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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Pembangunan Pedesaan AGRIVITA, Journal of Agricultural Science Caraka Tani: Journal of Sustainable Agriculture Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Agrotek Tropika Journal of Tropical Crop Science Agro Bali: Agricultural Journal Jurnal Penelitian Tanaman Industri (Littri) Annales Bogorienses

Bambang Sapta Purwoko

Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (IPB University), Jl. Meranti, Kampus IPB Dramaga, Bogor 16680, Indonesia

Author-ID : 240446

Agriculture, Biological Sciences & Forestry Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Economics, Econometrics & Finance Industrial & Manufacturing Engineering Other

Published : 66 Documents Claim Missing Document

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Characterization of Doubled Haploid Derived from Anther Culture for New Type Upland Rice Reny Herawati; Bambang Sapta Purwoko; Iswari S. Dewi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 3 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Anther culture is one of tissue culture methods which can be applied to plant breeding programs in order to accelerate the process of obtaining pure lines. The successful development of rice varieties is highly dependent on genetic diversity and desirable traits. To obtain the genetic variability of doubled haploid lines through anther culture techniques, anther F1 or F2 were used as explants sources. The objectives of the study were to select and characterize doubled haploid lines of upland rice having the characters of new plant type, and to study the genetic variability and agronomic characters of tested doubled haploid lines. A total of 58 doubled haploid lines, and four parental lines i.e. Fatmawati, SGJT-28, SGJT-36, and Way Rarem were used in this study. The experiment used completely randomized design with three replications. Results showed that the characters of the doubled haploid lines vary considerably. Selection of the character i.e. number of productive tillers, number of fi lled grain per panicle, and percentage of empty grain was more effective to be selected because they were well correlated to weight of grain per hill, possessed high heritability values, and have wide genetic variability. Based on productive tillers number, number of fi lled grains per panicle, fertility, weight of 1,000 grains, and weight of grains per hill, the lines of P3-26, P3-27, P3-28, P4-45, P5-50, P6-103, P6-105, P3-120, P3-134, P3-135, P3-150, P3-158, P3-248, P3-249, P6-271, P6-272, P6-274, P6-276, and P6-295 were potential for further selection for new type of upland rice.

Keragaan Varietas dan Galur Padi Tipe Baru Indonesia dalam Sistem Ratun , Susilawati; Bambang Sapta Purwoko; Hajrial Aswidinnoor; Edi Santosa
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 3 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Eighteen Indonesian new plant type (NPT) of rice varieties and lines were evaluated in a ratoon system. The results showed that ratooning ability was related to the vegetative growth prior to harvest, which gave high ability ratoon growth. New plant type varieties and several lines of rice had better vegetative growth and produced higher ratoon than other genotypes. Ratoons started to develop 2-7 days after harvest, with an average number of leaf 2-4 per tiller. Number of productive tillers of ratoon ranged from 6.0 to 30.0 per hill, with number of grain per panicle ranged from 38.0 to 228.2, and grain weight per hill of ratoon 10.4 to 31.2 g, dependent on genotypes. Maturity of ratoon was also dependent on genotypes. On average, maturity was 68 days after harvest of the main crop. Grouping of ratooning ability based on hierarchical cluster analysis resulted in three groups, namely nine genotypes had high potential, fi ve genotypes had medium potential, and four genotypes had low potential. This study indicated that ratoon is potential to increase rice planting index in Indonesia. The genotypes with high ratoon ability need to be evaluated for agronomic performance in the fi eld. Keywords: new plant type, ratooning ability, rice

Morpho-Agronomic Characterization and Yield Evaluation of Doubled Haploid Eggplant (Solanum melongena L.) Lines Derived from Anther Culture Reynatha Syafira Rizkiya; Bambang Sapta Purwoko; Awang Maharijaya; Iswari Saraswati Dewi
Caraka Tani: Journal of Sustainable Agriculture Vol 40, No 4 (2025): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v40i4.95406

The improvement of eggplant yield through breeding is crucial. Doubled haploid technology has accelerated the development of varieties. This study utilized doubled haploid anther culture-derived lines (confirmed through flow cytometry and morphology) developed from embryos from a previous study to evaluate the morpho-agronomic performance and yield of doubled haploid eggplant lines. The experiment used a randomized complete block design (RCBD), three replications, and one factor, namely genotype (35 doubled haploid lines, three F1 varieties). Observation was made on plant height, dichotomous height, stem diameter, days to flowering and harvesting, fruit length, fruit diameter, weight per fruit, number of fruits, and fruit yield per plant. The data were analyzed using ANOVA, t-Dunnett, Tukey-Kramer at a 5% level, Pearson correlation coefficient, and selection index. The results showed significant variability in the population of doubled haploid lines. Fruit yield was positively correlated with plant height, dichotomous height, stem diameter, days to flowering, fruit length, and weight per fruit, but negatively correlated with the number of fruits per plant. These variables can be used as selection criteria because of their high heritability and genotypic coefficients of variation. The selection index revealed that the high-yielding doubled haploid lines with desirable morpho-agronomic traits were RS-P2, RS-P6, RS-P9, RS-P14, RS-P18, RS-H19, RS-H20, RS-H23, RS-H27, RS-H3, RS-M31, RS-M32, RS-M33, RS-M34, and RS-M37. The selected lines with high yield and good quality fruit, similar to the commercial hybrid parent, were Hitavi’s derived lines. All selected lines serve as the genetic basis for production improvements and long-term breeding programs for sustainable and productive eggplants that benefit farmers.

Resistance of Doubled Haploid Rice Lines with Green Super Rice Characters to Bacterial Leaf Blight Siti Nurhidayah; Bambang Sapta Purwoko; Iswari Saraswati Dewi; Willy Bayuardi Suwarno; Iskandar Lubis; Siti Yuriyah
Caraka Tani: Journal of Sustainable Agriculture Vol 39, No 2 (2024): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v39i2.88198

Bacterial leaf blight (BLB), caused by Xanthomonas oryzae pv. oryzae (Xoo) is a significant disease attacking rice crops worldwide. This disease attacks at various stages of plant growth and causes significant yield loss. Breeding new varieties resistant to BLB is important to support sustainable agriculture in the future. This study aimed to identify new superior green super rice (GSR) lines resistant to BLB disease. The experiment evaluated the resistance of lowland rice lines obtained from anther culture using a factorial randomized complete block design. The 1st factor was genotype, consisting of 20 lines, 2 checks of commercial varieties (Inpari 42 Agritan GSR and Inpari 18), a resistant check (Conde), and a susceptible check (Taichung Native 1). The 2nd factor was BLB pathotypes (i.e., III, IV, and VIII). Quantitative data on disease severity and severity index were analyzed using analysis of variance and t-Dunnett test at 5% level. The results showed that the interaction between genotype and pathotype affected the disease severity and severity index in both growth phases. The tested lines exhibited varying resistance, from susceptible to resistant, to BLB. Four lines (SN 11, 13, 57, and 58) showed moderate to resistant criteria for BLB disease of 3 pathotypes in both growth phases. The selected lines can be used as a source of parents for breeders and candidates for new superior varieties with BLB resistance properties to support the reduction of synthetic chemical bactericide inputs and control BLB disease. However, further field evaluations are necessary to assess their performance.

Yield Trial of Doubled-Haploid Rice Lines with Multiple Abiotic Stress Tolerance Hayu Widi Yuana; Bambang Sapta Purwoko; Willy Bayuardi Suwarno; Iswari Saraswati Dewi; Cucu Gunarsih
Caraka Tani: Journal of Sustainable Agriculture Vol 40, No 3 (2025): July
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v40i3.94856

Rice production is facing a decline due to climate change and land conversion. The development of high-yielding and multi-tolerant abiotic stress rice varieties (such as drought, salinity, and flooding) is needed for adaptation to a changing climate. This research aimed to identify potential doubled-haploid (DH) rice lines with good agronomic performance and high yield through index selection. The study was conducted in the rainy season of 2024, and used a randomized complete block design with a single genotype factor and three replications. The genetic material evaluated consisted of 56 DH rice lines and 5 check varieties, i.e., Inpari 18, Inpari 30, Inpari 34, Inpari 35, and Ciherang. Observation was conducted on plant height (PH), number of vegetative tillers (NVT), number of productive tillers (NPT), days to 50% flowering (DF), days to harvesting (DTH), number of filled grains (NFG), total grains per panicle (TG), panicle length (PL), and productivity. The results showed high heritability in all observed variables, indicating that the variables could be used as selection criteria. The weighted index selection involving PH, NPT, DTH, and productivity resulted in 30 DH rice lines with good agronomic traits such as medium PH, medium NPT, medium maturity, and productivity above the check varieties. Those selected DH lines can be evaluated further in more diverse environments to study the effects of genotype, environment, and G×E interactions.

Lignocellulosic Characterizations of Indonesian Rice and The Expression Analyses of OsCAD2 and OsMYB55/61 Related To Lignin Biosynthesis Dwi Widyajayantie; Sintho Wahyuning Ardie; Bambang Sapta Purwoko; Raden Permana Budi Laksana; Maya Ismayati; Takuji Miyamoto; Toshiaki Umezawa; Satya Nugroho
Annales Bogorienses Vol. 26 No. 2 (2022): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2022.v26.n2.1-12

Plant cell walls as sources of lignocellulosic biomass are abundant and used in diverse applications. Lignocellulosic contents manipulation can be applied to develop biomass plants. This study aimed to determine the lignocellulosic components, expressions patterns of OsCAD2 and OsMYB55/61 related to lignin biosynthesis, and deposition patterns of lignin in previously determined three low and three high total lignin content rice accessions based on TGA method. The total Klason and acid-soluble lignin showed variations between 18.03-24.53%, consistent with the TGA method. The ash content of the low lignin content (12.50-21.68%) were significantly differed from the high lignin content (24.84-27.91%) accessions. The extractive, α-cellulose, and hemicellulose contents in each accession showed almost similar proportion. Py-GC-MS proved that the S/G ratio and the syringyl contents were directly proportional to the total lignin contents. OsCAD2 expression levels at the heading stages were consistent with their total lignin contents and their lignin histochemical assay results in the vascular bundle and cortical fiber, suggesting its important roles in rice lignin biosynthesis. However, OsMYB55/61 showed inconsistent expression patterns to total lignin contents, which may also upregulate not only lignin biosynthesis but also cellulose biosynthesis.

Co-Authors , Hariyadi , Hasnam , Susilawati ,, Mawaddah ,, Usman . Djumali . Hariyadi Agus Purwito Agus Zainudin AHMAD JUNAEDI Akbar, Miftahur Rizqi Akhmadi, Gerland Amy Estiati Anggita Duhita Anindyajati Aniversari Apriana Anshori, Muhammad Fuad Ardie, dan Sintho Wahyuning Atmitri Sisharmini Atmitri Sisharmini Awang Maharijaya Awang Maharijaya Azmi, Yudia Bakhtiar, Bakhtiar. Bambang Budi Santoso Bayu Pramono Wibowo Branco, Luis Manuel Buang Abdullah Buang Abdullah CHAIRUL CHAIRUL Cucu Gunarsih Cucu Gunarsih Cucu Gunarsih da Cunha, Rojino Daniel Happy Putra Danu Kuncoro Desta Wirnas Dewi, dan Iswari Saraswati DIAN LATIFAH, DIAN DJUMALI DJUMALI DJUMALI DJUMALI, DJUMALI Dwi Guntoro Dwi Widyajayantie Edi Santosa Efendi, Darda Eko Sulistyono Endah Retno Palupi Eny Widajati Etty Pratiwi, Etty Faiqotul Himma Fitri Fatma Wardani HAJRIAL ASWIDINNOOR Hapsoh Hariyadi Hariyadi Hayu Widi Yuana Heni Safitri Heni Safitri Heni Saﬁtri I NYOMAN RAI I. H. Rahman Indrastuti Apri Rumanti Iskandar Lubis Iswari S. Dewi Iswari S. Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Jamhari Jamhari Kartika Kirana Sangga Mara, Kartika Kirana Sangga Kartina, Nita Kurniawan Rudi Trijatmiko Latifah Kosim Darusman Lopes Hornai, Ermelinda Maria Luthfiani, Marisa Vidya Maya Ismayati Mohamad Rahmad Suhartanto MOHAMMAD CHOLID MOHAMMAD CHOLID, MOHAMMAD Mohammad Syafii Muhamad Syukur Muhamad Yunus Muhammad Cholid Muhammad Jauhar Firdaus Munif Ghulamahdi Nafisah nFN CHAIRUL Nindita, Anggi Nita Kartina, Nita Nuha Hera Putri Nurul Khumaida Oteng Haridjaja Pahlevi, M Reza Purbokurniawan . Purwoko, Bambang Sapta Purwoko Raden Permana Budi Laksana Rahman, Rahayu Safitri Redy Gaswanto, Redy Reny Herawati Reynatha Syafira Rizkiya Rina Hapsari Wening, Rina Hapsari Rizqullah, Ramadaniarto Roedhy Poerwanto ROSIHAN ROSMAN ROSIHAN ROSMAN Rumanti, Indrastuti Apri Safitri, dan Heni Sakhidin Sani, Mutiyara Satoto Satoto Satoto, Satoto SATRIYAS ILYAS Satya Nugroho Sintho Wahyuning Ardie Sintho Wahyuning Ardie Siti Nurhidayah Siti Yuriyah Slamet Susanto SRI HENDRASTUTI HIDAYAT SRI SETYATI HARJAD SRI SETYATI HARJADI Sudirman Yahya SUDIRMAN YAHYA SUDIRMAN YAHYA SUGENG SUDIATSO SUGENG SUDIATSO Sugiyanta Sugiyanta . Sugiyanta, dan Suhartini, dan Tintin Susilawati Susilawati Takuji Miyamoto TEUKU MUHAMMAD HANAFIAH OELIM Titin Budi Wahyuti Toshiaki Umezawa TRI JOKO SANTOSO Tri Joko Santoso Trijatmiko, dan Kurniawan Rudi Trikoesoemaningtyas Ubad Badrudin Wibowo, Bayu Pramono Widyastuti, Yuni Willy Bayuardi Suwarno Wospakrik, Andrean Heskiel Yudiansyah Yudiansyah Yuni Widyastuti Yuni Widyastuti Yunus, dan Muhamad Yunus, Muhammad Yusuf L. Limbongan

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