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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Pembangunan Pedesaan AGRIVITA, Journal of Agricultural Science Caraka Tani: Journal of Sustainable Agriculture Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Agrotek Tropika Journal of Tropical Crop Science Agro Bali: Agricultural Journal Jurnal Penelitian Tanaman Industri (Littri) Annales Bogorienses

Bambang Sapta Purwoko

Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (IPB University), Jl. Meranti, Kampus IPB Dramaga, Bogor 16680, Indonesia

Author-ID : 240446

Agriculture, Biological Sciences & Forestry Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Economics, Econometrics & Finance Industrial & Manufacturing Engineering Other

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Pengaruh Aplikasi Mikroba terhadap Fisiologis Beberapa Varietas Padi Fase Vegetatif pada Kondisi Salin Tergenang Badrudin, Ubad; Ghulamahdi, Munif; Purwoko, Bambang Sapta; Pratiwi, Etty
Agro Bali : Agricultural Journal Vol 6, No 3 (2023)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v6i3.1279

Padi merupakan makanan pokok di Indonesia yang produksinya harus ditingkatkan seiring bertambahnya jumlah penduduk. Lahan produktif beralih fungsi untuk kepentingan nonpertanian yang menyebabkan ketersediaan lahan untuk pertanian semakin sempit. Alternatif solusinya pemanfaatan lahan suboptimal (lahan salin menggenang). Tetapi, lahan ini dapat menghambat pertumbuhan dan produksi tanaman karena tingginya tekanan osmotik dan daya hantar listrik serta rendahnya ketersediaan oksigen yang dibutuhkan tanaman. Salah satu upayanya dengan aplikasi mikroba (pupuk hayati) dan varietas padi adaptif dan toleran. Pupuk hayati mampu memperbaiki kondisi salin menggenang dengan memfiksasi N, melarutkan fosfat dan kalium, memproduksi hormon pertumbuhan. Tujuan penelitian untuk mengetahui pengaruh mikroba terhadap aspek fisiologi beberapa varietas padi pada kondisi salin menggenang. Penelitian berlangsung mulai bulan September-Desember 2022. Perancangan percobaan menggunakan Rancangan Acak Kelompok dengan dua faktor, yaitu jenis konsorsium mikroba (tanpa mikroba/M0; konsorsium mikroba 1/M1; konsorsium mikroba 2/M2; konsorsium mikroba 3/M3), dan varietas padi (Pokkali/V1; Biosalin 2 Agritan/V2; IR 29/V3). Variabel pengamatan tinggi tanaman, jumlah anakan, klorofil (SPAD), jumlah stomata, klorofil a, klorofil b, prolin, enzim SOD, laju fotosintesis, konduktansi stomata, dan laju transpirasi. Data dianalisis dengan uji F, apabila berbeda nyata dilanjutkan dengan uji DMRT 5%. Hasil penelitian menunjukkan konsorsium mikroba berpengaruh sangat nyata terhadap kadar prolin, klorofil a, dan klorofil b, sedangkan varietas padi pada tinggi tanaman, jumlah stomata, dan laju transpirasi berpengaruh sangat nyata,namun tidak terdapat interaksi pada semua variabel pengamatan.

Agronomic Performance and Selection of Doubled-Haploid Rice Lines for Rainfed Lowland Paddy Field da Cunha, Rojino; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Suwarno, Willy Bayuardi
Journal of Tropical Crop Science Vol. 7 No. 03 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.7.03.148-161

Rainfed lowland rice cultivation is an alternative to increase national rice production. Breeding of high yielding rice varieties suitable for rainfed lowland condition can be accelerated by using doubled-haploid (DH) as genetic materials. This study aimed at obtaining information on the agronomic performance including yields in several DH rice lines and selecting DH lines suitable for rainfed lowland paddy field. The experimental design used was a Randomized Complete Block Design with 3 replications. The treatment was thirty DH lines and 4 check varieties namely RJ31 Ciherang, RJ32 Inpari 18, RJ33 Inpari 40, and RJ43 Inpari 41. The results showed that there were variability in all agronomic performances, i.e., plant height, number of tillers, days to heading and to harvest, panicle length, number of filled and empty grains, 1000-grain weight and grain yield. The DH lines, namely RJ19 DR8-43-3-1 and RJ25 DR10-14-1-1, gave the same productivity as 4 check varieties. Index selection showed that twelve DH lines with medium number of productive tillers, early maturing, and productivity of more than 4.40 tons.ha-1 were selected for further evaluation.

Deteksi Dini Toleransi Padi Hibrida terhadap Kekeringan menggunakan PEG 6000 Afa, La Ode; Purwoko, Bambang Sapta; Junaedi, Ahmad; Haridjaja, Oteng; Dewi, Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 1 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The objective of this study was to determine a selection method at early stage for drought tolerant hybrid rice. Theexperimental design was split plot with 3 replications. In the first experiment the main plot was concentration of PEG 6000consisting of control and concentration 25% of PEG 6000. The subplots were hybrid genotypes/varieties, i.e. BI485A/BP3,BI485A/BP5, BI485A/BP10, BI485A/BP12, BI485A/BP15, BI599A/BP5, BI599A/BP15, BI665A/BP6, Maro, Hipa 8, IR64and Limboto. In the second experiment the main plot was drought stress level which consisted of control and drought stressat 60% field capacity. The subplots were hybrid genotypes/varieties used in the first experiment. The results showed that PEG 6000 could be used to detect drought tolerant genotypes at early stage. Seedling dry weight was the major character for selecting drought tolerant genotypes. Genotypes BI485A/BP15 and BI559A/BP15 are tolerant to drought and can potentially be grown in rainfed lowland.Keywords: drought tolerant, early selection, hybrid rice, polyethylene glycol

Plant Regeneration of Pummelo cv. Cikoneng from Cotyledon and Epicotyl Saraswati Dewi, Iswari; Rahman, I. H.; Purwoko, Bambang Sapta
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 2 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

In vitro conservation needs highly efficient micropropagation protocol. The objective of the research was to obtain an efficient and reproducible protocol for pummelo (Citrus maxima(Burm.) Merr.) micropropagation through direct shoot formation. The experiment was arranged in a completely randomized design with two factors and 20 replications. The 1stfactor was type of explant, i.e. cotyledon and epicotyl segments of Pummelo cv. Cikoneng, while the 2ndfactor was the media composition as follow (1) MS + 1.0 mg BAP L-1 + 0.5 mg Kinetin L-1 + 0.5 mg NAA L-1; (2) MS + 2.0 mg BAP L-1 + 0.5 mg Kinetin L-1 + 0.5 mg NAA L-1; (3) MS + 1.0 mg BAP L-1 + 1.0 mg Kinetin L-1 + 0.5 mg NAA L-1; (4) MS + 2.0 mg BAP L-1 + 0.5 mg Kinetin L-1+ 1.0 mg NAA L-1; (5) MS + 2.0 mg BAP L-1+ 1.0 mg Kinetin L-1 + 1.0 mg NAA L-1. Observation was conducted on days to shoot induction, number of explant forming shoots, shoot height, number of shoots, leaves, and roots. The results showed that adventitious shoots emerged from callus in epicotyl (6-8 WAP), but adventitious shoots could emerge directly without an intervening callus phase from cotyledon (4-5 WAP). Shoots emerged from epicotyl were weak and vitrous due to hyperhydricity, thus they can not be used for micropropagation. Cotyledons cultured in media MS + 1.0 mg BAP L-1 + 0.5 mg Kinetin L-1 + 0.5 mg NAA L-1or media MS + 1.0 mg BAP L-1 + 1.0 mg Kinetin L-1 + 0.5 mg NAA L-1gave the highest percentage of explant forming adventitious shoot (38.8 and 26.3%), highest efficiency of shoot formation (62.5 dan 72.5%), and highest numbers of leaves (value of 1.9 leaves shoot-1) and roots (1.1 roots shoot-1) compared to other media. Since shoot height and number of leaves and root were not significantly different in both media, thus cotyledon and media MS + 1.0 mg BAP L-1 + 0.5 mg Kinetin L-1+ 0.5 mg NAA L-1which was less in Kinetin is suggested to be used for pummelo micropropagation.Keywords: Citrus maxima, epicotyl, cotyledon, regeneration, pummelo

Hubungan Karakter Daun dengan Hasil Padi Varietas Unggul Wahyuti, Titin Budi; Purwoko, Bambang Sapta; Junaedi, Ahmad; ., Sugiyanta; Abdullah, Buang
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 3 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Penelitian ditujukan untuk mempelajari hubungan karakter daun dengan hasil padi varietas unggul, dan dilaksanakandi kebun percobaan Balai Besar Penelitian Tanaman Padi, Muara, Bogor pada bulan Desember 2010 sampai Juni 2011.Percobaan menggunakan rancangan kelompok lengkap teracak dengan empat ulangan dan menggunakan 12 padi varietasunggul sebagai perlakuan. Varietas yang digunakan adalah Rojolele dan Pandan Wangi (varietas unggul lokal/VUL); IR64 dan Ciherang (varietas unggul baru/VUB); Fatmawati, Cimelati, galur BP360 dan B11143 (padi tipe baru/PTB);serta Maro, Rokan, SL-8 SHS, dan PP1 (hibrida). Hasil penelitian menunjukkan Galur B11143, Maro, Cimelati, dan Rokanmemberikan hasil gabah tertinggi. Tingginya hasil berhubungan dengan karakter sudut tiga daun bagian atas, luas daunbendera, kandungan klorofil, dan kandungan gula daun bendera. Karakter sudut tiga daun bagian atas dan luas daunbendera berkorelasi negatif dengan hasil. Kandungan klorofil dan gula daun bendera tahap berbunga dan pengisian bijiberkorelasi positif dengan hasil.Kata kunci: hasil, karakter daun, padi varietas unggul

Potensi dan Stabilitas Hasil, serta Adaptabilitas Galur-galur Padi Gogo Tipe Baru Hasil Kultur Antera ., Purbokurniawan; Purwoko, Bambang Sapta; Wirnas, Desta; Dewi, Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 1 (2014): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTThe objectives of the research were to obtain information on yield potential, adaptability and stability of the upland rice lines. Ten lines and two cultivars were planted at five different locations in November 2010-March 2011. In each location, the experimental design was randomized complete block design with four replications. Observation was done on grains weight per hectar. The results showed that FM1R-1-3-1 achieved the highest productivity (5.65 ton ha-1). Genotype FG1R-36-1-1 was classified as stable genotype by four yield stability analysis as followed: Francis-Kannenberg, Finlay-Wilkinson, Eberhart-Russell and AMMI. Genotypes FG1-70-2-1, FG1R-30-1-5, FG1R-30-1-4 and FG1R-30-1-3 were classified as stable genotypes by three yield stability analysis: Finlay-Wilkinson, Eberhart-Russell and AMMI. AMMI analysis showed that FG1-6-1-2, FG1-65-1-2, FG1R-30-1-1, FM1R-1-3-1, Fat-4-1-1, Situ Bagendit and Towuti as specific genotypes in certain environment.Keywords: dynamic, new plant type, specific, static, yield

Penampilan Agronomi dan Uji Toleransi Naungan Galur Dihaploid Padi Gogo Hasil Kultur Antera Mara, Kartika Kirana Sangga; Purwoko, Bambang Sapta; ,, Eko Sulistyono; Dewi, Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 1 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTShifting of upland rice cultivation to marginal land faces drought stress, while planting under young plantation faces shading stress. There should be lines or varieties with good agronomic character and tolerant to the shading stress. Lines used in this experiment were obtained from anther culture, i.e., III3-4-6-1, I5-10-1-1, WI-44, GI-7, O18-b-1, IW-67, IG-19, IG-38, IW-56, B13-2-e. Two experiments were conducted, i.e., agronomic performance experiment and shading tolerance experiment with a quick test method at seedling phase. Batutegi and Way Rarem were used as check varieties in the agronomic experiment. In shading tolerance experiment, Kalimutu was used as sensitive check and Jatiluhur was tolerant check varieties. The results of experiment indicated that B13-2-e (4.64 ton ha-1) and WI-44 line (4.05 ton ha-1) dihaploid lines showed high grain yield and comparable to Way Rarem (4.95 ton ha-1). B13-2-e and WI-44 lines were tolerant to shading. Another tolerant lines to shading, i.e., I5-10-1-1, O18-b-1, and IW-56. B13-2-e and WI-44 lines were recommended to advanced verification on multiple cropping system.Keywords: dry land, multiple cropping

Kultur Antera Hasil Persilangan Padi Lokal Beras Hitam dengan Varietas Budidaya (Fatmawati dan Inpari 13) Azmi, Yudia; Purwoko, Bambang Sapta; Saraswati Dewi, Iswari; Syukur, Muhamad; Suhartini, dan Tintin
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 3 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRAK Development of black rice is quite rapid in Indonesia. The objective of this study was to obtain doubled haploid black rice lines with good agronomic characters through anther culture. Materials used in this experiment were 6 F1 derived from black rice landrace (Melik) and two cultivated varieties (Inpari 13 and Fatmawati): Melik/Inpari13//Inpari13, Melik / Inpari13// Melik, Melik/Fatmawati //Fatmawati///Fatmawati, Melik/ Fatmawati//Fatmawati// /Fatmawati, Melik/Fatmawati, and Melik/Fatmawati//Melik. The experiment was performed using a completely randomized design consisting of 12 replications. An experimental unit was a petri dish containing ± 150 anthers. Results of the anther culture varied widely between crosses. Crosses Melik/Inpari13//Melik, Melik/Fatmawati//Melik, and Melik/Inpari13 //Inpari13 showed the best response on callus induction and plant regeneration compared to the other crosses. Melik / Inpari13 // Melik produced the highest number of acclimatized plants, the highest number and percentage of doubled haploid: 63 plants, 42 plants and 91.3% respectively. The highest number of doubled haploid having black aleuron layer were obtained by Melik/Inpari13//Melik and Melik/Fatmawati//Melik.Keywords: anther culture, black rice, doubled haploid, regeneration

Kultur Antera untuk Mendapatkan Galur Padi Toleran Salinitas Safitri, Heni; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Ardie, dan Sintho Wahyuning
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTHaploid breeding through anther culture allows shortening of the breeding cycle and production of homozygous lines from a segregating population in the immediate generation. This technique has been used for crop improvement especially in rice. The objective of this research was to determine regeneration ability of twelve F1s, derived from reciprocal crossing between high yielding rice variety and rice tolerance to salinity, through anther culture. Completely randomized design with 20 replications was used in this research. Medium for callus induction was based on N6 medium + 2.0 mg NAA L-1 + 0.5 mg kinetin L-1 + 1 mM putrescine, while regeneration medium was based on MS + 0.5 mg NAA L-1 + 2.0 mg kinetin L-1 + 1 mM putrescine. Rooting were done in MS medium + 0.5 mg IBA L-1 + 1 mM putrescine. The result indicated that F1 derived from IR77674/Inpari 29 (3.1% green plants/total anther) was the most responsive genotypes in rice anther culture (high anther culture ability). After greenhouse grow out 125 putative double haploid plants were obtained (41.5% from total acclimated green plantlets). Keywords: double haploid, green planlets, indica rice, salt tolerance

Pembentukan Buah dan Perkecambahan Tanaman Jarak Pagar pada Transformasi Langsung Melalui Jalur Tabung Polen Zainudin, Agus; Purwoko, Bambang Sapta; Santoso, Tri Joko; Ardie, Sintho Wahyuning; Trikoesoemaningtyas, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 46 No. 1 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The genetic transformation via pollen-tube pathway in jatropha is the first alternative method that was applied in this plant. The objective of the research was to study fruit set and germination of three genotypes of jatropha following direct transformations via pollen-tube pathways. The research was conducted during April 2014 until January 2015 at jatropa’s experimental field, Pasuruan, and at green house of ICABIOGRAD, Bogor. Three genotypes of jatropha i.e., IP3A, IP3P and JcUMM18 were used. In the first experiment, split plot design was used where 3 levels of DNA plasmid concentration as a main plot and 5 levels of stigma-drip time of DNA plasmid as a subplot. In the second experiment randomized block design was used with single factor consisting of 15 combinations of concentration and stigma-drip time of DNA plasmid as treatments and control. The results demonstrated that interaction between concentration and application time of DNA plasmid did not significantly affect fruit and seeds formation of three Jatropha genotypes. Combination of DNA plasmid concentration with time of stigma-drip had significant effect on seed germination rate of IP3A genotype, but not significant on the other variables. The concentration of 0.05-0.5 µg µL-1 and application time of DNA plasmid at 1-10 hours after pollination could be applied on jatropha genetic transformation via pollen-tube pathways.Keywords: DNA plasmid, Jatropha curcas, pCAMBIA1301, pollen-tube, stigma-drip

Co-Authors , Hariyadi , Hasnam , Susilawati ,, Mawaddah ,, Usman . Djumali . Hariyadi Agus Purwito Agus Zainudin AHMAD JUNAEDI Akbar, Miftahur Rizqi Akhmadi, Gerland Amy Estiati Anggita Duhita Anindyajati Aniversari Apriana Anshori, Muhammad Fuad Ardie, dan Sintho Wahyuning Atmitri Sisharmini Atmitri Sisharmini Awang Maharijaya Awang Maharijaya Azmi, Yudia Bakhtiar, Bakhtiar. Bambang Budi Santoso Bayu Pramono Wibowo Branco, Luis Manuel Buang Abdullah Buang Abdullah CHAIRUL CHAIRUL Cucu Gunarsih Cucu Gunarsih Cucu Gunarsih da Cunha, Rojino Daniel Happy Putra Danu Kuncoro Desta Wirnas Dewi, dan Iswari Saraswati DIAN LATIFAH, DIAN DJUMALI DJUMALI DJUMALI DJUMALI, DJUMALI Dwi Guntoro Dwi Widyajayantie Edi Santosa Efendi, Darda Eko Sulistyono Endah Retno Palupi Eny Widajati Etty Pratiwi, Etty Faiqotul Himma Fitri Fatma Wardani HAJRIAL ASWIDINNOOR Hapsoh Hariyadi Hariyadi Hayu Widi Yuana Heni Safitri Heni Safitri Heni Saﬁtri I NYOMAN RAI I. H. Rahman Indrastuti Apri Rumanti Iskandar Lubis Iswari S. Dewi Iswari S. Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Jamhari Jamhari Kartika Kirana Sangga Mara, Kartika Kirana Sangga Kartina, Nita Kurniawan Rudi Trijatmiko Latifah Kosim Darusman Lopes Hornai, Ermelinda Maria Luthfiani, Marisa Vidya Maya Ismayati Mohamad Rahmad Suhartanto MOHAMMAD CHOLID MOHAMMAD CHOLID, MOHAMMAD Mohammad Syafii Muhamad Syukur Muhamad Yunus Muhammad Cholid Muhammad Jauhar Firdaus Munif Ghulamahdi Nafisah nFN CHAIRUL Nindita, Anggi Nita Kartina, Nita Nuha Hera Putri Nurul Khumaida Oteng Haridjaja Pahlevi, M Reza Purbokurniawan . Purwoko, Bambang Sapta Purwoko Raden Permana Budi Laksana Rahman, Rahayu Safitri Redy Gaswanto, Redy Reny Herawati Reynatha Syafira Rizkiya Rina Hapsari Wening, Rina Hapsari Rizqullah, Ramadaniarto Roedhy Poerwanto ROSIHAN ROSMAN ROSIHAN ROSMAN Rumanti, Indrastuti Apri Safitri, dan Heni Sakhidin Sani, Mutiyara Satoto Satoto Satoto, Satoto SATRIYAS ILYAS Satya Nugroho Sintho Wahyuning Ardie Sintho Wahyuning Ardie Siti Nurhidayah Siti Yuriyah Slamet Susanto SRI HENDRASTUTI HIDAYAT SRI SETYATI HARJAD SRI SETYATI HARJADI SUDIRMAN YAHYA SUDIRMAN YAHYA Sudirman Yahya SUGENG SUDIATSO SUGENG SUDIATSO Sugiyanta Sugiyanta . Sugiyanta, dan Suhartini, dan Tintin Susilawati Susilawati Takuji Miyamoto TEUKU MUHAMMAD HANAFIAH OELIM Titin Budi Wahyuti Toshiaki Umezawa Tri Joko Santoso TRI JOKO SANTOSO Trijatmiko, dan Kurniawan Rudi Trikoesoemaningtyas Ubad Badrudin Wibowo, Bayu Pramono Widyastuti, Yuni Willy Bayuardi Suwarno Wospakrik, Andrean Heskiel Yudiansyah Yudiansyah Yuni Widyastuti Yuni Widyastuti Yunus, dan Muhamad Yunus, Muhammad Yusuf L. Limbongan

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