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All Journal Biospecies Kolaboratif Bioeducation Journal Penelitian Pendidikan Biosfer: Jurnal Tadris Biologi BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan QUANTUM: Jurnal Inovasi Pendidikan Sains BIOMA : Jurnal Biologi Makassar Jurnal Ilmiah Pendidikan Fisika Jurnal Biologi Tropis Jurnal Basicedu Jurnal Ilmu Kedokteran dan Kesehatan Biota Atrium Pendidikan Biologi Serambi Biologi Journal of Stem Cell Research and Tissue Engineering Sainstek : Jurnal Sains dan Teknologi Jurnal Biogenerasi Jurnal Ekologi, Masyarakat dan Sains FORUM PAEDAGOGIK Jurnal Biosense BiosciED: Journal of Biological Science and Education TROPICAL GENETICS Jurnal Basicedu Tropical Genetics Jurnal Serambi Biologi Jurnal Pendidikan IPA Indonesia
Yuni Ahda
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Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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MOTILITAS DAN VIABILITAS SPERMATOZOA MENCIT JANTAN (Mus musculus L.) SETELAH PEMBERIAN EKSTRAK DAUN KUMIS KUCING ( Orthosiphon aristatus) Ayesa, Putri; Fadhil Ramadhan, Bintang; Fitria, Davina; Mutia, Ravena; Ahda, Yuni; Atifah, Yusni
Jurnal Biogenerasi Vol. 10 No. 3 (2025): Volume 10 no 3 tahun 2025 terbit september
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i3.6781

Abstract

This study aims to evaluate the effect of cat whisker leaf extract (Orthosiphon aristatus) on motility and viability of male mice spermatozoa (Mus musculus L.). The research method used was experimental. This study was conducted to determine the effect of the treatment given to a matter under study. Consists of three treatments, namely control (without treatment), treatment dose 0.5 mg and treatment dose 0.8 mg Cat whisker leaf extract is given orally for 15 days. After treatment, spermatozoa were taken from the epididymis of male mice and tested for motility and viability using statistical analysis through the One-Way Anova test. The results showed that the decrease in motility and viability of spermatozoa depended on the dose given. The higher the dose of cat whisker extract, the motility and viability of spermatozoa decreased. In mice with a dose of 0.8 mg spermatozoan motility is silent and does not move and for viability it dies. The control treatment showed different motility but remained alive with good viability. Meanwhile, the treatment group showed a decrease in motility and viability, with control mice.
Effect of Ethanolic Extract of Avocado (Persea americana Mill.) Seed on the Testis Histology (Mus musculus l.) Induced Monosodium Glutamate (MSG) Atifah, Yusni; Zega, Dwi Junita; Ahda, Yuni; Farma, Siska Alicia
Biotik Vol 12 No 2 (2024): JURNAL BIOTIK
Publisher : Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/biotik.v12i2.24419

Abstract

Monosodium Glutamate (MSG) is a substance that is able to produce free radicals such as ROS (Reactive Oxygen Species), which can cause oxidative stress in the body and damage reproductive organs such as the testes. The purpose of this study was to determine the effect of avocado seed ethanol extract (Persea americana Mill.) on the histology of the testes of mice (Mus musculus L.) induced monosodium glutamate (MSG). This research is an experimental study that was conducted in October 2023 – January 2024 at Biology Laboratory, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang. The design used is a Complete Randomized Design (CRD) with 4 treatments and 6 repeats. The data obtained were analyzed using the ANOVA test (Analysis of variance) using the SPSS 23 application. The results showed that the administration of avocado seed ethanol extract showed a noticeable effect on the diameter of the testes of male mice exposed to MSG. However, it showed no noticeable influence on testicular weight, seminiferous tubule diameter and Leydig cell count. Ethanol extract avocado seed dose 0.84 mg /g body weight / day (P1) was able to improve the diameter of mice testes that had been induced MSGKeyword: Avocado seed extract, Histology of testis, Mice, MSG
Literature Review: The Role of RET Gene Mutations in The Emergence of Hirschsprung Disease Azalia, Salsa Novela; Ahda, Yuni
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6740

Abstract

Hirschsprung’s disease is a congenital condition characterized by a failure of nerve function along the gastrointestinal (GI) tract, resulting in the absence of enteric ganglia in the most distal segment of the colon. Although the prevalence rate is 1:5000 babies born, Hirschsprung’s disease can have a significant impact on patients, especially in terms of quality of life. One of the main genetic factors often associated with the etiopathogenesis of Hirschsprung’s disease is the RET gene. This study aims to provide scientific insight into advances in the understanding of genetic variants associated with RET gene mutations and their role in causing Hirschsprung disease. The research design used was literature review, articles were collected based on sources derived from Google Scholar, PubMed, and EMBASE which included national and international journals published in the last 10 years. Based on the articles collected, it was found that the RET gene is essential for the growth, migration, and maturation of precursor cells into functional neurons. Mutations in the RET gene can interfere with the normal formation and function of nerve cells, leading to symptoms typical of Hirschsprung’s disease.
Pemanfaatan Kotoran Sapi Menjadi Biogas Sebagai Upaya Pengendalian Limbah Peternakan Fidela, Wita; Ahda, Yuni; Zhafira; Febriani, Yusi; Azzahra, Yolanda; P. Ningky, Yiyin; T. Berlian; Regina; K. Sari, Jelly; Ayu, Duni; D. N. Putri, Dini; Fajrina, Suci
Jurnal Ekologi, Masyarakat dan Sains Vol 5 No 2 (2024): Jul-Des 2024
Publisher : ECOTAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55448/0br55f55

Abstract

Cows are mammals that are widely bred throughout Indonesia. The large number of cattle breeders in Indonesia has positive and negative sides when viewed from various points of view. Cattle farming generally produces livestock waste, especially manure. Cow dung can be used as a basic ingredient for alternative energy production in the form of biogas. However, in reality, many cattle breeders ignore the problem of animal waste and it is wasted, even causing pollution to the environment. This research aims to test the use of cow dung as the main organic material in biogas production as a sustainable effort to minimize the environmental impact due to livestock waste and as an alternative energy. This research uses an experimental method with a biogas production trial for 21 days using a simple digester. The research results show that cow dung has great potential as a raw material for making biogas. Other factors influence biogas production, such as acidity levels, environmental temperature, and manure conditions.
The Effectiveness of Project-Based Learning Model with RAHMA Syntax to Improve Prospective Biology Teachers’ Critical Thinking Skills Darussyamsu, Rahmawati; Lufri, Lufri; Ahda, Yuni; Alberida, Heffi; Ambiyar, Ambiyar; Samsudin, Syakirah
Jurnal Pendidikan IPA Indonesia Vol. 13 No. 4 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/xy8mr440

Abstract

This research aims to measure the effectiveness of the Project-Based Learning Model with RAHMA Syntax (PjBL-RAHMA) in improving prospective biology teachers’ critical thinking skills. RAHMA is an acronym for Recognizing Problems, Analyzing Problems, Handling Projects, Monitoring Progress, and Assessing Results. This research was quasi-experimental with a posttest-only control group design. This research involved 223 prospective biology teachers from three well-accredited public universities in Sumatra, Indonesia. Data from the critical thinking skills test results were analyzed using JASP software. Prospective biology teachers’ achievement of critical thinking skills after learning using the PjBL-RAHMA model showed that the experimental class was better than the control class. This research concludes that PjBL-RAHMA effectively improves prospective biology teachers’ critical thinking skills. This research also explains the contribution of each PjBL-RAHMA syntax in improving prospective biology teachers’ critical thinking skills. The PjBL-RAHMA syntax has increased prospective biology teachers’ ability to understand and analyze real-life problems, providing good reasons, logical thinking, and creating solutions to problems, especially in pedagogy.
PENGEMBANGAN INSTRUMEN TES BERBASIS KEMAMPUAN BERPIKIR KRITIS BERLANDASKAN LITERASI SAINS PADA MATERI PENCEMARAN LINGKUNGAN Gunaria Siagian; Revi Gina Gunawan; Festiyed Festiyed; Yuni Ahda; Yerimades Yerimades
QUANTUM: Jurnal Inovasi Pendidikan Sains Vol 14, No 2 (2023): Oktober 2023
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/quantum.v14i2.15319

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui kelayakan instrumen tes berbasis kemampuan berpikir kritis berlandaskan literasi sains pada materi pencemaran lingkungan dengan melihat aspek validitas, reliabilitas, tingkat kesukaran, daya beda, dan uji respon pada mahasiswa.  Penelitian ini merupakan penelitian pengembangan dengan Analysis, Design, Development, Implementatiom, and Evaluation (ADDIE). Data penelitian ini diperoleh dengan mengujicobakan instrumen tes biologi yang dikembangkan berlandaskan literasi sains berupa tes essay sebanyak 20 soal. Analisis validasi isi menunjukkan bahwa sebanyak 17 soal valid. Reliabilitas instrumen 0,78, tingkat kesukaran 0,30-0,72 dan daya beda 0,04-0,72. Hasil uji respon diketahui 75% responden yang menyatakan sangat setuju dengan instrumen tes jenis ini. Berdasarkan kriteria tersebut, maka 14 soal telah dinyatakan baik dan layak digunakan sebagai instrumen tes biologi untuk melatih keterampilan berpikir kritis bagi mahasiswa. Intrumen tes yang dikembangkan telah masuk dalam kategori layak atau baik digunakan sebagai instrumen tes yang dapat melatih keterampilan berpikir kritis. Intrumen tes ini telah memenuhi kriteria validitas ahli dengan presentase 85% soal valid dan 15% soal tidak valid, reliabilitas 0,78 pada kategori tinggi, tingkat kesukaran 0,30 sampai 0,72, dan daya beda 0,04 sampai 0,72. Intrumen tes yang dikembangkan juga telah mendapat respon dengan presentase 75% pada kategori sangat baik dari responden.
Validity of Assessment Instruments for Evaluating the Feasibility of Developing an Integrated STEM Inquiry Model in 21st-Century Education Isnaini Isnaini; Atmazaki Atmazaki; Yuni Ahda; Suci Fajrina
Jurnal Ilmiah Pendidikan Fisika Vol 9, No 2 (2025): JUNE 2025
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jipf.v9i2.15396

Abstract

The validity of assessment instruments is a crucial element in educational research, particularly in evaluating the success of a learning model. In developing an integrated STEM inquiry learning model, it is necessary first to validate the assessment instruments to ensure they can effectively measure the desired learning outcomes, including both academic knowledge and the 21st-century skills developed by students. This study evaluates the validity of the instruments used to assess the feasibility of the integrated STEM inquiry model, which includes the model book, teacher's book, student's book, and a 21st-century skills assessment instrument. The instruments were validated through expert judgment involving three validators: an educational expert in evaluation, a STEM curriculum developer, and a language and communication practitioner. The instruments were assessed using a five-point Likert scale, and the data were analyzed using descriptive statistics to determine content, construct, and criterion validity. The results show that the 21st-century skills instrument scored 0.89, the model book scored 0.84, the student book scored 0.87, and the teacher book scored 0.86, all falling into the very high validity category. These findings indicate that the instruments are appropriate and reliable for measuring both academic competencies and essential 21st-century skills such as problem-solving, creativity, and collaboration. The study concludes that employing valid assessment instruments is essential for the effective implementation of the integrated STEM inquiry model and for ensuring its success in fostering the holistic development of students. The findings also highlight the importance of instrument validation in educational settings to ensure that learning models achieve their intended outcomes and prepare students for future challenges.
Genotyping of Sumatera local variety of citrus using random amplified polymorphism DNA (RAPD) technique Rinti Mutiara Sari; Afifatul A Achyar; Yuni Ahda; Dwi Hilda Putri
Tropical Genetics Vol. 2 No. 2 (2022):
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v2i2.29

Abstract

Indonesia has local varieties of citrus that are no less than imported citrus, especially in terms of fruit freshness. However, people are more interested in the color of citrus peel so people prefer imported citrus to local citrus, especially in Sumatera. Therefore, it is necessary to carry out efforts to conserve and improve the characteristics of these citrus to improve their quality through plant breeding. This study aims to optimize DNA isolation methods for citrus fruit samples with Chelex-TE and to determine the genetic profile of local Sumatera citrus and imported citrus using the genotyping RAPD. The samples used were several local citrus in Sumatera (Citrus Siam Mountain Omeh, Citrus Madu, Citrus Keprok Maga, Citrus Keprok Brastepu and Citrus Pasaman) and imported Citrus (Citrus Sunkist, Citrus Clemengold, Citrus Murkot and Citrus Wokam). DNA was isolated using the 10% Chelex-TE method which was optimized for several parameters such as grain size, fruit skin and leaves. RAPD was performed using 10 RAPD primers. The results showed that the optimum 10% Chelex Chelex-TE isolation method was a sample size of 1 grain. The amplification of local Sumatran citrus and imported citrus using 10 single primers produced polymorphic bands. The value of jaccard's similarity indicates that the five samples of Sumatera local variety of citrus and imported citrus have high genetic variation. Indonesia has local varieties of citrus that are no less than imported citrus, especially in terms of fruit freshness. However, people are more interested in the color of citrus peel so people prefer imported citrus to local citrus, especially in Sumatera. Therefore, it is necessary to carry out efforts to conserve and improve the characteristics of these citrus to improve their quality through plant breeding. This study aims to optimize DNA isolation methods for citrus fruit samples with Chelex-TE and to determine the genetic profile of local Sumatera citrus and imported citrus using the genotyping RAPD. The samples used were several local citrus in Sumatera (Citrus Siam Mountain Omeh, Citrus Madu, Citrus Keprok Maga, Citrus Keprok Brastepu and Citrus Pasaman) and imported Citrus (Citrus Sunkist, Citrus Clemengold, Citrus Murkot and Citrus Wokam). DNA was isolated using the 10% Chelex-TE method which was optimized for several parameters such as grain size, fruit skin and leaves. RAPD was performed using 10 RAPD primers. The results showed that the optimum 10% Chelex Chelex-TE isolation method was a sample size of 1 grain. The amplification of local Sumatran citrus and imported citrus using 10 single primers produced polymorphic bands. The value of jaccard's similarity indicates that the five samples of Sumatera local variety of citrus and imported citrus have high genetic variation.
Co-Authors Abdul Rasak Abdul Razak Abdul Razak Achyar, Afifatul Afandi, Echa Azkia Afriandi, Muhammad Agusminarti Ahmad Fauzan Ahmad Saleh Akbar, Imam Qodri Ambiyar, Ambiyar Ananda, Azwir Anggun, Septiasri Anisha S Atifah, Yusni Atmazaki Atmazaki Ayesa, Putri Ayu, Duni Azalia, Salsa Novela Azeli, Saskia Putri Azwir Anhar Azzahra, Yolanda Belia Putri BR Tarigan, Siti Nadiah Zahra D. N. Putri, Dini Daniel Happy Putra Darussyamsu, Rahmawati Dezi Handayani Diana Susanti Dualom, Siti Dwi Hilda Putri Efdillah, Syilvia Eriza, Deni Septia Evi Suryawati Fachry Abda El Rahman Fadhil Ramadhan, Bintang FAJRINA, SUCI Ferdyan, Rhavy Festiyed Fidela, Wita Fitri Arsih Fitri, Anisa Fitri, Lel Fitria, Davina Fitria, Yanti Gunaria Siagian Haryanto Haryanto Hastutin, Sri Yuni Hayu, Elvira Heffi Alberida Indra Hartanto Iqra, Kuntum Nurul Isnaini Isnaini Jörg Gromoll K. Sari, Jelly Keiko Kasy Billah Keiko Kasy Billah Linda Wati Lufri Lufri Marcloryne Agnesia Masyitoh Masyitoh Maulina, Adinda Rizky Meisya, Divia Yuda Miftahul Jannah Monica, Della Trya Muhammad Ikbal Mutia Dwi Herakasih Mutia, Ravena Najib Rahman. G Neng Sholihat Nining Nuraida Nukman Moeloek Nurbaiti, Desy NURUL HIDAYAH P. Ningky, Yiyin Purnomo Soeharso Rahma Amelia, Anisha Chahya Rahmadhani Fitri Rahmawati Darussyamsu Ramadhan Sumarmin Ratna Nila Sari Regina Revi Gina Gunawan Rinti Mutiara Sari Samsudin, Syakirah Septelia I. Wanandi Siregar, Titik Ermila Sri Suciati Ningsih SUCI FAJRINA Syafrilianto, Syafrilianto Syari, Elsy Melia T. Berlian Wahyuni, Nur Anisa Winda Ayu Fietri Yerimades Yerimades Yerimadesi Yuda Meisya, Divia Yusi Febriani Zalni, Tita Yunia Zega, Dwi Junita Zhafira