Bambang Sapta Purwoko
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (IPB University), Jl. Meranti, Kampus IPB Dramaga, Bogor 16680, Indonesia

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Pengaruh Aplikasi Mikroba terhadap Fisiologis Beberapa Varietas Padi Fase Vegetatif pada Kondisi Salin Tergenang Badrudin, Ubad; Ghulamahdi, Munif; Purwoko, Bambang Sapta; Pratiwi, Etty
Agro Bali : Agricultural Journal Vol 6, No 3 (2023)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v6i3.1279

Abstract

Padi merupakan makanan pokok di Indonesia yang produksinya harus ditingkatkan seiring bertambahnya jumlah penduduk. Lahan produktif beralih fungsi untuk kepentingan nonpertanian yang menyebabkan ketersediaan lahan untuk pertanian semakin sempit. Alternatif solusinya pemanfaatan lahan suboptimal (lahan salin menggenang). Tetapi, lahan ini dapat menghambat pertumbuhan dan produksi tanaman karena tingginya tekanan osmotik dan daya hantar listrik serta rendahnya ketersediaan oksigen yang dibutuhkan tanaman. Salah satu upayanya dengan aplikasi mikroba (pupuk hayati) dan varietas padi adaptif dan toleran. Pupuk hayati mampu memperbaiki kondisi salin menggenang dengan memfiksasi N, melarutkan fosfat dan kalium, memproduksi hormon pertumbuhan. Tujuan penelitian untuk mengetahui pengaruh mikroba terhadap aspek fisiologi beberapa varietas padi pada kondisi salin menggenang. Penelitian berlangsung mulai bulan September-Desember 2022. Perancangan percobaan menggunakan Rancangan Acak Kelompok dengan dua faktor, yaitu jenis konsorsium mikroba (tanpa mikroba/M0; konsorsium mikroba 1/M1; konsorsium mikroba 2/M2; konsorsium mikroba 3/M3), dan varietas padi (Pokkali/V1; Biosalin 2 Agritan/V2; IR 29/V3). Variabel pengamatan tinggi tanaman, jumlah anakan, klorofil (SPAD), jumlah stomata, klorofil a, klorofil b, prolin, enzim SOD, laju fotosintesis, konduktansi stomata, dan laju transpirasi. Data dianalisis dengan uji F, apabila berbeda nyata dilanjutkan dengan uji DMRT 5%. Hasil penelitian menunjukkan konsorsium mikroba berpengaruh sangat nyata terhadap kadar prolin, klorofil a, dan klorofil b, sedangkan varietas padi pada tinggi tanaman, jumlah stomata, dan laju transpirasi berpengaruh sangat nyata,namun tidak terdapat interaksi pada semua variabel pengamatan.
Agronomic Performance and Selection of Doubled-Haploid Rice Lines for Rainfed Lowland Paddy Field da Cunha, Rojino; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Suwarno, Willy Bayuardi
Journal of Tropical Crop Science Vol. 7 No. 03 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.7.03.148-161

Abstract

Rainfed lowland rice cultivation is an alternative to increase national rice production. Breeding of high yielding rice varieties suitable for rainfed lowland condition can be accelerated by using doubled-haploid (DH) as genetic materials. This study aimed at obtaining information on the agronomic performance including yields in several DH rice lines and selecting DH lines suitable for rainfed lowland paddy field. The experimental design used was a Randomized Complete Block Design with 3 replications. The treatment was thirty DH lines and 4 check varieties namely RJ31 Ciherang, RJ32 Inpari 18, RJ33 Inpari 40, and RJ43 Inpari 41. The results showed that there were variability in all agronomic performances, i.e., plant height, number of tillers, days to heading and to harvest, panicle length, number of filled and empty grains, 1000-grain weight and grain yield. The DH lines, namely RJ19 DR8-43-3-1 and RJ25 DR10-14-1-1, gave the same productivity as 4 check varieties. Index selection showed that twelve DH lines with medium number of productive tillers, early maturing, and productivity of more than 4.40 tons.ha-1 were selected for further evaluation.
Respon Genotipe Padi Sawah terhadap Pemupukan Nitrogen Di Dataran Tinggi (Response of Rice Genotypes to Nitrogen Fertilizer in Highland) Limbongan, Yusuf La’lang; Purwoko, Bambang Sapta; Trikoesoemaningtyas, ,; Aswidinnoor, Hajrial
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 3 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.335 KB) | DOI: 10.24831/jai.v37i3.1232

Abstract

The objective of this experiment was to study the response of rice genotypes to nitrogen under low temperature stress condition.  This experiment was done at Sesean rural area, Tana Toraja Regency, South Sulawesi, 1500 m above sea level with mean temperature 18oC. Experiment was designed in Completely Randomized Block Design with two factors.  The first factor comprised of 3 levels of Nitrogen dosage (0, 50, and 100 kg N / ha) and the second factor was cultivar i.e. Pulu' Mandoti, Pinjan, Lambau, Fatmawati, Sintanur and Gilirang.  The results showed that at low temperature stress condition, nitrogen, cultivar and their interaction gave significant effect on percentage of pollen fertility, percentage of filled spikelet and weight of filled grain per spikelet, while interaction between nitrogen and cultivar did not significantly affect the flag leaf length and flowering date. Rice yield decreased with increasing N levels under low temperature stress condition. There were significant positive correlations between growth components (percentage of pollen fertility, flag leaf length and flowering date) and yield components (number of tiller and grain per panicle and weight of 1000 grain).   Key words:  low temperature stress,  nitrogen fertilizer, rice, highland, pollen fertility
Konstribusi Akumulasi Silikat, Nitrogen dan Aluminium terhadap Ketenggangan Aluminium dan Ketahanan terhadap Penyakit Blas pada Padi Gogo Bakhtiar, ,; Purwoko, Bambang Sapta; Trikoesoemaningtyas, ,; Dewi, Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 3 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.15 KB) | DOI: 10.24831/jai.v37i3.1235

Abstract

Aluminum (Al) toxicity and blast disease are the most important yield-limiting factors for upland rice production in acid soils. The objective of this experiment was to examine the contribution of accumulation of Silicate (Si), Nitrogen (N) and Al in plant tissue on Al tolerance and blast disease resistance in upland rice. The experiment was arranged in a split-split plot design with 2 replications. Main plots were randomly assigned to blast treatment (control and inoculation to blast fungi). Subplots were assigned to control box (lime 1.5 AlEC) and another acid soils (no lime) box and sub-subplots were assigned to the tested genotypes. The result of the experiment showed that leaf blast disease resistance in rice cannot be solely explained by Si or N content in shoot tissue. The resistant to leaf blast disease might be attributed by high ratio Si/N weight in shoot. Al tolerance was ascribed by low reduction in root growth, high shoot dry weight, high Si content in shoot, and as well as high of  Si/Al ratio in root.   Key words:  Al-tolerance, blast disease, upland rice, Si/Al ratio
Perbanyakan Vegetatif Tanaman Jarak Pagar (Jatropha curcas L.) dengan Stek Batang: Pengaruh Panjang dan Diameter Stek Santoso, Bambang Budi; Hasnam, ,; Hariyadi, ,; Susanto, Slamet; Purwoko, Bambang Sapta
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 3 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.036 KB) | DOI: 10.24831/jai.v36i3.1385

Abstract

Since physic nut (Jatropha curcas L.) is heterozygous, sexual propagation results in great genotypic and phenotypic variability. Development of efficient techniques for asexual propagation would benefit the nursery industry as this would lead to selection and production of particular clones with desirable characteristics. Therefore, two following studies were conducted from September until December 2007.  The objective was develop a protocol for vegetative propagation of physic nut by stem cutting in different size of cutting. The first experiment was dealt with stem cutting length (20 cm, 25 cm, and 30 cm) at the same size of diameter (2.5-3.0 cm), and the second experiment was dealt with diameter of stem cutting (3 cm, 2.5-2.9 cm, 2.0-2.4 cm, and 1.5-1.9 cm) at the same size of length (30 cm). Each of experiment was designed in Completely Randomized Design with three replications. Each experimental unit consisted of 25 seedlings. The result showed that cutting growth varied depending on length and diameter of stem cutting. However, better seedling growth and better survival of young plant of physic nut can be obtained from stem cutting with 20-30 cm in length and stem cutting with 2.0-2.9 cm in diameter.   Key words: cutting diameter, cutting length, Jatropha, survival, transplanting
Anther Culture Ability from Crossess Between Upland and New Plant Types of Rice Safitri, Heni; Purwoko, Bambang Sapta; Wirnas, Desta; Dewi, Iswari S.; Abdullah, Buang
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 2 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.417 KB) | DOI: 10.24831/jai.v38i2.1790

Abstract

Anther culture provides rapid route in obtaining pure lines in a single generation through producing green haploid plants that may be spontaneously doubled. This technique has been used for crop improvement especially in rice. The objective of this research was to determine regeneration ability of eight F1s derived from crossess between upland and new plant types of rice and from their four parents through anther culture. Completely randomized design with 25 replications was used in this research. Treatments consisted of four parent lines/varieties i.e. P1 (Fatmawati and BP360E-MR-79-2), P2 (Fulan Telo Gawa and Fulan Telo Mihat) and eight F1s obtained from reciprocal crosses of P1 and P2. Callus induction medium was based on N6 medium + 2.0 mg L-1 NAA + 0.5 mg L-1  kinetin + 10-3  M Putrescine, while regeneration medium was based on MS + 0.5 mg L-1  NAA + 2.0 mg L-1  kinetin + 10-3  M Putrescine. The result indicated that F1 derived from Fatmawati x Fulan Telo Gawa (5.00% green plants per total anther) and their reciprocal (3.80% green plants per total anther) crosses were the most responsive genotypes in rice anther culture (had high anther culture ability). The F1 genotypes were more effective to produce green and doubled haploid plants in rice anther culture than their parents. From this research, 161 double haploid plants (29.81%) from total acclimated green plantlets were obtained.  Keywords: anther culture, upland rice, new plant type of rice
Deteksi Dini Toleransi Padi Hibrida terhadap Kekeringan menggunakan PEG 6000 Afa, La Ode; Purwoko, Bambang Sapta; Junaedi, Ahmad; Haridjaja, Oteng; Dewi, Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 1 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (309.329 KB) | DOI: 10.24831/jai.v41i1.7070

Abstract

The objective of this study was to determine a selection method at early stage for drought tolerant hybrid rice. Theexperimental design was split plot with 3 replications. In the first experiment the main plot was concentration of PEG 6000consisting of control and concentration 25% of PEG 6000. The subplots were hybrid genotypes/varieties, i.e. BI485A/BP3,BI485A/BP5, BI485A/BP10, BI485A/BP12, BI485A/BP15, BI599A/BP5, BI599A/BP15, BI665A/BP6, Maro, Hipa 8, IR64and Limboto. In the second experiment the main plot was drought stress level which consisted of control and drought stressat 60% field capacity. The subplots were hybrid genotypes/varieties used in the first experiment. The results showed that PEG 6000 could be used to detect drought tolerant genotypes at early stage. Seedling dry weight was the major character for selecting drought tolerant genotypes. Genotypes BI485A/BP15 and BI559A/BP15 are tolerant to drought and can potentially be grown in rainfed lowland.Keywords: drought tolerant, early selection, hybrid rice, polyethylene glycol
Plant Regeneration of Pummelo cv. Cikoneng from Cotyledon and Epicotyl Saraswati Dewi, Iswari; Rahman, I. H.; Purwoko, Bambang Sapta
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 2 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.88 KB) | DOI: 10.24831/jai.v41i2.7523

Abstract

In vitro conservation needs highly efficient micropropagation protocol. The objective of the research was to obtain an efficient and reproducible protocol for pummelo (Citrus maxima(Burm.) Merr.) micropropagation through direct shoot formation. The experiment was arranged in a completely randomized design with two factors and 20 replications. The 1stfactor was type of explant, i.e. cotyledon and epicotyl segments of Pummelo cv. Cikoneng, while the 2ndfactor was the media composition as follow (1) MS + 1.0 mg BAP L-1 + 0.5 mg Kinetin L-1 + 0.5 mg NAA L-1; (2) MS + 2.0 mg BAP L-1 + 0.5 mg Kinetin L-1 + 0.5 mg NAA L-1; (3) MS + 1.0 mg BAP L-1 + 1.0 mg Kinetin L-1 + 0.5 mg NAA L-1; (4) MS + 2.0 mg BAP L-1 + 0.5 mg Kinetin L-1+ 1.0 mg NAA L-1; (5) MS + 2.0 mg BAP L-1+ 1.0 mg Kinetin L-1 + 1.0 mg NAA L-1. Observation was conducted on days to shoot induction, number of explant forming shoots, shoot height, number of shoots, leaves, and roots. The results showed that adventitious shoots emerged from callus in epicotyl (6-8 WAP), but adventitious shoots could emerge directly without an intervening callus phase from cotyledon (4-5 WAP). Shoots emerged from epicotyl were weak and vitrous due to hyperhydricity, thus they can not be used for micropropagation. Cotyledons cultured in media MS + 1.0 mg BAP L-1 + 0.5 mg Kinetin L-1 + 0.5 mg NAA L-1or media MS + 1.0 mg BAP L-1 + 1.0 mg Kinetin L-1 + 0.5 mg NAA L-1gave the highest percentage of explant forming adventitious shoot (38.8 and 26.3%), highest efficiency of shoot formation (62.5 dan 72.5%), and highest numbers of leaves (value of 1.9 leaves shoot-1) and roots (1.1 roots shoot-1) compared to other media. Since shoot height and number of leaves and root were not significantly different in both media, thus cotyledon and media MS + 1.0 mg BAP L-1 + 0.5 mg Kinetin L-1+ 0.5 mg NAA L-1which was less in Kinetin is suggested to be used for pummelo micropropagation.Keywords: Citrus maxima, epicotyl, cotyledon, regeneration, pummelo
Hubungan Karakter Daun dengan Hasil Padi Varietas Unggul Wahyuti, Titin Budi; Purwoko, Bambang Sapta; Junaedi, Ahmad; ., Sugiyanta; Abdullah, Buang
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 3 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (267.661 KB) | DOI: 10.24831/jai.v41i3.8094

Abstract

Penelitian ditujukan untuk mempelajari  hubungan karakter daun dengan hasil padi varietas unggul, dan dilaksanakandi kebun percobaan Balai Besar Penelitian Tanaman Padi, Muara, Bogor pada bulan Desember 2010 sampai Juni 2011.Percobaan menggunakan rancangan kelompok lengkap teracak dengan empat ulangan dan menggunakan 12 padi varietasunggul sebagai perlakuan. Varietas yang digunakan adalah Rojolele dan Pandan Wangi (varietas unggul lokal/VUL); IR64 dan Ciherang (varietas unggul baru/VUB); Fatmawati, Cimelati, galur BP360 dan B11143 (padi tipe baru/PTB);serta Maro, Rokan, SL-8 SHS, dan PP1 (hibrida). Hasil penelitian menunjukkan Galur B11143, Maro, Cimelati, dan Rokanmemberikan hasil gabah tertinggi. Tingginya hasil berhubungan dengan karakter sudut tiga daun bagian atas, luas daunbendera, kandungan klorofil, dan kandungan gula daun bendera. Karakter sudut tiga daun bagian atas dan luas daunbendera berkorelasi negatif dengan hasil. Kandungan klorofil dan gula daun bendera tahap berbunga dan pengisian bijiberkorelasi positif dengan hasil.Kata kunci: hasil, karakter daun, padi varietas unggul
Potensi dan Stabilitas Hasil, serta Adaptabilitas Galur-galur Padi Gogo Tipe Baru Hasil Kultur Antera ., Purbokurniawan; Purwoko, Bambang Sapta; Wirnas, Desta; Dewi, Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 1 (2014): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1517.935 KB) | DOI: 10.24831/jai.v42i1.8142

Abstract

ABSTRACTThe objectives of the research were to obtain information on yield potential, adaptability and stability of the upland rice lines. Ten lines and two cultivars were planted at five different locations in November 2010-March 2011. In each location, the experimental design was randomized complete block design with four replications. Observation was done on grains weight per hectar. The results showed that FM1R-1-3-1 achieved the highest productivity (5.65 ton ha-1). Genotype FG1R-36-1-1 was classified as stable genotype by four yield stability analysis as followed: Francis-Kannenberg, Finlay-Wilkinson, Eberhart-Russell and AMMI. Genotypes FG1-70-2-1, FG1R-30-1-5, FG1R-30-1-4 and FG1R-30-1-3 were classified as stable genotypes by three yield stability analysis: Finlay-Wilkinson, Eberhart-Russell and AMMI. AMMI  analysis showed that FG1-6-1-2, FG1-65-1-2, FG1R-30-1-1, FM1R-1-3-1, Fat-4-1-1, Situ Bagendit and Towuti as specific genotypes in certain environment.Keywords: dynamic, new plant type, specific, static, yield
Co-Authors , Hariyadi , Hasnam , Susilawati ,, Mawaddah ,, Usman . Djumali . Hariyadi Agus Purwito Agus Zainudin AHMAD JUNAEDI Akbar, Miftahur Rizqi Akhmadi, Gerland Amy Estiati Anggita Duhita Anindyajati Aniversari Apriana Anshori, Muhammad Fuad Ardie, dan Sintho Wahyuning Atmitri Sisharmini Atmitri Sisharmini Awang Maharijaya Azmi, Yudia Bambang Budi Santoso Bayu Pramono Wibowo Branco, Luis Manuel Buang Abdullah CHAIRUL CHAIRUL Cucu Gunarsih Cucu Gunarsih da Cunha, Rojino Daniel Happy Putra Danu Kuncoro Desta Wirnas Dewi, dan Iswari Saraswati DIAN LATIFAH, DIAN DJUMALI DJUMALI DJUMALI DJUMALI, DJUMALI Dwi Guntoro Edi Santosa Efendi, Darda Eko Sulistyono Endah Retno Palupi Eny Widajati Etty Pratiwi, Etty Faiqotul Himma Fitri Fatma Wardani HAJRIAL ASWIDINNOOR Hapsoh Hariyadi Hariyadi Heni Safitri Heni Safitri I NYOMAN RAI I. H. Rahman Indrastuti Apri Rumanti Iskandar Lubis Iswari S. Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Jamhari Jamhari Kartika Kirana Sangga Mara, Kartika Kirana Sangga Kartina, Nita Kurniawan Rudi Trijatmiko Latifah Kosim Darusman Limbongan, Yusuf La’lang Lopes Hornai, Ermelinda Maria Luthfiani, Marisa Vidya Mohamad Rahmad Suhartanto MOHAMMAD CHOLID MOHAMMAD CHOLID, MOHAMMAD Mohammad Syafii Muhamad Syukur Muhamad Yunus Muhammad Cholid Muhammad Jauhar Firdaus Munif Ghulamahdi Nafisah nFN CHAIRUL Nindita, Anggi Nita Kartina, Nita Nuha Hera Putri Nurul Khumaida Oteng Haridjaja Pahlevi, M Reza Purbokurniawan . Purwoko, Bambang Sapta Purwoko Rahman, Rahayu Safitri Redy Gaswanto, Redy Reny Herawati Rina Hapsari Wening, Rina Hapsari Rizkiya, Reynatha Syafira Rizqullah, Ramadaniarto Roedhy Poerwanto ROSIHAN ROSMAN ROSIHAN ROSMAN Rumanti, Indrastuti Apri Safitri, dan Heni Sakhidin Sani, Mutiyara Satoto Satoto Satoto, Satoto SATRIYAS ILYAS Safitri, Heni Sintho Wahyuning Ardie Siti Nurhidayah Siti Yuriyah Slamet Susanto SRI HENDRASTUTI HIDAYAT SRI SETYATI HARJAD SRI SETYATI HARJADI SUDIRMAN YAHYA SUDIRMAN YAHYA Sudirman Yahya SUGENG SUDIATSO SUGENG SUDIATSO Sugiyanta Sugiyanta . Sugiyanta, dan Suhartini, dan Tintin Susilawati Susilawati Syamsiar, Syamsiar TEUKU MUHAMMAD HANAFIAH OELIM Titin Budi Wahyuti Tri Joko Santoso TRI JOKO SANTOSO Trijatmiko, dan Kurniawan Rudi Trikoesoemaningtyas Ubad Badrudin Wibowo, Bayu Pramono Widyastuti, Yuni Willy Bayuardi Suwarno Wospakrik, Andrean Heskiel Yuana, Hayu Widi Yudiansyah Yudiansyah Yuni Widyastuti Yuni Widyastuti Yunus, dan Muhamad Yunus, Muhammad