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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Pembangunan Pedesaan AGRIVITA, Journal of Agricultural Science Caraka Tani: Journal of Sustainable Agriculture Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Agrotek Tropika Journal of Tropical Crop Science Agro Bali: Agricultural Journal Jurnal Penelitian Tanaman Industri (Littri)

Bambang Sapta Purwoko

Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (IPB University), Jl. Meranti, Kampus IPB Dramaga, Bogor 16680, Indonesia

Author-ID : 240446

Agriculture, Biological Sciences & Forestry Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Economics, Econometrics & Finance Industrial & Manufacturing Engineering Other

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Penampilan Agronomi dan Uji Toleransi Naungan Galur Dihaploid Padi Gogo Hasil Kultur Antera Mara, Kartika Kirana Sangga; Purwoko, Bambang Sapta; ,, Eko Sulistyono; Dewi, Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 1 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTShifting of upland rice cultivation to marginal land faces drought stress, while planting under young plantation faces shading stress. There should be lines or varieties with good agronomic character and tolerant to the shading stress. Lines used in this experiment were obtained from anther culture, i.e., III3-4-6-1, I5-10-1-1, WI-44, GI-7, O18-b-1, IW-67, IG-19, IG-38, IW-56, B13-2-e. Two experiments were conducted, i.e., agronomic performance experiment and shading tolerance experiment with a quick test method at seedling phase. Batutegi and Way Rarem were used as check varieties in the agronomic experiment. In shading tolerance experiment, Kalimutu was used as sensitive check and Jatiluhur was tolerant check varieties. The results of experiment indicated that B13-2-e (4.64 ton ha-1) and WI-44 line (4.05 ton ha-1) dihaploid lines showed high grain yield and comparable to Way Rarem (4.95 ton ha-1). B13-2-e and WI-44 lines were tolerant to shading. Another tolerant lines to shading, i.e., I5-10-1-1, O18-b-1, and IW-56. B13-2-e and WI-44 lines were recommended to advanced verification on multiple cropping system.Keywords: dry land, multiple cropping

Kultur Antera Hasil Persilangan Padi Lokal Beras Hitam dengan Varietas Budidaya (Fatmawati dan Inpari 13) Azmi, Yudia; Purwoko, Bambang Sapta; Saraswati Dewi, Iswari; Syukur, Muhamad; Suhartini, dan Tintin
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 3 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRAK Development of black rice is quite rapid in Indonesia. The objective of this study was to obtain doubled haploid black rice lines with good agronomic characters through anther culture. Materials used in this experiment were 6 F1 derived from black rice landrace (Melik) and two cultivated varieties (Inpari 13 and Fatmawati): Melik/Inpari13//Inpari13, Melik / Inpari13// Melik, Melik/Fatmawati //Fatmawati///Fatmawati, Melik/ Fatmawati//Fatmawati// /Fatmawati, Melik/Fatmawati, and Melik/Fatmawati//Melik. The experiment was performed using a completely randomized design consisting of 12 replications. An experimental unit was a petri dish containing ± 150 anthers. Results of the anther culture varied widely between crosses. Crosses Melik/Inpari13//Melik, Melik/Fatmawati//Melik, and Melik/Inpari13 //Inpari13 showed the best response on callus induction and plant regeneration compared to the other crosses. Melik / Inpari13 // Melik produced the highest number of acclimatized plants, the highest number and percentage of doubled haploid: 63 plants, 42 plants and 91.3% respectively. The highest number of doubled haploid having black aleuron layer were obtained by Melik/Inpari13//Melik and Melik/Fatmawati//Melik.Keywords: anther culture, black rice, doubled haploid, regeneration

Kultur Antera untuk Mendapatkan Galur Padi Toleran Salinitas Safitri, Heni; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Ardie, dan Sintho Wahyuning
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTHaploid breeding through anther culture allows shortening of the breeding cycle and production of homozygous lines from a segregating population in the immediate generation. This technique has been used for crop improvement especially in rice. The objective of this research was to determine regeneration ability of twelve F1s, derived from reciprocal crossing between high yielding rice variety and rice tolerance to salinity, through anther culture. Completely randomized design with 20 replications was used in this research. Medium for callus induction was based on N6 medium + 2.0 mg NAA L-1 + 0.5 mg kinetin L-1 + 1 mM putrescine, while regeneration medium was based on MS + 0.5 mg NAA L-1 + 2.0 mg kinetin L-1 + 1 mM putrescine. Rooting were done in MS medium + 0.5 mg IBA L-1 + 1 mM putrescine. The result indicated that F1 derived from IR77674/Inpari 29 (3.1% green plants/total anther) was the most responsive genotypes in rice anther culture (high anther culture ability). After greenhouse grow out 125 putative double haploid plants were obtained (41.5% from total acclimated green plantlets). Keywords: double haploid, green planlets, indica rice, salt tolerance

Pembentukan Buah dan Perkecambahan Tanaman Jarak Pagar pada Transformasi Langsung Melalui Jalur Tabung Polen Zainudin, Agus; Purwoko, Bambang Sapta; Santoso, Tri Joko; Ardie, Sintho Wahyuning; Trikoesoemaningtyas, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 46 No. 1 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The genetic transformation via pollen-tube pathway in jatropha is the first alternative method that was applied in this plant. The objective of the research was to study fruit set and germination of three genotypes of jatropha following direct transformations via pollen-tube pathways. The research was conducted during April 2014 until January 2015 at jatropa’s experimental field, Pasuruan, and at green house of ICABIOGRAD, Bogor. Three genotypes of jatropha i.e., IP3A, IP3P and JcUMM18 were used. In the first experiment, split plot design was used where 3 levels of DNA plasmid concentration as a main plot and 5 levels of stigma-drip time of DNA plasmid as a subplot. In the second experiment randomized block design was used with single factor consisting of 15 combinations of concentration and stigma-drip time of DNA plasmid as treatments and control. The results demonstrated that interaction between concentration and application time of DNA plasmid did not significantly affect fruit and seeds formation of three Jatropha genotypes. Combination of DNA plasmid concentration with time of stigma-drip had significant effect on seed germination rate of IP3A genotype, but not significant on the other variables. The concentration of 0.05-0.5 µg µL-1 and application time of DNA plasmid at 1-10 hours after pollination could be applied on jatropha genetic transformation via pollen-tube pathways.Keywords: DNA plasmid, Jatropha curcas, pCAMBIA1301, pollen-tube, stigma-drip

Efficiency of Genetic Transformation via Pollen-Tube Pathway of Jatropha (Jatropha curcas L.) Based on Histochemical and Molecular Analysis Zainudin, Agus; Purwoko, Bambang Sapta; Santoso, Tri Joko; Ardie, Sintho Wahyuning; Trikoesoemaningtyas, and
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 3 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The genetic transformation via pollen-tube pathway is an alternative method to overcome the constraints imposed by genotype specificity in transformation and regeneration in jatropha (Jatropha curcas L.) tissue culture. Therefore, it is necessary to establish important parameters for efficient genetic transformation of jatropha via pollen-tube pathway. The objective of the research was to study the efficiency of direct transformation of jatropha via pollen-tube pathway based on histochemical and molecular analysis. Solution of purified pCAMBIA1301 DNA plasmid carrying a hptII marker gene and a gus reporter gene with concentration level of 0.05, 0.25, 0.50 µg µl-1 were applied to stigma of flowers at 1, 2, 4, 7, 10 h after pollination. Seedling of IP3A, IP3P and JcUMM18 jatropha’s genotypes derived from 15 combination treatments of plasmid DNA concentration and application time, also wild type was subjected to histochemical and molecular analyses. Based on those analyses, the efficiency of transformation via pollen-tube pathway of three jatropha genotypes ranged from 1.5-16.7%. PCR analysis showed that a number of positive plants were identified by using specific primers hptII and gus, i.e. 1-3 and 3-7 plants of the 15 combined treatments, respectively. It indicated that the transformation efficiency via the pollen-tube pathway varied in each jatropha genotype.Keywords: Jatropha curcas L., pCAMBIA1301, plasmid DNA, stigma-drip

Toleransi Galur Harapan Padi Sawah (Oryza sativa L.) pada Persaingan dengan Gulma Echinochloa crus-galli ,, Usman; Purwoko, Bambang Sapta; Syukur, Muhamad; Guntoro, dan Dwi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 2 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTBarnyard grass (Echinochloa crus-galli (L.) P. Beauv.) is a major weed competitor to rice production in Indonesia. In order to develop integrated weed management program, a research to select competitive rice lines to E. crus-galli was conducted in a green house of Indonesian Centre of Agricultural Biotechnology and Genetic Resource Research and Development Bogor. The research design was split plot with four replications, E. crus-galli was designed as the main plot (rice without E. crus-galli compared rice with four E. crus-galli per pot), and the sub-plots were 25 genotypes (23 lines, 1 tolerant variety and 1 sensitive variety). Level of tolerance was determined by the reduction percentage of grains weight, the number of productive tillers and dry matter weight. The results showed that rice competition with E. crus-galli reduced plant height, productive tiller numbers, filled spikelet numbers per panicle, dry matter weight and dry grain weight. Three lines, i.e., IR10L-155, IR10L-133 and BIO-R84-1 were classified as tolerant, 19 lines were moderate and 3 lines were sensitive to E. crus-galli competition.Keywords: Barnyard grass, competition, sensitive line, yield reduction

Pengujian Daya Hasil dan Ketahanan Penyakit Hawar Daun Bakteri Tanaman Padi Hibrida Lopes Hornai, Ermelinda Maria; Purwoko, Bambang Sapta; Suwarno, Willy Bayuardi; Dewi, dan Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 2 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTHybrid rice varieties is an alternative technology to improve productivity of low land rice. The results of previous studies have identified and found the male sterile lines Wild Abortive type and Kalinga are resistant to bacterial leaf blight pathotype III, IV and VIII. The objectives of the research were to obtain information on agronomic characters, yield evaluation, genetic parameters, and repeatability information. The experiment was conducted in two locations namely Muara and Indramayu experimental stations. The design used was a Randomized Complete Block Design with three replication at each site. Each replicate consisted of 17 hybrid rice and three check varieties. The results from locations showed that genotype BI485A/BP1 (IR53942) has the highest yield of 5.8 ton ha-1. The coefficient of genetic and phenotypic diversity of six agronomic characters was low. The repeatability for seven character observed were low, except for days to flowering. The scoring value of bacterial leaf blight disease in Indramayu showed that nine genotypes exhibit resistance.Keywords: cytoplasmic male sterile lines, disease resistance

Regenerasi dan Aklimatisasi Kultur Antera Enam Persilangan F1 Padi Sawah Gunarsih, Cucu; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Syukur, dan Muhamad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 2 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTThe breeding of rainfed rice tolerant to drought can be accomplished using anther culture. The objectives of this research were to determine regeneration abilities of six F1 anther culture and its acclimatization ability. The experiment was arranged in completely randomized design with 14 replications. The treatments consisted of six F1 derived from crossing: INPARI 18 x IR83140-B-11-B (G1), INPARI 18 x B12825E-TB-1-25 (G2), INPARI 18 x IR87705-14-11-B-SKI-12 (G3), INPARI 22 x IR83140-B-11-B (G4), Bio-R81 x O18b-1 (G5), Bio-R82-2 x O18b-1 (G6). Media for callus induction was based on N6 medium + 2.0 mg L-1 NAA + 0.5 mg L-1 kinetin + 1.0 mM putresin + 60 g L-1 sucrosa, media for regeneration was based on MS + 0.5 mg L-1 NAA + 2.0 mg L-1 kinetin + 1.0 mM putresin, and media for rooting was based on MS + 0.5 mg L-1 IBA + 30 g L-1 sucrosa. The result indicated that all six F1 had different ability in anther culture. Bio-R82-2 x O18-b1 (G6) and Bio-R81 x O18-b1 (G5) F1 genotype had good response both of callus induction and plant regeneration. These two F1 genotypes also gave the highest ratio of green planlet production to number of anther inoculated (GP:AI) were 5.50% and 4.65%, respectively. In this research, there were identified doubled haploid plants were developed from 4 F1 derived cross namely G2 (2 plants), G3 (4 plants), G5 (21 plants), and G6 (26 plants).Keywords: Callus induction, doubled haploid, rice

Pemilihan Karakter Agronomi untuk Seleksi pada Galur-galur Padi Dihaploid Hasil Kultur Antera Akhmadi, Gerland; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Wirnas, dan Desta
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 1 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Anther culture technique is able to accelerate plant breeding activities. The objectives of this research was to determine the agronomic traits that could be used for selection of the dihaploid rice line population through the calculation of heritability, genotypic correlation, path analysis and selecting dihaploid rice lines. The plant material used was 65 dihaploid rice lines DH 1 of F1 anther culture plants and Ciherang and Inpari 13 as check varieties. Agronomic characters that could be used as selection character are generative plant height, number of filled grain per panicle, the total number of grains per panicle, weight of 1,000 grains, and grain per hill. Twenty three dihaploid lines were selected based good agronomic characters with criteria generative plant height between 80-120 cm, number of filled grains per panicle > 100, number of grains per panicle > 120, weight of 1,000 grains > 20 g, and grain per hill > 25 g.Keywords: anther culture, heritability, agronomic characters

Identifikasi Toleransi Kekeringan Tetua Padi Hibrida pada Fase Perkecambahan Menggunakan Polietilen Glikol (PEG) 6000 Widyastuti, Yuni; Purwoko, Bambang Sapta; Yunus, dan Muhamad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTDeveloping of drought tolerant hybrid rice varieties requires parental lines which have tolerance to drought. Polyethylene glycol (PEG) has been widely used as an osmotic solution for detecting drought tolerance at germination stage of rice. The objectives of this experiment were to evaluate variables of drought tolerance at germination stage and to select drought tolerance of parental lines using osmotic solution PEG 6000 at concentration of 25%. The experiment was conducted at the greenhouse ICABIOGRAD Cimanggu Bogor, during April-May 2014. The design of the experiment was arranged in factorial randomized block design with three replications. The first factor (genotype) had 21 levels and the second factor (osmotic potential) had two levels (0 and 25% concentration levels of PEG 6000). Based on the principal component analysis, six primary indicators were proposed for drought tolerance of rice at germination stage, i.e., germination percentage, seed vigor, seminal root length, seedling length and dry weight of seminal root. Based on the clustering analysis, at 73.76% of similarity rate, the 21 tested genotypes were divided into 2 groups. The genotypes which showed similar responses with Salumpikit were IR 58025B, GMJ 14B, IR 80154B, GMJ 15B, R 3, PK 90, and PK 12 respectively. Keywords: drought tolerance, germination, hybrid rice PEG, parental lines

Co-Authors , Hariyadi , Hasnam , Susilawati ,, Mawaddah ,, Usman . Djumali . Hariyadi Agus Purwito Agus Zainudin AHMAD JUNAEDI Akbar, Miftahur Rizqi Akhmadi, Gerland Amy Estiati Anggita Duhita Anindyajati Aniversari Apriana Anshori, Muhammad Fuad Ardie, dan Sintho Wahyuning Atmitri Sisharmini Atmitri Sisharmini Awang Maharijaya Azmi, Yudia Bambang Budi Santoso Bayu Pramono Wibowo Branco, Luis Manuel Buang Abdullah CHAIRUL CHAIRUL Cucu Gunarsih Cucu Gunarsih da Cunha, Rojino Daniel Happy Putra Danu Kuncoro Desta Wirnas Dewi, dan Iswari Saraswati DIAN LATIFAH, DIAN DJUMALI DJUMALI DJUMALI DJUMALI, DJUMALI Dwi Guntoro Edi Santosa Efendi, Darda Eko Sulistyono Endah Retno Palupi Eny Widajati Etty Pratiwi, Etty Faiqotul Himma Fitri Fatma Wardani HAJRIAL ASWIDINNOOR Hapsoh Hariyadi Hariyadi Heni Safitri Heni Safitri I NYOMAN RAI I. H. Rahman Indrastuti Apri Rumanti Iskandar Lubis Iswari S. Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Jamhari Jamhari Kartika Kirana Sangga Mara, Kartika Kirana Sangga Kartina, Nita Kurniawan Rudi Trijatmiko Latifah Kosim Darusman Limbongan, Yusuf La’lang Lopes Hornai, Ermelinda Maria Luthfiani, Marisa Vidya Mohamad Rahmad Suhartanto MOHAMMAD CHOLID MOHAMMAD CHOLID, MOHAMMAD Mohammad Syafii Muhamad Syukur Muhamad Yunus Muhammad Cholid Muhammad Jauhar Firdaus Munif Ghulamahdi Nafisah nFN CHAIRUL Nindita, Anggi Nita Kartina, Nita Nuha Hera Putri Nurul Khumaida Oteng Haridjaja Pahlevi, M Reza Purbokurniawan . Purwoko, Bambang Sapta Purwoko Rahman, Rahayu Safitri Redy Gaswanto, Redy Reny Herawati Rina Hapsari Wening, Rina Hapsari Rizkiya, Reynatha Syafira Rizqullah, Ramadaniarto Roedhy Poerwanto ROSIHAN ROSMAN ROSIHAN ROSMAN Rumanti, Indrastuti Apri Safitri, dan Heni Sakhidin Sani, Mutiyara Satoto Satoto Satoto, Satoto SATRIYAS ILYAS Saﬁtri, Heni Sintho Wahyuning Ardie Siti Nurhidayah Siti Yuriyah Slamet Susanto SRI HENDRASTUTI HIDAYAT SRI SETYATI HARJAD SRI SETYATI HARJADI Sudirman Yahya SUDIRMAN YAHYA SUDIRMAN YAHYA SUGENG SUDIATSO SUGENG SUDIATSO Sugiyanta Sugiyanta . Sugiyanta, dan Suhartini, dan Tintin Susilawati Susilawati Syamsiar, Syamsiar TEUKU MUHAMMAD HANAFIAH OELIM Titin Budi Wahyuti Tri Joko Santoso TRI JOKO SANTOSO Trijatmiko, dan Kurniawan Rudi Trikoesoemaningtyas Ubad Badrudin Wibowo, Bayu Pramono Widyastuti, Yuni Willy Bayuardi Suwarno Wospakrik, Andrean Heskiel Yuana, Hayu Widi Yudiansyah Yudiansyah Yuni Widyastuti Yuni Widyastuti Yunus, dan Muhamad Yunus, Muhammad

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