Garuda - Garba Rujukan Digital

Formulasi Emulgel yang Mengandung Ekstrak Etanol Daun Binahong (Anredera cordifolia (Ten.) Steenis) dan Uji Aktivitasnya terhadap Propionibacterium acnes secara In Vitro Yani, Tri Nofi; Anwar, Effionora; Saputri, Fadlina Chany
Jurnal Kefarmasian Indonesia VOLUME 6, NOMOR 2, AGUSTUS 2016
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v6i2.2923

Binahong (Anredera cordifolia (Ten.) Steenis) leaves have been known to have antibacterial activity but the activity against Propionibacterium acnes as one of the bacteria that play a role in acne’s pathogenesis is still unknown. Binahong leaves ethanolic extract containing 1,28% of ursolic acid in this study were tested by in vitro against Propionibacterium acnes giving result minimum bactericidal concentration of 0,05%. Emulgel of binahong leaves ethanolic extract in this study had physical stability for 12 weeks. Inhibition zone of binahong leaves ethanolic extract emulgel is larger than clindamycin phosphate 1,2% against Propionibacterium acnes, which is in formula 1 (extract equivalen with MIC 0,05%) is 19,67±1,25 mm and formula 2 (extract equivalen double MIC 0,05%) is 20,67±0,47 mm, while inhibition zone of clindamycin phosphate 1,2% is 16,3±0,47 mm.

Potensi Ekstrak Biji Coklat (Theobroma cacao Linn) sebagai Inhibitor Tirosinase untuk Produk Pencerah Kulit Kurniasari, Aprilia; Anwar, Effionora; Djajadisastra, Joshita
Jurnal Kefarmasian Indonesia VOLUME 8, NOMOR 1, FEBRUARI 2018
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v8i1.3723

Hyperpigmentation is a condition of excessive skin pigments production. The skin colour is strongly influenced by the presence of melanin that marked by the melanin tyrosinase enzyme activity. Cocoa (Theobroma cacao Linn) is one of the ingredients which are rich in flavonoids include polyphenolic compounds that used as antioxidants and a tyrosinase inhibitor. The aim of this study is to examine the potential of the cocoa bean extract as a tyrosinase inhibitor for skin lightening active ingredients. The method of the study was experimental laboratories, among others: total flavonoid and tyrosinase inhibitory activity assay. The result of this research was ethanol extract of cocoa had tyrosinase inhibitor activity. The inhibitory activity could be seen from the IC50 for monophenolase activity were 352.05 μg mL-1 and for diphenolase activity 836.20 μg mL-1 respectively. This value is greater than kojic acid, for monophenolation was 2.38 μg mL-1 and diphenolation was 10.74 μg mL-1. The total flavonoids content was 0.05% w/w so that the ethanol extract of the cocoa bean is a natural product that potential to be used in the formulation of skin lightening cream in the pharmaceutical sciences.

In Silico Study of Inhibition of Activation Of 1A52 Ethanol Extract Leunca Fruit (Solanum nigrum L) Fajar, In Rahmi Fatria; Anwar, Effionora; Mumpuni, Esti; Desmiaty, Yesi; Agustini, Kurnia
SANITAS: Jurnal Teknologi dan Seni Kesehatan Vol 15 No 2 (2024): SANITAS Volume 15 Nomor 2 Tahun 2024
Publisher : Politeknik Kesehatan Kemenkes Jakarta II

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36525/sanitas.2024.516

The development of science has found a way to prevent skin aging in menopausal women. One of the therapies is estrogen replacement therapy (17β estradiol). Skin aging in menopausal women is associated with decreased ERα expression. Leunca fruit (Solanum nigrum L) is known to contain phytoestrogens and has the potential as an antiaging agent. The purpose of this study was to predict the anti-aging effect of Solanum nigrum ethanol extract compounds through the Erα 1A52 [A] activation pathway. The research method of active substance analysis metabolite profiling was carried out by analyzing physicochemical properties using pkCSM. This analysis was carried out as an initial selection of compound safety for therapy. Molecular docking with the MVD (Free trial) application. The RMSD value ≤ 2Å indicates a valid docking and the application can be used. The results of the in silico study showed that the results of metabolite profiling showed that diosgenin was an agonist against ERα. The compound has physicochemical properties according to the Lipinski standard so that it can bind to ERα. The conclusion is that diosgenin has the potential and can act as a 1A52 [A] inhibitor so it can be used as an antiaging agent.

Novel Enhalus acoroides Phytosomes: Formulation, Characterization, and Bioavailability Enhancement Siti Mardiyanti; Effionora Anwar; Yesi Desmiaty; Siti Sadiah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 7 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i7.1333

Background: Enhalus acoroides (seagrass) possesses valuable bioactive compounds, including quercetin, with potential therapeutic applications, notably antidiabetic effects. However, the poor solubility and low bioavailability of compounds like quercetin limit their clinical efficacy. Phytosomes, phospholipid-based nanocarriers, represent a promising strategy to overcome these limitations. This study aimed to develop and characterize E. acoroides extract-loaded phytosomes to enhance its potential bioavailability. Methods: E. acoroides was collected, processed, and extracted using ultrasound-assisted extraction (UAE). Total phenolic (TPC) and flavonoid content (TFC) were determined. Phytosomes were prepared using the thin-layer hydration method with varying extract-to-soya lecithin ratios (F1=1:1, F2=1:2, F3=1:3). Characterization involved particle size analysis, zeta potential measurement, Fourier Transform Infrared Spectroscopy (FTIR), Transmission Electron Microscopy (TEM), entrapment efficiency (EE%) determination via HPLC, and in vitro dissolution studies. Results: The UAE extract yielded TPC of 0.318 ± 0.036 mg GAE/g and TFC of 1.023 ± 0.022 mg QE/g. Phytosome formulation F1 (1:1 ratio) exhibited optimal characteristics: particle size of 276.4 nm, PDI of 0.591, zeta potential of -18.0 mV, EE% of 80.47 ± 2.62%, and a spherical morphology. FTIR confirmed complexation. F1 phytosomes demonstrated significantly enhanced dissolution, releasing 87.13% of the entrapped compound over 12 hours compared to the crude extract. Conclusion: E. acoroides extract was successfully encapsulated into phytosomes using a thin-layer method. The F1 formulation (1:1 extract:phospholipid ratio) demonstrated favorable physicochemical properties (nanoparticle size, moderate stability, high EE%) and markedly improved in vitro dissolution, suggesting enhanced bioavailability potential for E. acoroides phytoconstituents.

Novel Enhalus acoroides Phytosomes: Formulation, Characterization, and Bioavailability Enhancement Siti Mardiyanti; Effionora Anwar; Yesi Desmiaty; Siti Sadiah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 7 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i7.1333

Background: Enhalus acoroides (seagrass) possesses valuable bioactive compounds, including quercetin, with potential therapeutic applications, notably antidiabetic effects. However, the poor solubility and low bioavailability of compounds like quercetin limit their clinical efficacy. Phytosomes, phospholipid-based nanocarriers, represent a promising strategy to overcome these limitations. This study aimed to develop and characterize E. acoroides extract-loaded phytosomes to enhance its potential bioavailability. Methods: E. acoroides was collected, processed, and extracted using ultrasound-assisted extraction (UAE). Total phenolic (TPC) and flavonoid content (TFC) were determined. Phytosomes were prepared using the thin-layer hydration method with varying extract-to-soya lecithin ratios (F1=1:1, F2=1:2, F3=1:3). Characterization involved particle size analysis, zeta potential measurement, Fourier Transform Infrared Spectroscopy (FTIR), Transmission Electron Microscopy (TEM), entrapment efficiency (EE%) determination via HPLC, and in vitro dissolution studies. Results: The UAE extract yielded TPC of 0.318 ± 0.036 mg GAE/g and TFC of 1.023 ± 0.022 mg QE/g. Phytosome formulation F1 (1:1 ratio) exhibited optimal characteristics: particle size of 276.4 nm, PDI of 0.591, zeta potential of -18.0 mV, EE% of 80.47 ± 2.62%, and a spherical morphology. FTIR confirmed complexation. F1 phytosomes demonstrated significantly enhanced dissolution, releasing 87.13% of the entrapped compound over 12 hours compared to the crude extract. Conclusion: E. acoroides extract was successfully encapsulated into phytosomes using a thin-layer method. The F1 formulation (1:1 extract:phospholipid ratio) demonstrated favorable physicochemical properties (nanoparticle size, moderate stability, high EE%) and markedly improved in vitro dissolution, suggesting enhanced bioavailability potential for E. acoroides phytoconstituents.

Optimizing Ultrasonic Extraction of Enhalus acoroides to Enhance Physicochemical Traits and Antioxidant Activity Mardiyanti, Siti; Anwar, Effionora; Desmiaty, Yesi; Sadiah, Siti
Jurnal Jamu Indonesia Vol. 10 No. 3 (2025): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v10i3.419

Enhalus acoroides is a seagrass species known for its antioxidant potential due to its flavonoid content, including quercetin. This study aimed to optimize the extraction time using the ultrasound-assisted extraction (UAE) method based on physicochemical parameters and antioxidant activity. The simplicia was extracted for 20, 30, and 40 minutes, each in triplicate, using 96% ethanol acidified with 1% HCl. The results demonstrated that extraction time significantly influenced the physicochemical properties, including yield (highest at 30 minutes: 40.2%), total phenolic content (1.153 mg GAE/g), total flavonoid content (0.318 mg QE/g), quercetin content (0.316 mg/g), and antioxidant activity (IC₅₀ = 58.44 ppm), whereas no significant effect was observed on total ash content. Qualitative phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, saponins, and triterpenoids. Based on these findings, a 30-minute extraction time is recommended as the optimal condition for obtaining E. acoroides extract with the highest antioxidant activity.

Antioxidant Activity Cultivated of Soursop (Annona muricata L.) Leaves Extract in Jayasari Village, Pangandaran District, West Java Zahara, Indah; Anwar, Effionora; Laksmitawati, Dian Ratih; Rahmat, Deni
Journal of Natural Product for Degenerative Diseases Vol. 1 No. 1 (2023): JNPDD September
Publisher : Faculty of Pharmacy Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58511/jnpdd.v1i1.5317

Indonesia is a source of raw materials for tropical medicines that treat various diseases. One of the places to plant soursop leaves in West Java is Jayasari Village, Pangandaran District. Annona muricata Linn (Soursop) leaves is a medicinal plant widely used as an anti-diabetic, anti-inflammatory, insecticide, antimalarial, anticancer, antibacterial, and antioxidant. Soursop leaves have many benefits because they contain phytochemical compounds. This study aims to determine the phytochemical content, total flavonoid content, and antioxidant activity of A. muricata leaves. This research was carried out in several steps, including extraction, evaporation, phytochemical testing, total flavonoid content, and antioxidant activity. Phytochemicals were extracted with 96% ethanol by maceration. Measurement of flavonoids in extracts was determined using standard quercetin and UV-Vis spectrophotometry method. Antioxidant activity was carried out through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical removal test. The results showed that the ethanol extract of soursop leaves contained alkaloids, flavonoids, phenolics, saponins, and tannins. The results of measurements of flavonoids from the ethanol extract of soursop leaves showed that 1 g of the extract contained 8.32 mg of quercetin equivalent. The ethanol extract of soursop leaves has antioxidant activity, as indicated by the scavenging of DPPH radicals with an IC50 of 56.73 ppm.

Anti-inflammatory Activity of Glucomannan Resulting from β-mannanase Enzyme Hydrolysis in RAW 264.7 Cells Nuraini, Nuraini; Effionora Anwar; Dian Ratih Laksmitawati; Hendig Winarno; Siti Irma Rahmawati
Journal of Natural Product for Degenerative Diseases Vol. 2 No. 2 (2025): JNPDD March
Publisher : Faculty of Pharmacy Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58511/jnpdd.v2i2.7797

Glucomannan is a polysaccharide of the hemicellulose type, composed of glucose and mannose chains. It has the ability to form solutions that expand and thicken. The bioactivity of carbohydrates is influenced by the length of their saccharide chains. Hydrolysis can alter the structural dimensions of polysaccharides, including molecular weight, type, number, and position of substituent groups, which in turn impacts their biological activity. Various methods can modify carbohydrate structures to enhance their value, with degradation being one such method. Enzymatic degradation is particularly advantageous due to its high specificity, efficiency, and minimal side effects. Numerous studies have identified glucomannan as having immunomodulatory effects on macrophage cells. When stimulated with lipopolysaccharides, macrophages release various cytokines and inflammatory mediators, such as TNF-α, iNOS, and NO. This study explored the potential anti-inflammatory effects of glucomannan (GM) and its enzymatically hydrolyzed form (Enz-GM) on RAW 264.7 cells. The research involved degrading glucomannan using the β-mannanase enzyme, followed by in vitro testing of the degradation product (Enz-GM) for anti-inflammatory activity. This was achieved using an ELISA kit to measure the expression of IL-6, TNF-α, and iNOS. The results showed that Enz-GM at pH 9 (Mw 1.24 kDa) significantly reduced the pro-inflammatory cytokines IL-6 and iNOS (p ≤ 0.05) compared to GM, while TNF-α expression did not show a significant difference between Enz-GM and GM.

FORMULASI GEL MENGGUNAKAN SERBUK DAGING IKAN HARUAN (Channa striatus) SEBAGAI PENYEMBUH LUKA Rahmawanty, Dina; Anwar, Effionora; Bahtiar, Anton
Media Farmasi: Jurnal Ilmu Farmasi Vol. 11 No. 1: Maret 2014
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/mf.v11i1.1395

Daging ikan haruan (Channa striatus) dipercaya dapat digunakan untuk menyembuhkan luka karena mengandung protein, asam amino esensial, lemak dan asam lemak yang berperan dalam prosesÂ penyembuhan luka. Tujuan dari penelitian ini ialah membuat gel yang mengandung serbuk daging ikan haruan sebagai penyembuh luka. Pada penelitian ini digunakan serbuk daging ikan haruan (Channa striatus) sebagai zat aktif sebanyak 1 gram pada formula 1 dan 2 gram pada formula 2. Serbuk daging ikan haruan dibuat suspensi dengan ukuran partikel nanometer dengan metode gelasi ionik menggunakan kitosan dan natrium tripolifosfat, kemudian dibuat menjadi bentuk sedian gel dengan menggunakan gelling agent HPMC. Suspensi yang dihasilkan dilakukan karakterisasi fisika dan kimia. Hasil karakterisasi suspensi formula 1 dan formula 2 adalah sebagai berikut : ukuran partikel berturut-turut 491,8 - 665,5 nm, 481,8 - 828,1 nm; indeks polidispersitas 0,512, 0,456; nilai potensial zeta (+)29,15mV, (+)29,35mV; kedua formula mempunyai partikel berbentuk sferis. Sediaan gel yang dihasilkan dievaluasi aktivitas penyembuhan luka secara in vivo. Dari hasil uji in vivo sediaan gel serbuk daging ikan haruan dapat digunakan sebagai penyembuh luka. Kata Kunci: gel, gelasi ionik, haruan (Channa striatus), kitosan, natrium tripolifosfat, luka.

Perbandingan Ekstrak Jahe Merah (Zingiber Officinale Roscoe. Var. Rubrum), Gingerol dan Shogaol sebagai Anti-Toksoplasma terhadap Parasit Toxoplasma Gondii Secara In-Vitro Sa’diah , Siti; Anwar, Effionora; Jufri, Mahdi; Cahyaningsih, Umi
Jurnal Jamu Indonesia Vol. 4 No. 3 (2019): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v4i3.160

Metode ekstraksi dan jenis pelarut yang digunakan akan berdampak pada proses penarikan komponen aktif sehingga akan berpengaruh juga pada aktivitasnya. Jahe merah (Zingiber officinale var. Rubrum) merupakan salah satu bahan rempah yang juga berpotensi sebagai tanaman obat. Salah satu khasiat ekstrak jahe merah adalah sebagai anti toksoplasma. Pada penelitian ini telah dilakukan pembuatan ekstrak jahe merah dengan dua jenis metode yaitu maserasi dan sokhletasi masing-masing menggunakan tiga jenis pelarut (etanol 30%, etanol 70% dan etanol 96%) sehingga diperoleh enam jenis ekstrak. Kemudian masing-masing ekstrak ditentukan kadar senyawa pencirinya meliputi 6-gingerol, 8-gingerol, 10-gingerol dan 6-shogaol dengan Kromatografi Cair Kinerja Tinggi (KCKT). Selanjutnya ekstrak diuji aktivitas antitoksoplasma secara in vitro terhadap parasit Toksoplasma gondii pada fase takhizoit yang ditumbuhkan pada sel vero. Penentuan aktivitas antitoksoplasma juga dilakukan pada senyawa murni 6, 8, 10-gingerol dan 6-shogaol serta kontrol positif antibiotik spiramisin. Hasilnya menunjukkan bahwa ekstrak etanol 96% dengan metode maserasi adalah yang paling baik aktivitasnya dan 6-gingerol adalah senyawa penciri yang paling berperan sebagai anti-toksoplasma.

Title

Found 40 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 40 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 40 Documents
Search