I Gusti Agung Ayu Suartini
Fakultas Kedokteran Hewan, Universitas Udayana

Published : 27 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 27 Documents
Search

Infeksi Alami Canine Parvovirus pada Anjing Kintamani di Desa Sukawana, Kintamani, Bangli, Bali (NATURAL INFECTION OF CANINE PARVOVIRUS IN KINTAMANI DOGS OF SUKAWANA VILLAGE, KINTAMANI, BANGLI, BALI) I Gusti Ayu Agung Suartini; Indrawati Sendow; I Nyoman Suarsana; Ni Luh Eka Setiasih; Maratun Janah
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (182.441 KB) | DOI: 10.19087/jveteriner.2019.20.2.234

Abstract

Kintamani dog as one of germ plasm owned by Bali province has been widely accepted as dog of Indonesian origin which need to be preserved. Report have shown that puppies of Kintamani dogs sold in Denpasar animal market often die due to Canine parvovirus (CPV) infection. The mortality of CPV infection in puppies can reach as high as 91% espescially in unvaccinated dogs. As the mortality of CPV in dogs is very high, it is important to find out the seroprevalence of CPV infection in Kintamani dogs in Sukawana village. Up to now, the seroprevalence of CPV infection in Sukawana, the natural habitate of Kintamani dog has never been reported. In this study the sample collection and area selection was conducted by haemaggutination inhibition (HI) test. Sera sample were concluded positive if the HI titers of sera were > 64 HI units. Seroprevalence of CPV infection was calculated by dividing the number of positive sera with the total sera samples. The seroprevalence of CPV among dogs was determined using non parametric analysis (Chi-Square). From 70 sera samples collected 67.1% (47/70) were antibody positive against CPV. The highest seroprevalence was found in Banjar Sukawana 22.8% (16/70). A higher seroprevalence was found in female dogs 45.7% (32/70) compare to male dogs 21.4% (15/70). Kintamani dogs aged between 724 month have the highest seroprevalence 27.1% (19/70). Based on the distribution of antibody titers, the seroprevalence antibody >64 HI was 65.7%. The result showed that the high titer (> 64 HI) of antibody against CPV, it was shown that CPV infection has occurs naturally in kintamani dog at Sukawana village.
Pakan Tambahan dan Anabolik Growth Promoter Meningkatkan Kadar Hormon Pertumbuhan Sapi Bali Ni Made Riska Adnyani; Ni Ketut Suwiti; I Gusti Ayu Agung Suartini; I Nengah Kerta Besung
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.64 KB) | DOI: 10.19087/jveteriner.2020.21.4.575

Abstract

This study aims to determine the level of growth hormone given feed supplement and anabolic growth promoter. This research was an experimental research using Complete Random Design (CRD) with three factors. There were feed supplement, anabolic growth promoter, and sampling time. There were 20 youngmale Bali cattle and they were taken care intensively, divided into 4 group, there were control, feed supplement, anabolic growth promoter, and the combination of feed supplement and anabolic growth promoter for five months, and measured the level of growth hormone each month. The level of growth hormone was detected by using competitive Enzyme Linked Immunosorbent Assay method. The result of the research showed that feed supplement and anabolic growth promoter increased the level of bali cattle growth hormone where feed supplement with anabolic growth promoter (P1G1) was not significantlydifferent than given feed supplement without anabolic growth promoter (P1G0). Providing feed supplement optimally by farmers is highly recommended.
Aktivitas Hipolipidemik dan Indeks Aterogenik yang Rendah Ekstrak Air Daun Tapak Dara pada Tikus Hiperkolesterolemia (HYPOLIPIDEMIC ACTIVITY AND LOW ATHEROGENIC INDEX OF AQUEOUS LEAF EXTRACTS OF CATHARANTHUS ROSEUS IN HYPERCHOLESTEROLEMIC RATS) I Nyoman Suarsana; Iwan Harjono Utama; I Made Kardena; I Gusti Ayu Agung Suartini; Ni Luh Watiniasih
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (119.36 KB)

Abstract

Catharanthus roseus is one plant recognized as medical potential which can decrease cholesterol. Thepresent study was carried out to evaluate the effect of aqueous leaf extracts of C. roseus on plasma lipidprofil levels in rats cholesterol rich diet and atherogenic index. This study was carried out on 15 SpraqueDawley male rats randomly distributed into five groups (n=3). Rats hypercholesterolemic wereadministrated cholesterol rich diet containing 1% (w/w). Normal control group with normal diet whitoutextracts (K1), positive control hypercholesterolemic group (K2) with cholesterol rich diet whitout extracts,and others three groups (K3-K5) were feed high cholesterol and 1 mL of aqueous leaf extracts of C. roseuswith a dose of 20% (w/v), 40% (w/v) and 80% (w/v) respectively, administered twice daily 1 mL orally.Treatment was given for 28 days. After treatment, the plasma lipids profile such cholesterol total, highdensity lipoprotein (HDL), low density lipoprotein (LDL), triglyceride (TGA) level were measured. Theresults showed that during the period of treatment with cholesterol rich diet have produced under condition hypercholesterolemic of rats with average cholesterol levels of 124.33 ± 4.04 mg/dL (K2 group). Treatmentwith extract dose of 80% showed the best results among the other doses in reducing levels of total cholesterol,triglycerides, LDL and raise HDL levels. Extract treatment dose of 80% up to four weeks resulted incholesterol total (79.33±3.51 mg/dL), TGA (72.33±6.65 mg/dL), HDL (55.00±3.60 mg/dL), and LDL(9.87±5.34mg/dL). In addition, value ratio of cholesterol:HDL was 1.4: 1 and atherogenic index value was0.44. These results suggest that extracts of leaf C. roseus optimum doses 80% (w/v) has hipolipidemicactivity in hypercholesterolemic rats and it has low atherogenic index value.
Bisphenol A Meningkatkan Malondialdehid dan Indeks Apoptosis Hati Tikus (Rattus norvegicus) Jantan Risha Catra Pradhany; I Nyoman Suarsana; I Gusti Ayu Agung Suartini; Ferbian Milas Siswanto
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.285 KB) | DOI: 10.19087/10.19087/jveteriner.2022.23.1.80

Abstract

Bisphenol-A (BPA) merupakan toksikan yang diketahui dampaknya terhadap reproductive toxicities.Namun, beberapa tahun belakangan ini, diketahui pula bahwa BPA menyebabkan stres oksidatif. Stres oksidatif merupakan salah satu faktor penyebab kerusakan organ. Penelitian ini bertujuan untuk membuktikan efek pemberian BPA oral terhadap kadar malondialdehid dan indeks apoptosis pada hati tikus. Penelitian ini menggunakan posttest only control group design. Subjek adalah 14 ekor tikus putih jantan galur Sprague dawley, umur 8-10 minggu, bobot badan berkisar 180 g, dan dalam keadaan sehat. Kelompok kontrol (P0), tujuh ekor tikus, diberikan plasebo berupa 1 mL aquadest selama 21 hari; sedangkan kelompok perlakuan (P1), tujuh ekor tikus, diberikan 400 mg/kgBB tikus BPA selama 21 hari. Hasil menunjukkan bahwa kelompok P1 memiliki kadar MDA hepatik yang lebih tinggi (3,33±0,27 nmol/ mg.prot) dan berbeda nyata (p<0,001) dibandingkan kelompok P0 (2,67±0,14 nmol/mg.prot). Selain itu, kelompok P1 memiliki indeks apoptosis yang lebih tinggi (11,21±2,26%) dan berbeda nyata (p<0,001) dibandingkan kelompok P0 (2,19±0,97%). Berdasarkan hasil tersebut, dapat disimpulkan bahwa pemberian BPA oral meningkatkan malondialdehid dan indeks apoptosis pada hati tikus putih (Rattus norvegicus) jantan.
MOLECULAR MORPHOLOGY OF SNAKE VENOM PROTEIN Trimeresurus insularis AFTER FREEZE DRYING DETECTED BY SCANNING ELECTRON MICROSCOPE Adrianto, Steven; Sari, Tri Komala; Suarsana, I Nyoman; Suartini, I Gusti Ayu Agung
Jurnal Kedokteran Hewan Vol 19, No 1 (2025): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v19i1.41219

Abstract

The high protein content in snake venom make venom samples prone damaged. Freeze drying is a solution to stabilize the protein in snake venom and the addition of excipients such as sugar and surfactant, can prevent negative impacts during freeze drying process. The purpose of this study was to examine the morphology of Trimeresurus insularis (T. insularis) snake venom protein molecules after freeze-drying with the addition of sucrose, Tween 80, and PBS (pH 7.2). In this study, venom from nine T. insularis snakes was used. Before freeze drying, the treated samples were supplemented with sucrose, Tween 80, and PBS (pH 7.2), while, the control sample was only supplemented with PBS (pH 7.2). After freeze drying, the morphology of both samples was observed using a scanning electron microscope at magnification of 100x and 3000x. The result showed that the control sample was damaged and resembled broken glass, whereas the treatment sample, although it also appeared as shattered glass, was more intact and exhibited finer and smaller flakes.
IDENTIFICATION OF GRAM-NEGATIVE BACTERIAL ISOLATES FROM PIG TONSILS BASED ON MORPHOLOGY AND BIOCHEMICAL TEST Yan Agus Dharma Nataswara; I Wayan Suardana; I Gusti Ayu Agung Suartini
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p29

Abstract

Gram-negative bacteria are microorganisms characterized by significant pathogenic potential and are implicated in a wide range of infections affecting both humans and animals. The tonsils, as lymphoid organs integral to the immune system, can act as niches for bacterial colonization due to their conducive microenvironment for microbial growth. This study aimed to identify Gram-negative bacterial species isolated from porcine tonsils through morphological characterization, oxidase testing, and biochemical profiling using the Methyl Red–Voges-Proskauer (MR-VP) tests, as well as to determine the proportional distribution of the identified bacterial taxa. A total of ten presumptive Gram-negative bacterial isolates, preserved in 30% glycerol, were examined at the Veterinary Public Health Laboratory, Faculty of Veterinary Medicine, Udayana University. The isolates underwent Gram staining, oxidase assay, and MR-VP biochemical testing. The results revealed that seven of the ten isolates (70%) were identified as Pseudomonas spp., while the remaining three isolates (30%) belonged to the family Enterobacteriaceae. All isolates tested positive for the MR test, whereas eight isolates were positive and two negative in the VP test. These findings suggest that porcine tonsils may serve as reservoirs for Gram-negative bacteria, particularly Pseudomonas spp. and members of the Enterobacteriaceae family, which may possess zoonotic potential and thus warrant further surveillance and risk assessment.
COMPARISON OF BALB/C MICE IMMUNE RESPONSE WHICH HAS BEEN INDUCED WITH DIFFERENT RABIES VACCINE Putera, Ilham Yusuf Bahniar; Suartini, I Gusti Ayu Agung; Sari, Tri Komala
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2026 Vol. 18 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2026.v18.i01.p01

Abstract

Rabies is a fatal zoonotic disease that remains incurable, making vaccination the primary strategy for prevention in veterinary and human public health. Although multiple commercial rabies vaccines are widely used, comparative data on their immunogenicity are limited and often inconsistent. This study evaluated and compared the humoral and cellular immune responses induced by three commercially available rabies vaccine brands using a BALB/c mouse model. Ten 20-week-old female BALB/c mice were allocated into treatment groups and immunized accordingly. Blood samples were collected at week 0 (pre-vaccination), week 2, and week 4 post-vaccination to determine specific anti-rabies antibody titers using enzyme-linked immunosorbent assay (ELISA). At the end of the study, spleens were harvested for histopathological examination with hematoxylin–eosin staining to assess cellular immune responses. Antibody titers were analyzed using analysis of variance (ANOVA). All three vaccines induced a significant and comparable increase in antibody titers up to two weeks post-vaccination, with no statistically significant differences among brands. By week 4, a decline in antibody levels was observed across all groups. Histopathological analysis revealed similar activation of cellular immunity among vaccines, characterized by lymphoid proliferation and plasma cell activity, without notable inter-group differences. In conclusion, the three commercial rabies vaccines demonstrated equivalent immunogenicity in inducing both humoral and cellular immune responses in BALB/c mice. These findings suggest that no single vaccine brand was superior, and that rabies vaccination strategies should prioritize operational factors such as schedule adherence, booster administration, and proper vaccination techniques rather than brand selection.