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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Pembangunan Pedesaan AGRIVITA, Journal of Agricultural Science Caraka Tani: Journal of Sustainable Agriculture Jurnal Penelitian Pertanian Tanaman Pangan Journal of Tropical Crop Science Agro Bali: Agricultural Journal Jurnal Penelitian Tanaman Industri (Littri)

Bambang Sapta Purwoko

Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (IPB University), Jl. Meranti, Kampus IPB Dramaga, Bogor 16680, Indonesia

Author-ID : 240446

Agriculture, Biological Sciences & Forestry Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Economics, Econometrics & Finance Industrial & Manufacturing Engineering Other

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Resistance of Doubled Haploid Rice Lines with Green Super Rice Characters to Bacterial Leaf Blight Siti Nurhidayah; Bambang Sapta Purwoko; Iswari Saraswati Dewi; Willy Bayuardi Suwarno; Iskandar Lubis; Siti Yuriyah
Caraka Tani: Journal of Sustainable Agriculture Vol 39, No 2 (2024): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v39i2.88198

Bacterial leaf blight (BLB), caused by Xanthomonas oryzae pv. oryzae (Xoo) is a significant disease attacking rice crops worldwide. This disease attacks at various stages of plant growth and causes significant yield loss. Breeding new varieties resistant to BLB is important to support sustainable agriculture in the future. This study aimed to identify new superior green super rice (GSR) lines resistant to BLB disease. The experiment evaluated the resistance of lowland rice lines obtained from anther culture using a factorial randomized complete block design. The 1st factor was genotype, consisting of 20 lines, 2 checks of commercial varieties (Inpari 42 Agritan GSR and Inpari 18), a resistant check (Conde), and a susceptible check (Taichung Native 1). The 2nd factor was BLB pathotypes (i.e., III, IV, and VIII). Quantitative data on disease severity and severity index were analyzed using analysis of variance and t-Dunnett test at 5% level. The results showed that the interaction between genotype and pathotype affected the disease severity and severity index in both growth phases. The tested lines exhibited varying resistance, from susceptible to resistant, to BLB. Four lines (SN 11, 13, 57, and 58) showed moderate to resistant criteria for BLB disease of 3 pathotypes in both growth phases. The selected lines can be used as a source of parents for breeders and candidates for new superior varieties with BLB resistance properties to support the reduction of synthetic chemical bactericide inputs and control BLB disease. However, further field evaluations are necessary to assess their performance.

Analysis of drought stress tolerance in doubled haploid lines of green super rice at the vegetative stage Nurhidayah, Siti; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Suwarno, Willy Bayuardi; Lubis, Iskandar; Hadianto, Wira; Munandar, Arief
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 53 No. 2 (2025): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v53i2.63893

Current climate change has caused drought in various regions, which has decreased rice yields. Green super rice (GSR) has the characteristic of being tolerant to water limitation. GSR lines were bred through anther culture technique to obtain doubled haploid (DH) plants. This study aimed to analyze the response of DH GSR lines to drought stress at the vegetative stage and identify potential lines based on the weighted selection index. The experiment was arranged in a randomized complete block design with three replications. The materials used consisted of twenty DH lines, Inpari 42 Agritan GSR, and Inpari 18 as check varieties, Salumpikit as a drought-tolerant check, and IR 20 as a drought-sensitive check. Qualitative data were analyzed using the non-parametric Friedman test. The result showed different responses among the lines for leaf rolling, leaf drying, and recovery ability. Line SN14 exhibited moderate leaf drying and showed improvement to mild tolerance during the recovery phase. Nine lines (SN12, 14, 32, 40, 51, 57, 58, 59, and 60) were selected using a selection index based on leaf rolling, leaf drying, and recovery ability. These lines can be further tested for drought tolerance tests until the reproductive stage, and the tolerant lines could be useful for future development. Keywords: abiotic stress; anther culture; climate change; selection index; water limitation

Pengaruh Aplikasi Mikroba terhadap Fisiologis Beberapa Varietas Padi Fase Vegetatif pada Kondisi Salin Tergenang Badrudin, Ubad; Ghulamahdi, Munif; Purwoko, Bambang Sapta; Pratiwi, Etty
Agro Bali : Agricultural Journal Vol 6, No 3 (2023)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v6i3.1279

Padi merupakan makanan pokok di Indonesia yang produksinya harus ditingkatkan seiring bertambahnya jumlah penduduk. Lahan produktif beralih fungsi untuk kepentingan nonpertanian yang menyebabkan ketersediaan lahan untuk pertanian semakin sempit. Alternatif solusinya pemanfaatan lahan suboptimal (lahan salin menggenang). Tetapi, lahan ini dapat menghambat pertumbuhan dan produksi tanaman karena tingginya tekanan osmotik dan daya hantar listrik serta rendahnya ketersediaan oksigen yang dibutuhkan tanaman. Salah satu upayanya dengan aplikasi mikroba (pupuk hayati) dan varietas padi adaptif dan toleran. Pupuk hayati mampu memperbaiki kondisi salin menggenang dengan memfiksasi N, melarutkan fosfat dan kalium, memproduksi hormon pertumbuhan. Tujuan penelitian untuk mengetahui pengaruh mikroba terhadap aspek fisiologi beberapa varietas padi pada kondisi salin menggenang. Penelitian berlangsung mulai bulan September-Desember 2022. Perancangan percobaan menggunakan Rancangan Acak Kelompok dengan dua faktor, yaitu jenis konsorsium mikroba (tanpa mikroba/M0; konsorsium mikroba 1/M1; konsorsium mikroba 2/M2; konsorsium mikroba 3/M3), dan varietas padi (Pokkali/V1; Biosalin 2 Agritan/V2; IR 29/V3). Variabel pengamatan tinggi tanaman, jumlah anakan, klorofil (SPAD), jumlah stomata, klorofil a, klorofil b, prolin, enzim SOD, laju fotosintesis, konduktansi stomata, dan laju transpirasi. Data dianalisis dengan uji F, apabila berbeda nyata dilanjutkan dengan uji DMRT 5%. Hasil penelitian menunjukkan konsorsium mikroba berpengaruh sangat nyata terhadap kadar prolin, klorofil a, dan klorofil b, sedangkan varietas padi pada tinggi tanaman, jumlah stomata, dan laju transpirasi berpengaruh sangat nyata,namun tidak terdapat interaksi pada semua variabel pengamatan.

Agronomic Performance and Selection of Doubled-Haploid Rice Lines for Rainfed Lowland Paddy Field da Cunha, Rojino; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Suwarno, Willy Bayuardi
Journal of Tropical Crop Science Vol. 7 No. 03 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.7.03.148-161

Rainfed lowland rice cultivation is an alternative to increase national rice production. Breeding of high yielding rice varieties suitable for rainfed lowland condition can be accelerated by using doubled-haploid (DH) as genetic materials. This study aimed at obtaining information on the agronomic performance including yields in several DH rice lines and selecting DH lines suitable for rainfed lowland paddy field. The experimental design used was a Randomized Complete Block Design with 3 replications. The treatment was thirty DH lines and 4 check varieties namely RJ31 Ciherang, RJ32 Inpari 18, RJ33 Inpari 40, and RJ43 Inpari 41. The results showed that there were variability in all agronomic performances, i.e., plant height, number of tillers, days to heading and to harvest, panicle length, number of filled and empty grains, 1000-grain weight and grain yield. The DH lines, namely RJ19 DR8-43-3-1 and RJ25 DR10-14-1-1, gave the same productivity as 4 check varieties. Index selection showed that twelve DH lines with medium number of productive tillers, early maturing, and productivity of more than 4.40 tons.ha-1 were selected for further evaluation.

Respon Genotipe Padi Sawah terhadap Pemupukan Nitrogen Di Dataran Tinggi (Response of Rice Genotypes to Nitrogen Fertilizer in Highland) Limbongan, Yusuf La’lang; Purwoko, Bambang Sapta; Trikoesoemaningtyas, ,; Aswidinnoor, Hajrial
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 3 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The objective of this experiment was to study the response of rice genotypes to nitrogen under low temperature stress condition. This experiment was done at Sesean rural area, Tana Toraja Regency, South Sulawesi, 1500 m above sea level with mean temperature 18oC. Experiment was designed in Completely Randomized Block Design with two factors. The first factor comprised of 3 levels of Nitrogen dosage (0, 50, and 100 kg N / ha) and the second factor was cultivar i.e. Pulu' Mandoti, Pinjan, Lambau, Fatmawati, Sintanur and Gilirang. The results showed that at low temperature stress condition, nitrogen, cultivar and their interaction gave significant effect on percentage of pollen fertility, percentage of filled spikelet and weight of filled grain per spikelet, while interaction between nitrogen and cultivar did not significantly affect the flag leaf length and flowering date. Rice yield decreased with increasing N levels under low temperature stress condition. There were significant positive correlations between growth components (percentage of pollen fertility, flag leaf length and flowering date) and yield components (number of tiller and grain per panicle and weight of 1000 grain). Key words: low temperature stress, nitrogen fertilizer, rice, highland, pollen fertility

Konstribusi Akumulasi Silikat, Nitrogen dan Aluminium terhadap Ketenggangan Aluminium dan Ketahanan terhadap Penyakit Blas pada Padi Gogo Bakhtiar, ,; Purwoko, Bambang Sapta; Trikoesoemaningtyas, ,; Dewi, Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 3 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Aluminum (Al) toxicity and blast disease are the most important yield-limiting factors for upland rice production in acid soils. The objective of this experiment was to examine the contribution of accumulation of Silicate (Si), Nitrogen (N) and Al in plant tissue on Al tolerance and blast disease resistance in upland rice. The experiment was arranged in a split-split plot design with 2 replications. Main plots were randomly assigned to blast treatment (control and inoculation to blast fungi). Subplots were assigned to control box (lime 1.5 AlEC) and another acid soils (no lime) box and sub-subplots were assigned to the tested genotypes. The result of the experiment showed that leaf blast disease resistance in rice cannot be solely explained by Si or N content in shoot tissue. The resistant to leaf blast disease might be attributed by high ratio Si/N weight in shoot. Al tolerance was ascribed by low reduction in root growth, high shoot dry weight, high Si content in shoot, and as well as high of Si/Al ratio in root. Key words: Al-tolerance, blast disease, upland rice, Si/Al ratio

Perbanyakan Vegetatif Tanaman Jarak Pagar (Jatropha curcas L.) dengan Stek Batang: Pengaruh Panjang dan Diameter Stek Santoso, Bambang Budi; Hasnam, ,; Hariyadi, ,; Susanto, Slamet; Purwoko, Bambang Sapta
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 3 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Since physic nut (Jatropha curcas L.) is heterozygous, sexual propagation results in great genotypic and phenotypic variability. Development of efficient techniques for asexual propagation would benefit the nursery industry as this would lead to selection and production of particular clones with desirable characteristics. Therefore, two following studies were conducted from September until December 2007. The objective was develop a protocol for vegetative propagation of physic nut by stem cutting in different size of cutting. The first experiment was dealt with stem cutting length (20 cm, 25 cm, and 30 cm) at the same size of diameter (2.5-3.0 cm), and the second experiment was dealt with diameter of stem cutting (3 cm, 2.5-2.9 cm, 2.0-2.4 cm, and 1.5-1.9 cm) at the same size of length (30 cm). Each of experiment was designed in Completely Randomized Design with three replications. Each experimental unit consisted of 25 seedlings. The result showed that cutting growth varied depending on length and diameter of stem cutting. However, better seedling growth and better survival of young plant of physic nut can be obtained from stem cutting with 20-30 cm in length and stem cutting with 2.0-2.9 cm in diameter. Key words: cutting diameter, cutting length, Jatropha, survival, transplanting

Anther Culture Ability from Crossess Between Upland and New Plant Types of Rice Saﬁtri, Heni; Purwoko, Bambang Sapta; Wirnas, Desta; Dewi, Iswari S.; Abdullah, Buang
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 2 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Anther culture provides rapid route in obtaining pure lines in a single generation through producing green haploid plants that may be spontaneously doubled. This technique has been used for crop improvement especially in rice. The objective of this research was to determine regeneration ability of eight F1s derived from crossess between upland and new plant types of rice and from their four parents through anther culture. Completely randomized design with 25 replications was used in this research. Treatments consisted of four parent lines/varieties i.e. P1 (Fatmawati and BP360E-MR-79-2), P2 (Fulan Telo Gawa and Fulan Telo Mihat) and eight F1s obtained from reciprocal crosses of P1 and P2. Callus induction medium was based on N6 medium + 2.0 mg L-1 NAA + 0.5 mg L-1 kinetin + 10-3 M Putrescine, while regeneration medium was based on MS + 0.5 mg L-1 NAA + 2.0 mg L-1 kinetin + 10-3 M Putrescine. The result indicated that F1 derived from Fatmawati x Fulan Telo Gawa (5.00% green plants per total anther) and their reciprocal (3.80% green plants per total anther) crosses were the most responsive genotypes in rice anther culture (had high anther culture ability). The F1 genotypes were more effective to produce green and doubled haploid plants in rice anther culture than their parents. From this research, 161 double haploid plants (29.81%) from total acclimated green plantlets were obtained. Keywords: anther culture, upland rice, new plant type of rice

Deteksi Dini Toleransi Padi Hibrida terhadap Kekeringan menggunakan PEG 6000 Afa, La Ode; Purwoko, Bambang Sapta; Junaedi, Ahmad; Haridjaja, Oteng; Dewi, Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 1 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The objective of this study was to determine a selection method at early stage for drought tolerant hybrid rice. Theexperimental design was split plot with 3 replications. In the first experiment the main plot was concentration of PEG 6000consisting of control and concentration 25% of PEG 6000. The subplots were hybrid genotypes/varieties, i.e. BI485A/BP3,BI485A/BP5, BI485A/BP10, BI485A/BP12, BI485A/BP15, BI599A/BP5, BI599A/BP15, BI665A/BP6, Maro, Hipa 8, IR64and Limboto. In the second experiment the main plot was drought stress level which consisted of control and drought stressat 60% field capacity. The subplots were hybrid genotypes/varieties used in the first experiment. The results showed that PEG 6000 could be used to detect drought tolerant genotypes at early stage. Seedling dry weight was the major character for selecting drought tolerant genotypes. Genotypes BI485A/BP15 and BI559A/BP15 are tolerant to drought and can potentially be grown in rainfed lowland.Keywords: drought tolerant, early selection, hybrid rice, polyethylene glycol

Plant Regeneration of Pummelo cv. Cikoneng from Cotyledon and Epicotyl Saraswati Dewi, Iswari; Rahman, I. H.; Purwoko, Bambang Sapta
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 2 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

In vitro conservation needs highly efficient micropropagation protocol. The objective of the research was to obtain an efficient and reproducible protocol for pummelo (Citrus maxima(Burm.) Merr.) micropropagation through direct shoot formation. The experiment was arranged in a completely randomized design with two factors and 20 replications. The 1stfactor was type of explant, i.e. cotyledon and epicotyl segments of Pummelo cv. Cikoneng, while the 2ndfactor was the media composition as follow (1) MS + 1.0 mg BAP L-1 + 0.5 mg Kinetin L-1 + 0.5 mg NAA L-1; (2) MS + 2.0 mg BAP L-1 + 0.5 mg Kinetin L-1 + 0.5 mg NAA L-1; (3) MS + 1.0 mg BAP L-1 + 1.0 mg Kinetin L-1 + 0.5 mg NAA L-1; (4) MS + 2.0 mg BAP L-1 + 0.5 mg Kinetin L-1+ 1.0 mg NAA L-1; (5) MS + 2.0 mg BAP L-1+ 1.0 mg Kinetin L-1 + 1.0 mg NAA L-1. Observation was conducted on days to shoot induction, number of explant forming shoots, shoot height, number of shoots, leaves, and roots. The results showed that adventitious shoots emerged from callus in epicotyl (6-8 WAP), but adventitious shoots could emerge directly without an intervening callus phase from cotyledon (4-5 WAP). Shoots emerged from epicotyl were weak and vitrous due to hyperhydricity, thus they can not be used for micropropagation. Cotyledons cultured in media MS + 1.0 mg BAP L-1 + 0.5 mg Kinetin L-1 + 0.5 mg NAA L-1or media MS + 1.0 mg BAP L-1 + 1.0 mg Kinetin L-1 + 0.5 mg NAA L-1gave the highest percentage of explant forming adventitious shoot (38.8 and 26.3%), highest efficiency of shoot formation (62.5 dan 72.5%), and highest numbers of leaves (value of 1.9 leaves shoot-1) and roots (1.1 roots shoot-1) compared to other media. Since shoot height and number of leaves and root were not significantly different in both media, thus cotyledon and media MS + 1.0 mg BAP L-1 + 0.5 mg Kinetin L-1+ 0.5 mg NAA L-1which was less in Kinetin is suggested to be used for pummelo micropropagation.Keywords: Citrus maxima, epicotyl, cotyledon, regeneration, pummelo

Co-Authors , Hariyadi , Hasnam , Susilawati ,, Mawaddah ,, Usman . Djumali . Hariyadi Agus Purwito Agus Zainudin AHMAD JUNAEDI Akbar, Miftahur Rizqi Akhmadi, Gerland Amy Estiati Anggita Duhita Anindyajati Aniversari Apriana Anshori, Muhammad Fuad Ardie, dan Sintho Wahyuning Atmitri Sisharmini Atmitri Sisharmini Awang Maharijaya Azmi, Yudia Bambang Budi Santoso Bayu Pramono Wibowo Branco, Luis Manuel Buang Abdullah CHAIRUL CHAIRUL Cucu Gunarsih Cucu Gunarsih da Cunha, Rojino Daniel Happy Putra Danu Kuncoro Desta Wirnas Dewi, dan Iswari Saraswati DJUMALI DJUMALI DJUMALI DJUMALI, DJUMALI Dwi Guntoro Edi Santosa Eko Sulistyono Endah Retno Palupi Eny Widajati Etty Pratiwi, Etty Faiqotul Himma HAJRIAL ASWIDINNOOR Hapsoh Hariyadi Hariyadi Heni Safitri Heni Safitri I NYOMAN RAI I. H. Rahman Indrastuti Apri Rumanti Iskandar Lubis Iskandar Lubis Iswari S. Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Jamhari Jamhari Kartika Kirana Sangga Mara, Kartika Kirana Sangga Kartina, Nita Kurniawan Rudi Trijatmiko Latifah Kosim Darusman Limbongan, Yusuf La’lang Lopes Hornai, Ermelinda Maria MOHAMMAD CHOLID MOHAMMAD CHOLID, MOHAMMAD Mohammad Syafii Muhamad Syukur Muhamad Yunus Muhammad Cholid Muhammad Jauhar Firdaus Munandar, Arief Munif Ghulamahdi Nafisah nFN CHAIRUL Nindita, Anggi Nita Kartina, Nita Nuha Hera Putri Nurul Khumaida Oteng Haridjaja Purbokurniawan . Purwoko, Bambang Sapta Purwoko Rahman, Rahayu Safitri Redy Gaswanto, Redy Reny Herawati Rina Hapsari Wening, Rina Hapsari Roedhy Poerwanto ROSIHAN ROSMAN ROSIHAN ROSMAN Rumanti, Indrastuti Apri Safitri, dan Heni Sakhidin Sani, Mutiyara Satoto Satoto Satoto, Satoto SATRIYAS ILYAS Saﬁtri, Heni Sintho Wahyuning Ardie Siti Nurhidayah Siti Nurhidayah Siti Yuriyah Slamet Susanto SRI HENDRASTUTI HIDAYAT SRI SETYATI HARJAD SRI SETYATI HARJADI SUDIRMAN YAHYA Sudirman Yahya SUDIRMAN YAHYA SUGENG SUDIATSO SUGENG SUDIATSO Sugiyanta Sugiyanta . Sugiyanta, dan Suhartini, dan Tintin Susilawati Susilawati Syamsiar, Syamsiar TEUKU MUHAMMAD HANAFIAH OELIM Titin Budi Wahyuti Tri Joko Santoso TRI JOKO SANTOSO Trijatmiko, dan Kurniawan Rudi Trikoesoemaningtyas Ubad Badrudin Wibowo, Bayu Pramono Widyastuti, Yuni Willy Bayuardi Suwarno Wira Hadianto, Wira Yuni Widyastuti Yuni Widyastuti Yunus, dan Muhamad Yunus, Muhammad

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