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All Journal Medical Journal of Indonesia Journal of Biomedicine and Translational Research The Indonesian Biomedical Journal Paediatrica Indonesiana Medical Laboratory Technology Journal Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) JPP Jurnal Kesehatan Poltekkes Palembang Journal of Global Pharma Technology Jurnal Kesehatan
Asmarinah
Department Of Medical Biology, Faculty Of Medicine, Universitas Indonesia
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Comparative Analysis of RNA Extraction Quality Using TRIzol-Based and Non-TRIzol Methods in Endometriosis Lesion Tissue Ocktariyana Ocktariyana; Bertha Octarina; Shungai Mutiara Aini; Refai Refai; Irsan Saleh; Fatimah Usman; Adnan Abadi; Asmarinah Asmarinah
Medical Laboratory Technology Journal Vol. 12 No. 1 (2026): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v12i1.744

Abstract

Ribonucleic acid (RNA) is a crucial molecule in gene expression analysis, yet its isolation from solid tissues, such as endometriotic lesions, poses significant challenges due to complex tissue structure and high RNase activity. TRIzol is a widely utilized reagent for RNA extraction, known for its efficacy in lysing cells and stabilizing RNA. This study aimed to compare the quality of RNA extracts from endometriotic lesion tissue samples using TRIzol-based and non-TRIzol-based methods. A quasi-experimental design was employed, involving a control group and an experimental group, with non-randomized sample allocation. A total of 28 samples were analyzed in both the control and treatment groups. The results demonstrated that TRIzol treatment significantly increased the median RNA concentration from endometriotic lesion tissues, yielding 288.6 ng/μL compared to 118.2 ng/μL in the non-TRIzol group (p=0.001). Furthermore, there was a significant difference between TRIzol and non-TRIzol groups in RNA purity based on the Å260/280 ratio (median: 2.00; p-value:0.024). However, there wasn’t a significant difference in the Å260/230 ratio (median: 2.11; p-value: 0.247). In conclusion, these findings indicate that while TRIzol extraction provides higher RNA yield, the non‑TRIzol method ensures slightly superior sample purity, supporting its reliability for downstream transcriptomic analyses.
MTHFR polymorphisms, inadequate folate intake, and anemia between stunted and non-stunted children in East Lombok, Indonesia Dwi Yanti; Min Kyaw Htet; Paul Haggarty; Umi Fahmida; Asmarinah
Paediatrica Indonesiana Vol. 66 No. 5 (2026): September 2026
Publisher : Indonesian Pediatric Society

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Abstract

Background Folate supports one-carbon metabolism, which is important for DNA synthesis and methylation reactions, but is often under-emphasized in stunting-related nutrition programs. Common methylenetetrahydrofolate reductase (MTHFR) polymorphisms may influence folate metabolism when dietary folate intake is inadequate. Objective To analyze  MTHFR C677T and A1298C polymorphisms, inadequate folate intake, and anemia status between young children with and without stunting in East Lombok, Indonesia. Methods This analysis included 299 children aged 18–23.9 months, consisting of 149 stunted and 150 non-stunted children. Dietary intake was assessed using two non-consecutive 24-hour recalls. MTHFR polymorphisms were genotyped using TaqMan allelic discrimination assays. Hemoglobin was measured using an automated analyzer and logistic regression was used to analyze for factors associated with stunting and anemia. Results Genotype data were available for 299 children. For C677T, 29.1% carried CT/TT, while for A1298C, 55.8% carried AC/CC. Folate intake below the recommended requirement was common in both stunted and non-stunted children (89.3% and 86.0%, respectively), while vitamin B12 inadequacy was uncommon 6.7% for both groups). Anemia, defined as hemoglobin <10.5 g/dL, was more frequent among stunted children (34.9% vs. 22.8%, respectively; P=0.02). MTHFR genotype, folate intake, riboflavin intake, iron intake, age, and sex were not associated with stunting. Birth weight was strongly associated with stunting (P<0.001), while age (P=0.004) and sex (P=0.03) were associated with anemia. MTHFR genotype and folate intake were not directly associated with either stunting or anemia. Conclusion MTHFR C677T CT/TT and A1298C AC/CC carriage and folate intake inadequacy were common but not stunting-specific. The absence of genotype differences and the strong birth weight association suggest an explanatory gap beyond current dietary and genetic indicators, supporting future studies incorporating folate biomarkers, anemia-related pathways, and DNA methylation profiles.
Co-Authors Adnan Abadi Agus Rizal Ardy Hariandy Hamid, Agus Rizal Ardy Hariandy Akmal Taher Alida Harahap Amarila Malik Andon Hestiantoro ANOM BOWOLAKSONO Aria Kekalih Astria Rishel, RIka Aucky Hinting Bertha Octarina Bethy Suryawathy Hernowo Billy Harnaldo Putra, Dini Fatmi, Budi Wiweko Chaidir Arif Mochtar Chatarina Umbul Wahyuni DIAN LESTARI Dwi Yanti Elvira Hinsch Endang W Bachtiar Evi Hasnita Fajriani, Rosalina Fatimah Eliana Fatimah Usman Febri, Ririn Febriyeni Febriyeni, Febriyeni Ferry Safriadi Ferry Sandra Fiastuti Witjaksono Gita Pratama H. J. Freisleben Harahap, Alida Roswita Hartanto Bayuaji Indah S. Widyahening Irsan Saleh Joedo Prihartono Lisnawati M. Refai Muslich Meilania Saraswati Miftahuz Zakiyah Min Kyaw Htet Muhammad I. Saleh Muharam, Muharam Nukman H. Moeloek Nukman Moeloek Nur Rahadiani Nur Rasyid Nurjati C. Siregar Ocktariyana Ocktariyana Ocktariyana, Ocktariyana Octariyana Oentoeng Soeradi Paul Haggarty Petrus Supardi Ponco Birowo Pradana Suwondo Puji Sari Raden Muharam Natadisastra Raden Susworo Retno Asti Werdhani Rizki Amanda, Clara Samsuridjal Djauzi Savira, Missy Septelia I. Wanandi Shungai Mutiara Aini Silvia W Lestari Silvia werdhy lestari Siregar C Siregar, Siregar C Susan Rahayu Syafruddin Ilyas Tjokorda Gde Dalem Pemayun Trinovita Andraini Tulandi, Togas Umi Fahmida Valencia Yuwono Vanny Narita Widi Atmoko Wistara, Sevita Sathya Zuraida