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Journal : Bionature

Indole acetic acid (IAA) product Indole acetic acid (IAA) production by endophytic bacteria from Stem And Roots Of Indian Ash Tree (Lannea Coromandelica): Indian Ash Tree (Lannea Coromandelica) Hala, Yusminah; Ali, Alimuddin; Suryani, A. Irma
Bionature Vol. 25 No. 2 (2024): Oktober-Desember 2024
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v25i2.4618

Abstract

The endophytic bacteria live in healthy plants and can even improve plant health and productivity by suppressing pathogenic bacteria and increasing plant growth due to the producing IAA hormone. The purpose of this study was to describe the ability of each bacterial isolate to produce IAA hormone, to determine the fluctuations that occur during the five-day incubation period. The endophytic bacterial isolates from the roots of the Indian ash tree are A1 Bacillus, A2 Bacillus, A3 Bacillus, A4 Pseudomonas and A5 Bacillus, while from the stems are B1 Bacillus, B2 Agrobacterium, B3 Bacillus, B4 Bacillus and B5 Agrobacterium. The potential of bacterial isolates in producing IAA hormone was obtained by measuring the absorbance value using a spectrophotometer at a wavelength of 530 nm which was carried out every day for five days of incubation. The results obtained showed that endophytic bacterial isolates from the roots and stems of Kayu Jawa Plants have the ability to produce IAA with the highest concentration on 3 days of incubation was produced by isolate A3 (7.95 ppm), from the genus Bacillus, on 4 days of incubation the highest concentration was produced by A2 (22.18 ppm) from the genus Bacillus and on 5 days of incubation the highest concentration was produced by A2 (57.60 ppm) from the genus Bacillus.
Selection and Characterization of Antagonistic Bacteria Isolated from the Rhizosphere of Potato Plants (Solanum tuberosum L.) Against the Fungus Causing Fusarium Wilt Disease In Vitro Hilda Karim; Putri Ramadhani; Nani Kurnia; Ali, Alimuddin
Bionature Vol. 27 No. 1 (2026): (In Press) April - Juni 2026
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/jkxp9591

Abstract

Fusarium wilt, caused by Fusarium oxysporum (Fo), is a major disease affecting potato plants (Solanum tuberosum L.) and contributes significantly to yield losses. This study aimed to isolate and identify antagonistic bacteria from the potato rhizosphere capable of inhibiting the growth of F. oxysporum and to assess their antifungal potential through in vitro analysis. Soil samples were collected from the potato-growing region of Kanreapia, Gowa Regency, Indonesia. The antagonistic potential of the bacterial isolates was evaluated using the dual-culture assay. A total of eight bacterial isolates demonstrated significant inhibitory effects against Fo, representing six genera: Clostridium (75.0%), Bacillus (69.3% and 68.9%), Pseudomonas (67.9% and 62.1%), Staphylococcus (64.8%), Neisseria (64.7%), and Serratia (61.5%). Among these, the isolate identified as Clostridium exhibited the highest inhibition rate (75.0%), while Serratia showed the lowest (61.5%). These findings suggest the potential application of rhizobacteria as biological control agents for managing Fusarium wilt in potatoes.