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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Perikanan Universitas Gadjah Mada Jurnal Intek Akuakultur Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Jurnal Penelitian Perikanan Indonesia Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Journal of Tropical Marine Science Jurnal Abdi Insani Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan

Harton Arfah

Departemen Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Dramaga Bogor 16680

Author-ID : 231540

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Social Sciences Veterinary

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PENGARUH PEMBERIAN HORMON ITe METILTESTOSTERON SECARA ORAL PADA INDI.,K IKAN GAPI (Poecilia retidlau Pcters) STRAIN TUXEDO TERHADAP JENIS KELAMIN KETURUNANNYA Nur Rachmawaty Arma; Lilis Mulyati; Komar Sumantadinata; Muhammad Zairin Jr; Harton Arfah
Jurnal Penelitian Perikanan Indonesia Vol 1, No 3 (1995): (Vol.1 No.3 1995)
Publisher : Pusat Riset Perikanan, BRSDM KP.

Percobaan dilakukan untuk mengetahui pengaruh pemberian hormon lzo-metiltestosteron secara oral pada induk ikan gapi (Poecilia reticulau Peters) strain tuxedo terhadap ienis kelamin keturunannya.

Performa Pertumbuhan Hibrida Antara Ikan Kerapu Batik Betina (Epinephelus microdon) Dengan Ikan Kerapu Kertang Jantan (E. lanceolatus) yang Dipelihara Pada Salinitas Berbeda Ahmad Fahrul Syarif; Dinar Tri Seolistyowati; Harton Arfah
Journal of Tropical Marine Science Vol 2 No 1 (2019): Journal of Tropical Marine Science
Publisher : Universitas Bangka Belitung

Hibridisasi antara spesies ikan kerapu batik (Epinephelus microdon) betina dan ikan kerapu kertang (E. lanceolatus) jantan terus dikembangkan untuk memperoleh strain baru dengan performa unggul. Penelitian ini bertujuan untuk mengevaluasi performa pertumbuhan hibrida ikan kerapu “TIKTANG” (hibridisasi antara ikan kerapu batik betina x ikan kerapu kertang jantan) terhadap perbedaan salinitas 10, 20 dan 30 ppt pada beberapa parameter pertumbuhan panjang dan bobot. Percobaan dirancang menggunakan Rancangan Acak Lengkap (RAL) dengan tiga perlakuan perbedaan salinitas dan masing-masing diulang tiga kali. Pemeliharaan dilakukan selama 21 hari dengan metode pemberian pakan at satiation. Hasil penelitian menunjukkan bahwa derajat kelangsungan hidup ikan kerapu hibrid “TIKTANG” selama pemeliharaan sebesar 100%, sedangkan performa pertumbuhan baik panjang maupun bobot menunjukan perlakuan 10 ppt lebih rendah dibandingkan 20 dan 30 ppt (p<0,05). Hibridisasi antara spesies ikan kerapu batik (Epinephelus microdon) betina dan ikan kerapu kertang (E. lanceolatus) jantan terus dikembangkan untuk memperoleh strain baru dengan performa unggul. Penelitian ini bertujuan untuk mengevaluasi performa pertumbuhan hibrida ikan kerapu “TIKTANG” (hibridisasi antara ikan kerapu batik betina x ikan kerapu kertang jantan) terhadap perbedaan salinitas 10, 20 dan 30 ppt pada beberapa parameter pertumbuhan panjang dan bobot. Percobaan dirancang menggunakan Rancangan Acak Lengkap (RAL) dengan tiga perlakuan perbedaan salinitas dan masing-masing diulang sebanyak tiga kali. Pemeliharaan dilakukan selama 21 hari dengan metode pemberian pakan secara at satiation. Hasil penelitian menunjukkan bahwa derajat kelangsungan hidup ikan kerapu hibrid “TIKTANG” selama pemeliharaan sebesar 100%, sedangkan performa pertumbuhan baik panjang maupun bobot menunjukan perlakuan 10 ppt lebih rendah dibandingkan 20 dan 30 ppt (p<0,05)

EVALUATION OF RESISTANCE AND GENE EXPRESSION OF BARRAMUNDI, Lates calcarifer POST-INFECTION OF NERVOUS NECROSIS VIRUS Fitriyah Husnul Khotimah; Alimuddin Alimuddin; Dinar Tri Soelistyowati; Sri Nuryati; Ketut Sugama; Ahmad Muzaki; Indah Mastuti; Sari Budi Moria Sembiring; Ketut Mahardika; Harton Arfah; Haryanti Haryanti
Indonesian Aquaculture Journal Vol 17, No 2 (2022): (December, 2022)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.17.2.2022.97-106

The most common problem in barramundi Lates calcarifer seedling production is the high mortality (> 90%) caused by nervous necrosis virus (NNV) infection. This research aims to evaluate the resistance and gene expression of barramundi challenged by NNV. Two populations were used in this study, i.e., Australian, and Situbondo-originated barramundi populations. The immune-related gene expression levels in the liver, head of kidney, and spleen were observed at 48 and 96 hours after post-infection (hpi). Barramundi’s survival and blood parameters were evaluated post-NNV infection. The results showed that the highest survival was revealed in Situbondo’s barramundi (42.0±4.47%) compared to Australian barramundi (20.0±7.07%) and no mortality was observed in the control without NNV infection. The higher survival rate in barramundi from Situbondo was in line with the blood profile. The number of red blood cell from Situbondo barramundi post-NNV infection (ST) at 96 hpi was higher (P<0.05) than Australian barramundi post-NNV infection (AT). The number of white blood cell of ST at 48 hpi was higher (P<0.05) than AT, but started to decrease at 96 hpi in ST barramundi. The total white blood cell in AT barramundi increased from 48 to 192 hpi. TNFα and IL1-β gene expression levels were significantly higher in the liver, head kidney, and spleen of Situbondo compared to Australian barramundi at 48 hpi, while MHCIIα gene expression in Situbondo’s was significantly higher compared to Australian barramundi at 96 hpi. These results indicate the important roles of all the genes in the barramundi’s immune responses against viral infection. Based on the results of the research, Situbondo’s barramundi has the potential to be used as a candidate for generating broodstock of disease-resistant strain.

Effect of oodev and turmeric powder dietary supplementations on the reproductive performance of sunset platy fish Xiphophorus maculatus Harton Arfah; Odang Carman; Muhamad Fathurrizki Darmawan; Fajar Maulana; Lina Mulyani
Jurnal Akuakultur Indonesia Vol. 22 No. 1 (2023): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.22.1.27-35

Plati fish is a freshwater ornamental fish with great demand due to its beautiful body shape and color, besides the price is affordable.The main problem faced by plati fish farmers is that the production targets have not been achieved to meet the market needs. This study aimed to examine the effect of the oodev and turmeric powder and the combination of both added into the fish diet on the reproductive performance of plati fish broodstock. The experimental design in this study used a completely randomized design with four treatments, i.e treatment A (control), B (oodev 0.5 ml/kg broodstock), C (turmeric powder 3%/kg feed), and D (oodev 0.5 ml/kg broodstock + turmeric powder 3%/kg feed). There were three replications for each treatment. The observed parameters included the percentage of pregnant broodstocks, the percentage of labored broodstocks, the frequency of labored broodstocks, larvae production, and the survival rate of the larvae. The data were analyzed using ANOVA and further tested with Duncan's test with a 95% confidence level. The results showed that the addition of oodev, turmeric flour, and a combination of both into the feed every one week increased the average production of larvae. Total larval production in all treatments was sorted from the highest in treatment D, B, C, and and the lowest was A treatment with larvae production of 428, 251, 209, and 93, respectively. The addition of oodev 0.5 ml/kg broodstock, turmeric powder 3%/kg feed, and the combination of both into the feed increased larval production three, two, and five times higher compared to the control, respectively. The percentage of survival of the larvae is sorted from the highest in treatment D, B, C, A, i.e. 97.51%, 95.79%, 95.65%, and 93.32%, respectively. Keywords: larvae, oodev, platy, turmeric powder ABSTRAK Ikan plati merupakan ikan hias air tawar yang banyak diminati masyarakat karena memiliki bentuk dan warna tubuh yang indah, selain itu harganya yang terjangkau membuat ikan ini laris di pasaran. Permasalahan utama yang dihadapi pembudidaya ikan plati adalah belum tercapainya target produksi untuk memenuhi kebutuhan pasar. Penelitian ini bertujuan mengkaji pengaruh penambahan oodev dan tepung kunyit serta kombinasi keduanya yang diberi pada pakan terhadap performa reproduksi induk ikan plati. Rancangan percobaan pada penelitian ini menggunakan rancangan acak lengkap dengan empat perlakuan, yaitu: perlakuan A (kontrol), B (oodev 0,5 ml/kg induk), C (tepung kunyit 3%/kg pakan), dan D (oodev 0,5 ml/kg induk + tepung kunyit 3%/kg pakan). Terdapat tiga ulangan pada masing-masing perlakuan. Parameter penelitian yang diamati yakni persentase induk bunting, persentase induk melahirkan, frekuensi induk melahirkan, produksi larva, dan tingkat kelangsungan hidup larva. Data diolah menggunakan ANOVA dan diuji lanjut dengan uji Duncan dengan selang kepercayaan 95%. Hasil menunjukkan bahwa penambahan oodev, tepung kunyit, dan kombinasi keduanya pada pakan setiap satu minggu dapat meningkatkan rata-rata produksi larva yang dihasilkan. Produksi larva total diurut dari yang terbanyak adalah perlakuan D (428 ekor), B (251 ekor), C (209 ekor), dan terendah adalah A (93 ekor). Penambahan oodev 0,5 ml/kg induk, tepung kunyit 3%/kg pakan, dan kombinasi keduanya pada pakan meningkatkan produksi larva sebanyak tiga, dua, dan lima kali dibanding kontrol. Persentase kelangsungan hidup larva diurut dari yang tertinggi terdapat pada perlakuan D (97,51%), B (95,79%), C (95,65%), dan terendah adalah A (93,32%). Kata kunci: larva, oodev, plati, tepung kunyit

Molecular Assessment of Kappaphycus alvarezii Cultivated in Tarakan based on cox2-3 Spacer Gloria Ika Satriani; Dinar Tri Soelistyowati; Alimuddin Alimuddin; Harton Arfah; Irzal Effendi
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 18, No 1 (2023): May 2023
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.736

The seaweed Kappaphycus alvarezii is a leading aquaculture commodity possessing high economic value that has been used as a raw material for various natural products, food, and pharmaceutical industries. The main challenge regarding its production, especially in Tarakan, is the supply of superior seeds. Therefore, this study aims to obtain phylogenetic information and the cultivation performance of the selected seeds by identifying genetic sources based on molecular markers. DNA sequencing was analyzed using the molecular marker cyclooxygenase 2-3 intergenic spacer (cox2-3 spacer) on the 16 cultivars collection from around Indonesia. Four haplotypes representing the K. alvarezii variant to be cultivated in Tarakan City were produced based on the phylogenetic clustering and further molecular analysis using a cox2-3 spacer mitochondrial DNA marker. These include the Kupang and Natuna varieties, also referred to as Lampung seedlings, which is a tissue culture plantlets used for development at SEAMEO-BIOTROP Bogor and propagated by the Lampung Marine Aquaculture Center (BBPBL), Tarakan. These varieties have genetic distances (pairwise comparisons based on the mean Kimura 2-parameter model) ranging from 0.000-0.243 units. The cultivation was carried out for 30 days in the waters of Tanjung Batu (Tj. Batu) Mamburungan, Tarakan, North Kalimantan. The result showed that the Lampung cultivar (Natuna) has a superior daily growth rate (DGR) and carrageenan yield compared to the local cultivars (Mamburungan Tanjung Batu (Takalar), Tarakan, and Kupang). Furthermore, the genetic analysis with cox2-3 spacer markers could be an effective seed selection tool for tracing genetic information in developing superior seedlings in aquaculture activities.

EVALUASI MASKULINISASI PLATI PEDANG, Xiphophorus helleri MENGGUNAKAN KOMBINASI EKSTRAK BUAH PINUS, Pinus merkusii DENGAN AROMATASE INHIBITOR Akbar, Muhamad Saepul; Sudrajat, Agus Oman; Arfah, Harton; Soelistyowati, Dinar Tri
Media Akuakultur Vol 19, No 1 (2024): Juni 2024
Publisher : Politeknik Kelautan dan Perikanan Bone

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.19.1.2024.9-15

Hormon 17α-methyltestostrone sudah banyak digunakan untuk maskulinisasi pada kegiatan budidaya namun, bahan tersebut bersifat karsinogenik sehingga diperlukan alternatif pengganti bahan tersebut. Penelitian ini bertujuan mengevaluasi efek kombinasi ekstrak buah pinus (Pinus merkusii) dan aromatase inhibitor pada ikan plati pedang. Sebanyak 5 perlakuan menggunakan bahan berbeda, yaitu 17α-methyltestostrone, ekstrak buah pinus, aromatase inhibitor, kombinasi ekstrak buah pinus dan aromatase inhibitor, serta kontrol dengan masing-masing 3 ulangan. Perendaman dilakukan selama 6 jam, kemudian ikan dipelihara selama 60 hari. Rasio induk jantan dan betina yang digunakan untuk pemijahan ikan pada penelitian ini adalah 1:3. Penelitian ini berfokus pada tiga parameter, yakni Tingkat Kelangsungan Hidup (TKH), Nisbah Kelamin Jantan (NKJ) dan jumlah ikan jantan yang dihasilkan pada tiap perlakuan. Hasil penelitian menunjukkan ikan yang direndam pada bahan berbeda menghasilkan nilai nisbah kelamin jantan yang lebih tinggi. Dosis terbaik pada penelitian ini adalah perendaman dengan ekstrak buah pinus dengan dosis 0,02 mL L-1.The hormone 17α-methyltestostrone has been widely used for masculinization in aquaculture, however, this material is carcinogenic and it is required to find its alternative substitutions. The aim of this research to evaluate the effects of combination of pine cone (Pinus merkusii) extract and aromatase inhibitor on swordtail fish. A total of 5 treatments using different materials, namely: 17-α methyltestosterone, pine cone extract, aromatase inhibitor, pine cone extract mixed with aromatase inhibitor, and control with 3 replications. Immersion was conducted for 6 hour and the the fish were kept for 60 days. The ratio of male to female parents used for fish spawning was 1:3. The study focused on three parameters, i.e. survival rate, male fish percentage, and number male fish produced. The results revealed fish immersed with different materials exhibited higher male fish percentage compared to the control group (not immersed). The best treatment in this research was immersion using pine cone extract with dosage of 0.02 mL L-1.

Sex Reversal on Congo Tetra Fish (Micraleptus intterruptus ) Larvae Arfah, Harton; Alimuddin, ,; Sumantadinata, K.; Ekasari, Julie
Jurnal Akuakultur Indonesia Vol. 1 No. 2 (2002): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTExperiment was performed to assess the effect of 17a-methyltestosterone (MT) treatment on Congo tetra fish larvae. To evaluate the optimal pattern of MT treatment, three different treatments were administrated. Three months old larvae were submerged in three different doses of MT; 1, 2 and 4 mg/l. These studies showed that the highest percentage of male fish was obtained by 4 mg/l MT treatment, 87,17%. The 2 mg/l and 1 mg/l MT treatments obtained 77,53% and 69,86% male respectively, two times higher than control, 38,96%. On the other hand, the 4 mg/l MT treatment also resulted the highest percentage of hermaphrodite fishes, 17,58%. The highest survival rate was shown by 1 mg/l MT treatment, 62,77% and the lowest was shown by the 4 mg/l MT treatment, 47,20%. The highest rate of fish length and weight was shown by the 4 mg/l MT treatment, 4,4 cm and 1,65 gram respectively. These findings suggest that MT treatment offers an advantage in growth of tetra Congo larvae. Key word : Sex reversal, methyltestosterone, Congo tetra fish, Micraleptus intterruptus. ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh perendaman larva di dalam larutan hormon 17a-metiltestosteron pada dosis 1, 2 dan 4 mg/l larutan. Persentase tertinggi ikan jantan dihasilkan oleh perlakuan 4 mg/l, yaitu 87,17%. Perlakuan 2 mg/l dan 1 mg/l masing-masing menghasilkan 77,53% dan 69,86% sedangkan kontrol menghasilkan 38,96% jantan. Efek lain dari perlakuan MT ini adalah hermafroditisme. Perlakuan 4 mg/l menghasilkan persentase hermafrodit tertinggi yaitu 17,58%, sedangkan pada kontrol kelangsungan hidup tertinggi diperoleh pada perlakuan 1 mg/l (62,77%) dan terendah pada perlakuan 4 mg/l (47,20%). Hasil tersebut menunjukkan adanya pengaruh dosis hormon terhadap kelangsungan hidup ikan. Pengukuran bobot dan panjang ikan pada setiap perlakuan menunjukkan nilai tertinggi dihasilkan oleh perlakuan 4 mg/l yaitu 1,65 gram dan 4,40 cm. Hal ini diduga bahwa hormon MT merangsang pula pertumbuhan ikan.Kata kunci : Pergantian kelamin, metiltestosteron, ikan tetra Kongo, Micraleptus intterruptus.

Utilization of MS 222 in Transport of Catfish (Pangasius sutchi) Seed Arfah, Harton; Supriyono, Eddy
Jurnal Akuakultur Indonesia Vol. 1 No. 3 (2002): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTProblems faced in supplying seed out of Java especially Bogor to Sumatera, for instance, are it only contains few seed (100 seeds/l) and it has a high mortality rate because of stress during transport. This experiment was conducted to know the effect of MS 222 in keeping fish metabolism activity down while taking note of its survival rate and change of water quality. The experimental design used here was factorial design 3x5 with 3 repetitions. Dosages of MS–222 were 0, 25 and 50 ppm and the fish densities were 100, 300, 400, 500 and 600 per liter. As container was plastic bag (volume 10 l). After putting the fish into the plastic bag, it was filled up with oxygen, which the volume was 3 times than water volume, than the plastic bag was closed by tying it. After 18 hours of treatment the survival rate and water quality were checked. Based on evaluation of survival rate, it was concluded that the combination between 25 ppm of MS 222 and fish density, which was 500 per liter, gave an optimum result.Keywords: Catfish, Pangasius sutchi, live transport, stress, MS–222, survival rate ABSTRAKKendala yang dihadapi dalam pemasokan benih ikan patin dari pulau Jawa terutama Bogor, keluar pulau Jawa terutama Sumatera adalah jumlah ikan yang terangkut masih sedikit (± 100 ekor/l) dan tingkat kematian yang cukup tinggi, karena stres selama perjalanan. Penelitian ini dilakukan untuk mengetahui pengaruh penggunaan MS–222 dalam menekan aktivitas metabolisme ikan dengan memperhatikan tingkat kelangsungan ikan dan perubahan kualitas air. Penelitian ini menggunakan rancangan faktorial 3x5 dengan 3 ulangan. Dosis MS–222 yang digunakan: 0,25 dan 50 ppm dan kepadatan ikan: 100, 300, 400, 500 dan 600 ekor/l. Wadah pengangkutan berupa kantong plastik (volume 10 l). Ikan yang diangkut dimasukkan ke dalam kantong plastik, kemudian dipompakan oksigen sebanyak 3 kali volume air, selanjutnya dilakukan pengikatan. Setelah 18 jam perlakuan dilakukan perhitungan terhadap tingkat kelangsungan hidup ikan dan perubahan kualitas air media. Hasil penelitian ini menunjukkan kombinasi penggunaan MS–222 25 ppm dan kepadatan ikan 500 ekor/l memberikan tingkat kelangsungan hidup yang optimal.Kata kunci: Ikan patin, Pangasius sutchi, pengangkutan hidup, stres, MS–222, tingkat kelangsungan hidup.

Masculinization on tilapia (Oreochromis sp.) by natural hormone steroid agent from bee resin through artificial diet Soelistyowati, Dinar Tri; Sudrajat, Agus Oman; Arfah, Harton
Jurnal Akuakultur Indonesia Vol. 9 No. 2 (2010): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Masculinization on tilapia ( Oreochromis sp.) has been done with approach of natural hormone steroid agent from bee resin through artificial diet before sexual differentiation. This research aim to increase male sex ratio and production performance. Treatment of bee resin dose is 0.6; 1.2; 1.8; 2.4; 3.0 feed ml/kg compared to Control (0 ml/kg diet) were given during 28 days at larva age 7 day in aquarium, then transferred to the farm ground until age 12 weeks. Average of masculine phenotype percentage increased significantly (P

Growth and survival of giant gourami juvenile immersed in freshwater containing growth hormone Ratnawati, Pustika; Alimuddin, .; Arfah, Harton; Sudrajat, Agus Oman
Jurnal Akuakultur Indonesia Vol. 11 No. 2 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Growth of giant gourami is relatively slow. Administration of recombinant growth hormone (rGH) by severaltimes immersion in water containing 0.9% NaCl for one hour can significantly increase the growth. In thisstudy, giant gourami juvenile were immersed in freshwater containing 120 mg/L rGH to determine the longimmersion time that provides higher growth and survival. Juvenile at seventh-day-old after egg yolk absorbedwas immersed for 0.5, 1.0, 2.0, and 3.0 hours in rGH solution. As controls, fish was immersed in freshwaterwithout rGH (control 1: C1), and water containing 0.9% NaCl, and 120 mg/L rGH (control 2: C2). Fish waskept under controlled conditions in 20 L aquarium for three weeks, then in 50 L aquarium until at end ofexperiment, and fed at satiation. The results showed that highest growth in body weight, survival, and biomass(p<0.05) were obtained in rGH immersion treatment for 0.5 hour. Biomass of fish immersed by rGH for 0.5hour was about 32% higher compared to control C1, and 13% compared to C2. Survival of the 0.5 hour rGHimmersedfish (100%) was similar to control C1 and C2. Thus, immersion of giant gourami juvenile infreshwater containing rGH for 0.5 hour allowed highest growth rate, and this method is relatively easy to beadopted by fish hatchery.Keywords: immersion, hormone, growth, freshwater, giant gourami

Co-Authors , Alimuddin . Melati Agus Oman Sudrajat Ahmad Fahrul Syarif Ahmad Habibie, Ahmad Ahmad Muzaki Ahmad Teduh Akbar, Muhamad Saepul Alimuddin Alimuddin . Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin ANNA OCTAVERA Asep Bulkini Dinamella Wahjuningrum Dinar Tri Seolistyowati Dinar Tri Soelistyowati Eddy Supriyono Edison, Thomas Eka Kusuma Eni Kusrini, Eni Eny Heriyati Fahmi Hasan, Fahmi Fajar Maulana . Fitriyah Husnul Khotimah Gloria Ika Satriani Gloria Ika Satriani GORO YOSHIZAKI Gusti Ngurah Permana Hafidah, Riva Halawa, Nenima Hanggara, Yudha Haryanti Haryanti Haryanti Haryanti Hasan Nasrullah Herjayanto, Muh. Indah Mastuti Irma Andriani IRMA ANDRIYANI Irzal Effendi Iskandar, Andri Istifarini, Mita Ita Djuwita Julie Ekasari K. Sumantadinata Ketut Mahardika Ketut Sugama Ketut Sugama Khotimah, Fitriyah Husnul Komar Sumantadinata Komar Sumantadinata Kukuh Nirmala Lilis Mulyati Lina Mulyani M. Zairin Junior Marlina Achmad Maulana, Fajar Mia Setiawati Muhamad Fathurrizki Darmawan MUHAMMAD AGUS SUPRAYUDI Muhammad Zairin Muhammad Zairin Jr Muhammad Zairin Jr. Mulyani, Lina Musthofa, Siti Zuhriyyah Nazar, Danella Austraningsih Puspa Nur Rachmawaty Arma Odang Carman Permana, Andre Pustika Ratnawati Rahmi, Kurnia Anggraini Sandra, Aan Aryanti Sari Budi Moria Sembiring Sari Budi Moria Sembiring Satriani, Gloria I. Sholihin, Hidayatush Sinansari, Shofihar Siska Aliyas Sandra Sri Nuryati Sulistyowati, Dinar Tri Wibowo, Kesit Tisna Wicaksono, Aryo Wenang WIDANARNI WIDANARNI

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