepraktisan ini. Hasil analisis data rata-rata nilai pada tahap uji praktikalitas dilakukan oleh pendidik dan peserta didik. Dengan katerogi sangat praktis diperoleh nilai analisis kepraktisan pendidik sebesar 92,65%, Kriteria sangat praktis analisis data oleh peserta didik menghasilkan sebesar 89,09%,. Hasil penelitian menyimpulkan bahwa e-modul terintegrasi nilai-nilai islam pada materi gejala pemanasan global yang dikembangkan sangat praktis digunakan dalam proses pembelajaran kelas XI sebagai bahan ajar alternative dalam proses pembelajaran.
STEM CELL THERAPY IN STROKE Zalni, Tita Yunia; Ahda, Yuni; Razak, Abdul
Journal of Stem Cell Research and Tissue Engineering Vol. 7 No. 1 (2023): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v7i1.36443

Abstract

Stem cells are emerging as an attractive option because of their intrinsic ability as vectors for drug delivery to diseased tissues. The term stem cells or stem cells was first introduced by a Russian histologist, Alexander Maksimov (1874-1928). The purpose of this study is to describe the research and development of stem cell applications for stroke. This study is a literature study (review article) regarding stem cells (stem cells) in stroke which has been discussed in several scientific journals. From several articles that have been collected in giving stem cells to people with stroke, namely in bone marrow. MSC (Mesenchymal Stem Cell) is a multipotent stem cell from bone marrow and Adult SPM and because of its high applicability. Bone marrow is a rich source of hematopoietic stem cells so it can be applied to stroke patients.
DESAIN PRIMER GEN EDNRB EKSON 6 DAN OPTIMASI PCR UNTUK ANALISIS MUTASI GEN PADA HIRSCHSPRUNG’S DISEASE Yuda Meisya, Divia; Ahda, Yuni
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Hirschsprung’s Disease (HSCR) is a congenital disorder characterized by the absence of ganglion cells in the intestinal wall, leading to impaired intestinal motility. One of the key genes involved in the pathogenesis of HSCR is the Endothelin Receptor Type B (EDNRB) gene, particularly mutations in exon 6. Detecting these mutations requires specific primers and well-optimized PCR conditions. This study aims to design specific primers for exon 6 of the EDNRB gene using in silico methods and to determine optimal PCR conditions through annealing temperature and primer concentration optimization. Primers were designed based on the EDNRB gene sequence (Accession No. NG_011630.3) using Geneious Prime and validated for specificity using Primer-BLAST. PCR optimization was conducted in vitro using various annealing temperatures 52.8–55.3°C and primer concentrations 0.5–1.1 µM on normal human DNA samples. PCR products were analyzed by 1.5% agarose gel electrophoresis. The primer design for exon 6 of the EDNRB gene yielded the following results: both forward and reverse primers were 20 bp in length, with melting temperatures (Tm) of 58.1°C and 56.1°C, and GC contents of 55% and 50%, respectively. In silico analysis confirmed primer specificity to chromosome 13. Optimal PCR conditions were achieved at an annealing temperature of 55.3°C and a primer concentration of 0.7 µM, yielding a single amplicon of 517 bp.
Pengaruh Teratogen AlCl3 (Aluminium chloride) terhadap Perkembangan Embrio Ayam (Gallus gallus domesticus) Najib Rahman. G; Azeli, Saskia Putri; Hayu, Elvira; Ahda, Yuni; Atifah, Yusni
JURNAL BIOSENSE Vol 6 No 02 (2023): Edisi Desember 2023
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v6i02.3364

Abstract

Teratogen berefek membahayakan terhadap perkembangan embrio. Tujuan penelitian ini untuk mengetahui pengaruh teratogen AlCl3 terhadap perkembangan embrio ayam (Gallus gallus domesticus). Metode penelitian ini dilakukan secara eksperimental dengan perlakuan induksi AlCl3 0,2 mg/L dan kontrol (tanpa perlakuan). Pemberian perlakuan diberikan kepada masing-masing 5 butir telur ayam yang kemudian diinkubasi selama 48 jam. Pengamatan preparat embrio ayam menggunakan whole mount method dengan pewarnaan metilen blue dan eosin. Hasil penelitian menunjukkan ukuran embrio pada kelompok perlakuan lebih kecil (0,6 cm) dibandingkan dengan embrio kontrol (1cm). Jumlah somit pada kelompok perlakuan sejumlah 12 pasang sedangkan pada kelompok kontrol 28 pasang somit. Ukuran kepala lebih kecil jika dibandingkan dengan kelompok kontrol. Penelitian ini menunjukkan bahwa AlCl3 dapat menyebabkan malformasi pada embrio ayam, terkait dengan ukuran embrio, jumlah somit, dan ukuran kepala.
Co-Authors Abdul Rasak Abdul Razak Abdul Razak Achyar, Afifatul Afandi, Echa Azkia Afriandi, Muhammad Agusminarti Ahmad Fauzan Ahmad Saleh Akbar, Imam Qodri Ambiyar, Ambiyar Ananda, Azwir Anggun, Septiasri Anisha S Atifah, Yusni Atmazaki Atmazaki Ayesa, Putri Ayu, Duni Azalia, Salsa Novela Azeli, Saskia Putri Azwir Anhar Azzahra, Yolanda Belia Putri BR Tarigan, Siti Nadiah Zahra D. N. Putri, Dini Daniel Happy Putra Darussyamsu, Rahmawati Dezi Handayani Diana Susanti Dualom, Siti Dwi Hilda Putri Efdillah, Syilvia Eriza, Deni Septia Evi Suryawati Fachry Abda El Rahman Fadhil Ramadhan, Bintang FAJRINA, SUCI Ferdyan, Rhavy Festiyed Fidela, Wita Fitri Arsih Fitri, Anisa Fitri, Lel Fitria, Davina Fitria, Yanti Gunaria Siagian Haryanto Haryanto Hastutin, Sri Yuni Hayu, Elvira Heffi Alberida Indra Hartanto Iqra, Kuntum Nurul Isnaini Isnaini Jörg Gromoll K. Sari, Jelly Keiko Kasy Billah Keiko Kasy Billah Linda Wati Lufri Lufri Marcloryne Agnesia Masyitoh Masyitoh Maulina, Adinda Rizky Meisya, Divia Yuda Miftahul Jannah Monica, Della Trya Muhammad Ikbal Mutia Dwi Herakasih Mutia, Ravena Najib Rahman. G Neng Sholihat Nining Nuraida Nukman Moeloek Nurbaiti, Desy NURUL HIDAYAH P. Ningky, Yiyin Purnomo Soeharso Rahma Amelia, Anisha Chahya Rahmadhani Fitri Rahmawati Darussyamsu Ramadhan Sumarmin Ratna Nila Sari Regina Revi Gina Gunawan Rinti Mutiara Sari Samsudin, Syakirah Septelia I. Wanandi Siregar, Titik Ermila Sri Suciati Ningsih SUCI FAJRINA Syafrilianto, Syafrilianto Syari, Elsy Melia T. Berlian Wahyuni, Nur Anisa Winda Ayu Fietri Yerimades Yerimades Yerimadesi Yuda Meisya, Divia Yusi Febriani Zalni, Tita Yunia Zega, Dwi Junita Zhafira