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Induction of maturation gonads the Asian swamp eel Monopterus albus at different sizes with the hormones PMSG + antidopamine and hCG Sandra, Aan Aryanti; Sudrajat, Agus Oman; Arfah, Harton; Soelistyowati, Dinar Tri
Jurnal Akuakultur Indonesia Vol. 24 No. 2 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.2.274-287

Monopterus albus is a protogynous hermaphrodite species that undergoes a unidirectional sex change from female to male. Asian swamp eel cultivation activities face constraints in seeding due to limited mature gonad broodstock. This study aims to evaluate the effect of administering a combination of PMSG + Antidopamine (OodevⓇ) and hCG on the induction of gonad maturation of Asian swamp eels at various body sizes. The research design used was a factorial design consisting of control treatment (without hormone injection), P1 = hCG (dose 20 IU/kg), P2 = OodevⓇ (dose 0.5 ml/kg), and P3 = hCG+OodevⓇ (dose 20 IU+0.5 ml/kg) with body length groups, namely K1 (15–25 cm), K2 (30–40 cm), and K3 (45–55 cm). The results showed that eels in group K1 had the highest body length growth (3.63 ± 0.96 cm), while the highest body weight gain (5.30 ± 1.30 grams) in K3 (P<0.05). Administration of oodevⓇ showed the highest values for GSI = 8.13 ± 0.72% and HSI = 4.36±0.80% in K1 compared to K2 (GSI = 6.95 ± 2.86%; HSI = 3.26 ± 0.68%) with female sex. In contrast, group K3 experienced a decrease in GSI = 0.81 ± 0.11% and HSI = 2.48 ± 1.06% accompanied by masculinization. Administration of oodevⓇ also increased the concentration of estradiol-17β in K1 and K2, while testosterone increased in K3. The conclusion of this study is that administering oodevⓇ to 15-40 cm sized Asian swamp eels can accelerate gonad maturity with female sex status, while at sizes >45 cm it can accelerate masculinization and maturation of male gonads. Keywords: body length size, gonad maturation, masculinization, Monopterus albus, oodevⓇ ABSTRAK Monopterus albus adalah spesies hermaprodit protogini yang mengalami perubahan jenis kelamin dari betina ke jantan secara searah. Kegiatan budidaya belut sawah menghadapi kendala dalam pembenihan karena keterbatasan induk matang gonad. Penelitian ini bertujuan untuk mengevaluasi pengaruh pemberian kombinasi PMSG + Antidopamine (OodevⓇ) dan hCG terhadap induksi pematangan gonad belut sawah pada berbagai ukuran tubuh. Desain penelitian yang digunakan adalah rancangan faktorial yang terdiri dari perlakuan kontrol (tanpa injeksi hormon), P1 = hCG (dosis 20 IU/kg), P2 = OodevⓇ (dosis 0,5 ml/kg), dan P3 = hCG+OodevⓇ (dosis 20 IU+0,5 ml/kg) dengan kelompok ukuran panjang tubuh yaitu K1 (15–25 cm), K2 (30–40 cm), dan K3 (45–55 cm). Hasil penelitian menunjukkan bahwa belut kelompok K1 memiliki pertumbuhan panjang tubuh tertinggi (3,63 ± 0,96 cm), sementara pertambahan bobot tubuh tertinggi (5,30±1,30 gram) pada K3 (P<0,05). Pemberian oodevⓇ menunjukkan nilai tertinggi untuk GSI = 8,13±0,72% dan HSI = 4,36±0,80% pada K1 dibandingkan dengan K2 (GSI = 6,95±2,86%; HSI = 3,26±0,68%) dengan jenis kelamin betina. Sebaliknya, kelompok K3 mengalami penurunan nilai GSI = 0,81±0,11% dan HSI = 2,48±1,06% disertai dengan maskulinisasi. Pemberian oodevⓇ juga meningkatkan konsentrasi estradiol-17β pada K1 dan K2, sedangkan testosterone meningkat pada K3. Kesimpulan penelitian ini adalah pemberian oodevⓇ pada belut sawah ukuran 15-40 cm dapat mempercepat kematangan gonad dengan status kelamin betina sedangkan pada ukuran >45 cm dapat mempercepat maskulinisasi dan pematangan gonad jantan. Kata kunci: maskulinisasi, Monopterus albus, oodevⓇ, pematangan gonad, ukuran panjang tubuh

EDUKASI PROSES REPRODUKSI UNTUK PEMBERDAYAAN PEMBUDIDAYA LELE: UPAYA MENINGKATKAN PRODUKTIVITAS BENIH DI KABUPATEN KEDIRI Alimuddin, Alimuddin; Widanarni, Widanarni; Sudrajat, Agus Oman; Nuryati, Sri; Arfah, Harton; Rahmi, Kurnia Anggraini
Jurnal Abdi Insani Vol 12 No 9 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i9.2864

Catfish (Clarias sp.) aquaculture in Kediri Regency, East Java, faces challenges due to the limited availability of high-quality broodstock and the lack of mastery in catfish spawning technologies, such as broodstock selection and the use of natural materials and probiotics to accelerate gonad maturation. These issues formed the basis for implementing a community empowerment program titled "Empowering Fish Farmers through Reproductive Process Education to Improve Catfish Seed Productivity in Kediri Regency." The program aimed to educate fish farmers on proper catfish breeding techniques, from broodstock selection to the use of probiotics and pine fruit extract to enhance gonad maturation and broodstock rematuration, through outreach activities. The community service program included public outreach to fish farming communities and discussions on program sustainability with the Kediri Regency Fisheries Department. Key stakeholders involved in the program included IPB University, the Fisheries Department of Kediri Regency, fisheries extension officers (Ministry of Marine Affairs and Fisheries), and catfish farmer groups in the region. The outreach began with a pre-test and concluded with a post-test to evaluate the program’s effectiveness. The materials covered included broodstock selection, application of pine fruit extract and probiotics for accelerating gonad maturation and rematuration. The community responded positively, as evidenced by an increase in average test scores from 78.42 (pre-test) to 92.63 (post-test), indicating that participants had gained a general understanding of catfish farming techniques and, through the outreach, comprehended the technology needed to produce high-quality broodstock.

Tingkat Kelangsungan Hidup dan Kinerja Pertumbuhan Benih Ikan Lele (Clarias sp) dengan Pemberian Hormon Serotonin Halawa, Nenima; Sudrajat, Agus Oman; Alimuddin, Alimuddin; Arfah, Harton
Jurnal Intek Akuakultur Vol. 6 No. 2 (2022): Intek Akuakultur
Publisher : Program Studi Budidaya Perairan Universitas Maritim Raja Ali Haji

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (946.39 KB)

Masalah utama yang dihadapi dalam penyediaan benih ikan lele yang berkualitas adalah rendahnya tingkat kelangsungan hidup dan pertumbuhan. Adapun upaya yang dapat dilakukan untuk meningkatkan tingkat kelangsungan hidup ikan lele dan pertumbuhan dengan melalui pemberian hormon serotonin. Tujuan dari penelitian ini untuk mengevaluasi peran dan dosis hormon serotonin terhadap tingkat kelulusan hidup dan kinerja pertumbuhan pada benih ikan lele. Penelitian ini menggunakan RAL yang terdiri dari 4 perlakuan dengan 3 ulangan yakni pemberian hormon serotonin sebesar 0 (S0), 0.1 (S1), 1.0 (S2), 1.5 (S3) mg /g-1 ikan. Hasil penelitian menunjukkan bahwa pemberian serotonin sebesar 1.0 (S2) mg /g-1 ikan mampu meningkatkan kelulusan hidup benih ikan lele sebesar 8.70 % dan menghambat kinerja pertumbuhan sebesar 13.86 % pada benih ikan lele (Clarias sp).

Selection and cultivation of seaweed Kappaphycus alvarezii using a long line system (outdoor) Satriani, Gloria I.; Soelistyowati, Dinar Tri; Alimuddin, Alimuddin; Arfah, Harton; Effendi, Irzal
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 12, No 3 (2023): DECEMBER 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.3.30211

In outdoor cultivation at Amal Beach, three seeding mass selections were reared for 30 days for each cycle. Each variety had a different DGR %/day (Daily Growth Rate %/day) value at the end of the growing season on G1-G2-G3 ranging from 3.62-5.90 %/day, which were still categorized as good, and G3 var.Lampung had the highest DGR value. Carrageenan yield values in this study, G1 to G3, ranged from 23.16% to 45.87%, where the G3 G2 G1 treatment. The best thallus samples (20% were selected for each generation) resulted in carrageenan yield sample population in each variety, where var.Lampung var.Tarakan var.Kupang. The biplot analysis shows that the DGR variable vector coincides with the IAA, Phosphate, Gibberellin, and Ammonia vectors. Ammonia stepwise regression analysis (sig) affects DGR with an Ammonia coefficient value of 4.89; meaning that for each increase in the Ammonia value by one unit, the estimated mean value of DGR will increase by 4.89. Thus, the application of themassbased on the results of this study was able to increase the DGR valueKappaphycus alvarezii,which in turn produce the highest yield of carrageenan withK. alvareziivar.Lampung G3 was the best seed candidate to be developed at the seaweed cultivation site in Tarakan City.Keywords:AlgaePhytohormonesCarrageenanNitrogenViscosity

DIFFERENT LED LIGHT EFFECT ON GONAD MATURATION AND GENE EXPRESSION IN FEMALE SYNODONTIS BROODSTOCKS (Synodontis sp.) Sinansari, Shofihar; Sudrajat, Agus Oman; Arfah, Harton; Alimuddin, Alimuddin; Kusrini, Eni; Carman, Odang
Indonesian Aquaculture Journal Vol 19, No 2 (2024): (December, 2024)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.19.2.2024.179-187

The study investigated the influence of the different LED light spectrums on the maturation of the gonads in female synodontis broodstock and examined the maturation-related gene expression levels. Female synodontis were exposed to four different LED light spectra, specifically: white, blue, green, and red, for a continuous period of 120 days rearing. This study employed a completely randomized design, consisting of four treatments and five individual fish as replication for each treatment. An analysis was conducted on the gonadosomatic index (GSI), hepatosomatic index (HSI), gonad histology, estradiol levels, kiss2 and gnrh2 mRNA expression levels. The utilization of blue LED light treatment is highly effective in enhancing the reproductive parameters in female synodontis fish. The results show that brain and gonad kiss2 mRNA expression levels are not significantly different (P<0.05), while gnrh2 mRNA expression levels were significantly different (P<0.05) and had the highest expression in the gonads. The results suggest that light exposure can induce changes in the expression levels of kiss2 and gnrh2 mRNA, as well as control reproduction.

MORPHOMETRIC AND MOLECULAR VARIATION OF NEON TETRA FISH (Paracheirodon innesi) FROM BOJONGSARI DISTRICT, INDONESIA Musthofa, Siti Zuhriyyah; Alimuddin, Alimuddin; Arfah, Harton; Sulistyowati, Dinar Tri; Carman, Odang; Kusrini, Eni
Indonesian Aquaculture Journal Vol 19, No 2 (2024): (December, 2024)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.19.2.2024.133-145

Neon tetra, Paracheirodon innesi, is endemic to rivers and streams in southeastern Colombia, eastern Peru, and western Brazil and is commercially traded as aquarium fish in the world. In Indonesia, neon tetras were mass produced in Bojongsari District, Depok, West Java as the centre of neon tetra fish production. Understanding their genetic variation is useful for implementing their selective breeding programs, environmental restoration, and estimating genetic contributions in stocks. The current study aimed to investigate the patterns of morphometric and molecular diversity using randomly amplified polymorphic DNA-polymerase chain reaction techniques among farmed broodstocks of P. innesi in Indonesia. Three populations, namely: the Bojongsari, Curug, and Pondok Petir derived from the Bojongsari District, Depok, west Java, were used in the study. Thirty live fish from each location were analyzed based on 25 truss morphometric characters. Sixty fresh fish samples were obtained for DNA analysis using the RAPD-PCR technique, which uses three random primers. Principal component analysis (PCA) was performed to distinguish morphometric variations among populations. Morphological and molecular analysis displayed a similar result that Bojongsari and Pondok Petir neon tetra fish had high similarities, while Curug neon tetra was distinguished from others. The closest genetic distance was between the Pondok Petir and Curug populations (0.4088), while the farthest genetic distance was between the Curug and Bojongsari populations (0.4138). The results will be useful in developing breeding programs to improve broodstock quality.

Selection and cultivation of seaweed Kappaphycus alvarezii using a long line system (outdoor) Satriani, Gloria I.; Soelistyowati, Dinar Tri; Alimuddin, Alimuddin; Arfah, Harton; Effendi, Irzal
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 12, No 3 (2023): DECEMBER 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.3.30211

In outdoor cultivation at Amal Beach, three seeding mass selections were reared for 30 days for each cycle. Each variety had a different DGR %/day (Daily Growth Rate %/day) value at the end of the growing season on G1-G2-G3 ranging from 3.62-5.90 %/day, which were still categorized as good, and G3 var.Lampung had the highest DGR value. Carrageenan yield values in this study, G1 to G3, ranged from 23.16% to 45.87%, where the G3 G2 G1 treatment. The best thallus samples (20% were selected for each generation) resulted in carrageenan yield sample population in each variety, where var.Lampung var.Tarakan var.Kupang. The biplot analysis shows that the DGR variable vector coincides with the IAA, Phosphate, Gibberellin, and Ammonia vectors. Ammonia stepwise regression analysis (sig) affects DGR with an Ammonia coefficient value of 4.89; meaning that for each increase in the Ammonia value by one unit, the estimated mean value of DGR will increase by 4.89. Thus, the application of themassbased on the results of this study was able to increase the DGR valueKappaphycus alvarezii,which in turn produce the highest yield of carrageenan withK. alvareziivar.Lampung G3 was the best seed candidate to be developed at the seaweed cultivation site in Tarakan City.Keywords:AlgaePhytohormonesCarrageenanNitrogenViscosity

POLA PERTUMBUHAN DAN VARIASI GENETIK BERBASIS DNA MIKROSATELIT DARI TIGA POPULASI IKAN BARAMUNDI Lates calcarifer Khotimah, Fitriyah Husnul; Alimuddin, Alimuddin; Soelistyowati, Dinar Tri; Nuryati, Sri; Arfah, Harton; Sugama, Ketut; Permana, Gusti Ngurah; Sembiring, Sari Budi Moria; Haryanti, Haryanti
Jurnal Riset Akuakultur Vol 18, No 1 (2023): (Maret 2023)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.18.1.2023.49-59

Benih ikan baramundi Lates calcarifer diperoleh dari pemijahan alami dengan jumlah induk terbatas sehingga variabilitas pertumbuhan dan kelangsungan hidup antar-batch menjadi tinggi. Penelitian dilakukan untuk menganalisis pertumbuhan dan mengevaluasi variasi genetik ikan kakap putih populasi Australia, Situbondo dan Lampung hasil domestikasi dan dibudidaya di hatcheri skala rumah tangga (HSRT). Sebanyak 10 ekor ikan barramundi dari setiap populasi digunakan untuk analisis variabilitas genetik dengan dua lokus mikrosatelit, yaitu Lca21 dan Lca32. Selanjutnya, data mikrosatelit diolah menggunakan software genetic analysis in excel (GenAlEx 6.51b2). Hasil yang diperoleh menunjukkan bahwa pertumbuhan panjang dan bobot tubuh serta laju pertumbuhan spesifik ikan baramundi dari Australia lebih tinggi dibandingkan dari Situbondo dan Lampung (P<0,05); sedangkan ikan baramundi dari Situbondo dengan Lampung adalah sama (P>0,05). Jumlah alel setiap lokus ikan baramundi berkisar 2-8 alel dan heterozigositas tertinggi dimiliki oleh ikan barramundi asal Situbondo (0,85), diikuti Lampung (0,65) dan paling rendah dari Australia (0,54). Dari hasil riset tersebut dapat disimpulkan bahwa ketiga populasi ikan baramundi hasil domestikasi dan dipeliharan dalam sistem HSRT memenuhi kelayakan untuk digunakan untuk kegiatan hibridisasi atau membentuk populasi awal (sintetis). Barramundi seeds, Lates calcarifer are usually sourced from natural spawning using a limited number of broodstock. Therefore, the growth and survival rate of these seeds vary greatly between batches. The research was performed to determine the growth pattern and genetic variations of barramundi seed populations produced from domesticated broodstock sourced from Australia, Situbondo, and Lampung and reared in small-scale hatcheries. Ten individuals of barramundi from each population were used for microsatellite analysis using two microsatellite loci, namely: Lca 21 and Lca 32. The resulted microsatellite data was processed using the genetic analysis available in Excel software (GenAlEx 6.51b2). The results showed that the growth in length and body weight as well as the specific growth rate of barramundi seeds produced from Australia broodstock were higher than that of Situbondo and Lampung (P<0.05) while the later two were similar (P>0.05). The number of microsatellite alleles ranged from 2-8 and the highest heterozygosity was obtained by barramundi seeds produced by Situbondo (0.85), followed by Lampung (0.65), dan Australia (0.54) broodstock. From the results of the research, it can be concluded that the three populations of barramundi fish, which were domesticated and reared in the HSRT system, meet the criteria for use in hybridization program or for forming a synthetic population.

EVALUASI TEPUNG KEDELAI SEBAGAI SUMBER FITOESTROGEN DALAM PAKAN TERHADAP TINGKAT KANIBALISME BENIH IKAN LELE (Clarias sp.) Nazar, Danella Austraningsih Puspa; Sudrajat, Agus Oman; Arfah, Harton; Wahjuningrum, Dinamella; Maulana, Fajar
Jurnal Riset Akuakultur Vol 17, No 3 (2022): (September) 2022
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.17.3.2022.145-153

Beberapa upaya yang dilakukan untuk menanggulangi adanya kanibalisme pada ikan adalah dengan pemberian hormon sintesis estradiol-17β dan pemberian asam amino triptofan (bahan baku biosintesis serotonin) dalam pakan. Tujuan penelitian ini adalah untuk mengevaluasi pengaruh pemberian tepung kedelai terhadap tingkat kanibalisme benih ikan lele. Tiga dosis perlakuan penambahan tepung kedelai yaitu: 0 (Kontrol), 50 (TK50), dan 100 g kg-1 pakan (TK100). Terdapat dua perlakuan kontrol yaitu penambahan hormon menggunakan 17α-metiltestosteron 30 mg kg-1 pakan (MT) dan estradiol-17β 50 mg kg-1 pakan (E2). Penelitian ini menggunakan benih ikan lele berukuran 2,90 ± 0,41 cm dengan padat tebar 2000 ekor m-2. Pemeliharaan dilakukan selama 30 hari dengan pemberian pakan dilakukan tiga kali sehari (07.00, 12.00, dan 18.00). Metode yang digunakan dalam penelitian ini adalah rancangan acak lengkap dengan menggunakan lima perlakuan yang masing masing diulang sebanyak tiga kali. Hasil dari penelitian ini menunjukkan bahwa penambahan TK100 pada pakan dapat meningkatkan tingkat kelangsungan hidup, menekan adanya kanibalisme serta ikan yang berpotensi kanibal (P<0,05). Hasil dari kinerja pertumbuhan menunjukkan bahwa nilai laju bobot mutlak, laju panjang mutlak, laju panjang spesifik, dan koefisien keragaman panjang memiliki hasil yang berbeda nyata antarperlakuan (P<0,05) dan perlakuan laju bobot spesifik tidak berbeda nyata (P>0,05). Penambahan tepung kedelai dalam pakan mampu menekan adanya kanibalisme pada benih ikan lele sebesar 21,21%. Penurunan kanibalisme tersebut sejalan dengan adanya peningkatan kelangsungan hidup pada benih. Suplementasi tepung kedelai dalam pakan dapat menjadi alternatif solusi untuk penurunan tingkat kanibalisme pada pemeliharaan benih ikan lele.Several attempts have been made to reduce cannibalism in fish by supplementing the synthetic hormone estradiol-17β and amino acid tryptophan (raw material for serotonin biosynthesis) in feed. This study aimed to evaluate the effect of soybean meal on the level of cannibalism of catfish fingerlings. Three treatment doses of the supplementation of soybean meal were 0 (Control), 50 (TK50), and 100 g kg-1 feed (TK100). There were two control treatments, with the addition of hormones using 17α-methyltestosterone 30 mg kg-1 feed (MT) and estradiol-17β 50 mg kg-1 feed (E2). This study used catfish fingerlings measuring 2.90 ± 0.41 cm with a stocking density of 2000 m-2. The experiment was conducted for 30 days, thrice daily feeding (07.00, 12.00, and 18.00). The experiment was arranged in a completely randomized design using five treatments with triplicates. The results show that TK100 produced an increased survival rate and suppressed cannibalism level and potentially cannibalistic fish (P<0.05). The growth performance results show that the total weight rate, relative length rate, specific length rate, and length variation coefficient of catfish fingerlings were significantly different among the treatments (P<0.05). However, the specific weight rate of catfish fish fingerlings was not significantly different among the treatments (P>0.05). The supplementation of soybean meal in feed suppresses cannibalism in the catfish seeds by 21.21%. The decrease in cannibalism was strongly correlated with the increase in the fingerlings’ survival rate. Supplementing soybean meal in feed can be an alternative solution to reduce cannibalism in catfish seed rearing.

INDUKSI HORMONAL PADA SEX REVERSAL UDANG GALAH (Macrobrachium rosenbergii) MENGGUNAKAN EKSTRAK SERBUK SARI PINUS (Pinus tabulaeformis) Arfah, Harton; Soelistyowati, Dinar Tri; Sholihin, Hidayatush; Wibowo, Kesit Tisna; Sudrajat, Agus Oman; Carman, Odang; Maulana, Fajar; Alimuddin, Alimuddin
Jurnal Riset Akuakultur Vol 19, No 2 (2024): Juni (2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.2.2024.85-95

Udang galah (Macrobrachium rosenbergii) merupakan komoditas perikanan air tawar yang memiliki ciri dimorfisme seksual pada pertumbuhan individu jantan lebih cepat dibanding betina. Pembentukan populasi monoseks jantan potensial dalam budidaya bertujuan untuk meningkatkan keseragaman, memacu pertumbuhan, dan efisiensi produksi. Induksi sex reversal pada pembentukan individu neomale dapat dilakukan secara hormonal menggunakan bahan yang mengandung derivat testosteron, yaitu serbuk sari pinus (Pinus tabulaeformis). Penelitian ini bertujuan untuk mengevaluasi efektivitas ekstrak serbuk sari pinus terhadap performa sex reversal pada udang galah. Ekstrak serbuk sari pinus dengan dosis berbeda (0,0 mL L-1; 0,2 mL L-1; 0,4 mL L-1; dan 0,6 mL L-1) diberikan melalui perendaman selama 24 jam pada larva udang galah sebelum berdiferensiasi kelamin (PL15) berukuran panjang 12 ± 0,3 mm dan bobot 0,02 ± 0,001 g dengan kepadatan 60 individu per L, kemudian dipelihara selama 45 hari dalam kontainer (60 x 40 x 30 cm3) dengan tinggi air 20 cm dan kepadatan 1 individu per L. Perlakuan dosis ekstrak serbuk sari pinus dosis 0,2 mL L-1 menghasilkan nisbah kelamin jantan 46,69 ± 5,36%, sedangkan peningkatan dosis 0,4-0,6 mL L-1 dan kontrol tidak efektif mengarahkan kelamin jantan (35-42%) dengan tingkat kelangsungan hidup udang PL60 berkisar 66-82%. Ciri kelamin sekunder udang galah jantan PL60 berukuran 30-32 mm berupa male gonopore (MG) dan appendix masculine (AM), sedangkan betina memiliki appendix interna (AI) pada kaki renang kedua dan rongga di antara kedua kaki jalan kelima. Aplikasi ekstrak serbuk sari pinus pada dosis maksimal 0,2 mL L-1 dapat mengarahkan kelamin jantan udang galah.Giant freshwater prawn (Macrobrachium rosenbergii) is a freshwater fishery commodity with sexual dimorphism in which male individuals grow faster than females. The establishment of a male monosexual population is expected to increase individual uniformity, growth rate, and production efficiency. Induction of sex reversal in the formation of the pseudomale can be done hormonally using materials containing testosterone derivatives, for example, pine pollen (Pinus tabulaeformis). This study aimed to evaluate the effectiveness of pine pollen extract on the sex reversal performance of giant prawns. Pine pollen extract with different doses (0.0 mL L-1; 0.2 mL L-1; 0.4 mL L-1; and 0.6 mL L-1) was given by immersion during 24 hours in prawns before sexual differentiation (PL15) with a length of 12 ± 0.3 mm and a weight of 0.02 ± 0.001 g with a density of 60 individuals per L, then reared for 45 days in a container (60 x 40 x 30 cm3) with a water height of 20 cm and a density of 1 individual per L. Treatment with a dose of pine pollen extract at a dose of 0.2 mL L-1 resulted in a male sex ratio of 46.69 ± 5.36%, while increasing doses of 0.4-0.6 ml L-1 and the control were not effective in the sex reversal (35-42%) with a survival rate of PL60 ranged from 66-82%. The secondary sexual characteristics of PL60 male giant prawns measuring 30-32 mm are male gonopore (MG) and masculine appendix (AM), while female giant prawns have an internal appendix (AI) on the second swimming leg and the cavity between the fifth swimming leg. This study concludes that the application of pine pollen extract at a maximum dose of 0.2 mL L-1 could directly induced sex reversal of female to male of giant prawns.

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